NegoA.00784.a

thioredoxin-disulfide reductase

CENTER ID: NegoA.00784.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.00784.a.B2.GE40037 mature protein 24 316
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_85038
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|521006.8.peg.1437
UniProt: B4RMI1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQHRKLIIL GSGPAGYTAA VYAARANLNP VIITGIAQGG QLMTTTEVDN WPADADGVQG PELMARFLAH AERFGTEIIF DQINAVDLQK RPFALKGDMG EYTCDALIVA TGASAKYLGL PSEEAFAGKG VSACATCDGF FYKNQDVAVV GGGNTAVEEA LYLANIAKTV TLIHRRSEFR AEKIMIDKLM KRVEEGKIIL KLESNLQEVL GDDRGVNGAL LKNNDGSDQQ IAVSGIFIAI GHKPNTDIFK GQLEMDEAGY LKTKGGTADN VGATNIEGVW AAGDVKDHTY RQAITSAASG CQAALDAERW LGSQNI
NT Sequence
ATGTCCCAAC ACCGCAAACT GATTATTTTG GGTTCCGGCC CCGCCGGATA CACCGCCGCC GTCTATGCCG CACGTGCCAA TTTAAACCCC GTCATTATTA CAGGTATCGC GCAAGGCGGG CAACTGATGA CAACGACTGA AGTGGACAAC TGGCCTGCCG ATGCCGACGG CGTGCAAGGG CCGGAATTGA TGGCGCGGTT TCTCGCCCAT GCCGAACGTT TCGGGACAGA AATCATTTTT GACCAAATCA ACGCCGTCGA TCTGCAAAAA CGCCCGTTCG CACTCAAAGG CGATATGGGC GAGTACACTT GCGATGCCCT GATTGTCGCC ACCGGCGCGT CCGCCAAATA CCTTGGTTTG CCGAGTGAGG AAGCGTTTGC AGGGAAAGGC GTTTCCGCCT GCGCCACCTG CGACGGTTTC TTCTACAAAA ATCAAGATGT TGCCGTAGTC GGCGGCGGCA ATACGGCAGT TGAGGAGGCA CTCTACCTTG CCAATATCGC CAAAACCGTT ACGCTGATTC ACCGCCGCAG CGAGTTCCGT GCTGAAAAAA TCATGATTGA CAAACTGATG AAACGCGTGG AAGAGGGCAA AATCATCCTC AAGCTGGAAA GCAACCTGCA AGAAGTACTG GGCGACGATC GGGGCGTAAA TGGCGCATTA TTAAAAAACA ACGACGGTTC TGATCAACAA ATTGCCGTCA GCGGCATCTT TATCGCCATC GGGCACAAGC CGAATACCGA TATTTTCAAA GGGCAGTTGG AAATGGACGA AGCCGGCTAC CTGAAAACAA AAGGCGGCAC GGCGGACAAT GTCGGTGCAA CCAATATCGA AGGCGTATGG GCGGCGGGCG ACGTAAAAGA CCATACCTAC CGTCAGGCAA TTACCAGCGC GGCCTCCGGC TGTCAGGCGG CTTTGGACGC GGAACGTTGG CTGGGCAGCC AAAACATTTG A
Details for NegoA.00784.a.B2.GE40037
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA RANLNPVIIT GIAQGGQLMT TTEVDNWPAD ADGVQGPELM ARFLAHAERF GTEIIFDQIN AVDLQKRPFA LKGDMGEYTC DALIVATGAS AKYLGLPSEE AFAGKGVSAC ATCDGFFYKN QDVAVVGGGN TAVEEALYLA NIAKTVTLIH RRSEFRAEKI MIDKLMKRVE EGKIILKLES NLQEVLGDDR GVNGALLKNN DGSDQQIAVS GIFIAIGHKP NTDIFKGQLE MDEAGYLKTK GGTADNVGAT NIEGVWAAGD VKDHTYRQAI TSAASGCQAA LDAERWLGSQ NI
Validated NT Sequence
actcacttaa atgttttggc tgcccagcca acgttccgcg tccaaagccg cctgacagcc ggaggccgcg ctggtaattg cctgacggta ggtatggtct tttacgtcgc ccgccgccca tacgccttcg atattggttg caccgacatt gtccgccgtg ccgccttttg ttttcaggta gccggcttcg tccatttcca actgcccttt gaaaatatcg gtattcggct tgtgcccgat ggcgataaag atgccgctga cggcaatttg ttgatcagaa ccgtcgttgt tttttaataa tgcgccattt acgccccgat cgtcgcccag tacttcttgc aggttgcttt ccagcttgag gatgattttg ccctcttcca cgcgtttcat cagtttgtca atcatgattt tttcagcacg gaactcgctg cggcggtgaa tcagcgtaac ggttttggcg atattggcaa ggtagagtgc ctcctcaact gccgtattgc cgccgccgac tacggcaaca tcttgatttt tgtagaagaa accgtcgcag gtggcgcagg cggaaacgcc tttccctgca aacgcttcct cactcggcaa accaaggtat ttggcggacg cgccggtggc gacaatcagg gcatcgcaag tgtactcgcc catatcgcct ttgagtgcga acgggcgttt ttgcagatcg acggcgttga tttggtcaaa aatgatttcc gttccgaaac gttcggcgtg ggcgagaaac cgcgccatca attccggccc ttgcacgccg tcggcatcgg caggccagtt gtccacttca gtcgttgtca tcagttgccc gccttgcgcg atacctgtaa taatgacggg gtttaaattg gcacgtgcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHAR ANLNPVIITG IAQGGQLMTT TEVDNWPADA DGVQGPELMA RFLAHAERFG TEIIFDQINA VDLQKRPFAL KGDMGEYTCD ALIVATGASA KYLGLPSEEA FAGKGVSACA TCDGFFYKNQ DVAVVGGGNT AVEEALYLAN IAKTVTLIHR RSEFRAEKIM IDKLMKRVEE GKIILKLESN LQEVLGDDRG VNGALLKNND GSDQQIAVSG IFIAIGHKPN TDIFKGQLEM DEAGYLKTKG GTADNVGATN IEGVWAAGDV KDHTYRQAIT SAASGCQAAL DAERWLGSQN I
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcacgtg ccaatttaaa ccccgtcatt attacaggta tcgcgcaagg cgggcaactg atgacaacga ctgaagtgga caactggcct gccgatgccg acggcgtgca agggccggaa ttgatggcgc ggtttctcgc ccatgccgaa cgtttcggga cagaaatcat ttttgaccaa atcaacgccg tcgatctgca aaaacgcccg ttcgcactca aaggcgatat gggcgagtac acttgcgatg ccctgattgt cgccaccggc gcgtccgcca aataccttgg tttgccgagt gaggaagcgt ttgcagggaa aggcgtttcc gcctgcgcca cctgcgacgg tttcttctac aaaaatcaag atgttgccgt agtcggcggc ggcaatacgg cagttgagga ggcactctac cttgccaata tcgccaaaac cgttacgctg attcaccgcc gcagcgagtt ccgtgctgaa aaaatcatga ttgacaaact gatgaaacgc gtggaagagg gcaaaatcat cctcaagctg gaaagcaacc tgcaagaagt actgggcgac gatcggggcg taaatggcgc attattaaaa aacaacgacg gttctgatca acaaattgcc gtcagcggca tctttatcgc catcgggcac aagccgaata ccgatatttt caaagggcag ttggaaatgg acgaagccgg ctacctgaaa acaaaaggcg gcacggcgga caatgtcggt gcaaccaata tcgaaggcgt atgggcggcg ggcgacgtaa aagaccatac ctaccgtcag gcaattacca gcgcggcctc cggctgtcag gcggctttgg acgcggaacg ttggctgggc agccaaaaca tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat