NegoA.00743.a
tryptophanyl-tRNA synthetase
| CENTER ID: | NegoA.00743.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00743.a.B1.GE39974 | full length | 1 | 336 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00743.a.B1.PW38597 | full length | 1 | 336 |
Structures
5TEV
DEPOSITED: 9/22/2016
DETERMINATION: XRay
CLONE: NegoA.00743.a.B1.GE39974
PROTEIN: NegoA.00743.a.B1.PS37949
5TEW
DEPOSITED: 9/22/2016
DETERMINATION: XRay
CLONE: NegoA.00743.a.B1.GE39974
PROTEIN: NegoA.00743.a.B1.PS37949
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.804 |
| UniProt: | B4RKT1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKKRVLTGV TTTGTPHLGN YVGAIRPAVR AAQNPDTESF LFLADYHGII KCHEQEMIHQ STQAVAATWL ACGLDPERTT FYRQSDIPEV MELNWILTCI TAKGLMNRAH AYKAAVQANA ENGQEDPDFG VEMGLFSYPI LMTADILMFN ANEVPVGRDQ IQHVEMARDI AGRFNHRFQE LFTLPEVKID ENVELLVGLD GRKMSKSYGN TIPLWENDKK TQKSVNKIIT NMKEPGEPKQ PDESPLFEIY KAFSTPSETA EFTQMLADGL AWGEAKKLSA AKINAELAEL RERYNALTSN PSQIEEILQA GAQKARKEAR ELLDKVRDAV GIRPLK
NT Sequence
ATGAGCAAAA AACGAGTTCT GACCGGCGTA ACCACCACCG GCACCCCGCA TCTGGGCAAC TACGTCGGCG CCATCCGCCC CGCCGTCCGC GCGGCGCAAA ACCCCGATAC CGAATCCTTC CTTTTCCTCG CCGATTACCA CGGCATCATC AAATGCCACG AGCAGGAGAT GATTCACCAA TCCACCCAAG CCGTTGCCGC CACTTGGCTT GCCTGCGGAC TCGACCCCGA GCGCACCACC TTCTACCGCC AAAGCGACAT TCCCGAAGTG ATGGAGTTGA ACTGGATTCT GACCTGCATC ACCGCCAAGG GTTTGATGAA CCGCGCCCAC GCCTACAAAG CCGCCGTTCA AGCCAATGCG GAAAACGGGC AGGAAGACCC CGATTTCGGT GTGGAAATGG GTTTGTTCAG TTATCCGATT CTGATGACTG CCGACATTTT GATGTTCAAC GCCAACGAAG TCCCCGTCGG GCGCGACCAA ATCCAACACG TCGAAATGGC GCGCGACATT GCCGGCCGCT TCAACCACCG TTTCCAAGAA CTCTTCACCC TGCCCGAAGT GAAAATCGAC GAAAACGTCG AACTCTTGGT CGGTTTGGAC GGACGCAAAA TGTCCAAATC CTACGGCAAT ACCATTCCGC TTTGGGAAAA CGACAAAAAA ACCCAAAAAT CGGTCAACAA AATCATTACC AACATGAAAG AGCCGGGCGA GCCGAAACAG CCCGACGAAA GCCCTTTGTT TGAAATCTAT AAAGCCTTCT CCACGCCGTC TGAAACGGCG GAATTTACGC AAATGCTTGC CGACGGCTTG GCTTGGGGCG AGGCGAAAAA ACTTTCGGCG GCGAAAATCA ACGCCGAACT GGCAGAACTG CGCGAACGCT ACAACGCATT GACCTCTAAT CCTTCGCAAA TCGAAGAGAT TTTGCAGGCA GGCGCGCAAA AAGCGCGCAA AGAAGCGCGC GAATTGTTGG ATAAAGTACG CGATGCGGTC GGCATCCGCC CGTTGAAATA A
Details for NegoA.00743.a.B1.GE39974
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS KKRVLTGVTT TGTPHLGNYV GAIRPAVRAA QNPDTESFLF LADYHGIIKC HEQEMIHQST QAVAATWLAC GLDPERTTFY RQSDIPEVME LNWILTCITA KGLMNRAHAY KAAVQANAEN GQEDPDFGVE MGLFSYPILM TADILMFNAN EVPVGRDQIQ HVEMARDIAG RFNHRFQELF TLPEVKIDEN VELLVGLDGR KMSKSYGNTI PLWENDKKTQ KSVNKIITNM KEPGEPKQPD ESPLFEIYKA FSTPSETAEF TQMLADGLAW GEAKKLSAAK INAELAELRE RYNALTSNPS QIEEILQAGA QKARKEAREL LDKVRXXVGI RPLK
Validated NT Sequence
ttatttcaac gggcggatgc cgacnnnnnn ncgtacttta tccaacaatt cgcgcgcttc tttgcgcgct ttttgcgcgc ctgcctgcaa aatctcttcg atttgcgaag gattagaggt caatgcgttg tagcgttcgc gcagttctgc caattcggcg ttgattttcg ccgccgaaag ttttttcgcc tcgccccaag ccaagccgtc ggcaagcatt tgcgtaaatt ccgccgtttc agacggcgtg gagaaggctt tatagatttc aaacaaaggg ctttcgtcgg gctgtttcgg ctcgcccggc tctttcatgt tggtaatgat tttgttgacc gatttttggg tttttttgtc gttttcccaa agcggaatgg tattgccgta ggatttggac attttgcgtc cgtccaaacc gaccaagagt tcgacgtttt cgtcgatttt cacttcgggc agggtgaaga gttcttggaa acggtggttg aagcggccgg caatgtcgcg cgccatttcg acgtgttgga tttggtcgcg cccgacgggg acttcgttgg cgttgaacat caaaatgtcg gcagtcatca gaatcggata actgaacaaa cccatttcca caccgaaatc ggggtcttcc tgcccgtttt ccgcattggc ttgaacggcg gctttgtagg cgtgggcgcg gttcatcaaa cccttggcgg tgatgcaggt cagaatccag ttcaactcca tcacttcggg aatgtcgctt tggcggtaga aggtggtgcg ctcggggtcg agtccgcagg caagccaagt ggcggcaacg gcttgggtgg attggtgaat catctcctgc tcgtggcatt tgatgatgcc gtggtaatcg gcgaggaaga ggaaggattc ggtatcgggg ttttgcgccg cgcggacggc ggggcggatg gcgccgacgt agttgcccag atgcggggtg ccggtggtgg ttacgccggt cagaactcgt tttttgctca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS KKRVLTGVTT TGTPHLGNYV GAIRPAVRAA QNPDTESFLF LADYHGIIKC HEQEMIHQST QAVAATWLAC GLDPERTTFY RQSDIPEVME LNWILTCITA KGLMNRAHAY KAAVQANAEN GQEDPDFGVE MGLFSYPILM TADILMFNAN EVPVGRDQIQ HVEMARDIAG RFNHRFQELF TLPEVKIDEN VELLVGLDGR KMSKSYGNTI PLWENDKKTQ KSVNKIITNM KEPGEPKQPD ESPLFEIYKA FSTPSETAEF TQMLADGLAW GEAKKLSAAK INAELAELRE RYNALTSNPS QIEEILQAGA QKARKEAREL LDKVRDAVGI RPLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca aaaaacgagt tctgaccggc gtaaccacca ccggcacccc gcatctgggc aactacgtcg gcgccatccg ccccgccgtc cgcgcggcgc aaaaccccga taccgaatcc ttccttttcc tcgccgatta ccacggcatc atcaaatgcc acgagcagga gatgattcac caatccaccc aagccgttgc cgccacttgg cttgcctgcg gactcgaccc cgagcgcacc accttctacc gccaaagcga cattcccgaa gtgatggagt tgaactggat tctgacctgc atcaccgcca agggtttgat gaaccgcgcc cacgcctaca aagccgccgt tcaagccaat gcggaaaacg ggcaggaaga ccccgatttc ggtgtggaaa tgggtttgtt cagttatccg attctgatga ctgccgacat tttgatgttc aacgccaacg aagtccccgt cgggcgcgac caaatccaac acgtcgaaat ggcgcgcgac attgccggcc gcttcaacca ccgtttccaa gaactcttca ccctgcccga agtgaaaatc gacgaaaacg tcgaactctt ggtcggtttg gacggacgca aaatgtccaa atcctacggc aataccattc cgctttggga aaacgacaaa aaaacccaaa aatcggtcaa caaaatcatt accaacatga aagagccggg cgagccgaaa cagcccgacg aaagcccttt gtttgaaatc tataaagcct tctccacgcc gtctgaaacg gcggaattta cgcaaatgct tgccgacggc ttggcttggg gcgaggcgaa aaaactttcg gcggcgaaaa tcaacgccga actggcagaa ctgcgcgaac gctacaacgc attgacctct aatccttcgc aaatcgaaga gattttgcag gcaggcgcgc aaaaagcgcg caaagaagcg cgcgaattgt tggataaagt acgcgatgcg gtcggcatcc gcccgttgaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NegoA.00743.a.B1.PW38597
| PURIFICATION DATe: | 1/28/2019 |
| CONCENTRATION: | 36.65mg/ml |
| OBSERVED MW: | 39kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS KKRVLTGVTT TGTPHLGNYV GAIRPAVRAA QNPDTESFLF LADYHGIIKC HEQEMIHQST QAVAATWLAC GLDPERTTFY RQSDIPEVME LNWILTCITA KGLMNRAHAY KAAVQANAEN GQEDPDFGVE MGLFSYPILM TADILMFNAN EVPVGRDQIQ HVEMARDIAG RFNHRFQELF TLPEVKIDEN VELLVGLDGR KMSKSYGNTI PLWENDKKTQ KSVNKIITNM KEPGEPKQPD ESPLFEIYKA FSTPSETAEF TQMLADGLAW GEAKKLSAAK INAELAELRE RYNALTSNPS QIEEILQAGA QKARKEAREL LDKVRXXVGI RPLK
Validated NT Sequence
ttatttcaac gggcggatgc cgacnnnnnn ncgtacttta tccaacaatt cgcgcgcttc tttgcgcgct ttttgcgcgc ctgcctgcaa aatctcttcg atttgcgaag gattagaggt caatgcgttg tagcgttcgc gcagttctgc caattcggcg ttgattttcg ccgccgaaag ttttttcgcc tcgccccaag ccaagccgtc ggcaagcatt tgcgtaaatt ccgccgtttc agacggcgtg gagaaggctt tatagatttc aaacaaaggg ctttcgtcgg gctgtttcgg ctcgcccggc tctttcatgt tggtaatgat tttgttgacc gatttttggg tttttttgtc gttttcccaa agcggaatgg tattgccgta ggatttggac attttgcgtc cgtccaaacc gaccaagagt tcgacgtttt cgtcgatttt cacttcgggc agggtgaaga gttcttggaa acggtggttg aagcggccgg caatgtcgcg cgccatttcg acgtgttgga tttggtcgcg cccgacgggg acttcgttgg cgttgaacat caaaatgtcg gcagtcatca gaatcggata actgaacaaa cccatttcca caccgaaatc ggggtcttcc tgcccgtttt ccgcattggc ttgaacggcg gctttgtagg cgtgggcgcg gttcatcaaa cccttggcgg tgatgcaggt cagaatccag ttcaactcca tcacttcggg aatgtcgctt tggcggtaga aggtggtgcg ctcggggtcg agtccgcagg caagccaagt ggcggcaacg gcttgggtgg attggtgaat catctcctgc tcgtggcatt tgatgatgcc gtggtaatcg gcgaggaaga ggaaggattc ggtatcgggg ttttgcgccg cgcggacggc ggggcggatg gcgccgacgt agttgcccag atgcggggtg ccggtggtgg ttacgccggt cagaactcgt tttttgctca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS KKRVLTGVTT TGTPHLGNYV GAIRPAVRAA QNPDTESFLF LADYHGIIKC HEQEMIHQST QAVAATWLAC GLDPERTTFY RQSDIPEVME LNWILTCITA KGLMNRAHAY KAAVQANAEN GQEDPDFGVE MGLFSYPILM TADILMFNAN EVPVGRDQIQ HVEMARDIAG RFNHRFQELF TLPEVKIDEN VELLVGLDGR KMSKSYGNTI PLWENDKKTQ KSVNKIITNM KEPGEPKQPD ESPLFEIYKA FSTPSETAEF TQMLADGLAW GEAKKLSAAK INAELAELRE RYNALTSNPS QIEEILQAGA QKARKEAREL LDKVRDAVGI RPLK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA AAAAACGAGT TCTGACCGGC GTAACCACCA CCGGCACCCC GCATCTGGGC AACTACGTCG GCGCCATCCG CCCCGCCGTC CGCGCGGCGC AAAACCCCGA TACCGAATCC TTCCTTTTCC TCGCCGATTA CCACGGCATC ATCAAATGCC ACGAGCAGGA GATGATTCAC CAATCCACCC AAGCCGTTGC CGCCACTTGG CTTGCCTGCG GACTCGACCC CGAGCGCACC ACCTTCTACC GCCAAAGCGA CATTCCCGAA GTGATGGAGT TGAACTGGAT TCTGACCTGC ATCACCGCCA AGGGTTTGAT GAACCGCGCC CACGCCTACA AAGCCGCCGT TCAAGCCAAT GCGGAAAACG GGCAGGAAGA CCCCGATTTC GGTGTGGAAA TGGGTTTGTT CAGTTATCCG ATTCTGATGA CTGCCGACAT TTTGATGTTC AACGCCAACG AAGTCCCCGT CGGGCGCGAC CAAATCCAAC ACGTCGAAAT GGCGCGCGAC ATTGCCGGCC GCTTCAACCA CCGTTTCCAA GAACTCTTCA CCCTGCCCGA AGTGAAAATC GACGAAAACG TCGAACTCTT GGTCGGTTTG GACGGACGCA AAATGTCCAA ATCCTACGGC AATACCATTC CGCTTTGGGA AAACGACAAA AAAACCCAAA AATCGGTCAA CAAAATCATT ACCAACATGA AAGAGCCGGG CGAGCCGAAA CAGCCCGACG AAAGCCCTTT GTTTGAAATC TATAAAGCCT TCTCCACGCC GTCTGAAACG GCGGAATTTA CGCAAATGCT TGCCGACGGC TTGGCTTGGG GCGAGGCGAA AAAACTTTCG GCGGCGAAAA TCAACGCCGA ACTGGCAGAA CTGCGCGAAC GCTACAACGC ATTGACCTCT AATCCTTCGC AAATCGAAGA GATTTTGCAG GCAGGCGCGC AAAAAGCGCG CAAAGAAGCG CGCGAATTGT TGGATAAAGT ACGCGATGCG GTCGGCATCC GCCCGTTGAA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT