NegoA.00799.a
aminopeptidase A
| CENTER ID: | NegoA.00799.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00799.a.B1.GE39975 | full length | 1 | 468 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00799.a.B1.PW37906 | full length | 1 | 468 |
Structures
8D1X
DEPOSITED: 5/27/2022
DETERMINATION: XRay
CLONE: NegoA.00799.a.B1.GE39975
PROTEIN: NegoA.00799.a.B1.PW37906
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_85039 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.314 |
| UniProt: | B4RJ22 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEFSTKTEIL QEQQAGAQLF VCADKAPEHN TAAHALFSAL EEGQNFSDTK IPTDNGLQAV AVVRLEKTDR AALNKAAAEA AKWAQNQETV NVDVHAFDEA QAAAVAEAFA IAFGNAAYRF DRYKKEAKPA KFSQAVFHSA HEAAVKEALR VAEAQVYGQS LCRDLGNAAP NECTPEFLAR TAKAEAEKLG AHAKIIEKDY IKENMGSFWS VAKGSVEDPY LVELSYFGAA DKEAAPVVLV GKGITFDTGG ISLKPGLNMD EMKFDMCGAA TVISTFCAAV KLQLPINLIA IVATCENMPS GAANKPGDVV KSMKGLTIEV LNTDAEGRLI LCDALTYAEQ FKPKAVIDVA TLTGACIVAL GHDVSGVMGN NQDLIDSLLA ASYNVDDKAW QLPLFETYKD QLKSNFADIP NIGTPGAGTI TAATFLSYFT EGYPWAHLDI AGTAWKSGAE KGATGRPVPL LMNYLRNL
NT Sequence
GTGGAATTTA GCACAAAAAC CGAAATCTTG CAGGAACAGC AGGCAGGCGC GCAGTTATTT GTCTGTGCCG ACAAAGCACC CGAGCACAAC ACCGCCGCAC ATGCGCTCTT CTCTGCTTTG GAAGAGGGTC AGAATTTTTC CGACACCAAA ATCCCGACGG ACAACGGTTT GCAGGCAGTC GCCGTCGTCC GCCTCGAAAA AACCGACCGC GCCGCGCTGA ACAAAGCCGC CGCCGAAGCC GCCAAATGGG CGCAAAATCA AGAAACGGTC AATGTGGACG TTCACGCCTT TGATGAGGCG CAAGCAGCAG CCGTTGCCGA AGCTTTTGCC ATCGCGTTCG GCAACGCTGC ATATCGTTTC GACCGCTACA AAAAAGAAGC CAAGCCCGCC AAATTTTCGC AAGCCGTGTT CCACAGCGCA CACGAAGCCG CCGTCAAAGA AGCCCTGCGC GTTGCCGAAG CACAAGTTTA CGGACAAAGC CTCTGCCGCG ACTTAGGCAA TGCCGCACCG AACGAATGCA CGCCTGAGTT CCTCGCACGT ACCGCCAAAG CCGAAGCCGA GAAACTGGGC GCGCACGCCA AAATCATCGA AAAAGACTAC ATCAAAGAAA ACATGGGTTC GTTTTGGTCG GTTGCCAAAG GCAGCGTCGA AGACCCATAT TTGGTTGAAC TGAGCTATTT CGGTGCGGCC GACAAAGAAG CCGCGCCTGT GGTATTGGTC GGCAAAGGCA TTACCTTCGA CACCGGCGGC ATTTCCCTCA AACCCGGCCT GAACATGGAC GAAATGAAGT TTGACATGTG CGGCGCGGCA ACCGTCATCA GTACCTTCTG CGCCGCCGTC AAACTGCAAC TGCCGATCAA TCTGATTGCC ATCGTCGCCA CTTGTGAAAA CATGCCTTCC GGTGCAGCCA ACAAACCGGG CGATGTTGTG AAAAGTATGA AAGGGCTGAC GATTGAAGTG TTGAACACCG ATGCCGAAGG CCGTCTGATT TTGTGCGACG CGCTCACTTA CGCCGAGCAG TTCAAACCCA AAGCCGTCAT CGATGTCGCC ACCCTGACCG GCGCGTGCAT CGTCGCCTTG GGGCACGATG TCAGCGGCGT GATGGGCAAT AATCAGGATT TGATCGACAG CCTGCTTGCC GCTTCCTACA ACGTGGACGA CAAAGCGTGG CAACTGCCGC TCTTTGAAAC CTACAAAGAC CAGCTCAAAT CCAACTTTGC CGACATCCCC AATATCGGCA CGCCCGGCGC GGGCACGATT ACCGCCGCAA CATTCCTGTC CTATTTCACC GAAGGCTACC CGTGGGCGCA CCTTGACATC GCGGGTACGG CGTGGAAATC CGGAGCGGAA AAAGGTGCGA CCGGCCGCCC CGTACCGCTG CTGATGAACT ATCTGCGGAA TCTTTAA
Details for NegoA.00799.a.B1.GE39975
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMV EFSTKTEILQ EQQAGAQLFV CADKAPEHNT AAHALFSALE EGQNFSDTKI PTDNGLQAVA VVRLEKTDRA ALNKAAAEAA KWAQNQETVN VDVHAFDEAQ AAAVAEAFAI AFGNAAYRFD RYKKEAKPAK FSQAVFHSAH EAAVKEALRV AEAQVYGQSL CRDLGNAAPN ECTPEFLART AKAEAEKLGA HAKIIEKDYI KENMGSFWSV AKGSVEDPYL VELSYFGAAD KEAAPVVLVG KGITFDTGGI SLKPGLNMDE MKFDMCGAAT VISTFCAAVK LQLPINLIAI VATCENMPSG AANKPGDVVK SMKGLTIEVL NTDAEGRLIL CDALTYAEQF KPKAVIDVAT LTGACIVALG HDVSGVMGNN QDLIDSLLAA SYNVDDKAWQ LPLFETYKDQ LKSNFADIPN IGTPGAGTIT AATFLSYFTE GYPWAHLDIA GTAWKSGAEK GATGRPV
Validated NT Sequence
ggtacggggc ggccggtcgc acctttttcc gctccggatt tccacgccgt acccgcgatg tcaaggtgcg cccacgggta gccttcggtg aaataggaca ggaatgttgc ggcggtaatc gtgcccgcgc cgggcgtgcc gatattgggg atgtcggcaa agttggattt gagctggtct ttgtaggttt caaagagcgg cagttgccac gctttgtcgt ccacgttgta ggaagcggca agcaggctgt cgatcaaatc ctgattattg cccatcacgc cgctgacatc gtgccccaag gcgacgatgc acgcgccggt cagggtggcg acatcgatga cggctttggg tttgaactgc tcggcgtaag tgagcgcgtc gcacaaaatc agacggcctt cggcatcggt gttcaacact tcaatcgtca gccctttcat acttttcaca acatcgcccg gtttgtttgc cgcgccggaa ggcatgtttt cacaagtggc gacgatggca atcagattga tcggcagttg cagtttgacg gcggcgcaga aggtgctgat gacggttgcc gcgccgcaca tgtcaaactt catttcgtcc atgttcaggc cgggtttgag ggaaatgccg ccggtgtcga aggtaatgcc tttgccgacc aataccacag gcgcggcttc tttgtcggcc gcaccgaaat agctcagttc aaccaaatat gggtcttcga cgctgccttt ggcaaccgac caaaacgaac ccatgttttc tttgatgtag tctttttcga tgattttggc gtgcgcgccc agtttctcgg cttcggcttt ggcggtacgc gcgaggaact caggcgtgca ttcgttcggt gcggcattgc ctaagtcgcg gcagaggctt tgtccgtaaa cttgtgcttc ggcaacgcgc agggcttctt tgacggcggc ttcgtgtgcg ctgtggaaca cggcttgcga aaatttggcg ggcttggctt cttttttgta gcggtcgaaa cgatatgcag cgttgccgaa cgcgatggca aaagcttcgg caacggctgc tgcttgcgcc tcatcaaagg cgtgaacgtc cacattgacc gtttcttgat tttgcgccca tttggcggct tcggcggcgg ctttgttcag cgcggcgcgg tcggtttttt cgaggcggac gacggcgact gcctgcaaac cgttgtccgt cgggattttg gtgtcggaaa aattctgacc ctcttccaaa gcagagaaga gcgcatgtgc ggcggtgttg tgctcgggtg ctttgtcggc acagacaaat aactgcgcgc ctgcctgctg ttcctgcaag atttcggttt ttgtgctaaa ttccaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHME FSTKTEILQE QQAGAQLFVC ADKAPEHNTA AHALFSALEE GQNFSDTKIP TDNGLQAVAV VRLEKTDRAA LNKAAAEAAK WAQNQETVNV DVHAFDEAQA AAVAEAFAIA FGNAAYRFDR YKKEAKPAKF SQAVFHSAHE AAVKEALRVA EAQVYGQSLC RDLGNAAPNE CTPEFLARTA KAEAEKLGAH AKIIEKDYIK ENMGSFWSVA KGSVEDPYLV ELSYFGAADK EAAPVVLVGK GITFDTGGIS LKPGLNMDEM KFDMCGAATV ISTFCAAVKL QLPINLIAIV ATCENMPSGA ANKPGDVVKS MKGLTIEVLN TDAEGRLILC DALTYAEQFK PKAVIDVATL TGACIVALGH DVSGVMGNNQ DLIDSLLAAS YNVDDKAWQL PLFETYKDQL KSNFADIPNI GTPGAGTITA ATFLSYFTEG YPWAHLDIAG TAWKSGAEKG ATGRPVPLLM NYLRNL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggaat ttagcacaaa aaccgaaatc ttgcaggaac agcaggcagg cgcgcagtta tttgtctgtg ccgacaaagc acccgagcac aacaccgccg cacatgcgct cttctctgct ttggaagagg gtcagaattt ttccgacacc aaaatcccga cggacaacgg tttgcaggca gtcgccgtcg tccgcctcga aaaaaccgac cgcgccgcgc tgaacaaagc cgccgccgaa gccgccaaat gggcgcaaaa tcaagaaacg gtcaatgtgg acgttcacgc ctttgatgag gcgcaagcag cagccgttgc cgaagctttt gccatcgcgt tcggcaacgc tgcatatcgt ttcgaccgct acaaaaaaga agccaagccc gccaaatttt cgcaagccgt gttccacagc gcacacgaag ccgccgtcaa agaagccctg cgcgttgccg aagcacaagt ttacggacaa agcctctgcc gcgacttagg caatgccgca ccgaacgaat gcacgcctga gttcctcgca cgtaccgcca aagccgaagc cgagaaactg ggcgcgcacg ccaaaatcat cgaaaaagac tacatcaaag aaaacatggg ttcgttttgg tcggttgcca aaggcagcgt cgaagaccca tatttggttg aactgagcta tttcggtgcg gccgacaaag aagccgcgcc tgtggtattg gtcggcaaag gcattacctt cgacaccggc ggcatttccc tcaaacccgg cctgaacatg gacgaaatga agtttgacat gtgcggcgcg gcaaccgtca tcagtacctt ctgcgccgcc gtcaaactgc aactgccgat caatctgatt gccatcgtcg ccacttgtga aaacatgcct tccggtgcag ccaacaaacc gggcgatgtt gtgaaaagta tgaaagggct gacgattgaa gtgttgaaca ccgatgccga aggccgtctg attttgtgcg acgcgctcac ttacgccgag cagttcaaac ccaaagccgt catcgatgtc gccaccctga ccggcgcgtg catcgtcgcc ttggggcacg atgtcagcgg cgtgatgggc aataatcagg atttgatcga cagcctgctt gccgcttcct acaacgtgga cgacaaagcg tggcaactgc cgctctttga aacctacaaa gaccagctca aatccaactt tgccgacatc cccaatatcg gcacgcccgg cgcgggcacg attaccgccg caacattcct gtcctatttc accgaaggct acccgtgggc gcaccttgac atcgcgggta cggcgtggaa atccggagcg gaaaaaggtg cgaccggccg ccccgtaccg ctgctgatga actatctgcg gaatctttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for NegoA.00799.a.B1.PW37906
| PURIFICATION DATe: | 5/11/2016 |
| CONCENTRATION: | 38mg/ml |
| OBSERVED MW: | 51kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV EFSTKTEILQ EQQAGAQLFV CADKAPEHNT AAHALFSALE EGQNFSDTKI PTDNGLQAVA VVRLEKTDRA ALNKAAAEAA KWAQNQETVN VDVHAFDEAQ AAAVAEAFAI AFGNAAYRFD RYKKEAKPAK FSQAVFHSAH EAAVKEALRV AEAQVYGQSL CRDLGNAAPN ECTPEFLART AKAEAEKLGA HAKIIEKDYI KENMGSFWSV AKGSVEDPYL VELSYFGAAD KEAAPVVLVG KGITFDTGGI SLKPGLNMDE MKFDMCGAAT VISTFCAAVK LQLPINLIAI VATCENMPSG AANKPGDVVK SMKGLTIEVL NTDAEGRLIL CDALTYAEQF KPKAVIDVAT LTGACIVALG HDVSGVMGNN QDLIDSLLAA SYNVDDKAWQ LPLFETYKDQ LKSNFADIPN IGTPGAGTIT AATFLSYFTE GYPWAHLDIA GTAWKSGAEK GATGRPV
Validated NT Sequence
ggtacggggc ggccggtcgc acctttttcc gctccggatt tccacgccgt acccgcgatg tcaaggtgcg cccacgggta gccttcggtg aaataggaca ggaatgttgc ggcggtaatc gtgcccgcgc cgggcgtgcc gatattgggg atgtcggcaa agttggattt gagctggtct ttgtaggttt caaagagcgg cagttgccac gctttgtcgt ccacgttgta ggaagcggca agcaggctgt cgatcaaatc ctgattattg cccatcacgc cgctgacatc gtgccccaag gcgacgatgc acgcgccggt cagggtggcg acatcgatga cggctttggg tttgaactgc tcggcgtaag tgagcgcgtc gcacaaaatc agacggcctt cggcatcggt gttcaacact tcaatcgtca gccctttcat acttttcaca acatcgcccg gtttgtttgc cgcgccggaa ggcatgtttt cacaagtggc gacgatggca atcagattga tcggcagttg cagtttgacg gcggcgcaga aggtgctgat gacggttgcc gcgccgcaca tgtcaaactt catttcgtcc atgttcaggc cgggtttgag ggaaatgccg ccggtgtcga aggtaatgcc tttgccgacc aataccacag gcgcggcttc tttgtcggcc gcaccgaaat agctcagttc aaccaaatat gggtcttcga cgctgccttt ggcaaccgac caaaacgaac ccatgttttc tttgatgtag tctttttcga tgattttggc gtgcgcgccc agtttctcgg cttcggcttt ggcggtacgc gcgaggaact caggcgtgca ttcgttcggt gcggcattgc ctaagtcgcg gcagaggctt tgtccgtaaa cttgtgcttc ggcaacgcgc agggcttctt tgacggcggc ttcgtgtgcg ctgtggaaca cggcttgcga aaatttggcg ggcttggctt cttttttgta gcggtcgaaa cgatatgcag cgttgccgaa cgcgatggca aaagcttcgg caacggctgc tgcttgcgcc tcatcaaagg cgtgaacgtc cacattgacc gtttcttgat tttgcgccca tttggcggct tcggcggcgg ctttgttcag cgcggcgcgg tcggtttttt cgaggcggac gacggcgact gcctgcaaac cgttgtccgt cgggattttg gtgtcggaaa aattctgacc ctcttccaaa gcagagaaga gcgcatgtgc ggcggtgttg tgctcgggtg ctttgtcggc acagacaaat aactgcgcgc ctgcctgctg ttcctgcaag atttcggttt ttgtgctaaa ttccaccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHME FSTKTEILQE QQAGAQLFVC ADKAPEHNTA AHALFSALEE GQNFSDTKIP TDNGLQAVAV VRLEKTDRAA LNKAAAEAAK WAQNQETVNV DVHAFDEAQA AAVAEAFAIA FGNAAYRFDR YKKEAKPAKF SQAVFHSAHE AAVKEALRVA EAQVYGQSLC RDLGNAAPNE CTPEFLARTA KAEAEKLGAH AKIIEKDYIK ENMGSFWSVA KGSVEDPYLV ELSYFGAADK EAAPVVLVGK GITFDTGGIS LKPGLNMDEM KFDMCGAATV ISTFCAAVKL QLPINLIAIV ATCENMPSGA ANKPGDVVKS MKGLTIEVLN TDAEGRLILC DALTYAEQFK PKAVIDVATL TGACIVALGH DVSGVMGNNQ DLIDSLLAAS YNVDDKAWQL PLFETYKDQL KSNFADIPNI GTPGAGTITA ATFLSYFTEG YPWAHLDIAG TAWKSGAEKG ATGRPVPLLM NYLRNL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGAAT TTAGCACAAA AACCGAAATC TTGCAGGAAC AGCAGGCAGG CGCGCAGTTA TTTGTCTGTG CCGACAAAGC ACCCGAGCAC AACACCGCCG CACATGCGCT CTTCTCTGCT TTGGAAGAGG GTCAGAATTT TTCCGACACC AAAATCCCGA CGGACAACGG TTTGCAGGCA GTCGCCGTCG TCCGCCTCGA AAAAACCGAC CGCGCCGCGC TGAACAAAGC CGCCGCCGAA GCCGCCAAAT GGGCGCAAAA TCAAGAAACG GTCAATGTGG ACGTTCACGC CTTTGATGAG GCGCAAGCAG CAGCCGTTGC CGAAGCTTTT GCCATCGCGT TCGGCAACGC TGCATATCGT TTCGACCGCT ACAAAAAAGA AGCCAAGCCC GCCAAATTTT CGCAAGCCGT GTTCCACAGC GCACACGAAG CCGCCGTCAA AGAAGCCCTG CGCGTTGCCG AAGCACAAGT TTACGGACAA AGCCTCTGCC GCGACTTAGG CAATGCCGCA CCGAACGAAT GCACGCCTGA GTTCCTCGCA CGTACCGCCA AAGCCGAAGC CGAGAAACTG GGCGCGCACG CCAAAATCAT CGAAAAAGAC TACATCAAAG AAAACATGGG TTCGTTTTGG TCGGTTGCCA AAGGCAGCGT CGAAGACCCA TATTTGGTTG AACTGAGCTA TTTCGGTGCG GCCGACAAAG AAGCCGCGCC TGTGGTATTG GTCGGCAAAG GCATTACCTT CGACACCGGC GGCATTTCCC TCAAACCCGG CCTGAACATG GACGAAATGA AGTTTGACAT GTGCGGCGCG GCAACCGTCA TCAGTACCTT CTGCGCCGCC GTCAAACTGC AACTGCCGAT CAATCTGATT GCCATCGTCG CCACTTGTGA AAACATGCCT TCCGGTGCAG CCAACAAACC GGGCGATGTT GTGAAAAGTA TGAAAGGGCT GACGATTGAA GTGTTGAACA CCGATGCCGA AGGCCGTCTG ATTTTGTGCG ACGCGCTCAC TTACGCCGAG CAGTTCAAAC CCAAAGCCGT CATCGATGTC GCCACCCTGA CCGGCGCGTG CATCGTCGCC TTGGGGCACG ATGTCAGCGG CGTGATGGGC AATAATCAGG ATTTGATCGA CAGCCTGCTT GCCGCTTCCT ACAACGTGGA CGACAAAGCG TGGCAACTGC CGCTCTTTGA AACCTACAAA GACCAGCTCA AATCCAACTT TGCCGACATC CCCAATATCG GCACGCCCGG CGCGGGCACG ATTACCGCCG CAACATTCCT GTCCTATTTC ACCGAAGGCT ACCCGTGGGC GCACCTTGAC ATCGCGGGTA CGGCGTGGAA ATCCGGAGCG GAAAAAGGTG CGACCGGCCG CCCCGTACCG CTGCTGATGA ACTATCTGCG GAATCTTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT