NegoA.00617.a
glyceraldehyde-3-phosphate dehydrogenase
| CENTER ID: | NegoA.00617.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00617.a.B1.GE39971 | full length | 1 | 334 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00617.a.B1.PS38018 | full length | 1 | 334 |
Structures
5VMT
DEPOSITED: 4/28/2017
DETERMINATION: XRay
CLONE: NegoA.00617.a.B1.GE39971
PROTEIN: NegoA.00617.a.B1.PS38018
Publications by SSGCID
Structures of glyceraldehyde 3-phosphate dehydrogenase in Neisseria gonorrhoeae and Chlamydia trachomatis
Abendroth J, Barrett LK, Choi R, Dranow DM, Edwards TE, Fan E, Lorimer DD, Myler P, Phan I, Staker B, Subramanian S, Van Voorhis WC, Zhang Z, Craig JC, Ojo KK, Horanyi PS, Barrett KF, Shaheen S, Hybiske K, Conrady DG, Navaluna ED, Michaels SA, Soge OO, Tillery L
Protein Sci. - 2020
volume 29, issue 3, pages 768-778
PMID: 31930578; PMCID: PMC7020975
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.2495 |
| UniProt: | B4RPP8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIKVAINGF GRIGRLALRQ IEKAHGIEVA AVNDLTPAEM LLHLFKYDST QGRFQGTAEL KDDAIVVNGR EIKVFANPNP EELPWGELGV DVVLECTGFF TNKTKAEAHI RAGARKVVIS APGGNDVKTV VYGVNQDILD GSETVISAAS CTTNCLAPMA AVLQKEFGVV EGLMTTIHAY TGDQNTLDAP HRKGDLRRAR AAALNIVPNS TGAAKAIGLV IPELNGKLDG SAQRVPVATG SLTELVSVLE RPATKEEINA AMKAASSESY GYNEDQIVSS DVVGIEYGSL FDATQTRVMT VGGKQLVKTV AWYDNEMSYT CQLVRTLEYF AGKI
NT Sequence
ATGAGCATCA AAGTAGCGAT TAACGGTTTC GGCCGCATCG GCCGCCTCGC ATTGCGTCAA ATTGAAAAAG CCCACGGCAT CGAAGTCGCC GCCGTCAACG ACCTGACCCC TGCCGAAATG CTGCTGCACC TTTTCAAATA CGACAGCACA CAAGGCCGTT TCCAAGGTAC TGCCGAATTA AAAGACGATG CCATCGTGGT AAACGGCAGA GAAATCAAAG TTTTCGCCAA TCCGAATCCC GAAGAATTGC CTTGGGGCGA GTTGGGTGTG GATGTCGTCC TCGAATGTAC CGGTTTCTTT ACCAACAAAA CCAAAGCCGA AGCACATATC CGTGCCGGCG CGCGCAAAGT CGTGATTTCC GCACCCGGCG GCAATGATGT GAAAACCGTC GTATATGGCG TAAACCAAGA CATCTTGGAC GGTAGCGAAA CTGTCATTTC CGCCGCTTCC TGTACCACCA ACTGCCTTGC CCCGATGGCG GCAGTCCTGC AAAAAGAGTT CGGCGTAGTC GAAGGCCTGA TGACCACCAT CCACGCCTAC ACCGGCGACC AAAACACCCT TGACGCGCCG CACCGCAAAG GCGATTTGCG CCGCGCCCGC GCCGCCGCGC TCAACATCGT GCCCAACAGC ACCGGCGCCG CCAAAGCCAT CGGTCTGGTT ATCCCCGAAT TGAACGGCAA ACTCGACGGC TCCGCCCAAC GCGTCCCTGT TGCCACCGGC TCGCTGACCG AATTGGTTTC CGTCCTCGAA CGCCCTGCAA CCAAAGAAGA AATCAACGCC GCGATGAAAG CCGCCTCCAG CGAATCTTAC GGCTACAACG AAGATCAAAT CGTTTCTTCC GACGTTGTCG GCATCGAATA CGGCTCGCTC TTTGACGCAA CCCAAACCCG CGTGATGACC GTGGGCGGCA AACAACTGGT GAAAACCGTT GCCTGGTACG ACAACGAAAT GTCCTACACC TGCCAACTCG TCCGTACTTT GGAATACTTC GCAGGCAAAA TCTAA
Details for NegoA.00617.a.B1.GE39971
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS IKVAINGFGR IGRLALRQIE KAHGIEVAAV NDLTPAEMLL HLFKYDSTQG RFQGTAELKD DAIVVNGREI KVFANPNPEE LPWGELGVDV VLECTGFFTN KTKAEAHIRA GARKVVISAP GGNDVKTVVY GVNQDILDGS ETVISAASCT TNCLAPMAAV LQKEFGVVEG LMTTIHAYTG DQNTLDAPHR KGDLRRARAA ALNIVPNSTG AAKAIGLVIP ELNGKLDGSA QRVPVATGSL TELVSVLERP ATKEEINAAM KAASSESYGY NEDQIVSSDV VGIEYGSLFD ATQTRVMTVG GKQLVKTVAW YDNEMSYTCQ LV
Validated NT Sequence
acggacgagt tggcaggtgt aggacatttc gttgtcgtac caggcaacgg ttttcaccag ttgtttgccg cccacggtca tcacgcgggt ttgggttgcg tcaaagagcg agccgtattc gatgccgaca acgtcggaag aaacgatttg atcttcgttg tagccgtaag attcgctgga ggcggctttc atcgcggcgt tgatttcttc tttggttgca gggcgttcga ggacggaaac caattcggtc agcgagccgg tggcaacagg gacgcgttgg gcggagccgt cgagtttgcc gttcaattcg gggataacca gaccgatggc tttggcggcg ccggtgctgt tgggcacgat gttgagcgcg gcggcgcggg cgcggcgcaa atcgcctttg cggtgcggcg cgtcaagggt gttttggtcg ccggtgtagg cgtggatggt ggtcatcagg ccttcgacta cgccgaactc tttttgcagg actgccgcca tcggggcaag gcagttggtg gtacaggaag cggcggaaat gacagtttcg ctaccgtcca agatgtcttg gtttacgcca tatacgacgg ttttcacatc attgccgccg ggtgcagaaa tcacgacttt gcgcgcgccg gcacggatat gtgcttcggc tttggttttg ttggtaaaga aaccggtaca ttcgaggacg acatccacac ccaactcgcc ccaaggcaat tcttcgggat tcggattggc gaaaactttg atttctctgc cgtttaccac gatggcatcg tcttttaatt cggcagtacc ttggaaacgg ccttgtgtgc tgtcgtattt gaaaaggtgc agcagcattt cggcaggggt caggtcgttg acggcggcga cttcgatgcc gtgggctttt tcaatttgac gcaatgcgag gcggccgatg cggccgaaac cgttaatcgc tactttgatg ctcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMS IKVAINGFGR IGRLALRQIE KAHGIEVAAV NDLTPAEMLL HLFKYDSTQG RFQGTAELKD DAIVVNGREI KVFANPNPEE LPWGELGVDV VLECTGFFTN KTKAEAHIRA GARKVVISAP GGNDVKTVVY GVNQDILDGS ETVISAASCT TNCLAPMAAV LQKEFGVVEG LMTTIHAYTG DQNTLDAPHR KGDLRRARAA ALNIVPNSTG AAKAIGLVIP ELNGKLDGSA QRVPVATGSL TELVSVLERP ATKEEINAAM KAASSESYGY NEDQIVSSDV VGIEYGSLFD ATQTRVMTVG GKQLVKTVAW YDNEMSYTCQ LVRTLEYFAG KI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca tcaaagtagc gattaacggt ttcggccgca tcggccgcct cgcattgcgt caaattgaaa aagcccacgg catcgaagtc gccgccgtca acgacctgac ccctgccgaa atgctgctgc accttttcaa atacgacagc acacaaggcc gtttccaagg tactgccgaa ttaaaagacg atgccatcgt ggtaaacggc agagaaatca aagttttcgc caatccgaat cccgaagaat tgccttgggg cgagttgggt gtggatgtcg tcctcgaatg taccggtttc tttaccaaca aaaccaaagc cgaagcacat atccgtgccg gcgcgcgcaa agtcgtgatt tccgcacccg gcggcaatga tgtgaaaacc gtcgtatatg gcgtaaacca agacatcttg gacggtagcg aaactgtcat ttccgccgct tcctgtacca ccaactgcct tgccccgatg gcggcagtcc tgcaaaaaga gttcggcgta gtcgaaggcc tgatgaccac catccacgcc tacaccggcg accaaaacac ccttgacgcg ccgcaccgca aaggcgattt gcgccgcgcc cgcgccgccg cgctcaacat cgtgcccaac agcaccggcg ccgccaaagc catcggtctg gttatccccg aattgaacgg caaactcgac ggctccgccc aacgcgtccc tgttgccacc ggctcgctga ccgaattggt ttccgtcctc gaacgccctg caaccaaaga agaaatcaac gccgcgatga aagccgcctc cagcgaatct tacggctaca acgaagatca aatcgtttct tccgacgttg tcggcatcga atacggctcg ctctttgacg caacccaaac ccgcgtgatg accgtgggcg gcaaacaact ggtgaaaacc gttgcctggt acgacaacga aatgtcctac acctgccaac tcgtccgtac tttggaatac ttcgcaggca aaatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for NegoA.00617.a.B1.PS38018
| PURIFICATION DATe: | 8/17/2016 |
| CONCENTRATION: | 32.15mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS IKVAINGFGR IGRLALRQIE KAHGIEVAAV NDLTPAEMLL HLFKYDSTQG RFQGTAELKD DAIVVNGREI KVFANPNPEE LPWGELGVDV VLECTGFFTN KTKAEAHIRA GARKVVISAP GGNDVKTVVY GVNQDILDGS ETVISAASCT TNCLAPMAAV LQKEFGVVEG LMTTIHAYTG DQNTLDAPHR KGDLRRARAA ALNIVPNSTG AAKAIGLVIP ELNGKLDGSA QRVPVATGSL TELVSVLERP ATKEEINAAM KAASSESYGY NEDQIVSSDV VGIEYGSLFD ATQTRVMTVG GKQLVKTVAW YDNEMSYTCQ LV
Validated NT Sequence
acggacgagt tggcaggtgt aggacatttc gttgtcgtac caggcaacgg ttttcaccag ttgtttgccg cccacggtca tcacgcgggt ttgggttgcg tcaaagagcg agccgtattc gatgccgaca acgtcggaag aaacgatttg atcttcgttg tagccgtaag attcgctgga ggcggctttc atcgcggcgt tgatttcttc tttggttgca gggcgttcga ggacggaaac caattcggtc agcgagccgg tggcaacagg gacgcgttgg gcggagccgt cgagtttgcc gttcaattcg gggataacca gaccgatggc tttggcggcg ccggtgctgt tgggcacgat gttgagcgcg gcggcgcggg cgcggcgcaa atcgcctttg cggtgcggcg cgtcaagggt gttttggtcg ccggtgtagg cgtggatggt ggtcatcagg ccttcgacta cgccgaactc tttttgcagg actgccgcca tcggggcaag gcagttggtg gtacaggaag cggcggaaat gacagtttcg ctaccgtcca agatgtcttg gtttacgcca tatacgacgg ttttcacatc attgccgccg ggtgcagaaa tcacgacttt gcgcgcgccg gcacggatat gtgcttcggc tttggttttg ttggtaaaga aaccggtaca ttcgaggacg acatccacac ccaactcgcc ccaaggcaat tcttcgggat tcggattggc gaaaactttg atttctctgc cgtttaccac gatggcatcg tcttttaatt cggcagtacc ttggaaacgg ccttgtgtgc tgtcgtattt gaaaaggtgc agcagcattt cggcaggggt caggtcgttg acggcggcga cttcgatgcc gtgggctttt tcaatttgac gcaatgcgag gcggccgatg cggccgaaac cgttaatcgc tactttgatg ctcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMS IKVAINGFGR IGRLALRQIE KAHGIEVAAV NDLTPAEMLL HLFKYDSTQG RFQGTAELKD DAIVVNGREI KVFANPNPEE LPWGELGVDV VLECTGFFTN KTKAEAHIRA GARKVVISAP GGNDVKTVVY GVNQDILDGS ETVISAASCT TNCLAPMAAV LQKEFGVVEG LMTTIHAYTG DQNTLDAPHR KGDLRRARAA ALNIVPNSTG AAKAIGLVIP ELNGKLDGSA QRVPVATGSL TELVSVLERP ATKEEINAAM KAASSESYGY NEDQIVSSDV VGIEYGSLFD ATQTRVMTVG GKQLVKTVAW YDNEMSYTCQ LVRTLEYFAG KI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA TCAAAGTAGC GATTAACGGT TTCGGCCGCA TCGGCCGCCT CGCATTGCGT CAAATTGAAA AAGCCCACGG CATCGAAGTC GCCGCCGTCA ACGACCTGAC CCCTGCCGAA ATGCTGCTGC ACCTTTTCAA ATACGACAGC ACACAAGGCC GTTTCCAAGG TACTGCCGAA TTAAAAGACG ATGCCATCGT GGTAAACGGC AGAGAAATCA AAGTTTTCGC CAATCCGAAT CCCGAAGAAT TGCCTTGGGG CGAGTTGGGT GTGGATGTCG TCCTCGAATG TACCGGTTTC TTTACCAACA AAACCAAAGC CGAAGCACAT ATCCGTGCCG GCGCGCGCAA AGTCGTGATT TCCGCACCCG GCGGCAATGA TGTGAAAACC GTCGTATATG GCGTAAACCA AGACATCTTG GACGGTAGCG AAACTGTCAT TTCCGCCGCT TCCTGTACCA CCAACTGCCT TGCCCCGATG GCGGCAGTCC TGCAAAAAGA GTTCGGCGTA GTCGAAGGCC TGATGACCAC CATCCACGCC TACACCGGCG ACCAAAACAC CCTTGACGCG CCGCACCGCA AAGGCGATTT GCGCCGCGCC CGCGCCGCCG CGCTCAACAT CGTGCCCAAC AGCACCGGCG CCGCCAAAGC CATCGGTCTG GTTATCCCCG AATTGAACGG CAAACTCGAC GGCTCCGCCC AACGCGTCCC TGTTGCCACC GGCTCGCTGA CCGAATTGGT TTCCGTCCTC GAACGCCCTG CAACCAAAGA AGAAATCAAC GCCGCGATGA AAGCCGCCTC CAGCGAATCT TACGGCTACA ACGAAGATCA AATCGTTTCT TCCGACGTTG TCGGCATCGA ATACGGCTCG CTCTTTGACG CAACCCAAAC CCGCGTGATG ACCGTGGGCG GCAAACAACT GGTGAAAACC GTTGCCTGGT ACGACAACGA AATGTCCTAC ACCTGCCAAC TCGTCCGTAC TTTGGAATAC TTCGCAGGCA AAATCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT