| CENTER ID: | NegoA.00612.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | native diffraction-data |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00612.a.A11.GE43342 | full length | 1 | 503 | ||
| NegoA.00612.a.A110.GE43343 | full length | 17 | 503 | ||
| NegoA.00612.a.A12.GE43344 | full length | 1 | 503 | ||
| NegoA.00612.a.A14.GE43346 | full length | 5 | 503 | ||
| NegoA.00612.a.A15.GE43347 | full length | 7 | 503 | ||
| NegoA.00612.a.A17.GE43349 | full length | 11 | 503 | ||
| NegoA.00612.a.A18.GE43350 | full length | 13 | 503 | ||
| NegoA.00612.a.B1.GE39969 | full length | 1 | 503 |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00612.a.B1.PW37916 | full length | 1 | 503 | ||
| NegoA.00612.a.A11.PW38713 | full length | 1 | 503 | ||
| NegoA.00612.a.A110.PW38714 | full length | 17 | 503 | ||
| NegoA.00612.a.A12.PW38715 | full length | 1 | 503 | ||
| NegoA.00612.a.A14.PW38716 | full length | 5 | 503 | ||
| NegoA.00612.a.A15.PW38717 | full length | 7 | 503 | ||
| NegoA.00612.a.A18.PW38718 | full length | 13 | 503 | ||
| NegoA.00612.a.A17.PW38753 | full length | 11 | 503 |
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_85037 |
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|521006.8.peg.1828 |
| UniProt: | B4RP57 |
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 60kDa |
| OBSERVED MW: | 60kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 56 |
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 60kDa |
| OBSERVED MW: | 60kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 59kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 56 |
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 59kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 56 |
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 55 |
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 73 |
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 70 |
| PERCENT COVERAGE: | 98 |
| PURIFICATION DATe: | 6/7/2016 |
| CONCENTRATION: | 31mg/ml |
| OBSERVED MW: | 60kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 19 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 70 |
| PERCENT COVERAGE: | 98 |
| PURIFICATION DATe: | 10/8/2019 |
| CONCENTRATION: | 45mg/ml |
| OBSERVED MW: | 60kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 56 |
| PURIFICATION DATe: | 10/8/2019 |
| CONCENTRATION: | 38.7mg/ml |
| OBSERVED MW: | 58kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 19 |
| VIAL VOLUME: | 25µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 10/9/2019 |
| CONCENTRATION: | 45.5mg/ml |
| OBSERVED MW: | 60kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| PURIFICATION DATe: | 10/9/2019 |
| CONCENTRATION: | 31.7mg/ml |
| OBSERVED MW: | 59kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 56 |
| PURIFICATION DATe: | 10/10/2019 |
| CONCENTRATION: | 39.8mg/ml |
| OBSERVED MW: | 59kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 56 |
| PURIFICATION DATe: | 10/10/2019 |
| CONCENTRATION: | 46.6mg/ml |
| OBSERVED MW: | 58kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 23µl |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 73 |
| PURIFICATION DATe: | 11/10/2019 |
| CONCENTRATION: | 36.7mg/ml |
| OBSERVED MW: | 58kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 55 |