NegoA.00731.a
dihydrolipoyl dehydrogenase
| CENTER ID: | NegoA.00731.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00731.a.B1.GE39973 | full length | 1 | 594 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00731.a.B1.PW37934 | full length | 1 | 594 |
Structures
5U25
DEPOSITED: 11/29/2016
DETERMINATION: XRay
CLONE: NegoA.00731.a.B1.GE39973
PROTEIN: NegoA.00731.a.B1.PW37934
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.1462 |
| UniProt: | B4RMK2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALVELKVPD IGGHENVDII AVEVNVGDTI AVDDTLITLE TDKATMDVPA EVAGVIKEVK VKVGDKISEG GLIVVVEAEG AAAAPKAEAA AAPAQEAPKA AAPAPQAAQF GGAADAEYDV VVLGGGPGGY SAAFAAADEG LKVAIVERYK TLGGVCLNVG CIPSKALLHN AAVIDEVRHL AANGIKYPEP ELDIDMLRAY KDGVVSRLTG GLAGMAKSRK VDVIQGDGQF LDPHHLEVSL TAGDAYEQAA PTGEKKIVAF KNCIIAAGSR VTKLPFIPED PRIIDSSGAL ALKEVPGKLL IIGGGIIGLE MGTVYSTLGS RLDVVEMMDG LMQGADRDLV KVWQKQNEYR FDNIMVNTKT VAVEPKEDGV YVTFEGANAP KEPQRYDAVL VAAGRAPNGK LISAEKAGVA VTDRGFIEVD KQMRTNVPHI YAIGDIVGQP MLAHKAVHEG HVAAENCAGH KAYFDARVIP GVAYTSPEVA WVGETELSAK ASGRKITKAN FPWAASGRAI ANGCDNGFTK LIFDAETGRI IGGGIVGPNG GDMIGEVCLA IEMGCDAADI GKTIHPHPTL GESIGMAAEV ALGVCTDLPP QKKK
NT Sequence
ATGGCTTTAG TTGAATTGAA AGTGCCCGAC ATTGGCGGAC ACGAAAATGT AGATATTATC GCGGTTGAAG TGAACGTTGG CGACACCATC GCCGTTGACG ACACCCTGAT TACCTTGGAA ACCGACAAAG CGACGATGGA CGTGCCTGCC GAAGTTGCGG GCGTGATCAA AGAAGTGAAA GTCAAAGTCG GCGACAAAAT CTCCGAAGGC GGTTTGATTG TCGTCGTCGA AGCCGAAGGT GCGGCTGCCG CCCCTAAAGC CGAAGCGGCT GCCGCCCCGG CGCAAGAAGC ACCCAAAGCT GCCGCTCCTG CTCCGCAAGC CGCGCAATTC GGCGGTGCTG CCGATGCCGA GTACGACGTG GTCGTATTGG GCGGCGGTCC CGGCGGTTAC TCCGCTGCAT TTGCCGCTGC CGATGAAGGC TTGAAAGTCG CCATCGTCGA GCGTTACAAA ACTTTGGGCG GCGTTTGCCT GAACGTCGGC TGTATCCCTT CCAAAGCCTT GTTGCACAAT GCCGCCGTTA TCGACGAAGT GCGCCACTTG GCTGCCAACG GTATCAAATA CCCCGAACCG GAACTCGACA TCGATATGCT TCGCGCCTAC AAAGACGGCG TGGTTTCCCG CCTCACGGGT GGTTTGGCAG GTATGGCGAA AAGCCGTAAA GTGGACGTTA TCCAAGGCGA CGGGCAATTC TTGGATCCTC ACCACTTGGA AGTGTCGCTG ACTGCCGGCG ACGCGTACGA ACAGGCAGCC CCTACCGGCG AGAAAAAAAT CGTTGCCTTC AAAAACTGTA TCATTGCAGC AGGCAGCCGC GTAACCAAAC TGCCTTTCAT TCCTGAAGAT CCGCGCATCA TCGATTCCAG CGGCGCACTG GCACTGAAAG AAGTACCGGG CAAACTGCTG ATTATCGGCG GCGGCATTAT CGGCCTCGAG ATGGGTACGG TTTACAGCAC GCTGGGTTCT CGCCTGGATG TGGTTGAAAT GATGGACGGA CTGATGCAAG GCGCAGACCG CGACTTGGTT AAAGTATGGC AAAAACAAAA CGAATACCGT TTTGACAACA TTATGGTCAA CACCAAAACC GTTGCAGTCG AGCCGAAAGA AGACGGCGTT TACGTTACCT TTGAAGGTGC AAATGCACCT AAAGAGCCGC AACGTTACGA TGCCGTACTG GTCGCGGCAG GCCGTGCGCC TAACGGCAAA CTCATCAGCG CAGAAAAAGC CGGTGTTGCC GTTACCGATC GTGGCTTCAT CGAAGTTGAC AAACAAATGC GCACCAACGT ACCGCACATC TACGCTATCG GCGATATCGT CGGTCAACCG ATGTTGGCAC ACAAAGCCGT TCACGAAGGC CACGTTGCCG CTGAAAACTG TGCCGGCCAC AAAGCCTACT TCGACGCACG CGTTATTCCC GGCGTTGCCT ACACTTCCCC CGAAGTGGCA TGGGTCGGTG AAACCGAATT GTCTGCCAAA GCATCAGGCC GCAAAATCAC CAAAGCCAAC TTCCCATGGG CGGCTTCCGG TCGTGCGATT GCCAACGGTT GCGACAACGG CTTTACCAAG CTGATTTTCG ATGCCGAAAC CGGCCGCATC ATCGGCGGCG GCATTGTCGG TCCGAACGGT GGCGACATGA TCGGCGAAGT CTGCCTTGCC ATCGAAATGG GCTGCGACGC GGCAGACATC GGCAAAACCA TCCACCCGCA CCCGACCTTG GGCGAATCCA TCGGTATGGC TGCGGAAGTG GCTTTGGGTG TATGTACCGA CCTGCCTCCT CAAAAGAAAA AATAA
Details for NegoA.00731.a.B1.GE39973
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 63kDa |
| OBSERVED MW: | 63kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 37 |
Validated AA Sequence
MAHHHHHHMA LVELKVPDIG GHENVDIIAV EVNVGDTIAV DDTLITLETD KATMDVPAEV AGVIKEVKVK VGDKISEGGL IVVVEAEGAA AAPKAEAAAA PAQEAPKAAA PAPQAAQFGG AADAEYDVVV LGGGPGGYSA AFAAADEGLK VAIVERYKTL GGVCLNVGCI PSKALLHNAA VIDEVRHLAA NGIKYPKPEL DIDMLRAYKD GVVSRLTGGL AGMAEKP
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA LVELKVPDIG GHENVDIIAV EVNVGDTIAV DDTLITLETD KATMDVPAEV AGVIKEVKVK VGDKISEGGL IVVVEAEGAA AAPKAEAAAA PAQEAPKAAA PAPQAAQFGG AADAEYDVVV LGGGPGGYSA AFAAADEGLK VAIVERYKTL GGVCLNVGCI PSKALLHNAA VIDEVRHLAA NGIKYPEPEL DIDMLRAYKD GVVSRLTGGL AGMAKSRKVD VIQGDGQFLD PHHLEVSLTA GDAYEQAAPT GEKKIVAFKN CIIAAGSRVT KLPFIPEDPR IIDSSGALAL KEVPGKLLII GGGIIGLEMG TVYSTLGSRL DVVEMMDGLM QGADRDLVKV WQKQNEYRFD NIMVNTKTVA VEPKEDGVYV TFEGANAPKE PQRYDAVLVA AGRAPNGKLI SAEKAGVAVT DRGFIEVDKQ MRTNVPHIYA IGDIVGQPML AHKAVHEGHV AAENCAGHKA YFDARVIPGV AYTSPEVAWV GETELSAKAS GRKITKANFP WAASGRAIAN GCDNGFTKLI FDAETGRIIG GGIVGPNGGD MIGEVCLAIE MGCDAADIGK TIHPHPTLGE SIGMAAEVAL GVCTDLPPQK KK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctt tagttgaatt gaaagtgccc gacattggcg gacacgaaaa tgtagatatt atcgcggttg aagtgaacgt tggcgacacc atcgccgttg acgacaccct gattaccttg gaaaccgaca aagcgacgat ggacgtgcct gccgaagttg cgggcgtgat caaagaagtg aaagtcaaag tcggcgacaa aatctccgaa ggcggtttga ttgtcgtcgt cgaagccgaa ggtgcggctg ccgcccctaa agccgaagcg gctgccgccc cggcgcaaga agcacccaaa gctgccgctc ctgctccgca agccgcgcaa ttcggcggtg ctgccgatgc cgagtacgac gtggtcgtat tgggcggcgg tcccggcggt tactccgctg catttgccgc tgccgatgaa ggcttgaaag tcgccatcgt cgagcgttac aaaactttgg gcggcgtttg cctgaacgtc ggctgtatcc cttccaaagc cttgttgcac aatgccgccg ttatcgacga agtgcgccac ttggctgcca acggtatcaa ataccccgaa ccggaactcg acatcgatat gcttcgcgcc tacaaagacg gcgtggtttc ccgcctcacg ggtggtttgg caggtatggc gaaaagccgt aaagtggacg ttatccaagg cgacgggcaa ttcttggatc ctcaccactt ggaagtgtcg ctgactgccg gcgacgcgta cgaacaggca gcccctaccg gcgagaaaaa aatcgttgcc ttcaaaaact gtatcattgc agcaggcagc cgcgtaacca aactgccttt cattcctgaa gatccgcgca tcatcgattc cagcggcgca ctggcactga aagaagtacc gggcaaactg ctgattatcg gcggcggcat tatcggcctc gagatgggta cggtttacag cacgctgggt tctcgcctgg atgtggttga aatgatggac ggactgatgc aaggcgcaga ccgcgacttg gttaaagtat ggcaaaaaca aaacgaatac cgttttgaca acattatggt caacaccaaa accgttgcag tcgagccgaa agaagacggc gtttacgtta cctttgaagg tgcaaatgca cctaaagagc cgcaacgtta cgatgccgta ctggtcgcgg caggccgtgc gcctaacggc aaactcatca gcgcagaaaa agccggtgtt gccgttaccg atcgtggctt catcgaagtt gacaaacaaa tgcgcaccaa cgtaccgcac atctacgcta tcggcgatat cgtcggtcaa ccgatgttgg cacacaaagc cgttcacgaa ggccacgttg ccgctgaaaa ctgtgccggc cacaaagcct acttcgacgc acgcgttatt cccggcgttg cctacacttc ccccgaagtg gcatgggtcg gtgaaaccga attgtctgcc aaagcatcag gccgcaaaat caccaaagcc aacttcccat gggcggcttc cggtcgtgcg attgccaacg gttgcgacaa cggctttacc aagctgattt tcgatgccga aaccggccgc atcatcggcg gcggcattgt cggtccgaac ggtggcgaca tgatcggcga agtctgcctt gccatcgaaa tgggctgcga cgcggcagac atcggcaaaa ccatccaccc gcacccgacc ttgggcgaat ccatcggtat ggctgcggaa gtggctttgg gtgtatgtac cgacctgcct cctcaaaaga aaaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for NegoA.00731.a.B1.PW37934
| PURIFICATION DATe: | 8/1/2016 |
| CONCENTRATION: | 22mg/ml |
| OBSERVED MW: | 68kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 37 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA LVELKVPDIG GHENVDIIAV EVNVGDTIAV DDTLITLETD KATMDVPAEV AGVIKEVKVK VGDKISEGGL IVVVEAEGAA AAPKAEAAAA PAQEAPKAAA PAPQAAQFGG AADAEYDVVV LGGGPGGYSA AFAAADEGLK VAIVERYKTL GGVCLNVGCI PSKALLHNAA VIDEVRHLAA NGIKYPKPEL DIDMLRAYKD GVVSRLTGGL AGMAEKP
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA LVELKVPDIG GHENVDIIAV EVNVGDTIAV DDTLITLETD KATMDVPAEV AGVIKEVKVK VGDKISEGGL IVVVEAEGAA AAPKAEAAAA PAQEAPKAAA PAPQAAQFGG AADAEYDVVV LGGGPGGYSA AFAAADEGLK VAIVERYKTL GGVCLNVGCI PSKALLHNAA VIDEVRHLAA NGIKYPEPEL DIDMLRAYKD GVVSRLTGGL AGMAKSRKVD VIQGDGQFLD PHHLEVSLTA GDAYEQAAPT GEKKIVAFKN CIIAAGSRVT KLPFIPEDPR IIDSSGALAL KEVPGKLLII GGGIIGLEMG TVYSTLGSRL DVVEMMDGLM QGADRDLVKV WQKQNEYRFD NIMVNTKTVA VEPKEDGVYV TFEGANAPKE PQRYDAVLVA AGRAPNGKLI SAEKAGVAVT DRGFIEVDKQ MRTNVPHIYA IGDIVGQPML AHKAVHEGHV AAENCAGHKA YFDARVIPGV AYTSPEVAWV GETELSAKAS GRKITKANFP WAASGRAIAN GCDNGFTKLI FDAETGRIIG GGIVGPNGGD MIGEVCLAIE MGCDAADIGK TIHPHPTLGE SIGMAAEVAL GVCTDLPPQK KK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTT TAGTTGAATT GAAAGTGCCC GACATTGGCG GACACGAAAA TGTAGATATT ATCGCGGTTG AAGTGAACGT TGGCGACACC ATCGCCGTTG ACGACACCCT GATTACCTTG GAAACCGACA AAGCGACGAT GGACGTGCCT GCCGAAGTTG CGGGCGTGAT CAAAGAAGTG AAAGTCAAAG TCGGCGACAA AATCTCCGAA GGCGGTTTGA TTGTCGTCGT CGAAGCCGAA GGTGCGGCTG CCGCCCCTAA AGCCGAAGCG GCTGCCGCCC CGGCGCAAGA AGCACCCAAA GCTGCCGCTC CTGCTCCGCA AGCCGCGCAA TTCGGCGGTG CTGCCGATGC CGAGTACGAC GTGGTCGTAT TGGGCGGCGG TCCCGGCGGT TACTCCGCTG CATTTGCCGC TGCCGATGAA GGCTTGAAAG TCGCCATCGT CGAGCGTTAC AAAACTTTGG GCGGCGTTTG CCTGAACGTC GGCTGTATCC CTTCCAAAGC CTTGTTGCAC AATGCCGCCG TTATCGACGA AGTGCGCCAC TTGGCTGCCA ACGGTATCAA ATACCCCGAA CCGGAACTCG ACATCGATAT GCTTCGCGCC TACAAAGACG GCGTGGTTTC CCGCCTCACG GGTGGTTTGG CAGGTATGGC GAAAAGCCGT AAAGTGGACG TTATCCAAGG CGACGGGCAA TTCTTGGATC CTCACCACTT GGAAGTGTCG CTGACTGCCG GCGACGCGTA CGAACAGGCA GCCCCTACCG GCGAGAAAAA AATCGTTGCC TTCAAAAACT GTATCATTGC AGCAGGCAGC CGCGTAACCA AACTGCCTTT CATTCCTGAA GATCCGCGCA TCATCGATTC CAGCGGCGCA CTGGCACTGA AAGAAGTACC GGGCAAACTG CTGATTATCG GCGGCGGCAT TATCGGCCTC GAGATGGGTA CGGTTTACAG CACGCTGGGT TCTCGCCTGG ATGTGGTTGA AATGATGGAC GGACTGATGC AAGGCGCAGA CCGCGACTTG GTTAAAGTAT GGCAAAAACA AAACGAATAC CGTTTTGACA ACATTATGGT CAACACCAAA ACCGTTGCAG TCGAGCCGAA AGAAGACGGC GTTTACGTTA CCTTTGAAGG TGCAAATGCA CCTAAAGAGC CGCAACGTTA CGATGCCGTA CTGGTCGCGG CAGGCCGTGC GCCTAACGGC AAACTCATCA GCGCAGAAAA AGCCGGTGTT GCCGTTACCG ATCGTGGCTT CATCGAAGTT GACAAACAAA TGCGCACCAA CGTACCGCAC ATCTACGCTA TCGGCGATAT CGTCGGTCAA CCGATGTTGG CACACAAAGC CGTTCACGAA GGCCACGTTG CCGCTGAAAA CTGTGCCGGC CACAAAGCCT ACTTCGACGC ACGCGTTATT CCCGGCGTTG CCTACACTTC CCCCGAAGTG GCATGGGTCG GTGAAACCGA ATTGTCTGCC AAAGCATCAG GCCGCAAAAT CACCAAAGCC AACTTCCCAT GGGCGGCTTC CGGTCGTGCG ATTGCCAACG GTTGCGACAA CGGCTTTACC AAGCTGATTT TCGATGCCGA AACCGGCCGC ATCATCGGCG GCGGCATTGT CGGTCCGAAC GGTGGCGACA TGATCGGCGA AGTCTGCCTT GCCATCGAAA TGGGCTGCGA CGCGGCAGAC ATCGGCAAAA CCATCCACCC GCACCCGACC TTGGGCGAAT CCATCGGTAT GGCTGCGGAA GTGGCTTTGG GTGTATGTAC CGACCTGCCT CCTCAAAAGA AAAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT