SSGCID: Target Page

NegoA.00952.a

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

CENTER ID:	NegoA.00952.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00952.a.B1.GE39980	full length		1	417

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00952.a.B1.PS38219	full length		1	417

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.2507
UniProt:	B4RPR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKLKISANG PLNGEITVSG AKNAALPLMC AGLLTSGTLR LKNVPMLADV ATTQKLLQGM GARVLTDNIS EFEINGGTVN NTCAPYELVR TMRASILVLG PTLARFGEAQ VSLPGGCAIG SRPVNQHLKG LEAMGAEIAI EHGYVKAKGK LKGARVAMDV VTVGGTENLL MAATLAEGTT VLENCAIEPE VVDLAECLVK MGAKISGIGT STMIVEGAGE LYGCEHSVVP DRIEAGTFLC AVAITGGRVV LRNAAPKTME VVLDKLVEAG AVIEAGDDWI AIDMRQRPKA VDIRTVVHPG FPTDMQAQFM ALNAVAEGSC RVVETIFENR FMHVPELNRM GANITTEGNT AFVQGVERLS GAVVKATDLR ASASLVIAGL AARGETVVER IYHLDRGYEN IEKKLGSVGA NIERVSG

NT Sequence

GTGGACAAAC TGAAAATCTC CGCAAACGGC CCGCTCAACG GGGAAATAAC GGTCTCGGGC GCGAAAAACG CGGCATTGCC GCTGATGTGC GCGGGTTTGC TGACATCGGG TACGTTGCGC CTGAAAAACG TCCCTATGCT GGCAGATGTC GCAACCACGC AAAAGCTGCT TCAGGGCATG GGCGCGCGCG TTCTGACCGA CAATATCAGC GAATTTGAAA TCAACGGCGG TACGGTCAAC AATACCTGCG CCCCTTACGA ATTGGTTCGG ACGATGCGCG CTTCGATTTT GGTGCTTGGT CCGACGCTGG CGCGTTTCGG CGAGGCGCAA GTCAGCCTGC CGGGCGGCTG CGCCATCGGT TCGCGCCCCG TCAATCAGCA TTTGAAAGGC TTGGAAGCGA TGGGGGCGGA AATCGCCATC GAACACGGTT ACGTCAAAGC CAAAGGCAAA CTCAAAGGTG CGCGCGTGGC GATGGATGTC GTTACCGTCG GCGGTACGGA AAACCTGCTG ATGGCGGCGA CGCTGGCGGA AGGTACGACG GTTTTGGAAA ACTGCGCCAT CGAGCCTGAA GTGGTCGATT TGGCGGAATG CCTGGTCAAA ATGGGCGCGA AAATCAGCGG CATCGGAACA TCGACCATGA TTGTGGAAGG TGCGGGCGAG CTGTACGGCT GCGAACACAG CGTCGTCCCC GACCGGATTG AAGCGGGGAC GTTCCTGTGT GCGGTGGCGA TAACCGGCGG CAGGGTGGTT TTGCGGAATG CCGCCCCGAA AACGATGGAA GTGGTGTTGG ACAAACTGGT CGAGGCAGGT GCGGTGATTG AAGCGGGCGA CGATTGGATC GCCATCGATA TGCGGCAGCG TCCGAAGGCG GTGGACATCC GCACGGTCGT CCATCCCGGC TTCCCCACGG ATATGCAGGC GCAGTTTATG GCATTGAATG CCGTGGCGGA AGGAAGCTGC CGCGTGGTGG AAACGATTTT TGAAAACCGC TTTATGCACG TTCCGGAGTT GAACCGGATG GGGGCGAACA TTACAACCGA GGGCAATACG GCATTTGTGC AGGGTGTGGA ACGGCTTTCC GGCGCGGTCG TCAAGGCGAC GGATTTGCGC GCGTCCGCCA GCCTAGTTAT CGCCGGTTTG GCGGCGCGCG GCGAAACCGT GGTCGAACGG ATTTACCACT TGGATCGCGG TTATGAAAAT ATTGAAAAAA AACTCGGCAG CGTCGGTGCG AACATCGAGC GCGTATCCGG CTGA

Details for NegoA.00952.a.B1.GE39980

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMV DKLKISANGP LNGEITVSGA KNAALPLMCA GLLTSGTLRL KNVPMLADVA TTQKLLQGMG ARVLTDNISE FEINGGTVNN TCAPYELVRT MRASILVLGP TLARFGEAQV SLPGGCAIGS RPVNQHLKGL EAMGAEIAIE HGYVKAKGKL KGARVAMDVV TVGGTENLLM AATLAEGTTV LENCAIEPEV VDLAECLVKM GAKISGIGTS TMIVEGAGEL YGCEHSVVPD RIEAGTFLCA VAITGGRVVL RNAAPKTMEV VLDKLVEAGA VIEAGDDWIA IDMRQRPKAV DIRTVVHPGF PTDMQAQFMA LNAVAEGSCR VVETIFENRF MHVPELNRMG ANITTEGNTA FVQGVERLSG AVVKATDLRA SASLVIAGLA ARGETVVERI YHLDRGYENI EKKLGS

Validated NT Sequence

acgctgccga gttttttttc aatattttca taaccgcgat ccaagtggta aatccgttcg accacggttt cgccgcgcgc cgccaaaccg gcgataacga ggctggcgga cgcgcgcaaa tccgtcgcct tgacgaccgc gccggaaagc cgttccacac cctgcacaaa tgccgtattg ccctcggttg taatgttcgc ccccatccgg ttcaactccg gaacgtgcat aaagcggttt tcaaaaatcg tttccaccac gcggcagctt ccctctgcca cggcattcaa tgccataaac tgcgcctgca tatccgtggg gaagccggga tggacgaccg tgcggatgtc caccgccttc ggacgctgcc gcatatcgat ggcgatccaa tcgtcgcccg cttcaatcac cgcacctgcc tcgaccagtt tgtccaacac cacttccatc gttttcgggg cggcattccg caaaaccacc ctgccgccgg ttatcgccac cgcacacagg aacgtccccg cttcaatccg gtcggggacg acgctgtgtt cgcagccgta cagctcgccc gcaccttcca caatcatggt cgatgttccg atgccgctga ttttcgcgcc cattttgacc aggcattccg ccaaatcgac cacttcaggc tcgatggcgc agttttccaa aaccgtcgta ccttccgcca gcgtcgccgc catcagcagg ttttccgtac cgccgacggt aacgacatcc atcgccacgc gcgcaccttt gagtttgcct ttggctttga cgtaaccgtg ttcgatggcg atttccgccc ccatcgcttc caagcctttc aaatgctgat tgacggggcg cgaaccgatg gcgcagccgc ccggcaggct gacttgcgcc tcgccgaaac gcgccagcgt cgggccaagc accaaaatcg aagcgcgcat cgtccgaacc aattcgtaag gggcgcaggt attgttgacc gtaccgccgt tgatttcaaa ttcgctgata ttgtcggtca gaacgcgcgc gcccatgccc tgaagcagct tttgcgtggt tgcgacatct gccagcatag ggacgttttt caggcgcaac gtacccgacg tcagcaaacc cgcgcacatc agcggcaatg ccgcgttttt cgcgcccgag accgttattt ccccgttgag cgggccgttt gcggagattt tcagtttgtc caccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMD KLKISANGPL NGEITVSGAK NAALPLMCAG LLTSGTLRLK NVPMLADVAT TQKLLQGMGA RVLTDNISEF EINGGTVNNT CAPYELVRTM RASILVLGPT LARFGEAQVS LPGGCAIGSR PVNQHLKGLE AMGAEIAIEH GYVKAKGKLK GARVAMDVVT VGGTENLLMA ATLAEGTTVL ENCAIEPEVV DLAECLVKMG AKISGIGTST MIVEGAGELY GCEHSVVPDR IEAGTFLCAV AITGGRVVLR NAAPKTMEVV LDKLVEAGAV IEAGDDWIAI DMRQRPKAVD IRTVVHPGFP TDMQAQFMAL NAVAEGSCRV VETIFENRFM HVPELNRMGA NITTEGNTAF VQGVERLSGA VVKATDLRAS ASLVIAGLAA RGETVVERIY HLDRGYENIE KKLGSVGANI ERVSG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggaca aactgaaaat ctccgcaaac ggcccgctca acggggaaat aacggtctcg ggcgcgaaaa acgcggcatt gccgctgatg tgcgcgggtt tgctgacatc gggtacgttg cgcctgaaaa acgtccctat gctggcagat gtcgcaacca cgcaaaagct gcttcagggc atgggcgcgc gcgttctgac cgacaatatc agcgaatttg aaatcaacgg cggtacggtc aacaatacct gcgcccctta cgaattggtt cggacgatgc gcgcttcgat tttggtgctt ggtccgacgc tggcgcgttt cggcgaggcg caagtcagcc tgccgggcgg ctgcgccatc ggttcgcgcc ccgtcaatca gcatttgaaa ggcttggaag cgatgggggc ggaaatcgcc atcgaacacg gttacgtcaa agccaaaggc aaactcaaag gtgcgcgcgt ggcgatggat gtcgttaccg tcggcggtac ggaaaacctg ctgatggcgg cgacgctggc ggaaggtacg acggttttgg aaaactgcgc catcgagcct gaagtggtcg atttggcgga atgcctggtc aaaatgggcg cgaaaatcag cggcatcgga acatcgacca tgattgtgga aggtgcgggc gagctgtacg gctgcgaaca cagcgtcgtc cccgaccgga ttgaagcggg gacgttcctg tgtgcggtgg cgataaccgg cggcagggtg gttttgcgga atgccgcccc gaaaacgatg gaagtggtgt tggacaaact ggtcgaggca ggtgcggtga ttgaagcggg cgacgattgg atcgccatcg atatgcggca gcgtccgaag gcggtggaca tccgcacggt cgtccatccc ggcttcccca cggatatgca ggcgcagttt atggcattga atgccgtggc ggaaggaagc tgccgcgtgg tggaaacgat ttttgaaaac cgctttatgc acgttccgga gttgaaccgg atgggggcga acattacaac cgagggcaat acggcatttg tgcagggtgt ggaacggctt tccggcgcgg tcgtcaaggc gacggatttg cgcgcgtccg ccagcctagt tatcgccggt ttggcggcgc gcggcgaaac cgtggtcgaa cggatttacc acttggatcg cggttatgaa aatattgaaa aaaaactcgg cagcgtcggt gcgaacatcg agcgcgtatc cggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for NegoA.00952.a.B1.PS38219

PURIFICATION DATe:	3/8/2017
CONCENTRATION:	46.1mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer: 20mM HEPES pH 7.0, 300mM NaCl, 5% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGACA AACTGAAAAT CTCCGCAAAC GGCCCGCTCA ACGGGGAAAT AACGGTCTCG GGCGCGAAAA ACGCGGCATT GCCGCTGATG TGCGCGGGTT TGCTGACATC GGGTACGTTG CGCCTGAAAA ACGTCCCTAT GCTGGCAGAT GTCGCAACCA CGCAAAAGCT GCTTCAGGGC ATGGGCGCGC GCGTTCTGAC CGACAATATC AGCGAATTTG AAATCAACGG CGGTACGGTC AACAATACCT GCGCCCCTTA CGAATTGGTT CGGACGATGC GCGCTTCGAT TTTGGTGCTT GGTCCGACGC TGGCGCGTTT CGGCGAGGCG CAAGTCAGCC TGCCGGGCGG CTGCGCCATC GGTTCGCGCC CCGTCAATCA GCATTTGAAA GGCTTGGAAG CGATGGGGGC GGAAATCGCC ATCGAACACG GTTACGTCAA AGCCAAAGGC AAACTCAAAG GTGCGCGCGT GGCGATGGAT GTCGTTACCG TCGGCGGTAC GGAAAACCTG CTGATGGCGG CGACGCTGGC GGAAGGTACG ACGGTTTTGG AAAACTGCGC CATCGAGCCT GAAGTGGTCG ATTTGGCGGA ATGCCTGGTC AAAATGGGCG CGAAAATCAG CGGCATCGGA ACATCGACCA TGATTGTGGA AGGTGCGGGC GAGCTGTACG GCTGCGAACA CAGCGTCGTC CCCGACCGGA TTGAAGCGGG GACGTTCCTG TGTGCGGTGG CGATAACCGG CGGCAGGGTG GTTTTGCGGA ATGCCGCCCC GAAAACGATG GAAGTGGTGT TGGACAAACT GGTCGAGGCA GGTGCGGTGA TTGAAGCGGG CGACGATTGG ATCGCCATCG ATATGCGGCA GCGTCCGAAG GCGGTGGACA TCCGCACGGT CGTCCATCCC GGCTTCCCCA CGGATATGCA GGCGCAGTTT ATGGCATTGA ATGCCGTGGC GGAAGGAAGC TGCCGCGTGG TGGAAACGAT TTTTGAAAAC CGCTTTATGC ACGTTCCGGA GTTGAACCGG ATGGGGGCGA ACATTACAAC CGAGGGCAAT ACGGCATTTG TGCAGGGTGT GGAACGGCTT TCCGGCGCGG TCGTCAAGGC GACGGATTTG CGCGCGTCCG CCAGCCTAGT TATCGCCGGT TTGGCGGCGC GCGGCGAAAC CGTGGTCGAA CGGATTTACC ACTTGGATCG CGGTTATGAA AATATTGAAA AAAAACTCGG CAGCGTCGGT GCGAACATCG AGCGCGTATC CGGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT