NegoA.00944.a
ribose-5-phosphate isomerase A
| CENTER ID: | NegoA.00944.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00944.a.B1.GE39978 | full length | 1 | 223 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00944.a.B1.PS37975 | full length | 1 | 223 |
Structures
5UF2
DEPOSITED: 1/3/2017
DETERMINATION: XRay
CLONE: NegoA.00944.a.B1.GE39978
PROTEIN: NegoA.00944.a.B1.PS37975
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.892 |
| UniProt: | B4RL16 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTQDELKRI AAEKAVEFVP ENEYIGIGTG STINFFIEAL GKSGKKIKGA VSTSKKSGEL LARYDIPVVS LNEVSGLAVY IDGADEVNHA LQMIKGGGGA HLNEKIVASA SEKFVCIADE SKYVSRLGKF PLPVEAVESA RSLVSRKLLA MGGQPELRIG YTTFYGNQIV DVHGLNIDQP LTMEDEINKI TGVLENGIFA RDAADVLILG TEEGAKVIYP CQG
NT Sequence
ATGACGACAC AAGACGAACT CAAGCGCATC GCCGCCGAAA AAGCAGTCGA ATTCGTACCC GAAAACGAAT ACATCGGCAT CGGTACCGGT TCGACCATCA ATTTCTTCAT CGAAGCCTTG GGCAAAAGCG GTAAAAAAAT CAAAGGCGCG GTTTCAACCT CCAAAAAATC CGGCGAACTG CTTGCCCGGT ACGACATTCC CGTCGTGTCC CTGAACGAAG TGTCCGGTTT GGCGGTCTAT ATCGACGGTG CGGACGAAGT GAACCACGCC CTGCAAATGA TTAAGGGCGG CGGCGGCGCG CACCTCAACG AAAAAATCGT CGCCAGCGCA TCAGAAAAAT TCGTCTGCAT TGCGGACGAA AGCAAATATG TTTCCCGGCT GGGCAAATTC CCACTGCCCG TGGAAGCCGT CGAAAGCGCG CGCTCCCTTG TTTCGCGCAA ACTGCTTGCC ATGGGCGGAC AGCCCGAACT GCGTATCGGC TACACCACGT TTTACGGCAA CCAAATCGTC GATGTCCACG GCTTGAATAT CGATCAGCCG CTGACGATGG AAGATGAAAT CAACAAAATC ACCGGCGTAC TCGAAAACGG CATATTCGCC CGCGATGCCG CAGATGTGTT GATTTTGGGG ACGGAAGAGG GTGCGAAAGT CATCTATCCC TGCCAAGGGT AA
Details for NegoA.00944.a.B1.GE39978
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT TQDELKRIAA EKAVEFVPEN EYIGIGTGST INFFIEALGK SGKKIKGAVS TSKKSGELLA RYDIPVVSLN EVSGLAVYID GADEVNHALQ MIKGGGGAHL NEKIVASASE KFVCIADESK YVSRLGKFPL PVEAVESARS LVSRKLLAMG GQPELRIGYT TFYGNQIVDV HGLNIDQPLT MEDEINKITG VLENGIFARD AADVLILGTE EGAKVIYPCQ G
Validated NT Sequence
atcttactca cttacccttg gcagggatag atgactttcg caccctcttc cgtccccaaa atcaacacat cggcggcatc gcgggcgaat atgccgtttt cgagtacgcc ggtgattttg ttgatttcat cttccatcgt cagcggctga tcgatattca agccgtggac atcgacgatt tggttgccgt aaaacgtggt gtagccgata cgcagttcgg gctgtccgcc catggcaagc agtttgcgcg aaacaaggga gcgcgcgctt tcgacggctt ccacgggcag tgggaatttg cccagccggg aaacatattt gctttcgtcc gcaatgcaga cgaatttttc tgatgcgctg gcgacgattt tttcgttgag gtgcgcgccg ccgccgccct taatcatttg cagggcgtgg ttcacttcgt ccgcaccgtc gatatagacc gccaaaccgg acacttcgtt cagggacacg acgggaatgt cgtaccgggc aagcagttcg ccggattttt tggaggttga aaccgcgcct ttgatttttt taccgctttt gcccaaggct tcgatgaaga aattgatggt cgaaccggta ccgatgccga tgtattcgtt ttcgggtacg aattcgactg ctttttcggc ggcgatgcgc ttgagttcgt cttgtgtcgt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT TQDELKRIAA EKAVEFVPEN EYIGIGTGST INFFIEALGK SGKKIKGAVS TSKKSGELLA RYDIPVVSLN EVSGLAVYID GADEVNHALQ MIKGGGGAHL NEKIVASASE KFVCIADESK YVSRLGKFPL PVEAVESARS LVSRKLLAMG GQPELRIGYT TFYGNQIVDV HGLNIDQPLT MEDEINKITG VLENGIFARD AADVLILGTE EGAKVIYPCQ G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cacaagacga actcaagcgc atcgccgccg aaaaagcagt cgaattcgta cccgaaaacg aatacatcgg catcggtacc ggttcgacca tcaatttctt catcgaagcc ttgggcaaaa gcggtaaaaa aatcaaaggc gcggtttcaa cctccaaaaa atccggcgaa ctgcttgccc ggtacgacat tcccgtcgtg tccctgaacg aagtgtccgg tttggcggtc tatatcgacg gtgcggacga agtgaaccac gccctgcaaa tgattaaggg cggcggcggc gcgcacctca acgaaaaaat cgtcgccagc gcatcagaaa aattcgtctg cattgcggac gaaagcaaat atgtttcccg gctgggcaaa ttcccactgc ccgtggaagc cgtcgaaagc gcgcgctccc ttgtttcgcg caaactgctt gccatgggcg gacagcccga actgcgtatc ggctacacca cgttttacgg caaccaaatc gtcgatgtcc acggcttgaa tatcgatcag ccgctgacga tggaagatga aatcaacaaa atcaccggcg tactcgaaaa cggcatattc gcccgcgatg ccgcagatgt gttgattttg gggacggaag agggtgcgaa agtcatctat ccctgccaag ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for NegoA.00944.a.B1.PS37975
| PURIFICATION DATe: | 7/12/2016 |
| CONCENTRATION: | 36.87mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT TQDELKRIAA EKAVEFVPEN EYIGIGTGST INFFIEALGK SGKKIKGAVS TSKKSGELLA RYDIPVVSLN EVSGLAVYID GADEVNHALQ MIKGGGGAHL NEKIVASASE KFVCIADESK YVSRLGKFPL PVEAVESARS LVSRKLLAMG GQPELRIGYT TFYGNQIVDV HGLNIDQPLT MEDEINKITG VLENGIFARD AADVLILGTE EGAKVIYPCQ G
Validated NT Sequence
atcttactca cttacccttg gcagggatag atgactttcg caccctcttc cgtccccaaa atcaacacat cggcggcatc gcgggcgaat atgccgtttt cgagtacgcc ggtgattttg ttgatttcat cttccatcgt cagcggctga tcgatattca agccgtggac atcgacgatt tggttgccgt aaaacgtggt gtagccgata cgcagttcgg gctgtccgcc catggcaagc agtttgcgcg aaacaaggga gcgcgcgctt tcgacggctt ccacgggcag tgggaatttg cccagccggg aaacatattt gctttcgtcc gcaatgcaga cgaatttttc tgatgcgctg gcgacgattt tttcgttgag gtgcgcgccg ccgccgccct taatcatttg cagggcgtgg ttcacttcgt ccgcaccgtc gatatagacc gccaaaccgg acacttcgtt cagggacacg acgggaatgt cgtaccgggc aagcagttcg ccggattttt tggaggttga aaccgcgcct ttgatttttt taccgctttt gcccaaggct tcgatgaaga aattgatggt cgaaccggta ccgatgccga tgtattcgtt ttcgggtacg aattcgactg ctttttcggc ggcgatgcgc ttgagttcgt cttgtgtcgt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMT TQDELKRIAA EKAVEFVPEN EYIGIGTGST INFFIEALGK SGKKIKGAVS TSKKSGELLA RYDIPVVSLN EVSGLAVYID GADEVNHALQ MIKGGGGAHL NEKIVASASE KFVCIADESK YVSRLGKFPL PVEAVESARS LVSRKLLAMG GQPELRIGYT TFYGNQIVDV HGLNIDQPLT MEDEINKITG VLENGIFARD AADVLILGTE EGAKVIYPCQ G
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA CACAAGACGA ACTCAAGCGC ATCGCCGCCG AAAAAGCAGT CGAATTCGTA CCCGAAAACG AATACATCGG CATCGGTACC GGTTCGACCA TCAATTTCTT CATCGAAGCC TTGGGCAAAA GCGGTAAAAA AATCAAAGGC GCGGTTTCAA CCTCCAAAAA ATCCGGCGAA CTGCTTGCCC GGTACGACAT TCCCGTCGTG TCCCTGAACG AAGTGTCCGG TTTGGCGGTC TATATCGACG GTGCGGACGA AGTGAACCAC GCCCTGCAAA TGATTAAGGG CGGCGGCGGC GCGCACCTCA ACGAAAAAAT CGTCGCCAGC GCATCAGAAA AATTCGTCTG CATTGCGGAC GAAAGCAAAT ATGTTTCCCG GCTGGGCAAA TTCCCACTGC CCGTGGAAGC CGTCGAAAGC GCGCGCTCCC TTGTTTCGCG CAAACTGCTT GCCATGGGCG GACAGCCCGA ACTGCGTATC GGCTACACCA CGTTTTACGG CAACCAAATC GTCGATGTCC ACGGCTTGAA TATCGATCAG CCGCTGACGA TGGAAGATGA AATCAACAAA ATCACCGGCG TACTCGAAAA CGGCATATTC GCCCGCGATG CCGCAGATGT GTTGATTTTG GGGACGGAAG AGGGTGCGAA AGTCATCTAT CCCTGCCAAG GGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT