NegoA.00852.a
GTP-binding protein EngA
| CENTER ID: | NegoA.00852.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00852.a.AE7.GE44063 | truncation #3 | 1 | 457 | ||
| NegoA.00852.a.B4.GE43175 | robetta construct 1 | 15 | 450 | ||
| NegoA.00852.a.B5.GE43176 | robetta construct 2 | 18 | 448 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00852.a.D11.PD38388 | full length | 1 | 457 | ||
| NegoA.00852.a.TH11.PD38460 | full length | 183 | 457 | ||
| NegoA.00852.a.AE7.PW38937 | truncation #3 | 1 | 457 |
Structures
6XRS
DEPOSITED: 7/13/2020
DETERMINATION: XRay
CLONE: NegoA.00852.a.D11.GE43396
PROTEIN: NegoA.00852.a.D11.PD38388
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_85040 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.640 |
| UniProt: | B4RKD2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSSTERHLT QKDPIMKPTI ALIGRPNVGK STLFNRLTRT KDALVHDLPG LTRDRHYGHG KVGSKPYFVI DTGGFEPVVD SGILHEMAKQ TLQAVDEADA VVFLVDGRTG LTPQDKIIAD RLRQSPRPVY LAVNKGEGGD RAVLAAEFYE LALGEPHVIS GAHGDGVYYL IEEILENFPE PEAEEADAKH PVFAVIGRPN VGKSTLVNAI LGEKRVIAFD MAGTTRDSIH IDFEREGKPF TIIDTAGVRR RGKVDEAVEK FSVIKAMQAV EAANVAVLVL DAQQDIADQD ATIAGFALEA GRALVVAVNK WDGISEERRE QVKRDISRKL YFLDFAKFHF ISALKERGID GLFESIQAAY NAAMIKMPTP KITRVLQTAV GRQQPPRAGL VRPKMRYAHQ GGMNPPVIVV HGNSLHAISD SYTRYLTQTF RKAFNLQGTP LRIQYNVSEN PYENAEDKPK KKPLRRVSLS NRIEKREGRK EEKNRFKKKT KVSVKKQFSK
NT Sequence
GTGTTCAGTT CTACCGAACG GCACTTGACA CAGAAAGACC CAATCATGAA ACCAACCATC GCGCTTATCG GCCGCCCCAA CGTCGGCAAA TCTACCTTGT TCAACCGTTT GACGCGCACC AAAGACGCGC TCGTGCATGA CCTGCCGGGT CTGACCCGCG ACCGCCATTA CGGACACGGC AAAGTCGGCA GCAAACCTTA TTTTGTCATC GATACCGGCG GTTTCGAGCC GGTTGTGGAC AGCGGCATTT TGCACGAAAT GGCAAAACAA ACCTTGCAGG CTGTCGATGA AGCCGATGCG GTTGTGTTTT TGGTGGACGG CCGTACCGGT TTAACACCGC AAGACAAGAT TATTGCCGAC CGTTTGCGCC AAAGTCCGCG TCCCGTTTAT TTGGCCGTGA ATAAAGGCGA GGGCGGCGAC AGGGCCGTAC TTGCCGCCGA GTTTTACGAG CTGGCATTGG GCGAGCCGCA CGTTATTTCC GGCGCGCACG GCGACGGCGT GTATTACCTG ATTGAAGAAA TTTTAGAAAA CTTCCCCGAG CCTGAAGCCG AAGAAGCCGA TGCAAAACAT CCCGTTTTTG CCGTTATCGG TCGTCCGAAC GTCGGCAAAT CTACGCTGGT TAACGCCATT CTCGGCGAAA AACGCGTCAT CGCCTTCGAT ATGGCAGGCA CAACGCGCGA CAGCATCCAC ATCGATTTCG AGCGCGAAGG CAAACCGTTT ACCATCATCG ATACCGCAGG CGTGCGCCGT CGCGGCAAAG TGGATGAAGC GGTGGAAAAG TTCTCCGTTA TCAAAGCTAT GCAGGCGGTT GAAGCGGCAA ACGTTGCTGT TTTGGTATTG GACGCGCAAC AGGACATTGC CGACCAAGAT GCGACGATTG CCGGTTTTGC TTTGGAAGCG GGACGCGCGC TGGTAGTTGC CGTCAACAAA TGGGACGGCA TCAGCGAAGA GCGGCGCGAA CAAGTGAAAC GCGACATTTC CCGCAAACTG TATTTCCTCG ATTTTGCCAA GTTCCACTTT ATTTCCGCGT TGAAAGAGCG CGGTATAGAC GGTTTGTTTG AAAGCATTCA AGCTGCCTAC AACGCGGCGA TGATTAAGAT GCCGACGCCG AAAATCACGC GCGTATTGCA AACCGCCGTC GGACGCCAAC AGCCGCCGCG TGCCGGCTTG GTGCGTCCGA AAATGCGTTA TGCCCACCAA GGCGGCATGA ATCCGCCGGT GATTGTGGTA CACGGCAATT CGCTGCACGC GATTTCCGAC AGCTATACGC GCTATCTGAC CCAGACCTTC CGCAAAGCCT TCAACCTGCA AGGCACGCCG TTGCGGATTC AATACAATGT TTCGGAAAAC CCGTATGAAA ATGCGGAAGA CAAACCGAAG AAAAAACCGC TGCGCCGCGT CAGCCTGAGC AACCGTATTG AGAAACGCGA AGGCCGAAAG GAAGAGAAAA ACCGCTTCAA GAAGAAAACC AAAGTCAGTG TGAAAAAACA ATTCAGCAAA TAA
Details for NegoA.00852.a.AE7.GE44063
| HARVESTED ON: | 1/7/2021 |
| SEQUENCED ON: | 1/8/2021 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MVFSSTERHL TQKDPIMKPT IALIGRPNVG KSTLFNRLTR TKDALVHDLP GLTRDRHYGH GKVGSKPYFV IDTGGFEPVV DSGILHEMAK QTLQAVDEAD AVVFLVDGRT GLTPQDKIIA DRLRQSPRPV YLAVNKGEGG DRAVLAAEFY ELALGEPHVI SGAHGDGVYY LIEEILENFP EPEAEEADAK HPVFAVIGRP NVGKSTLVNA ILGEKRVIAF DMAGTTRDSI HIDFEREGKP FTIIDTAGVR RRGKVDEAVE KFSVIKAMQA VEAANVAVLV LDAQQDIADQ DATIAGFALE AGRALVVAVN KWDGISEERR EQVKRDISRK LYFLDFAKFH FISALKERGI DGLFESIQAA YNAAMIKMPT PKITRVLQTA VERQQPPRAG LVRPKMRYAH QGGMNPPVIV VHGNSLHAIS DSYTRYLTQT FRKAFNLQGT PLRIQYNVSE NPYENAEDAG HHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgtcttccgc attttcatac gggttttccg aaacattgta ttgaatccgc aacggcgtgc cttgcaggtt gaaggctttg cggaaggtct gggtcagata gcgcgtatag ctgtcggaaa tcgcgtgcag cgaattgccg tgaaccacaa tcaccggcgg attcatgccg ccttggtggg cataacgcat tttcggacgc accaagccgg cacgcggcgg ctgttggcgt tcgacggcgg tttgcaatac gcgcgtgatt ttcggcgtcg gcatcttaat catcgccgcg ttgtaggcag cttgaatgct ttcaaacaaa ccgtctatac cgcgctcttt caacgcggaa ataaagtgga acttggcaaa atcgaggaaa tacagtttgc gggaaatgtc gcgtttcact tgttcgcgcc gctcttcgct gatgccgtcc catttgttga cggcaactac cagcgcgcgt cccgcttcca aagcaaaacc ggcaatcgtc gcatcttggt cggcaatgtc ctgttgcgcg tccaatacca aaacagcaac gtttgccgct tcaaccgcct gcatggcttt gataacggag aacttttcca ccgcttcatc cactttgccg cgacggcgca cgcctgcggt atcgatgatg gtaaacggtt tgccttcgcg ctcgaaatcg atgtggatgc tgtcgcgcgt tgtgcctgcc atatcgaagg cgatgacgcg tttttcgccg agaatggcgt taaccagcgt agatttgccg acgttcggac gaccgataac ggcaaaaacg ggatgttttg catcggcttc ttcggcttca ggctcgggga agttttctaa aatttcttca atcaggtaat acacgccgtc gccgtgcgcg ccggaaataa cgtgcggctc gcccaatgcc agctcgtaaa actcggcggc aagtacggcc ctgtcgccgc cctcgccttt attcacggcc aaataaacgg gacgcggact ttggcgcaaa cggtcggcaa taatcttgtc ttgcggtgtt aaaccggtac ggccgtccac caaaaacaca accgcatcgg cttcatcgac agcctgcaag gtttgttttg ccatttcgtg caaaatgccg ctgtccacaa ccggctcgaa accgccggta tcgatgacaa aataaggttt gctgccgact ttgccgtgtc cgtaatggcg gtcgcgggtc agacccggca ggtcatgcac gagcgcgtct ttggtgcgcg tcaaacggtt gaacaaggta gatttgccga cgttggggcg gccgataagc gcgatggttg gtttcatgat tgggtctttc tgtgtcaagt gccgttcggt agaactgaac accatgggag aga
Expected Protein Sequence
MFSSTERHLT QKDPIMKPTI ALIGRPNVGK STLFNRLTRT KDALVHDLPG LTRDRHYGHG KVGSKPYFVI DTGGFEPVVD SGILHEMAKQ TLQAVDEADA VVFLVDGRTG LTPQDKIIAD RLRQSPRPVY LAVNKGEGGD RAVLAAEFYE LALGEPHVIS GAHGDGVYYL IEEILENFPE PEAEEADAKH PVFAVIGRPN VGKSTLVNAI LGEKRVIAFD MAGTTRDSIH IDFEREGKPF TIIDTAGVRR RGKVDEAVEK FSVIKAMQAV EAANVAVLVL DAQQDIADQD ATIAGFALEA GRALVVAVNK WDGISEERRE QVKRDISRKL YFLDFAKFHF ISALKERGID GLFESIQAAY NAAMIKMPTP KITRVLQTAV GRQQPPRAGL VRPKMRYAHQ GGMNPPVIVV HGNSLHAISD SYTRYLTQTF RKAFNLQGTP LRIQYNVSEN PYENAEDGHH HHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gttcagttct accgaacggc acttgacaca gaaagaccca atcatgaaac caaccatcgc gcttatcggc cgccccaacg tcggcaaatc taccttgttc aaccgtttga cgcgcaccaa agacgcgctc gtgcatgacc tgccgggtct gacccgcgac cgccattacg gacacggcaa agtcggcagc aaaccttatt ttgtcatcga taccggcggt ttcgagccgg ttgtggacag cggcattttg cacgaaatgg caaaacaaac cttgcaggct gtcgatgaag ccgatgcggt tgtgtttttg gtggacggcc gtaccggttt aacaccgcaa gacaagatta ttgccgaccg tttgcgccaa agtccgcgtc ccgtttattt ggccgtgaat aaaggcgagg gcggcgacag ggccgtactt gccgccgagt tttacgagct ggcattgggc gagccgcacg ttatttccgg cgcgcacggc gacggcgtgt attacctgat tgaagaaatt ttagaaaact tccccgagcc tgaagccgaa gaagccgatg caaaacatcc cgtttttgcc gttatcggtc gtccgaacgt cggcaaatct acgctggtta acgccattct cggcgaaaaa cgcgtcatcg ccttcgatat ggcaggcaca acgcgcgaca gcatccacat cgatttcgag cgcgaaggca aaccgtttac catcatcgat accgcaggcg tgcgccgtcg cggcaaagtg gatgaagcgg tggaaaagtt ctccgttatc aaagctatgc aggcggttga agcggcaaac gttgctgttt tggtattgga cgcgcaacag gacattgccg accaagatgc gacgattgcc ggttttgctt tggaagcggg acgcgcgctg gtagttgccg tcaacaaatg ggacggcatc agcgaagagc ggcgcgaaca agtgaaacgc gacatttccc gcaaactgta tttcctcgat tttgccaagt tccactttat ttccgcgttg aaagagcgcg gtatagacgg tttgtttgaa agcattcaag ctgcctacaa cgcggcgatg attaagatgc cgacgccgaa aatcacgcgc gtattgcaaa ccgccgtcgg acgccaacag ccgccgcgtg ccggcttggt gcgtccgaaa atgcgttatg cccaccaagg cggcatgaat ccgccggtga ttgtggtaca cggcaattcg ctgcacgcga tttccgacag ctatacgcgc tatctgaccc agaccttccg caaagccttc aacctgcaag gcacgccgtt gcggattcaa tacaatgttt cggaaaaccc gtatgaaaat gcggaagacg ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
Details for NegoA.00852.a.B4.GE43175
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/24/2019 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHIM KPTIALIGRP NVGKSTLFNR LTRTKDALVH DLPGLTRDRH YGHGKVGSKP YFVIDTGGFE PVVDSGILHE MAKQTLQAVD EADAVVFLVD GRTGLTPQDK IIADRLRQSP RPVYLAVNKG EGGDRAVLAA EFYELALGEP HVISGAHGDG VYYLIEEILE NFPEPEAEEA DAKHPVFAVI GRPNVGKSTL VNAILGEKRV IAFDMAGTTR DSIHIDFERE GKPFTIIDTA GVRRRGKVDE AVEKFSVIKA MQAVEAANVA VLVLDAQQDI ADQDATIAGF ALEAGRALVV AVNKWDGISE ERREQVKRDI SRKLYFLDFA KFHFISALKE RGIDGLFESI QAAYNAAMIK MPTPKITRVL QTAVGRQQPP RAGLVRPKMR YAHQGGMNPP VIVVHGNSLH AISDSYTRYL TQTFRKAFNL QGTPLRIQYN VSEN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcatga aaccaaccat cgcgcttatc ggccgcccca acgtcggcaa atctaccttg ttcaaccgtt tgacgcgcac caaagacgcg ctcgtgcatg acctgccggg tctgacccgc gaccgccatt acggacacgg caaagtcggc agcaaacctt attttgtcat cgataccggc ggtttcgagc cggttgtgga cagcggcatt ttgcacgaaa tggcaaaaca aaccttgcag gctgtcgatg aagccgatgc ggttgtgttt ttggtggacg gccgtaccgg tttaacaccg caagacaaga ttattgccga ccgtttgcgc caaagtccgc gtcccgttta tttggccgtg aataaaggcg agggcggcga cagggccgta cttgccgccg agttttacga gctggcattg ggcgagccgc acgttatttc cggcgcgcac ggcgacggcg tgtattacct gattgaagaa attttagaaa acttccccga gcctgaagcc gaagaagccg atgcaaaaca tcccgttttt gccgttatcg gtcgtccgaa cgtcggcaaa tctacgctgg ttaacgccat tctcggcgaa aaacgcgtca tcgccttcga tatggcaggc acaacgcgcg acagcatcca catcgatttc gagcgcgaag gcaaaccgtt taccatcatc gataccgcag gcgtgcgccg tcgcggcaaa gtggatgaag cggtggaaaa gttctccgtt atcaaagcta tgcaggcggt tgaagcggca aacgttgctg ttttggtatt ggacgcgcaa caggacattg ccgaccaaga tgcgacgatt gccggttttg ctttggaagc gggacgcgcg ctggtagttg ccgtcaacaa atgggacggc atcagcgaag agcggcgcga acaagtgaaa cgcgacattt cccgcaaact gtatttcctc gattttgcca agttccactt tatttccgcg ttgaaagagc gcggtataga cggtttgttt gaaagcattc aagctgccta caacgcggcg atgattaaga tgccgacgcc gaaaatcacg cgcgtattgc aaaccgccgt cggacgccaa cagccgccgc gtgccggctt ggtgcgtccg aaaatgcgtt atgcccacca aggcggcatg aatccgccgg tgattgtggt acacggcaat tcgctgcacg cgatttccga cagctatacg cgctatctga cccagacctt ccgcaaagcc ttcaacctgc aaggcacgcc gttgcggatt caatacaatg tttcggaaaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NegoA.00852.a.B5.GE43176
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/24/2019 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHPT IALIGRPNVG KSTLFNRLTR TKDALVHDLP GLTRDRHYGH GKVGSKPYFV IDTGGFEPVV DSGILHEMAK QTLQAVDEAD AVVFLVDGRT GLTPQDKIIA DRLRQSPRPV YLAVNKGEGG DRAVLAAEFY ELALGEPHVI SGAHGDGVYY LIEEILENFP EPEAEEADAK HPVFAVIGRP NVGKSTLVNA ILGEKRVIAF DMAGTTRDSI HIDFEREGKP FTIIDTAGVR RRGKVDEAVE KFSVIKAMQA VEAANVAVLV LDAQQDIADQ DATIAGFALE AGRALVVAVN KWDGISEERR EQVKRDISRK LYFLDFAKFH FISALKERGI DGLFESIQAA YNAAMIKMPT PKITRVLQTA VGRQQPPRAG LVRPKMRYAH QGGMNPPVIV VHGNSLHAIS DSYTRYLTQT FRKAFNLQGT PLRIQYNVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccaacca tcgcgcttat cggccgcccc aacgtcggca aatctacctt gttcaaccgt ttgacgcgca ccaaagacgc gctcgtgcat gacctgccgg gtctgacccg cgaccgccat tacggacacg gcaaagtcgg cagcaaacct tattttgtca tcgataccgg cggtttcgag ccggttgtgg acagcggcat tttgcacgaa atggcaaaac aaaccttgca ggctgtcgat gaagccgatg cggttgtgtt tttggtggac ggccgtaccg gtttaacacc gcaagacaag attattgccg accgtttgcg ccaaagtccg cgtcccgttt atttggccgt gaataaaggc gagggcggcg acagggccgt acttgccgcc gagttttacg agctggcatt gggcgagccg cacgttattt ccggcgcgca cggcgacggc gtgtattacc tgattgaaga aattttagaa aacttccccg agcctgaagc cgaagaagcc gatgcaaaac atcccgtttt tgccgttatc ggtcgtccga acgtcggcaa atctacgctg gttaacgcca ttctcggcga aaaacgcgtc atcgccttcg atatggcagg cacaacgcgc gacagcatcc acatcgattt cgagcgcgaa ggcaaaccgt ttaccatcat cgataccgca ggcgtgcgcc gtcgcggcaa agtggatgaa gcggtggaaa agttctccgt tatcaaagct atgcaggcgg ttgaagcggc aaacgttgct gttttggtat tggacgcgca acaggacatt gccgaccaag atgcgacgat tgccggtttt gctttggaag cgggacgcgc gctggtagtt gccgtcaaca aatgggacgg catcagcgaa gagcggcgcg aacaagtgaa acgcgacatt tcccgcaaac tgtatttcct cgattttgcc aagttccact ttatttccgc gttgaaagag cgcggtatag acggtttgtt tgaaagcatt caagctgcct acaacgcggc gatgattaag atgccgacgc cgaaaatcac gcgcgtattg caaaccgccg tcggacgcca acagccgccg cgtgccggct tggtgcgtcc gaaaatgcgt tatgcccacc aaggcggcat gaatccgccg gtgattgtgg tacacggcaa ttcgctgcac gcgatttccg acagctatac gcgctatctg acccagacct tccgcaaagc cttcaacctg caaggcacgc cgttgcggat tcaatacaat gtttcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NegoA.00852.a.D11.PD38388
| PURIFICATION DATe: | 8/25/2019 |
| CONCENTRATION: | 3.75mg/ml |
| OBSERVED MW: | 62kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | PUR1978, CID102204, PID7832-1 |
| EXPRESSION HOST: | BL21 DE3 |
| VIAL COUNT (approx.): | 24 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMFS STERHLTQKD PIMKPTIALI GRPNVGKSTL FNRLTRTKDA LVHDLPGLTR DRHYGHGKVG SKPYFVIDTG GFEPVVDSGI LHEMAKQTLQ AVDEADAVVF LVDGRTGLTP QDKIIADRLR QSPRPVYLAV NKGEGGDRAV LAAEFYELAL GEPHVISGAH GDGVYYLIEE ILENFPEPEA EEADAKHPVF AVIGRPNVGK STLVNAILGE KRVIAFDMAG TTRDSIHIDF EREGKPFTII DTAGVRRRGK VDEAVEKFSV IKAMQAVEAA NVAVLVLDAQ QDIADQDATI AGFALEAGRA LVVAVNKWDG ISEERREQVK RDISRKLYFL DFAKFHFISA LKERGIDGLF ESIQAAYNAA MIKMPTPKIT RVLQTAVGRQ QPPRAGLVRP KMRYAHQGGM NPPVIVVHGN SLHAISDSYT RYLTQTFRKA FNLQGTPLRI QYNVSENPYE NAED
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGTTCAGC AGCACCGAGC GTCACCTGAC CCAGAAGGAC CCGATCATGA AACCGACCAT CGCGCTGATT GGTCGTCCGA ACGTTGGCAA GAGCACCCTG TTTAACCGTC TGACCCGTAC CAAAGACGCG CTGGTGCACG ATCTGCCGGG TCTGACCCGT GATCGTCACT ACGGTCACGG CAAGGTTGGC AGCAAACCGT ATTTCGTGAT CGACACCGGT GGCTTTGAGC CGGTGGTTGA TAGCGGTATT CTGCACGAAA TGGCGAAGCA GACCCTGCAA GCGGTTGACG AAGCGGATGC GGTGGTTTTC CTGGTGGATG GTCGTACCGG TCTGACCCCG CAGGACAAGA TCATTGCGGA TCGTCTGCGT CAAAGCCCGC GTCCGGTTTA CCTGGCGGTG AACAAAGGTG AGGGTGGCGA TCGTGCGGTT CTGGCGGCGG AGTTTTATGA ACTGGCGCTG GGTGAACCGC ACGTTATCAG CGGCGCGCAC GGTGACGGCG TGTACTATCT GATCGAGGAA ATTCTGGAGA ACTTTCCGGA GCCGGAAGCG GAGGAAGCGG ATGCGAAACA CCCGGTTTTT GCGGTTATCG GCCGCCCGAA TGTGGGCAAG AGCACCCTGG TGAACGCGAT CCTGGGTGAA AAACGTGTTA TTGCGTTCGA CATGGCGGGC ACCACCCGTG ACAGCATCCA CATTGATTTC GAGCGTGAAG GCAAGCCGTT TACCATCATT GACACCGCGG GTGTGCGTCG TCGTGGCAAA GTTGATGAGG CGGTGGAAAA GTTCAGCGTT ATCAAAGCGA TGCAAGCGGT GGAGGCGGCG AACGTGGCGG TTCTGGTGCT GGACGCGCAG CAAGATATCG CGGACCAAGA TGCGACCATT GCGGGTTTTG CGCTGGAGGC GGGTCGTGCG CTGGTTGTGG CGGTTAACAA GTGGGACGGT ATCAGCGAGG AACGTCGTGA ACAGGTGAAG CGTGACATTA GCCGTAAACT GTACTTCCTG GATTTTGCGA AGTTCCACTT TATTAGCGCG CTGAAAGAGC GTGGTATCGA TGGCCTGTTC GAAAGCATTC AGGCGGCGTA TAACGCGGCG ATGATCAAGA TGCCGACCCC GAAAATTACC CGTGTTCTGC AAACCGCGGT GGGTCGTCAG CAACCGCCGC GTGCGGGCCT GGTTCGTCCG AAGATGCGTT ATGCGCACCA GGGTGGCATG AACCCGCCGG TGATCGTGGT TCACGGTAAC AGCCTGCACG CGATTAGCGA CAGCTACACC CGTTATCTGA CCCAAACCTT CCGTAAAGCG TTTAACCTGC AGGGCACCCC GCTGCGTATC CAATACAACG TGAGCGAAAA CCCGTATGAG AACGCGGAAG ATAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for NegoA.00852.a.TH11.PD38460
| PURIFICATION DATe: | 4/19/2021 |
| CONCENTRATION: | 0.7mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM HEPES pH 7.0, 150mM NaCl, 1mM TCEP, 10% glycerol |
| EXPRESSION HOST: | BL21(DE3) + pBirA (adds CmR) |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 500µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
CID103346, PUR2793, PID8994-1
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAEEADAKHP VFAVIGRPNV GKSTLVNAIL GEKRVIAFDM AGTTRDSIHI DFEREGKPFT IIDTAGVRRR GKVDEAVEKF SVIKAMQAVE AANVAVLVLD AQQDIADQDA TIAGFALEAG RALVVAVNKW DGISEERREQ VKRDISRKLY FLDFAKFHFI SALKERGIDG LFESIQAAYN AAMIKMPTPK ITRVLQTAVG RQQPPRAGLV RPKMRYAHQG GMNPPVIVVH GNSLHAISDS YTRYLTQTFR KAFNLQGTPL RIQYNVSENP YENAEDGLND IFEAQKIEWH EGSGSHHHHH HHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGCGGAGG AAGCGGATGC GAAACACCCG GTTTTTGCGG TTATCGGCCG CCCGAATGTG GGCAAGAGCA CCCTGGTGAA CGCGATCCTG GGTGAAAAAC GTGTTATTGC GTTCGACATG GCGGGCACCA CCCGTGACAG CATCCACATT GATTTCGAGC GTGAAGGCAA GCCGTTTACC ATCATTGACA CCGCGGGTGT GCGTCGTCGT GGCAAAGTTG ATGAGGCGGT GGAAAAGTTC AGCGTTATCA AAGCGATGCA AGCGGTGGAG GCGGCGAACG TGGCGGTTCT GGTGCTGGAC GCGCAGCAAG ATATCGCGGA CCAAGATGCG ACCATTGCGG GTTTTGCGCT GGAGGCGGGT CGTGCGCTGG TTGTGGCGGT TAACAAGTGG GACGGTATCA GCGAGGAACG TCGTGAACAG GTGAAGCGTG ACATTAGCCG TAAACTGTAC TTCCTGGATT TTGCGAAGTT CCACTTTATT AGCGCGCTGA AAGAGCGTGG TATCGATGGC CTGTTCGAAA GCATTCAGGC GGCGTATAAC GCGGCGATGA TCAAGATGCC GACCCCGAAA ATTACCCGTG TTCTGCAAAC CGCGGTGGGT CGTCAGCAAC CGCCGCGTGC GGGCCTGGTT CGTCCGAAGA TGCGTTATGC GCACCAGGGT GGCATGAACC CGCCGGTGAT CGTGGTTCAC GGTAACAGCC TGCACGCGAT TAGCGACAGC TACACCCGTT ATCTGACCCA AACCTTCCGT AAAGCGTTTA ACCTGCAGGG CACCCCGCTG CGTATCCAAT ACAACGTGAG CGAAAACCCG TATGAGAACG CGGAAGATGG CCTGAACGAC ATCTTCGAGG CTCAGAAAAT CGAATGGCAC GAAGGCTCTG GTAGCCATCA TCATCACCAT CACCACCACT GATAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T
Details for NegoA.00852.a.AE7.PW38937
| PURIFICATION DATe: | 2/3/2021 |
| CONCENTRATION: | 36mg/ml |
| OBSERVED MW: | 51kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVFSSTERHL TQKDPIMKPT IALIGRPNVG KSTLFNRLTR TKDALVHDLP GLTRDRHYGH GKVGSKPYFV IDTGGFEPVV DSGILHEMAK QTLQAVDEAD AVVFLVDGRT GLTPQDKIIA DRLRQSPRPV YLAVNKGEGG DRAVLAAEFY ELALGEPHVI SGAHGDGVYY LIEEILENFP EPEAEEADAK HPVFAVIGRP NVGKSTLVNA ILGEKRVIAF DMAGTTRDSI HIDFEREGKP FTIIDTAGVR RRGKVDEAVE KFSVIKAMQA VEAANVAVLV LDAQQDIADQ DATIAGFALE AGRALVVAVN KWDGISEERR EQVKRDISRK LYFLDFAKFH FISALKERGI DGLFESIQAA YNAAMIKMPT PKITRVLQTA VERQQPPRAG LVRPKMRYAH QGGMNPPVIV VHGNSLHAIS DSYTRYLTQT FRKAFNLQGT PLRIQYNVSE NPYENAEDAG HHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgtcttccgc attttcatac gggttttccg aaacattgta ttgaatccgc aacggcgtgc cttgcaggtt gaaggctttg cggaaggtct gggtcagata gcgcgtatag ctgtcggaaa tcgcgtgcag cgaattgccg tgaaccacaa tcaccggcgg attcatgccg ccttggtggg cataacgcat tttcggacgc accaagccgg cacgcggcgg ctgttggcgt tcgacggcgg tttgcaatac gcgcgtgatt ttcggcgtcg gcatcttaat catcgccgcg ttgtaggcag cttgaatgct ttcaaacaaa ccgtctatac cgcgctcttt caacgcggaa ataaagtgga acttggcaaa atcgaggaaa tacagtttgc gggaaatgtc gcgtttcact tgttcgcgcc gctcttcgct gatgccgtcc catttgttga cggcaactac cagcgcgcgt cccgcttcca aagcaaaacc ggcaatcgtc gcatcttggt cggcaatgtc ctgttgcgcg tccaatacca aaacagcaac gtttgccgct tcaaccgcct gcatggcttt gataacggag aacttttcca ccgcttcatc cactttgccg cgacggcgca cgcctgcggt atcgatgatg gtaaacggtt tgccttcgcg ctcgaaatcg atgtggatgc tgtcgcgcgt tgtgcctgcc atatcgaagg cgatgacgcg tttttcgccg agaatggcgt taaccagcgt agatttgccg acgttcggac gaccgataac ggcaaaaacg ggatgttttg catcggcttc ttcggcttca ggctcgggga agttttctaa aatttcttca atcaggtaat acacgccgtc gccgtgcgcg ccggaaataa cgtgcggctc gcccaatgcc agctcgtaaa actcggcggc aagtacggcc ctgtcgccgc cctcgccttt attcacggcc aaataaacgg gacgcggact ttggcgcaaa cggtcggcaa taatcttgtc ttgcggtgtt aaaccggtac ggccgtccac caaaaacaca accgcatcgg cttcatcgac agcctgcaag gtttgttttg ccatttcgtg caaaatgccg ctgtccacaa ccggctcgaa accgccggta tcgatgacaa aataaggttt gctgccgact ttgccgtgtc cgtaatggcg gtcgcgggtc agacccggca ggtcatgcac gagcgcgtct ttggtgcgcg tcaaacggtt gaacaaggta gatttgccga cgttggggcg gccgataagc gcgatggttg gtttcatgat tgggtctttc tgtgtcaagt gccgttcggt agaactgaac accatgggag aga
Expressed Protein Sequence
MFSSTERHLT QKDPIMKPTI ALIGRPNVGK STLFNRLTRT KDALVHDLPG LTRDRHYGHG KVGSKPYFVI DTGGFEPVVD SGILHEMAKQ TLQAVDEADA VVFLVDGRTG LTPQDKIIAD RLRQSPRPVY LAVNKGEGGD RAVLAAEFYE LALGEPHVIS GAHGDGVYYL IEEILENFPE PEAEEADAKH PVFAVIGRPN VGKSTLVNAI LGEKRVIAFD MAGTTRDSIH IDFEREGKPF TIIDTAGVRR RGKVDEAVEK FSVIKAMQAV EAANVAVLVL DAQQDIADQD ATIAGFALEA GRALVVAVNK WDGISEERRE QVKRDISRKL YFLDFAKFHF ISALKERGID GLFESIQAAY NAAMIKMPTP KITRVLQTAV GRQQPPRAGL VRPKMRYAHQ GGMNPPVIVV HGNSLHAISD SYTRYLTQTF RKAFNLQGTP LRIQYNVSEN PYENAEDGHH HHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCGT GTTCAGTTCT ACCGAACGGC ACTTGACACA GAAAGACCCA ATCATGAAAC CAACCATCGC GCTTATCGGC CGCCCCAACG TCGGCAAATC TACCTTGTTC AACCGTTTGA CGCGCACCAA AGACGCGCTC GTGCATGACC TGCCGGGTCT GACCCGCGAC CGCCATTACG GACACGGCAA AGTCGGCAGC AAACCTTATT TTGTCATCGA TACCGGCGGT TTCGAGCCGG TTGTGGACAG CGGCATTTTG CACGAAATGG CAAAACAAAC CTTGCAGGCT GTCGATGAAG CCGATGCGGT TGTGTTTTTG GTGGACGGCC GTACCGGTTT AACACCGCAA GACAAGATTA TTGCCGACCG TTTGCGCCAA AGTCCGCGTC CCGTTTATTT GGCCGTGAAT AAAGGCGAGG GCGGCGACAG GGCCGTACTT GCCGCCGAGT TTTACGAGCT GGCATTGGGC GAGCCGCACG TTATTTCCGG CGCGCACGGC GACGGCGTGT ATTACCTGAT TGAAGAAATT TTAGAAAACT TCCCCGAGCC TGAAGCCGAA GAAGCCGATG CAAAACATCC CGTTTTTGCC GTTATCGGTC GTCCGAACGT CGGCAAATCT ACGCTGGTTA ACGCCATTCT CGGCGAAAAA CGCGTCATCG CCTTCGATAT GGCAGGCACA ACGCGCGACA GCATCCACAT CGATTTCGAG CGCGAAGGCA AACCGTTTAC CATCATCGAT ACCGCAGGCG TGCGCCGTCG CGGCAAAGTG GATGAAGCGG TGGAAAAGTT CTCCGTTATC AAAGCTATGC AGGCGGTTGA AGCGGCAAAC GTTGCTGTTT TGGTATTGGA CGCGCAACAG GACATTGCCG ACCAAGATGC GACGATTGCC GGTTTTGCTT TGGAAGCGGG ACGCGCGCTG GTAGTTGCCG TCAACAAATG GGACGGCATC AGCGAAGAGC GGCGCGAACA AGTGAAACGC GACATTTCCC GCAAACTGTA TTTCCTCGAT TTTGCCAAGT TCCACTTTAT TTCCGCGTTG AAAGAGCGCG GTATAGACGG TTTGTTTGAA AGCATTCAAG CTGCCTACAA CGCGGCGATG ATTAAGATGC CGACGCCGAA AATCACGCGC GTATTGCAAA CCGCCGTCGG ACGCCAACAG CCGCCGCGTG CCGGCTTGGT GCGTCCGAAA ATGCGTTATG CCCACCAAGG CGGCATGAAT CCGCCGGTGA TTGTGGTACA CGGCAATTCG CTGCACGCGA TTTCCGACAG CTATACGCGC TATCTGACCC AGACCTTCCG CAAAGCCTTC AACCTGCAAG GCACGCCGTT GCGGATTCAA TACAATGTTT CGGAAAACCC GTATGAAAAT GCGGAAGACG GGCACCACCA TCATCATCAT TAACGGATCC GAATTCGAGC TCCGTCGACA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT