NegoA.00865.a
phosphoribosylamine-glycine ligase
| CENTER ID: | NegoA.00865.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00865.a.B1.GE39977 | full length | 1 | 423 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5VEV
DEPOSITED: 4/5/2017
DETERMINATION: XRay
CLONE: NegoA.00865.a.B1.GE39977
PROTEIN: NegoA.00865.a.B1.PW37919
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.2485 |
| UniProt: | B4RPN9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLLVIGNGG REHALAWKLA QSPKVETVFV APGNAGTAIE SKLQNIALTA YQDLIEFCRK ENIVFTVVGP EAPLAAGIVD DFRAAGLKIF GPTQYAAQLE SSKDFAKAFM VKYNIPTAQY QTFENADAAH DYVNQKGAPI VIKADGLVAG KGVIVAMTLD EAHAAIDDML LGNKMGNAGE RVVIEDFLQG EEASFIVMVD GNHVLPMATS RDHKRLLDGD KGPNTGGMGA YSPAPVVTPA VYERAMNEII LPTVAGMKAE GHEFTGFLYA GLMIDQSGAP YTIEFNCRFG DPETQPIMSR LNSDLADLVE AAIDGRLDSV KAEWNPQTAV GVVLAAQNYP ETPKKGDVIS GLDDVNRIGK VFHAGTTVNE KGDVLTNGGR ILCVVGLGDD VAQAKAKAYG ALEKISFDGM QYRKDIADKA INR
NT Sequence
ATGAAACTGC TGGTTATCGG TAATGGCGGT CGCGAACACG CGCTGGCTTG GAAGTTGGCG CAGTCGCCCA AAGTGGAAAC AGTATTTGTT GCGCCCGGTA ATGCCGGTAC GGCGATTGAA TCCAAGCTCC AAAACATTGC CTTGACCGCG TATCAGGATT TGATTGAATT CTGCCGTAAA GAAAATATTG TTTTTACCGT CGTCGGCCCT GAAGCGCCTT TGGCGGCAGG TATTGTGGAT GATTTTCGTG CCGCAGGGCT GAAAATATTT GGTCCGACAC AATATGCGGC GCAGTTGGAA AGTTCTAAAG ATTTCGCCAA AGCATTTATG GTGAAATACA ATATTCCGAC CGCGCAATAC CAAACCTTTG AAAATGCCGA TGCCGCACAT GATTACGTCA ATCAGAAAGG TGCGCCTATC GTTATCAAAG CCGATGGTTT GGTGGCAGGT AAAGGCGTGA TTGTGGCAAT GACTTTGGAT GAAGCGCATG CTGCGATTGA CGATATGTTG TTGGGCAATA AAATGGGCAA TGCCGGCGAA CGCGTTGTGA TTGAAGATTT TCTGCAAGGC GAAGAAGCGA GCTTTATCGT TATGGTTGAT GGCAATCATG TATTACCTAT GGCGACCAGT CGAGACCACA AACGTCTTTT AGACGGAGAT AAAGGCCCGA ATACGGGCGG TATGGGTGCG TACAGTCCCG CGCCTGTGGT AACGCCTGCT GTGTACGAGC GTGCGATGAA CGAGATTATT TTGCCGACCG TAGCGGGTAT GAAAGCGGAG GGGCACGAGT TCACCGGCTT TTTGTACGCA GGTTTGATGA TCGATCAAAG CGGTGCACCC TATACGATTG AGTTTAACTG CCGTTTCGGC GATCCCGAAA CCCAGCCGAT TATGAGCCGT CTAAACAGCG ATTTGGCGGA TTTGGTTGAA GCGGCAATAG ACGGCAGACT GGACAGCGTC AAAGCGGAAT GGAATCCGCA AACTGCCGTG GGTGTGGTAC TGGCGGCGCA AAATTACCCC GAAACACCCA AGAAAGGCGA TGTTATTTCC GGTTTGGATG ACGTCAACCG AATCGGCAAA GTATTCCATG CCGGCACAAC GGTAAACGAG AAAGGCGACG TGTTGACCAA CGGCGGGCGC ATATTGTGTG TTGTAGGATT GGGCGACGAT GTGGCGCAAG CGAAAGCCAA GGCGTATGGC GCGTTGGAAA AAATCAGCTT CGACGGGATG CAATACCGTA AAGACATTGC CGATAAAGCG ATTAACCGTT AA
Details for NegoA.00865.a.B1.GE39977
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMK LLVIGNGGRE HALAWKLAQS PKVETVFVAP GNAGTAIESK LQNIALTAYQ DLIEFCRKEN IVFTVVGPEA PLAAGIVDDF RAAGLKIFGP TQYAAQLESS KDFAKAFMVK YNIPTAQYQT FENADAAHDY VNQKGAPIVI KADGLVAGKG VIVAMTLDEA HAAIDDMLLG NKMGNAGERV VIEDFLQGEE ASFIVMVDGN HVLPMATSQD HKRLLDGDKG PNTGGMGAYS PAPVVTPAVY ERAMNEIILP TVAGMKAEGH EFTGFLYAGL MIDQSGAPYT IEFNCRFGDP ETQPIMSRLN SDLADLVEAA IDGRLDSVKA EWNPQTAVGV VLAAQNYPET PKKGDVISGL DDVNRIGKVF HAGTTVNEKG DVLTNGGRIL CVVGLGDDVA QAKAKAYGAL EKISFDGMQY RK
Validated NT Sequence
ctttacggta ttgcatcccg tcgaagctga ttttttccaa cgcgccatac gccttggctt tcgcttgcgc cacatcgtcg cccaatccta caacacacaa tatgcgcccg ccgttggtca acacgtcgcc tttctcgttt accgttgtgc cggcatggaa tactttgccg attcggttga cgtcatccaa accggaaata acatcgcctt tcttgggtgt ttcggggtaa ttttgcgccg ccagtaccac acccacggca gtttgcggat tccattccgc tttgacgctg tccagtctgc cgtctattgc cgcttcaacc aaatccgcca aatcgctgtt tagacggctc ataatcggct gggtttcggg atcgccgaaa cggcagttaa actcaatcgt atagggtgca ccgctttgat cgatcatcaa acctgcgtac aaaaagccgg taaactcgtg cccctccgct ttcatacccg ctacggtcgg caaaataatc tcgttcatcg cacgctcgta cacagcaggc gttaccacag gcgcgggact gtacgcaccc ataccgcccg tattcgggcc tttatctccg tctaaaagac gtttgtggtc ttgactggtc gccataggca atacatgatt gccatcaacc ataacgataa agctcgcttc ttcgccttgc agaaaatctt caatcacaac gcgttcgccg gcattgccca ttttattgcc caacaacata tcgtcaatcg cagcatgcgc ttcatccaaa gtcattgcca caatcacgcc tttacctgcc accaaaccat cggctttgat aacgataggc gcacctttct gattgacgta atcatgtgcg gcatcggcat tttcaaaggt ttggtattgc gcggtcggaa tattgtattt caccataaat gctttggcga aatctttaga actttccaac tgcgccgcat attgtgtcgg accaaatatt ttcagccctg cggcacgaaa atcatccaca atacctgccg ccaaaggcgc ttcagggccg acgacggtaa aaacaatatt ttctttacgg cagaattcaa tcaaatcctg atacgcggtc aaggcaatgt tttggagctt ggattcaatc gccgtaccgg cattaccggg cgcaacaaat actgtttcca ctttgggcga ctgcgccaac ttccaagcca gcgcgtgttc gcgaccgcca ttaccgataa ccagcagttt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMK LLVIGNGGRE HALAWKLAQS PKVETVFVAP GNAGTAIESK LQNIALTAYQ DLIEFCRKEN IVFTVVGPEA PLAAGIVDDF RAAGLKIFGP TQYAAQLESS KDFAKAFMVK YNIPTAQYQT FENADAAHDY VNQKGAPIVI KADGLVAGKG VIVAMTLDEA HAAIDDMLLG NKMGNAGERV VIEDFLQGEE ASFIVMVDGN HVLPMATSRD HKRLLDGDKG PNTGGMGAYS PAPVVTPAVY ERAMNEIILP TVAGMKAEGH EFTGFLYAGL MIDQSGAPYT IEFNCRFGDP ETQPIMSRLN SDLADLVEAA IDGRLDSVKA EWNPQTAVGV VLAAQNYPET PKKGDVISGL DDVNRIGKVF HAGTTVNEKG DVLTNGGRIL CVVGLGDDVA QAKAKAYGAL EKISFDGMQY RKDIADKAIN R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac tgctggttat cggtaatggc ggtcgcgaac acgcgctggc ttggaagttg gcgcagtcgc ccaaagtgga aacagtattt gttgcgcccg gtaatgccgg tacggcgatt gaatccaagc tccaaaacat tgccttgacc gcgtatcagg atttgattga attctgccgt aaagaaaata ttgtttttac cgtcgtcggc cctgaagcgc ctttggcggc aggtattgtg gatgattttc gtgccgcagg gctgaaaata tttggtccga cacaatatgc ggcgcagttg gaaagttcta aagatttcgc caaagcattt atggtgaaat acaatattcc gaccgcgcaa taccaaacct ttgaaaatgc cgatgccgca catgattacg tcaatcagaa aggtgcgcct atcgttatca aagccgatgg tttggtggca ggtaaaggcg tgattgtggc aatgactttg gatgaagcgc atgctgcgat tgacgatatg ttgttgggca ataaaatggg caatgccggc gaacgcgttg tgattgaaga ttttctgcaa ggcgaagaag cgagctttat cgttatggtt gatggcaatc atgtattacc tatggcgacc agtcgagacc acaaacgtct tttagacgga gataaaggcc cgaatacggg cggtatgggt gcgtacagtc ccgcgcctgt ggtaacgcct gctgtgtacg agcgtgcgat gaacgagatt attttgccga ccgtagcggg tatgaaagcg gaggggcacg agttcaccgg ctttttgtac gcaggtttga tgatcgatca aagcggtgca ccctatacga ttgagtttaa ctgccgtttc ggcgatcccg aaacccagcc gattatgagc cgtctaaaca gcgatttggc ggatttggtt gaagcggcaa tagacggcag actggacagc gtcaaagcgg aatggaatcc gcaaactgcc gtgggtgtgg tactggcggc gcaaaattac cccgaaacac ccaagaaagg cgatgttatt tccggtttgg atgacgtcaa ccgaatcggc aaagtattcc atgccggcac aacggtaaac gagaaaggcg acgtgttgac caacggcggg cgcatattgt gtgttgtagg attgggcgac gatgtggcgc aagcgaaagc caaggcgtat ggcgcgttgg aaaaaatcag cttcgacggg atgcaatacc gtaaagacat tgccgataaa gcgattaacc gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat