SSGCID: Target Page

NegoA.00980.a

phosphoserine aminotransferase

CENTER ID:	NegoA.00980.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00980.a.B2.GE40038	mature protein		26	426

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00980.a.B2.PS37990	mature protein		26	426

External Resources

RESOURCE	REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYLFVASMIF MFVNQYIDII FRVRAAWNRA EGRYFPSLHK ACCKPKIGRV IVQNQGCIMS LYPIYNFSAG PAVLPEAVLR TAQQEMSDYN GTGFSVMEMS HRSEMFLSIL HHAEQDLRQL LKVPDNYKIL FLQGGATTQF NMAAMNLAHG FRTADAVVTG NWSRIAYEQM SRLTDTEIRL AAHGGEQFDY LDLPPVETWD VAPDSAFVHF AVNETVNGLQ YREVPRLSDG MPPLVCDMSS EILSREFDVA DYGLIYAGAQ KNIGPAGVTV VIVREDLLER CPNDIPDVFN YRSHLNRDGM YNTPSTYAIY MSGLVFRWLQ AQGGVKKIEA VNRLKAQTLY ETIDGSGGFY INDIHPDARS KMNVVFKTAS EDLDRRFVLE AELQGLCLLK GYKSVGGMRA SIYNAMPLEG VRALADFMRD FQRRYG

NT Sequence

ATGTATCTAT TTGTCGCTAG CATGATTTTT ATGTTTGTAA ATCAATATAT TGATATTATT TTCCGTGTTC GGGCGGCATG GAATCGGGCG GAGGGCAGGT ATTTCCCATC CTTGCACAAA GCCTGCTGCA AGCCTAAAAT CGGGCGGGTT ATTGTTCAGA ACCAGGGCTG CATCATGTCT CTTTATCCGA TTTACAATTT TTCCGCCGGC CCTGCCGTGT TGCCTGAAGC TGTGTTGAGG ACGGCGCAGC AGGAAATGTC GGACTACAAC GGTACGGGTT TTTCCGTGAT GGAAATGAGC CACCGTTCGG AAATGTTTTT AAGCATCCTG CATCATGCGG AACAGGATTT GAGGCAGCTT TTGAAAGTGC CTGACAACTA TAAGATATTG TTTCTTCAGG GCGGAGCAAC AACCCAATTT AATATGGCAG CCATGAATCT GGCACACGGT TTCCGCACTG CCGACGCGGT GGTAACGGGC AACTGGAGCC GTATCGCCTA TGAACAGATG AGCCGTTTGA CCGATACGGA AATCCGTTTG GCGGCGCACG GGGGCGAGCA GTTCGACTAT CTCGACCTGC CGCCTGTGGA AACGTGGGAT GTTGCACCCG ATTCGGCGTT TGTCCATTTT GCCGTCAATG AAACTGTCAA CGGGCTGCAA TACCGTGAAG TGCCGCGCCT TTCAGACGGC ATGCCGCCGT TGGTGTGCGA TATGTCCAGC GAGATTCTGT CGCGTGAGTT TGATGTTGCC GACTACGGAC TGATTTACGC AGGCGCACAG AAAAACATCG GGCCGGCAGG AGTTACGGTG GTGATTGTGC GTGAGGATTT GCTCGAGCGT TGTCCGAACG ATATTCCCGA TGTGTTCAAC TACCGTTCTC ATTTGAACCG CGACGGTATG TACAACACGC CGTCAACTTA CGCGATTTAT ATGTCGGGGC TGGTGTTCCG CTGGCTGCAG GCGCAGGGCG GTGTGAAAAA AATTGAAGCG GTCAATCGGC TGAAGGCGCA AACCTTGTAT GAGACGATAG ATGGCAGCGG CGGTTTTTAC ATCAACGATA TTCATCCTGA TGCGCGCTCC AAAATGAACG TGGTCTTCAA AACCGCAAGC GAAGACTTGG ACCGCCGTTT TGTGCTGGAA GCCGAATTGC AGGGCTTGTG CCTGTTGAAG GGCTATAAGT CTGTCGGCGG GATGCGCGCC AGCATTTATA ATGCGATGCC GCTTGAAGGC GTGCGGGCTT TGGCAGATTT TATGCGCGAT TTCCAACGGC GTTACGGTTG A

Details for NegoA.00980.a.B2.GE40038

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA WNRAEGRYFP SLHKACCKPK IGQVIVQNQG CIMSLYPIYN FSAGPAVLPE AVLRTAQQEM SDYNGTGFSV MEMSHRSEMF LSILHHAEQD LRQLLKVPDN YKILFLQGGA TTQFNMAAMN LAHGFRTADA VVTGNWSRIA YEQMSRLTDT EIRLAAHGGE QFDYLDLPPV ETWDVAPDSA FVHFAVNETV NGLQYREVPR LSDGMPPLVC DMSSEILSRE FDVADYGLIY AGAQKNIGPA GVTVVIVRED LLERCPNDIP DVFNYRSHLN RDGMYNTPST YAIYMSGLVF RWLQAQGGVK KIEAVNRLKA QTLYETIDGS GGFYINDIHP DARSKMNVVF KTASEDLDRR FVLEAELQGL CLLKGYKSVG GMRASIYNAM PLEGVRALAD F

Validated NT Sequence

cataaaatct gccaaagccc gcacgccttc aagcggcatc gcattataaa tgctggcgcg catcccgccg acagacttat agcccttcaa caggcacaag ccctgcaatt cggcttccag cacaaaacgg cggtccaagt cttcgcttgc ggttttgaag accacgttca ttttggagcg cgcatcagga tgaatatcgt tgatgtaaaa accgccgctg ccatctatcg tctcatacaa ggtttgcgcc ttcagccgat tgaccgcttc aatttttttc acaccgccct gcgcctgcag ccagcggaac accagccccg acatataaat cgcgtaagtt gacggcgtgt tgtacatacc gtcgcggttc aaatgagaac ggtagttgaa cacatcggga atatcgttcg gacaacgctc gagcaaatcc tcacgcacaa tcaccaccgt aactcctgcc ggcccgatgt ttttctgtgc gcctgcgtaa atcagtccgt agtcggcaac atcaaactca cgcgacagaa tctcgctgga catatcgcac accaacggcg gcatgccgtc tgaaaggcgc ggcacttcac ggtattgcag cccgttgaca gtttcattga cggcaaaatg gacaaacgcc gaatcgggtg caacatccca cgtttccaca ggcggcaggt cgagatagtc gaactgctcg cccccgtgcg ccgccaaacg gatttccgta tcggtcaaac ggctcatctg ttcataggcg atacggctcc agttgcccgt taccaccgcg tcggcagtgc ggaaaccgtg tgccagattc atggctgcca tattaaattg ggttgttgct ccgccctgaa gaaacaatat cttatagttg tcaggcactt tcaaaagctg cctcaaatcc tgttccgcat gatgcaggat gcttaaaaac atttccgaac ggtggctcat ttccatcacg gaaaaacccg taccgttgta gtccgacatt tcctgctgcg ccgtcctcaa cacagcttca ggcaacacgg cagggccggc ggaaaaattg taaatcggat aaagagacat gatgcagccc tggttctgaa caataacctg cccgatttta ggcttgcagc aggctttgtg caaggatgga aaatacctgc cctccgcccg attccatgcc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHAW NRAEGRYFPS LHKACCKPKI GRVIVQNQGC IMSLYPIYNF SAGPAVLPEA VLRTAQQEMS DYNGTGFSVM EMSHRSEMFL SILHHAEQDL RQLLKVPDNY KILFLQGGAT TQFNMAAMNL AHGFRTADAV VTGNWSRIAY EQMSRLTDTE IRLAAHGGEQ FDYLDLPPVE TWDVAPDSAF VHFAVNETVN GLQYREVPRL SDGMPPLVCD MSSEILSREF DVADYGLIYA GAQKNIGPAG VTVVIVREDL LERCPNDIPD VFNYRSHLNR DGMYNTPSTY AIYMSGLVFR WLQAQGGVKK IEAVNRLKAQ TLYETIDGSG GFYINDIHPD ARSKMNVVFK TASEDLDRRF VLEAELQGLC LLKGYKSVGG MRASIYNAMP LEGVRALADF MRDFQRRYG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcatgga atcgggcgga gggcaggtat ttcccatcct tgcacaaagc ctgctgcaag cctaaaatcg ggcgggttat tgttcagaac cagggctgca tcatgtctct ttatccgatt tacaattttt ccgccggccc tgccgtgttg cctgaagctg tgttgaggac ggcgcagcag gaaatgtcgg actacaacgg tacgggtttt tccgtgatgg aaatgagcca ccgttcggaa atgtttttaa gcatcctgca tcatgcggaa caggatttga ggcagctttt gaaagtgcct gacaactata agatattgtt tcttcagggc ggagcaacaa cccaatttaa tatggcagcc atgaatctgg cacacggttt ccgcactgcc gacgcggtgg taacgggcaa ctggagccgt atcgcctatg aacagatgag ccgtttgacc gatacggaaa tccgtttggc ggcgcacggg ggcgagcagt tcgactatct cgacctgccg cctgtggaaa cgtgggatgt tgcacccgat tcggcgtttg tccattttgc cgtcaatgaa actgtcaacg ggctgcaata ccgtgaagtg ccgcgccttt cagacggcat gccgccgttg gtgtgcgata tgtccagcga gattctgtcg cgtgagtttg atgttgccga ctacggactg atttacgcag gcgcacagaa aaacatcggg ccggcaggag ttacggtggt gattgtgcgt gaggatttgc tcgagcgttg tccgaacgat attcccgatg tgttcaacta ccgttctcat ttgaaccgcg acggtatgta caacacgccg tcaacttacg cgatttatat gtcggggctg gtgttccgct ggctgcaggc gcagggcggt gtgaaaaaaa ttgaagcggt caatcggctg aaggcgcaaa ccttgtatga gacgatagat ggcagcggcg gtttttacat caacgatatt catcctgatg cgcgctccaa aatgaacgtg gtcttcaaaa ccgcaagcga agacttggac cgccgttttg tgctggaagc cgaattgcag ggcttgtgcc tgttgaaggg ctataagtct gtcggcggga tgcgcgccag catttataat gcgatgccgc ttgaaggcgt gcgggctttg gcagatttta tgcgcgattt ccaacggcgt tacggttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for NegoA.00980.a.B2.PS37990

PURIFICATION DATe:	7/28/2016
CONCENTRATION:	26.4mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCATGGA ATCGGGCGGA GGGCAGGTAT TTCCCATCCT TGCACAAAGC CTGCTGCAAG CCTAAAATCG GGCGGGTTAT TGTTCAGAAC CAGGGCTGCA TCATGTCTCT TTATCCGATT TACAATTTTT CCGCCGGCCC TGCCGTGTTG CCTGAAGCTG TGTTGAGGAC GGCGCAGCAG GAAATGTCGG ACTACAACGG TACGGGTTTT TCCGTGATGG AAATGAGCCA CCGTTCGGAA ATGTTTTTAA GCATCCTGCA TCATGCGGAA CAGGATTTGA GGCAGCTTTT GAAAGTGCCT GACAACTATA AGATATTGTT TCTTCAGGGC GGAGCAACAA CCCAATTTAA TATGGCAGCC ATGAATCTGG CACACGGTTT CCGCACTGCC GACGCGGTGG TAACGGGCAA CTGGAGCCGT ATCGCCTATG AACAGATGAG CCGTTTGACC GATACGGAAA TCCGTTTGGC GGCGCACGGG GGCGAGCAGT TCGACTATCT CGACCTGCCG CCTGTGGAAA CGTGGGATGT TGCACCCGAT TCGGCGTTTG TCCATTTTGC CGTCAATGAA ACTGTCAACG GGCTGCAATA CCGTGAAGTG CCGCGCCTTT CAGACGGCAT GCCGCCGTTG GTGTGCGATA TGTCCAGCGA GATTCTGTCG CGTGAGTTTG ATGTTGCCGA CTACGGACTG ATTTACGCAG GCGCACAGAA AAACATCGGG CCGGCAGGAG TTACGGTGGT GATTGTGCGT GAGGATTTGC TCGAGCGTTG TCCGAACGAT ATTCCCGATG TGTTCAACTA CCGTTCTCAT TTGAACCGCG ACGGTATGTA CAACACGCCG TCAACTTACG CGATTTATAT GTCGGGGCTG GTGTTCCGCT GGCTGCAGGC GCAGGGCGGT GTGAAAAAAA TTGAAGCGGT CAATCGGCTG AAGGCGCAAA CCTTGTATGA GACGATAGAT GGCAGCGGCG GTTTTTACAT CAACGATATT CATCCTGATG CGCGCTCCAA AATGAACGTG GTCTTCAAAA CCGCAAGCGA AGACTTGGAC CGCCGTTTTG TGCTGGAAGC CGAATTGCAG GGCTTGTGCC TGTTGAAGGG CTATAAGTCT GTCGGCGGGA TGCGCGCCAG CATTTATAAT GCGATGCCGC TTGAAGGCGT GCGGGCTTTG GCAGATTTTA TGCGCGATTT CCAACGGCGT TACGGTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T