NegoA.00612.a

lysyl-tRNA synthetase

CENTER ID:	NegoA.00612.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
NegoA.00612.a.A11.GE43342	full length	1	503
NegoA.00612.a.A110.GE43343	full length	17	503
NegoA.00612.a.A12.GE43344	full length	1	503
NegoA.00612.a.A14.GE43346	full length	5	503
NegoA.00612.a.A15.GE43347	full length	7	503
NegoA.00612.a.A17.GE43349	full length	11	503
NegoA.00612.a.A18.GE43350	full length	13	503
NegoA.00612.a.B1.GE39969	full length	1	503

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
NegoA.00612.a.B1.PW37916	full length	1	503
NegoA.00612.a.A11.PW38713	full length	1	503
NegoA.00612.a.A110.PW38714	full length	17	503
NegoA.00612.a.A12.PW38715	full length	1	503
NegoA.00612.a.A14.PW38716	full length	5	503
NegoA.00612.a.A15.PW38717	full length	7	503
NegoA.00612.a.A18.PW38718	full length	13	503
NegoA.00612.a.A17.PW38753	full length	11	503

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_85037

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.1828
UniProt:	B4RP57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEQNHPQTE PQLDENQIIA LRREKLNNIR QQRNAYPNDF KRDSFAADLQ AQYGEIGKEE LDPQAVPVKI AGRMMLKRQM GKASFATIQD VTGQIQLYLN NKGVSQEVLD DFNHWDLGDI VGAEGTLFKT NHGELTVRVS DIRLLSKSLR PLPDKHKGLS DQETKYRQRY VDLIANEESR NTFIKRSQII QSVRNFMVGE HYLEVETPMM HPIPGGATAK PFVTHHNALD IPLYLRIAPE LYLKRLVVGG LERVFEINRS FRNEGMSVRH NPEFTMIEFY EAFSDYERMM QMAEDIIRNA SRTVNGTANI TYNGKEVDLE SPFERLTILE AIKKYNPHYT DEQLNDAEWL KKEIVKHGES LPPSPGIGSL QLALFEGCAE GKLWNPTFIV DYPVEVSPLA RASDTKQGLT ERFELFVVGR ELANGYSELN DPEDQAERFK SQVAQKDAGD DEAMHYDADY IRAMEFGLPP TGGCGIGIDR LVMLLTDLQT IRDVILFPQM RPE

NT Sequence

ATGAGCGAAC AAAACCATCC GCAAACCGAG CCGCAGTTGG ACGAAAACCA AATCATCGCC CTGCGCCGCG AAAAACTGAA CAACATCCGC CAACAGCGCA ACGCTTACCC CAACGATTTC AAGCGCGACA GCTTTGCCGC CGATTTGCAG GCGCAATACG GCGAAATCGG CAAAGAAGAA CTCGACCCGC AGGCCGTTCC CGTTAAAATT GCCGGCCGCA TGATGCTCAA ACGTCAAATG GGCAAAGCCA GCTTCGCCAC CATTCAAGAC GTTACCGGTC AAATCCAGCT TTATCTGAAC AACAAAGGCG TGAGCCAAGA AGTTTTGGAC GACTTCAACC ACTGGGACTT GGGCGACATC GTCGGTGCGG AAGGCACTTT GTTCAAAACC AACCACGGCG AACTAACCGT GCGTGTGTCC GACATCCGCC TGCTGTCCAA ATCCCTGCGT CCGCTGCCCG ACAAACACAA AGGCTTGAGC GATCAGGAAA CCAAATACCG CCAACGCTAC GTCGATTTGA TTGCCAATGA AGAATCGCGC AATACCTTTA TCAAACGCAG CCAAATCATC CAATCTGTGC GTAATTTTAT GGTGGGCGAG CATTATCTCG AAGTCGAAAC CCCGATGATG CACCCGATTC CCGGCGGCGC GACAGCGAAA CCCTTCGTTA CCCACCACAA TGCCTTGGAC ATTCCGCTCT ACCTGCGCAT CGCGCCCGAG CTGTATCTGA AACGCTTGGT GGTCGGCGGC TTGGAACGCG TGTTTGAAAT CAACCGCAGC TTCCGTAACG AGGGCATGTC CGTGCGCCAC AACCCCGAAT TTACGATGAT TGAATTTTAC GAAGCCTTCT CCGACTACGA ACGTATGATG CAGATGGCGG AAGACATCAT CCGCAACGCA TCGCGCACGG TAAACGGCAC GGCAAACATC ACTTACAACG GCAAAGAAGT CGATTTGGAA AGCCCGTTCG AACGTCTGAC CATCCTCGAA GCCATCAAAA AATACAATCC GCACTACACC GACGAGCAGT TGAACGATGC GGAATGGCTG AAAAAAGAAA TCGTCAAACA CGGCGAAAGC CTGCCGCCGT CCCCGGGCAT CGGCAGCCTG CAACTCGCCC TGTTTGAAGG TTGCGCCGAG GGCAAACTGT GGAATCCGAC CTTTATTGTC GATTACCCGG TCGAAGTTTC CCCGTTGGCG CGCGCTTCGG ATACGAAACA AGGTCTGACC GAACGCTTCG AGCTTTTCGT TGTCGGGCGC GAACTGGCAA ACGGCTATTC CGAGTTGAAC GACCCCGAAG ACCAAGCCGA ACGCTTCAAA TCGCAAGTGG CGCAAAAAGA TGCGGGCGAC GACGAAGCCA TGCACTACGA TGCCGACTAC ATCCGCGCGA TGGAATTCGG TTTGCCGCCG ACCGGCGGTT GCGGCATCGG CATCGACCGC TTAGTGATGC TGCTGACCGA TTTGCAAACC ATCCGCGATG TGATTCTGTT TCCGCAGATG CGTCCCGAGT AA

Details for NegoA.00612.a.A11.GE43342

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	60kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	56

Validated AA Sequence

Validated NT Sequence

atatacgact cnctataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcga acaaaaccat ccgcaaaccg agccgcagtt ggacgaaaac caaatcatcg ccctgcgccg cgaaaaactg aacaacatcc gccaacagcg caacgcttat cccaacgatt tcaagcgcga cagctttgcc gccgatttgc aggcgcaata cggcgaaatc ggcaaagaag aactcgaccc gcaggccgtt cccgttaaaa ttgccggccg catgatgctc aaacgtcaaa tgggcaaagc cagcttcgcc accattcaag acgttaccgg tcaaatccag ctttatctga acaacaaagg cgtgagccaa gaagttttgg acgacttcaa ccactgggac ttgggcgaca tcgtcggtgc ggaaggcact ttgttcaaaa ccaaccacgg cgaactaacc gtgcgtgtgt ccgacatccg cctgctgtcc aaatccctgc gtccgctgcc cgacaaacac aaaggcttga gcgatcagga aaccaaatac cgccaacgct acgtcgattt gattgccaat gaagaatcgc gcaatacctt tatcaaacgc agccaaatca tccaatctgt gcgtaatttt atggtgggcg agcattatct cgaagtcgaa accccgatga tgcacccgat tcccggcggc gcgacagcga aacccttcgt tacccaccac aatgccttgg acattccgct ctacctgcgc atcgcgcccg anctgtatct gaaacgcttg gnggncggcg gcttggnacg cgtgtttgaa atcanccgca gcttccgtaa cgagggcatg tccgtgcgcn nnnncccgaa tttncgatga ttgnatttta cgaagccttc tccgactacg aacgtatgat gcagatggnn gangacatca tccgcaacnc atcnngncac ngtnannngn ncngnnaaac nnnnncttan nancggnnaa anaantcnat tnnnnnncnn ncgaancgtn nnnnnnncnt nnancnnnaa nnnncnnnnc cgnnctannn nnannnnnnn nnnancnatn nnnngnnnnn anncnnnnan nnnggnnnan nnnnngnnnn nnnncnccnn gnnnat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSEQNHPQT EPQLDENQII ALRREKLNNI RQQRNAYPND FKRDSFAADL QAQYGEIGKE ELDPQAVPVK IAGRMMLKRQ MGKASFATIQ DVTGQIQLYL NNKGVSQEVL DDFNHWDLGD IVGAEGTLFK TNHGELTVRV SDIRLLSKSL RPLPDKHKGL SDQETKYRQR YVDLIANEES RNTFIKRSQI IQSVRNFMVG EHYLEVETPM MHPIPGGATA KPFVTHHNAL DIPLYLRIAP ELYLKRLVVG GLERVFEINR SFRNEGMSVR HNPEFTMIEF YEAFSDYERM MQMAEDIIRN ASRTVNGTAN ITYNGKEVDL ESPFERLTIL EAIKKYNPHY TDEQLNDAEW LKKEIVKHGE SLPPSPGIGS LQLALFEGTA EGKLWNPTFI VDYPVEVSPL ARASDTKQGL TERFELFVVG RELANGYSEL NDPEDQAERF KSQVAQKDAG DDEAMHYDAD YIRAMEFGLP PTGGCGIGID RLVMLLTDLQ TIRDVILFPQ MRPE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgaacaa aaccatccgc aaaccgagcc gcagttggac gaaaaccaaa tcatcgccct gcgccgcgaa aaactgaaca acatccgcca acagcgcaac gcttacccca acgatttcaa gcgcgacagc tttgccgccg atttgcaggc gcaatacggc gaaatcggca aagaagaact cgacccgcag gccgttcccg ttaaaattgc cggccgcatg atgctcaaac gtcaaatggg caaagccagc ttcgccacca ttcaagacgt taccggtcaa atccagcttt atctgaacaa caaaggcgtg agccaagaag ttttggacga cttcaaccac tgggacttgg gcgacatcgt cggtgcggaa ggcactttgt tcaaaaccaa ccacggcgaa ctaaccgtgc gtgtgtccga catccgcctg ctgtccaaat ccctgcgtcc gctgcccgac aaacacaaag gcttgagcga tcaggaaacc aaataccgcc aacgctacgt cgatttgatt gccaatgaag aatcgcgcaa tacctttatc aaacgcagcc aaatcatcca atctgtgcgt aattttatgg tgggcgagca ttatctcgaa gtcgaaaccc cgatgatgca cccgattccc ggcggcgcga cagcgaaacc cttcgttacc caccacaatg ccttggacat tccgctctac ctgcgcatcg cgcccgagct gtatctgaaa cgcttggtgg tcggcggctt ggaacgcgtg tttgaaatca accgcagctt ccgtaacgag ggcatgtccg tgcgccacaa ccccgaattt acgatgattg aattttacga agccttctcc gactacgaac gtatgatgca gatggcggaa gacatcatcc gcaacgcatc gcgcacggta aacggcacgg caaacatcac ttacaacggc aaagaagtcg atttggaaag cccgttcgaa cgtctgacca tcctcgaagc catcaaaaaa tacaatccgc actacaccga cgagcagttg aacgatgcgg aatggctgaa aaaagaaatc gtcaaacacg gcgaaagcct gccgccgtcc ccgggcatcg gcagcctgca actcgccctg tttgaaggta ccgccgaggg caaactgtgg aatccgacct ttattgtcga ttacccggtc gaagtttccc cgttggcgcg cgcttcggat acgaaacaag gtctgaccga acgcttcgag cttttcgttg tcgggcgcga actggcaaac ggctattccg agttgaacga ccccgaagac caagccgaac gcttcaaatc gcaagtggcg caaaaagatg cgggcgacga cgaagccatg cactacgatg ccgactacat ccgcgcgatg gaattcggtt tgccgccgac cggcggttgc ggcatcggca tcgaccgctt agtgatgctg ctgaccgatt tgcaaaccat ccgcgatgtg attctgtttc cgcagatgcg tcccgagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for NegoA.00612.a.A110.GE43343

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMQIIALRRE KLNNIRQQRN AYPNDFKRDS FAADLQAQYG EIGKEELDPQ AVPVKIAGRM MLKRQMGKAS FATIQDVTGQ IQLYLNNKGV SQEVLDDFNH WDLGDIVGAE GTLFKTNHGE LTVRVSDIRL LSKSLRPLPD KHKGLSDQET KYRQRYVDLI ANEESRNTFI KRSQIIQSVR NFMVGEHYLE VETPMMHPIP GGATAKPFVT HHNALDIPLY LRIAPELYLK RLVVGGLERV FEINRSFRNE GMSVRHNPEF TMIEFYEAFS DYERMMQMAE DXIRNASRTV NGTANITYNG KEVDLESPFE RLTILEAIKK YNPHYTDEQL NDAEWLAAEI VKHGESLPPS PGIGSLQLAL FEGTAEGKLW NPTFIVDYPV EVSPLARASD TKQGLTERFE LFVVGRELAN GYSELNDPED QAERFKSQVA QKDAGDDEAM HYDADYIRAM EFGLPPTGGC GIGIDRLVML LTDLQTIRDV ILFPQMRPXX

Validated NT Sequence

tnttnntngg gacgcatctg cggaaacaga atcacatcgc ggatggtttg caaatcggtc agcagcatca ctaagcggtc gatgccgatg ccgcaaccgc cggtcggcgg caaaccgaat tccatcgcgc ggatgtagtc ggcatcgtag tgcatggctt cgtcgtcgcc cgcatctttt tgcgccactt gcgatttgaa gcgttcggct tggtcttcgg ggtcgttcaa ctcggaatag ccgtttgcca gttcgcgccc gacaacgaaa agctcgaagc gttcggtcag accttgtttc gtatccgaag cgcgcgccaa cggggaaact tcgaccgggt aatcgacaat aaaggtcgga ttccacagtt tgccctcggc ggtaccttca aacagggcga gttgcaggct gccgatgccc ggggacggcg gcaggctttc gccgtgtttg acgatttcag ctgccagcca ttccgcatcg ttcaactgct cgtcggtgta gtgcggattg tattttttga tggcttcgag gatggtcaga cgttcgaacg ggctttccaa atcgacttct ttgccgttgt aagtgatgtt tgccgtgccg tttaccgtgc gcgatgcgtt gcggatgang tcttccgcca tctgcatcat acgttcgtag tcggagaagg cttcgtaaaa ttcaatcatc gtaaattcgg ggttgtggcg cacggacatg ccctcgttac ggaagctgcg gttgatttca aacacgcgtt ccaagccgcc gaccaccaag cgtttcagat acagctcggg cgcgatgcgc aggtagagcg gaatgtccaa ggcattgtgg tgggtaacga agggtttcgc tgtcgcgccg ccgggaatcg ggtgcatcat cggggtttcg acttcgagat aatgctcgcc caccataaaa ttacgcacag attggatgat ttggctgcgt ttgataaagg tattgcgcga ttcttcattg gcaatcaaat cgacgtagcg ttggcggtat ttggtttcct gatcgctcaa gcctttgtgt ttgtcgggca gcggacgcag ggatttggac agcaggcgga tgtcggacac acgcacggtt agttcgccgt ggttggtttt gaacaaagtg ccttccgcac cgacgatgtc gcccaagtcc cagtggttga agtcgtccaa aacttcttgg ctcacgcctt tgttgttcag ataaagctgg atttgaccgg taacgtcttg aatggtggcg aagctggctt tgcccatttg acgtttgagc atcatgcggc cggcaatttt aacgggaacg gcctgcgggt cgagttcttc tttgccgatt tcgccgtatt gcgcctgcaa atcggcggca aagctgtcgc gcttgaaatc gttgggataa gcgttgcgct gttggcggat gttgttcagt ttttcgcggc gcagggcgat gatttgcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SQIIALRREK LNNIRQQRNA YPNDFKRDSF AADLQAQYGE IGKEELDPQA VPVKIAGRMM LKRQMGKASF ATIQDVTGQI QLYLNNKGVS QEVLDDFNHW DLGDIVGAEG TLFKTNHGEL TVRVSDIRLL SKSLRPLPDK HKGLSDQETK YRQRYVDLIA NEESRNTFIK RSQIIQSVRN FMVGEHYLEV ETPMMHPIPG GATAKPFVTH HNALDIPLYL RIAPELYLKR LVVGGLERVF EINRSFRNEG MSVRHNPEFT MIEFYEAFSD YERMMQMAED IIRNASRTVN GTANITYNGK EVDLESPFER LTILEAIKKY NPHYTDEQLN DAEWLAAEIV KHGESLPPSP GIGSLQLALF EGTAEGKLWN PTFIVDYPVE VSPLARASDT KQGLTERFEL FVVGRELANG YSELNDPEDQ AERFKSQVAQ KDAGDDEAMH YDADYIRAME FGLPPTGGCG IGIDRLVMLL TDLQTIRDVI LFPQMRPE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgca aatcatcgcc ctgcgccgcg aaaaactgaa caacatccgc caacagcgca acgcttaccc caacgatttc aagcgcgaca gctttgccgc cgatttgcag gcgcaatacg gcgaaatcgg caaagaagaa ctcgacccgc aggccgttcc cgttaaaatt gccggccgca tgatgctcaa acgtcaaatg ggcaaagcca gcttcgccac cattcaagac gttaccggtc aaatccagct ttatctgaac aacaaaggcg tgagccaaga agttttggac gacttcaacc actgggactt gggcgacatc gtcggtgcgg aaggcacttt gttcaaaacc aaccacggcg aactaaccgt gcgtgtgtcc gacatccgcc tgctgtccaa atccctgcgt ccgctgcccg acaaacacaa aggcttgagc gatcaggaaa ccaaataccg ccaacgctac gtcgatttga ttgccaatga agaatcgcgc aataccttta tcaaacgcag ccaaatcatc caatctgtgc gtaattttat ggtgggcgag cattatctcg aagtcgaaac cccgatgatg cacccgattc ccggcggcgc gacagcgaaa cccttcgtta cccaccacaa tgccttggac attccgctct acctgcgcat cgcgcccgag ctgtatctga aacgcttggt ggtcggcggc ttggaacgcg tgtttgaaat caaccgcagc ttccgtaacg agggcatgtc cgtgcgccac aaccccgaat ttacgatgat tgaattttac gaagccttct ccgactacga acgtatgatg cagatggcgg aagacatcat ccgcaacgca tcgcgcacgg taaacggcac ggcaaacatc acttacaacg gcaaagaagt cgatttggaa agcccgttcg aacgtctgac catcctcgaa gccatcaaaa aatacaatcc gcactacacc gacgagcagt tgaacgatgc ggaatggctg gcagctgaaa tcgtcaaaca cggcgaaagc ctgccgccgt ccccgggcat cggcagcctg caactcgccc tgtttgaagg taccgccgag ggcaaactgt ggaatccgac ctttattgtc gattacccgg tcgaagtttc cccgttggcg cgcgcttcgg atacgaaaca aggtctgacc gaacgcttcg agcttttcgt tgtcgggcgc gaactggcaa acggctattc cgagttgaac gaccccgaag accaagccga acgcttcaaa tcgcaagtgg cgcaaaaaga tgcgggcgac gacgaagcca tgcactacga tgccgactac atccgcgcga tggaattcgg tttgccgccg accggcggtt gcggcatcgg catcgaccgc ttagtgatgc tgctgaccga tttgcaaacc atccgcgatg tgattctgtt tccgcagatg cgtcccgagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for NegoA.00612.a.A12.GE43344

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	60kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSEQNHPQT EPQLDENQII ALRREKLNNI RQQRNAYPND FKRDSFAADL QAQYGEIGKE ELDPQAVPVK IAGRMMLKRQ MGKASFATIQ DVTGQIQLYL NNKGVSQEVL DDFNHWDLGD IVGAEGTLFK TNHGELTVRV SDIRLLSKSL RPLPDKHKGL SDQETKYRQR YVDLIANEES RNTFIKRSQI IQSVRNFMVG EHYLEVETPM MHPIPGGATA KPFVTHHNAL DIPLYLRIAP ELYLKRLVVG GLERVFEINR SFRNEGMSVR HNPEFTMIEF YEAFSDYERM MQMAEDIIRN ASRTVNGTAN ITYNGKEVDL ESPFERLTIL EAIKKYNPHY TDEQLNDAEW LAAEIVKHGE SLPPSPGIGS LQLALFEGTA EGKLWNPTFI VDYPVEVSPL ARASDTKQGL TERFELFVVG RELANGYSEL NDPEDQAERF KSQVAQKDAG DDEAMHYDAD YIRAMEFGLP PTGGCGIGID RLVMLLTDLQ TIRDVILFPQ MRPE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgaacaa aaccatccgc aaaccgagcc gcagttggac gaaaaccaaa tcatcgccct gcgccgcgaa aaactgaaca acatccgcca acagcgcaac gcttacccca acgatttcaa gcgcgacagc tttgccgccg atttgcaggc gcaatacggc gaaatcggca aagaagaact cgacccgcag gccgttcccg ttaaaattgc cggccgcatg atgctcaaac gtcaaatggg caaagccagc ttcgccacca ttcaagacgt taccggtcaa atccagcttt atctgaacaa caaaggcgtg agccaagaag ttttggacga cttcaaccac tgggacttgg gcgacatcgt cggtgcggaa ggcactttgt tcaaaaccaa ccacggcgaa ctaaccgtgc gtgtgtccga catccgcctg ctgtccaaat ccctgcgtcc gctgcccgac aaacacaaag gcttgagcga tcaggaaacc aaataccgcc aacgctacgt cgatttgatt gccaatgaag aatcgcgcaa tacctttatc aaacgcagcc aaatcatcca atctgtgcgt aattttatgg tgggcgagca ttatctcgaa gtcgaaaccc cgatgatgca cccgattccc ggcggcgcga cagcgaaacc cttcgttacc caccacaatg ccttggacat tccgctctac ctgcgcatcg cgcccgagct gtatctgaaa cgcttggtgg tcggcggctt ggaacgcgtg tttgaaatca accgcagctt ccgtaacgag ggcatgtccg tgcgccacaa ccccgaattt acgatgattg aattttacga agccttctcc gactacgaac gtatgatgca gatggcggaa gacatcatcc gcaacgcatc gcgcacggta aacggcacgg caaacatcac ttacaacggc aaagaagtcg atttggaaag cccgttcgaa cgtctgacca tcctcgaagc catcaaaaaa tacaatccgc actacaccga cgagcagttg aacgatgcgg aatggctggc agctgaaatc gtcaaacacg gcgaaagcct gccgccgtcc ccgggcatcg gcagcctgca actcgccctg tttgaaggta ccgccgaggg caaactgtgg aatccgacct ttattgtcga ttacccggtc gaagtttccc cgttggcgcg cgcttcggat acgaaacaag gtctgaccga acgcttcgag cttttcgttg tcgggcgcga actggcaaac ggctattccg agttgaacga ccccgaagac caagccgaac gcttcaaatc gcaagtggcg caaaaagatg cgggcgacga cgaagccatg cactacgatg ccgactacat ccgcgcgatg gaattcggtt tgccgccgac cggcggttgc ggcatcggca tcgaccgctt agtgatgctg ctgaccgatt tgcaaaccat ccgcgatgtg attctgtttc cgcagatgcg tcccgagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for NegoA.00612.a.A14.GE43346

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	59kDa
OBSERVED MW:	59kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	56

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNHPQTEPQ LDENQIIALR REKLNNIRQQ RNAYPNDFKR DSFAADLQAQ YGEIGKEELD PQAVPVKIAG RMMLKRQMGK ASFATIQDVT GQIQLYLNNK GVSQEVLDDF NHWDLGDIVG AEGTLFKTNH GELTVRVSDI RLLSKSLRPL PDKHKGLSDQ ETKYRQRYVD LIANEESRNT FIKRSQIIQS VRNFMVGEHY LEVETPMMHP IPGGATAKPF VTHHNALDIP LYLRIAPXLY LKRLVVGGLE RVXEINRSFR NEGMXVXHNP XIXDD

Validated NT Sequence

atatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaacca tccgcaaacc gagccgcagt tggacgaaaa ccaaatcatc gccctgcgcc gcgaaaaact gaacaacatc cgccaacagc gcaacgctta tcccaacgat ttcaagcgcg acagctttgc cgccgatttg caggcgcaat acggcgaaat cggcaaagaa gaactcgacc cgcaggccgt tcccgttaaa attgccggcc gcatgatgct caaacgtcaa atgggcaaag ccagcttcgc caccattcaa gacgttaccg gtcaaatcca gctttatctg aacaacaaag gcgtgagcca agaagttttg gacgacttca accactggga cttgggcgac atcgtcggtg cggaaggcac tttgttcaaa accaaccacg gcgaactaac cgtgcgtgtg tccgacatcc gcctgctgtc caaatccctg cgtccgctgc ccgacaaaca caaaggcttg agcgatcagg aaaccaaata ccgccaacgc tacgtcgatt tgattgccaa tgaagaatcg cgcaatacct ttatcaaacg cagccaaatc atccaatctg tgcgtaattt tatggtgggc gagcattatc tcgaagtcga aaccccgatg atgcacccga ttcccggcgg cgcgacagcg aaacccttcg ttacccacca caatgccttg gacattccgc tctacctgcg catcgcgccc ganctgtatc tgaaacgctt ggtggtcggc ggnttggaac gcgtgnttga aatcaaccgc agcttccgta acgagggcat gnccgtgcnc cacaaccccn naatttncga tgattgaatt tacgaanncn tnntccgact acgaancgnn ngatgcngat ggncngaann anntcatccg caannncatc gcngcacggn aaacggcacn ggcaaannnt cannnnnngg gcaaagaant cnattngnna nnccnntcna nnnnnnganc nttcctcnnn nccaaaaatn nnnncnnnnc tnnacccnnn nnnnnnnnnn annnngnnng nnnnnnnnnn nnnnnnnnnn naaannnnng nnncnnccgn nnntngnnnn nnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SNHPQTEPQL DENQIIALRR EKLNNIRQQR NAYPNDFKRD SFAADLQAQY GEIGKEELDP QAVPVKIAGR MMLKRQMGKA SFATIQDVTG QIQLYLNNKG VSQEVLDDFN HWDLGDIVGA EGTLFKTNHG ELTVRVSDIR LLSKSLRPLP DKHKGLSDQE TKYRQRYVDL IANEESRNTF IKRSQIIQSV RNFMVGEHYL EVETPMMHPI PGGATAKPFV THHNALDIPL YLRIAPELYL KRLVVGGLER VFEINRSFRN EGMSVRHNPE FTMIEFYEAF SDYERMMQMA EDIIRNASRT VNGTANITYN GKEVDLESPF ERLTILEAIK KYNPHYTDEQ LNDAEWLAAE IVKHGESLPP SPGIGSLQLA LFEGTAEGKL WNPTFIVDYP VEVSPLARAS DTKQGLTERF ELFVVGRELA NGYSELNDPE DQAERFKSQV AQKDAGDDEA MHYDADYIRA MEFGLPPTGG CGIGIDRLVM LLTDLQTIRD VILFPQMRPE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgaa ccatccgcaa accgagccgc agttggacga aaaccaaatc atcgccctgc gccgcgaaaa actgaacaac atccgccaac agcgcaacgc ttaccccaac gatttcaagc gcgacagctt tgccgccgat ttgcaggcgc aatacggcga aatcggcaaa gaagaactcg acccgcaggc cgttcccgtt aaaattgccg gccgcatgat gctcaaacgt caaatgggca aagccagctt cgccaccatt caagacgtta ccggtcaaat ccagctttat ctgaacaaca aaggcgtgag ccaagaagtt ttggacgact tcaaccactg ggacttgggc gacatcgtcg gtgcggaagg cactttgttc aaaaccaacc acggcgaact aaccgtgcgt gtgtccgaca tccgcctgct gtccaaatcc ctgcgtccgc tgcccgacaa acacaaaggc ttgagcgatc aggaaaccaa ataccgccaa cgctacgtcg atttgattgc caatgaagaa tcgcgcaata cctttatcaa acgcagccaa atcatccaat ctgtgcgtaa ttttatggtg ggcgagcatt atctcgaagt cgaaaccccg atgatgcacc cgattcccgg cggcgcgaca gcgaaaccct tcgttaccca ccacaatgcc ttggacattc cgctctacct gcgcatcgcg cccgagctgt atctgaaacg cttggtggtc ggcggcttgg aacgcgtgtt tgaaatcaac cgcagcttcc gtaacgaggg catgtccgtg cgccacaacc ccgaatttac gatgattgaa ttttacgaag ccttctccga ctacgaacgt atgatgcaga tggcggaaga catcatccgc aacgcatcgc gcacggtaaa cggcacggca aacatcactt acaacggcaa agaagtcgat ttggaaagcc cgttcgaacg tctgaccatc ctcgaagcca tcaaaaaata caatccgcac tacaccgacg agcagttgaa cgatgcggaa tggctggcag ctgaaatcgt caaacacggc gaaagcctgc cgccgtcccc gggcatcggc agcctgcaac tcgccctgtt tgaaggtacc gccgagggca aactgtggaa tccgaccttt attgtcgatt acccggtcga agtttccccg ttggcgcgcg cttcggatac gaaacaaggt ctgaccgaac gcttcgagct tttcgttgtc gggcgcgaac tggcaaacgg ctattccgag ttgaacgacc ccgaagacca agccgaacgc ttcaaatcgc aagtggcgca aaaagatgcg ggcgacgacg aagccatgca ctacgatgcc gactacatcc gcgcgatgga attcggtttg ccgccgaccg gcggttgcgg catcggcatc gaccgcttag tgatgctgct gaccgatttg caaaccatcc gcgatgtgat tctgtttccg cagatgcgtc ccgagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for NegoA.00612.a.A15.GE43347

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	59kDa
OBSERVED MW:	59kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	56

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPQTEPQLD ENQIIALRRE KLNNIRQQRN AYPNDFKRDS FAADLQAQYG EIGKEELDPQ AVPVKIAGRM MLKRQMGKAS FATIQDVTGQ IQLYLNNKGV SQEVLDDFNH WDLGDIVGAE GTLFKTNHGE LTVRVSDIRL LSKSLRPLPD KHKGLSDQET KYRQRYVDLI ANEESRNTFI KRSXIIQSVR NFMVGEHYLE VETPMMHPIP GGATAKPFVT HHNALDIPLY LRIAPELXLK RLXVGGLERV FEINRSXRNE GMSXRHNPEX YDD

Validated NT Sequence

acgactcnct atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcaaacc gagccgcagt tggacgaaaa ccaaatcatc gccctgcgcc gcgaaaaact gaacaacatc cgccaacagc gcaacgctta tcccaacgat ttcaagcgcg acagctttgc cgccgatttg caggcgcaat acggcgaaat cggcaaagaa gaactcgacc cgcaggccgt tcccgttaaa attgccggcc gcatgatgct caaacgtcaa atgggcaaag ccagcttcgc caccattcaa gacgttaccg gtcaaatcca gctttatctg aacaacaaag gcgtgagcca agaagttttg gacgacttca accactggga cttgggcgac atcgtcggtg cggaaggcac tttgttcaaa accaaccacg gcgaactaac cgtgcgtgtg tccgacatcc gcctgctgtc caaatccctg cgtccgctgc ccgacaaaca caaaggcttg agcgatcagg aaaccaaata ccgccaacgc tacgtcgatt tgattgccaa tgaagaatcg cgcaatacct ttatcaaacg cagccnaatc atccaatctg tgcgtaattt tatggtgggc gagcattatc tcgaagtcga aaccccgatg atgcacccga ttcccggcgg cgcgacagcg aaacccttcg ttacccacca caatgccttg gacattccgc tctacctgcg catcgcgccc gagctgnatc tgaaacgctt ggnggtcggc ggcttggaac gcgtgtttga aatcaaccgc agcttncgta acgagggcat gtccgngcgc cacaaccccg aantttacga tgattgantt ttacgannct ncntccgact acnangtatg atgcnnatgg cggannnnnt catcncnacg catcnnnnac gnaancggna cggnaanntc nnttacanng naanannnna nttnnnnncc nnnnacgtnt gacntnntcn ancntnaaan nnnanncnnn nnnnnnnnac nnnnnntnan nannnnnnnn gnnngnncnn nnnnnnnnnn nnancngcnn ngnnntngnn nnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SPQTEPQLDE NQIIALRREK LNNIRQQRNA YPNDFKRDSF AADLQAQYGE IGKEELDPQA VPVKIAGRMM LKRQMGKASF ATIQDVTGQI QLYLNNKGVS QEVLDDFNHW DLGDIVGAEG TLFKTNHGEL TVRVSDIRLL SKSLRPLPDK HKGLSDQETK YRQRYVDLIA NEESRNTFIK RSQIIQSVRN FMVGEHYLEV ETPMMHPIPG GATAKPFVTH HNALDIPLYL RIAPELYLKR LVVGGLERVF EINRSFRNEG MSVRHNPEFT MIEFYEAFSD YERMMQMAED IIRNASRTVN GTANITYNGK EVDLESPFER LTILEAIKKY NPHYTDEQLN DAEWLAAEIV KHGESLPPSP GIGSLQLALF EGTAEGKLWN PTFIVDYPVE VSPLARASDT KQGLTERFEL FVVGRELANG YSELNDPEDQ AERFKSQVAQ KDAGDDEAMH YDADYIRAME FGLPPTGGCG IGIDRLVMLL TDLQTIRDVI LFPQMRPE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgcc gcaaaccgag ccgcagttgg acgaaaacca aatcatcgcc ctgcgccgcg aaaaactgaa caacatccgc caacagcgca acgcttaccc caacgatttc aagcgcgaca gctttgccgc cgatttgcag gcgcaatacg gcgaaatcgg caaagaagaa ctcgacccgc aggccgttcc cgttaaaatt gccggccgca tgatgctcaa acgtcaaatg ggcaaagcca gcttcgccac cattcaagac gttaccggtc aaatccagct ttatctgaac aacaaaggcg tgagccaaga agttttggac gacttcaacc actgggactt gggcgacatc gtcggtgcgg aaggcacttt gttcaaaacc aaccacggcg aactaaccgt gcgtgtgtcc gacatccgcc tgctgtccaa atccctgcgt ccgctgcccg acaaacacaa aggcttgagc gatcaggaaa ccaaataccg ccaacgctac gtcgatttga ttgccaatga agaatcgcgc aataccttta tcaaacgcag ccaaatcatc caatctgtgc gtaattttat ggtgggcgag cattatctcg aagtcgaaac cccgatgatg cacccgattc ccggcggcgc gacagcgaaa cccttcgtta cccaccacaa tgccttggac attccgctct acctgcgcat cgcgcccgag ctgtatctga aacgcttggt ggtcggcggc ttggaacgcg tgtttgaaat caaccgcagc ttccgtaacg agggcatgtc cgtgcgccac aaccccgaat ttacgatgat tgaattttac gaagccttct ccgactacga acgtatgatg cagatggcgg aagacatcat ccgcaacgca tcgcgcacgg taaacggcac ggcaaacatc acttacaacg gcaaagaagt cgatttggaa agcccgttcg aacgtctgac catcctcgaa gccatcaaaa aatacaatcc gcactacacc gacgagcagt tgaacgatgc ggaatggctg gcagctgaaa tcgtcaaaca cggcgaaagc ctgccgccgt ccccgggcat cggcagcctg caactcgccc tgtttgaagg taccgccgag ggcaaactgt ggaatccgac ctttattgtc gattacccgg tcgaagtttc cccgttggcg cgcgcttcgg atacgaaaca aggtctgacc gaacgcttcg agcttttcgt tgtcgggcgc gaactggcaa acggctattc cgagttgaac gaccccgaag accaagccga acgcttcaaa tcgcaagtgg cgcaaaaaga tgcgggcgac gacgaagcca tgcactacga tgccgactac atccgcgcga tggaattcgg tttgccgccg accggcggtt gcggcatcgg catcgaccgc ttagtgatgc tgctgaccga tttgcaaacc atccgcgatg tgattctgtt tccgcagatg cgtcccgagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for NegoA.00612.a.A17.GE43349

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	95
PERCENT COVERAGE:	55

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPQLDENQI IALRREKLNN IRQQRNAYPN DFKRDSFAAD LQAQYGEIGK EELDPQAVPV KIAGRMMLKR QMGKASFATI QDVTGQIQLY LNNKGVSQEV LDDFNHWDLG DIVGAEGTLF KTNHGELTVR VSDIRLLSKS LRPLPDKHKG LSDQETKYRQ RYVDLIANEE SRNTFIKRSQ IIQSVRNFMV GEHYLEVETP MMHPIPGGAT AKPFVTHHNA LDIPLYLRIA PELYLKRLVV GGLERVFEST AASVTRACPC ATTPNLR

Validated NT Sequence

gnnnnnnnnn nnnnnnnnnn acgactcnct atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactga acaacatccg ccaacagcgc aacgcttatc ccaacgattt caagcgcgac agctttgccg ccgatttgca ggcgcaatac ggcgaaatcg gcaaagaaga actcgacccg caggccgttc ccgttaaaat tgccggccgc atgatgctca aacgtcaaat gggcaaagcc agcttcgcca ccattcaaga cgttaccggt caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga cgacttcaac cactgggact tgggcgacat cgtcggtgcg gaaggcactt tgttcaaaac caaccacggc gaactaaccg tgcgtgtgtc cgacatccgc ctgctgtcca aatccctgcg tccgctgccc gacaaacaca aaggcttgag cgatcaggaa accaaatacc gccaacgcta cgtcgatttg attgccaatg aagaatcgcg caataccttt atcaaacgca gccaaatcat ccaatctgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat gcacccgatt cccggcggcg cgacagcgaa acccttcgtt acccaccaca atgccttgga cattccgctc tacctgcgca tcgcgcccga gctgtatctg aaacgcttgg tggtcggcgg cttggaacgc gtgtttgaat caaccgcagc ttccgtaacg agggcatgtc cgtgcgccac aaccccgaat ttacgatgat tgaattttac gaagccttct ccgactacga acgtatgatg cagatggcgg ananntcatc cgcaacgcnt cgcgcacggn naaacggcac ngnnancatc acttanangg nnngaagtcg atttngaaag cccnttcgaa cgtcnnacna nctcnannnt naaaaantan annccnnncn tannnnnacg ancnnnnang angcnnngnn nnnncnnaan ncnnnannnn ggcnnnnncc nnnnnngnnn nnnnnannnn ccccnnnttn nnng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SPQLDENQII ALRREKLNNI RQQRNAYPND FKRDSFAADL QAQYGEIGKE ELDPQAVPVK IAGRMMLKRQ MGKASFATIQ DVTGQIQLYL NNKGVSQEVL DDFNHWDLGD IVGAEGTLFK TNHGELTVRV SDIRLLSKSL RPLPDKHKGL SDQETKYRQR YVDLIANEES RNTFIKRSQI IQSVRNFMVG EHYLEVETPM MHPIPGGATA KPFVTHHNAL DIPLYLRIAP ELYLKRLVVG GLERVFEINR SFRNEGMSVR HNPEFTMIEF YEAFSDYERM MQMAEDIIRN ASRTVNGTAN ITYNGKEVDL ESPFERLTIL EAIKKYNPHY TDEQLNDAEW LAAEIVKHGE SLPPSPGIGS LQLALFEGTA EGKLWNPTFI VDYPVEVSPL ARASDTKQGL TERFELFVVG RELANGYSEL NDPEDQAERF KSQVAQKDAG DDEAMHYDAD YIRAMEFGLP PTGGCGIGID RLVMLLTDLQ TIRDVILFPQ MRPE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgcc gcagttggac gaaaaccaaa tcatcgccct gcgccgcgaa aaactgaaca acatccgcca acagcgcaac gcttacccca acgatttcaa gcgcgacagc tttgccgccg atttgcaggc gcaatacggc gaaatcggca aagaagaact cgacccgcag gccgttcccg ttaaaattgc cggccgcatg atgctcaaac gtcaaatggg caaagccagc ttcgccacca ttcaagacgt taccggtcaa atccagcttt atctgaacaa caaaggcgtg agccaagaag ttttggacga cttcaaccac tgggacttgg gcgacatcgt cggtgcggaa ggcactttgt tcaaaaccaa ccacggcgaa ctaaccgtgc gtgtgtccga catccgcctg ctgtccaaat ccctgcgtcc gctgcccgac aaacacaaag gcttgagcga tcaggaaacc aaataccgcc aacgctacgt cgatttgatt gccaatgaag aatcgcgcaa tacctttatc aaacgcagcc aaatcatcca atctgtgcgt aattttatgg tgggcgagca ttatctcgaa gtcgaaaccc cgatgatgca cccgattccc ggcggcgcga cagcgaaacc cttcgttacc caccacaatg ccttggacat tccgctctac ctgcgcatcg cgcccgagct gtatctgaaa cgcttggtgg tcggcggctt ggaacgcgtg tttgaaatca accgcagctt ccgtaacgag ggcatgtccg tgcgccacaa ccccgaattt acgatgattg aattttacga agccttctcc gactacgaac gtatgatgca gatggcggaa gacatcatcc gcaacgcatc gcgcacggta aacggcacgg caaacatcac ttacaacggc aaagaagtcg atttggaaag cccgttcgaa cgtctgacca tcctcgaagc catcaaaaaa tacaatccgc actacaccga cgagcagttg aacgatgcgg aatggctggc agctgaaatc gtcaaacacg gcgaaagcct gccgccgtcc ccgggcatcg gcagcctgca actcgccctg tttgaaggta ccgccgaggg caaactgtgg aatccgacct ttattgtcga ttacccggtc gaagtttccc cgttggcgcg cgcttcggat acgaaacaag gtctgaccga acgcttcgag cttttcgttg tcgggcgcga actggcaaac ggctattccg agttgaacga ccccgaagac caagccgaac gcttcaaatc gcaagtggcg caaaaagatg cgggcgacga cgaagccatg cactacgatg ccgactacat ccgcgcgatg gaattcggtt tgccgccgac cggcggttgc ggcatcggca tcgaccgctt agtgatgctg ctgaccgatt tgcaaaccat ccgcgatgtg attctgtttc cgcagatgcg tcccgagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for NegoA.00612.a.A18.GE43350

HARVESTED ON:	7/31/2019
SEQUENCED ON:	8/7/2019
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	79
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLDENQIIA LRREKLNNIR QQRNAYPNDF KRDSFAADLQ AQYGEIGKEE LDPQAVPVKI AGRMMLKRQM GKASFATIQD VTGQIQLYLN NKGVSQEVLD DFNHWDLGDI VGAEGTLFKT NHGELTVRVS DIRLLSKSLR PLPDKHKGLS DQETKYRQRY VDLIANEESR NTFIKRSQII QSVRNFMVGE HYLEVETPMM HPIPGGATAK PFVTHHNALD IPLYLRIAPE LYLNAWXSAA WKRVFEINRS FRNEGMSVRH XPEFTMIEFY EAFSDYXRMM QMXXXXPQRI AXGXXXXXSY XGXXSIXKPV XXSDPXXXKX YXXXXXXRXX XXXXXXXXXX XXXXXXXXXX XXXX

Validated NT Sequence

ggnnnnnnnn cnnntatacg actcnctata gggagancac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgtt ggacgaaaac caaatcatcg ccctgcgccg cgaaaaactg aacaacatcc gccaacagcg caacgcttat cccaacgatt tcaagcgcga cagctttgcc gccgatttgc aggcgcaata cggcgaaatc ggcaaagaag aactcgaccc gcaggccgtt cccgttaaaa ttgccggccg catgatgctc aaacgtcaaa tgggcaaagc cagcttcgcc accattcaag acgttaccgg tcaaatccag ctttatctga acaacaaagg cgtgagccaa gaagttttgg acgacttcaa ccactgggac ttgggcgaca tcgtcggtgc ggaaggcact ttgttcaaaa ccaaccacgg cgaactaacc gtgcgtgtgt ccgacatccg cctgctgtcc aaatccctgc gtccgctgcc cgacaaacac aaaggcttga gcgatcagga aaccaaatac cgccaacgct acgtcgattt gattgccaat gaagaatcgc gcaatacctt tatcaaacgc agccaaatca tccaatctgt gcgtaatttt atggtgggcg agcattatct cgaagtcgaa accccgatga tgcacccgat tcccggcggc gcgacagcga aacccttcgt tacccaccac aatgccttgg acattccgct ctacctgcgc atcgcgcccg agctgtatct gaacgcttgg nggtcggcgg cttggaaacg cgtgtttgaa atcaaccgca gcttccgtaa cgagggcatg tccgtgcgcc acnaccccga atttacgatg attgaatttt acgaagcctt ctccgactac naacgtatga tgcagatggn gnnnnntcnt ccgcaacgca tcgcnnacgg naanngcann naannatctt acnacggnna nnantcgatt ngnaagcccg tncnanngtc ngaccctcna ncnntcaaaa antacnannc nnnnntannc cnacgannnn nnnannnatg nnnnnnnnnn nnnnnnannn nnnnnnngnn nnnnncnnnn nccnnntngg nnnnnnnaan tngccc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SLDENQIIAL RREKLNNIRQ QRNAYPNDFK RDSFAADLQA QYGEIGKEEL DPQAVPVKIA GRMMLKRQMG KASFATIQDV TGQIQLYLNN KGVSQEVLDD FNHWDLGDIV GAEGTLFKTN HGELTVRVSD IRLLSKSLRP LPDKHKGLSD QETKYRQRYV DLIANEESRN TFIKRSQIIQ SVRNFMVGEH YLEVETPMMH PIPGGATAKP FVTHHNALDI PLYLRIAPEL YLKRLVVGGL ERVFEINRSF RNEGMSVRHN PEFTMIEFYE AFSDYERMMQ MAEDIIRNAS RTVNGTANIT YNGKEVDLES PFERLTILEA IKKYNPHYTD EQLNDAEWLA AEIVKHGESL PPSPGIGSLQ LALFEGTAEG KLWNPTFIVD YPVEVSPLAR ASDTKQGLTE RFELFVVGRE LANGYSELND PEDQAERFKS QVAQKDAGDD EAMHYDADYI RAMEFGLPPT GGCGIGIDRL VMLLTDLQTI RDVILFPQMR PE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgtt ggacgaaaac caaatcatcg ccctgcgccg cgaaaaactg aacaacatcc gccaacagcg caacgcttac cccaacgatt tcaagcgcga cagctttgcc gccgatttgc aggcgcaata cggcgaaatc ggcaaagaag aactcgaccc gcaggccgtt cccgttaaaa ttgccggccg catgatgctc aaacgtcaaa tgggcaaagc cagcttcgcc accattcaag acgttaccgg tcaaatccag ctttatctga acaacaaagg cgtgagccaa gaagttttgg acgacttcaa ccactgggac ttgggcgaca tcgtcggtgc ggaaggcact ttgttcaaaa ccaaccacgg cgaactaacc gtgcgtgtgt ccgacatccg cctgctgtcc aaatccctgc gtccgctgcc cgacaaacac aaaggcttga gcgatcagga aaccaaatac cgccaacgct acgtcgattt gattgccaat gaagaatcgc gcaatacctt tatcaaacgc agccaaatca tccaatctgt gcgtaatttt atggtgggcg agcattatct cgaagtcgaa accccgatga tgcacccgat tcccggcggc gcgacagcga aacccttcgt tacccaccac aatgccttgg acattccgct ctacctgcgc atcgcgcccg agctgtatct gaaacgcttg gtggtcggcg gcttggaacg cgtgtttgaa atcaaccgca gcttccgtaa cgagggcatg tccgtgcgcc acaaccccga atttacgatg attgaatttt acgaagcctt ctccgactac gaacgtatga tgcagatggc ggaagacatc atccgcaacg catcgcgcac ggtaaacggc acggcaaaca tcacttacaa cggcaaagaa gtcgatttgg aaagcccgtt cgaacgtctg accatcctcg aagccatcaa aaaatacaat ccgcactaca ccgacgagca gttgaacgat gcggaatggc tggcagctga aatcgtcaaa cacggcgaaa gcctgccgcc gtccccgggc atcggcagcc tgcaactcgc cctgtttgaa ggtaccgccg agggcaaact gtggaatccg acctttattg tcgattaccc ggtcgaagtt tccccgttgg cgcgcgcttc ggatacgaaa caaggtctga ccgaacgctt cgagcttttc gttgtcgggc gcgaactggc aaacggctat tccgagttga acgaccccga agaccaagcc gaacgcttca aatcgcaagt ggcgcaaaaa gatgcgggcg acgacgaagc catgcactac gatgccgact acatccgcgc gatggaattc ggtttgccgc cgaccggcgg ttgcggcatc ggcatcgacc gcttagtgat gctgctgacc gatttgcaaa ccatccgcga tgtgattctg tttccgcaga tgcgtcccga gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for NegoA.00612.a.B1.GE39969

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	70
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS EQNHPQTEPQ LDENQIIALR REKLNNIRQQ RNAYPNDFKR DSFAADLQAQ YGEIGKEELD PQAVPVKIAG RMMLKRQMGK ASFATIQDVT GQIQLYLNNK GVSQEVLDDF NHWDLGDIVG AEGTLFKTNH GELTVRVSDI RLLSKSLRPL PDKHKGLSDQ ETKYRQRYVD LIANEESRNT FIKRSQIIQS VRNFMVGEHY LEVETPMMHP IPGGATAKPF VTHHNALDIP LYLRIAXXLY LKRLVXXGXX RVXXINRXXR NEXXSVXXXX XXXXIXXXXX XXXXXXXXXX XXXIIRNAXX XXXXXANITY XXKXXDLEXX XXXXXXXXAI KKYXPXXXXE XXXXXEXXKX XIVXXGESLP PXXGIGSLQX XXXXXCAEGX LWNPTFIVXX XVEVSPXARX XXXXQGXXXX XXLXXVGRXL AXXXXXLXXX XXXXXXXXXX XXXXXXXXXX AXXXXXXYIR AMXFXXXPTG XXGIGXXXXV XXXXDLQTIR D

Validated NT Sequence

catcgcggat ggtttgcaaa tcggnnnnnn nnatcactan nnnntcgntg ccgatgccnn nnncgccggt cggcngnnnn ncgaattnca tcgcgcggat gtagnnnnnn tcgtnnnnna tggcnnnnnn nnnnnncgnn nnnnnntgnn nnnntnnnnn nttnnngnnn nnnnnttnnt cnnnnnnnnn nntcaactnn nnnnngcnnn nngccaannc gcgcccgacn nnnnaaagct nnnnnngttn nnnnnnncct tgtnnnnnnt ccnnnnngcg cgcnnnnggg gaaacttcga ccgnnnnnnc cacaataaag gtcggattcc acagtnnncc ctcggcgcaa cntncnnnnn nnnnnanttg naggctgccg atgccnnnnn ncggcggcag gctttcgccg nnnttgacga tttnnntttt nnnnnnttcn nnatnnnnnn nntgctcgnn nnnntnnngc ggntngtatt ttttgatggc tnnnnnnnnn nnnnnnnnnn nnaannnnnt ttccaaatcg annnctttgn cnttgtaagt gatgtttgcc nnnnnnnnnn cnnnnnnnnn ngcgttgcgg atgatgtnnn nnnnnnnnnn nnncanannn tnnnnnnnnn ngnnnnnnnn ntnnnnnnca atcnnnnnnn nnnnnnnnnn nnnnnncacg gacanncnct cgttacgnnn nctgcggttg attnnnnaca cgcgtnnnaa gccnnnnncc accaagcgtt tcagatacag nnnnngcgcg atgcgcaggt agagcggaat gtccaaggca ttgtggtggg taacgaaggg tttcgctgtc gcgccgccgg gaatcgggtg catcatcggg gtttcgactt cgagataatg ctcgcccacc ataaaattac gcacagattg gatgatttgg ctgcgtttga taaaggtatt gcgcgattct tcattggcaa tcaaatcgac gtagcgttgg cggtatttgg tttcctgatc gctcaagcct ttgtgtttgt cgggcagcgg acgcagggat ttggacagca ggcggatgtc ggacacacgc acggttagtt cgccgtggtt ggttttgaac aaagtgcctt ccgcaccgac gatgtcgccc aagtcccagt ggttgaagtc gtccaaaact tcttggctca cgcctttgtt gttcagataa agctggattt gaccggtaac gtcttgaatg gtggcgaagc tggctttgcc catttgacgt ttgagcatca tgcggccggc aattttaacg ggaacggcct gcgggtcgag ttcttctttg ccgatttcgc cgtattgcgc ctgcaaatcg gcggcaaagc tgtcgcgctt gaaatcgttg ggataagcgt tgcgctgttg gcggatgttg ttcagttttt cgcggcgcag ggcgatgatt tggttttcgt ccaactgcgg ctcggtttgc ggatggtttt gttcgctcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMS EQNHPQTEPQ LDENQIIALR REKLNNIRQQ RNAYPNDFKR DSFAADLQAQ YGEIGKEELD PQAVPVKIAG RMMLKRQMGK ASFATIQDVT GQIQLYLNNK GVSQEVLDDF NHWDLGDIVG AEGTLFKTNH GELTVRVSDI RLLSKSLRPL PDKHKGLSDQ ETKYRQRYVD LIANEESRNT FIKRSQIIQS VRNFMVGEHY LEVETPMMHP IPGGATAKPF VTHHNALDIP LYLRIAPELY LKRLVVGGLE RVFEINRSFR NEGMSVRHNP EFTMIEFYEA FSDYERMMQM AEDIIRNASR TVNGTANITY NGKEVDLESP FERLTILEAI KKYNPHYTDE QLNDAEWLKK EIVKHGESLP PSPGIGSLQL ALFEGCAEGK LWNPTFIVDY PVEVSPLARA SDTKQGLTER FELFVVGREL ANGYSELNDP EDQAERFKSQ VAQKDAGDDE AMHYDADYIR AMEFGLPPTG GCGIGIDRLV MLLTDLQTIR DVILFPQMRP E

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg aacaaaacca tccgcaaacc gagccgcagt tggacgaaaa ccaaatcatc gccctgcgcc gcgaaaaact gaacaacatc cgccaacagc gcaacgctta ccccaacgat ttcaagcgcg acagctttgc cgccgatttg caggcgcaat acggcgaaat cggcaaagaa gaactcgacc cgcaggccgt tcccgttaaa attgccggcc gcatgatgct caaacgtcaa atgggcaaag ccagcttcgc caccattcaa gacgttaccg gtcaaatcca gctttatctg aacaacaaag gcgtgagcca agaagttttg gacgacttca accactggga cttgggcgac atcgtcggtg cggaaggcac tttgttcaaa accaaccacg gcgaactaac cgtgcgtgtg tccgacatcc gcctgctgtc caaatccctg cgtccgctgc ccgacaaaca caaaggcttg agcgatcagg aaaccaaata ccgccaacgc tacgtcgatt tgattgccaa tgaagaatcg cgcaatacct ttatcaaacg cagccaaatc atccaatctg tgcgtaattt tatggtgggc gagcattatc tcgaagtcga aaccccgatg atgcacccga ttcccggcgg cgcgacagcg aaacccttcg ttacccacca caatgccttg gacattccgc tctacctgcg catcgcgccc gagctgtatc tgaaacgctt ggtggtcggc ggcttggaac gcgtgtttga aatcaaccgc agcttccgta acgagggcat gtccgtgcgc cacaaccccg aatttacgat gattgaattt tacgaagcct tctccgacta cgaacgtatg atgcagatgg cggaagacat catccgcaac gcatcgcgca cggtaaacgg cacggcaaac atcacttaca acggcaaaga agtcgatttg gaaagcccgt tcgaacgtct gaccatcctc gaagccatca aaaaatacaa tccgcactac accgacgagc agttgaacga tgcggaatgg ctgaaaaaag aaatcgtcaa acacggcgaa agcctgccgc cgtccccggg catcggcagc ctgcaactcg ccctgtttga aggttgcgcc gagggcaaac tgtggaatcc gacctttatt gtcgattacc cggtcgaagt ttccccgttg gcgcgcgctt cggatacgaa acaaggtctg accgaacgct tcgagctttt cgttgtcggg cgcgaactgg caaacggcta ttccgagttg aacgaccccg aagaccaagc cgaacgcttc aaatcgcaag tggcgcaaaa agatgcgggc gacgacgaag ccatgcacta cgatgccgac tacatccgcg cgatggaatt cggtttgccg ccgaccggcg gttgcggcat cggcatcgac cgcttagtga tgctgctgac cgatttgcaa accatccgcg atgtgattct gtttccgcag atgcgtcccg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for NegoA.00612.a.B1.PW37916

PURIFICATION DATe:	6/7/2016
CONCENTRATION:	31mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	19
VIAL VOLUME:	200µl
PERCENT IDENTITY:	70
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMS EQNHPQTEPQ LDENQIIALR REKLNNIRQQ RNAYPNDFKR DSFAADLQAQ YGEIGKEELD PQAVPVKIAG RMMLKRQMGK ASFATIQDVT GQIQLYLNNK GVSQEVLDDF NHWDLGDIVG AEGTLFKTNH GELTVRVSDI RLLSKSLRPL PDKHKGLSDQ ETKYRQRYVD LIANEESRNT FIKRSQIIQS VRNFMVGEHY LEVETPMMHP IPGGATAKPF VTHHNALDIP LYLRIAPELY LKRLVVGGLE RVFEINRSFR NEGMSVRHNP EFTMIEFYEA FSDYERMMQM AEDIIRNASR TVNGTANITY NGKEVDLESP FERLTILEAI KKYNPHYTDE QLNDAEWLKK EIVKHGESLP PSPGIGSLQL ALFEGCAEGK LWNPTFIVDY PVEVSPLARA SDTKQGLTER FELFVVGREL ANGYSELNDP EDQAERFKSQ VAQKDAGDDE AMHYDADYIR AMEFGLPPTG GCGIGIDRLV MLLTDLQTIR DVILFPQMRP E

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG AACAAAACCA TCCGCAAACC GAGCCGCAGT TGGACGAAAA CCAAATCATC GCCCTGCGCC GCGAAAAACT GAACAACATC CGCCAACAGC GCAACGCTTA CCCCAACGAT TTCAAGCGCG ACAGCTTTGC CGCCGATTTG CAGGCGCAAT ACGGCGAAAT CGGCAAAGAA GAACTCGACC CGCAGGCCGT TCCCGTTAAA ATTGCCGGCC GCATGATGCT CAAACGTCAA ATGGGCAAAG CCAGCTTCGC CACCATTCAA GACGTTACCG GTCAAATCCA GCTTTATCTG AACAACAAAG GCGTGAGCCA AGAAGTTTTG GACGACTTCA ACCACTGGGA CTTGGGCGAC ATCGTCGGTG CGGAAGGCAC TTTGTTCAAA ACCAACCACG GCGAACTAAC CGTGCGTGTG TCCGACATCC GCCTGCTGTC CAAATCCCTG CGTCCGCTGC CCGACAAACA CAAAGGCTTG AGCGATCAGG AAACCAAATA CCGCCAACGC TACGTCGATT TGATTGCCAA TGAAGAATCG CGCAATACCT TTATCAAACG CAGCCAAATC ATCCAATCTG TGCGTAATTT TATGGTGGGC GAGCATTATC TCGAAGTCGA AACCCCGATG ATGCACCCGA TTCCCGGCGG CGCGACAGCG AAACCCTTCG TTACCCACCA CAATGCCTTG GACATTCCGC TCTACCTGCG CATCGCGCCC GAGCTGTATC TGAAACGCTT GGTGGTCGGC GGCTTGGAAC GCGTGTTTGA AATCAACCGC AGCTTCCGTA ACGAGGGCAT GTCCGTGCGC CACAACCCCG AATTTACGAT GATTGAATTT TACGAAGCCT TCTCCGACTA CGAACGTATG ATGCAGATGG CGGAAGACAT CATCCGCAAC GCATCGCGCA CGGTAAACGG CACGGCAAAC ATCACTTACA ACGGCAAAGA AGTCGATTTG GAAAGCCCGT TCGAACGTCT GACCATCCTC GAAGCCATCA AAAAATACAA TCCGCACTAC ACCGACGAGC AGTTGAACGA TGCGGAATGG CTGAAAAAAG AAATCGTCAA ACACGGCGAA AGCCTGCCGC CGTCCCCGGG CATCGGCAGC CTGCAACTCG CCCTGTTTGA AGGTTGCGCC GAGGGCAAAC TGTGGAATCC GACCTTTATT GTCGATTACC CGGTCGAAGT TTCCCCGTTG GCGCGCGCTT CGGATACGAA ACAAGGTCTG ACCGAACGCT TCGAGCTTTT CGTTGTCGGG CGCGAACTGG CAAACGGCTA TTCCGAGTTG AACGACCCCG AAGACCAAGC CGAACGCTTC AAATCGCAAG TGGCGCAAAA AGATGCGGGC GACGACGAAG CCATGCACTA CGATGCCGAC TACATCCGCG CGATGGAATT CGGTTTGCCG CCGACCGGCG GTTGCGGCAT CGGCATCGAC CGCTTAGTGA TGCTGCTGAC CGATTTGCAA ACCATCCGCG ATGTGATTCT GTTTCCGCAG ATGCGTCCCG AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT

Details for NegoA.00612.a.A11.PW38713

PURIFICATION DATe:	10/8/2019
CONCENTRATION:	45mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	56

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGAACAA AACCATCCGC AAACCGAGCC GCAGTTGGAC GAAAACCAAA TCATCGCCCT GCGCCGCGAA AAACTGAACA ACATCCGCCA ACAGCGCAAC GCTTACCCCA ACGATTTCAA GCGCGACAGC TTTGCCGCCG ATTTGCAGGC GCAATACGGC GAAATCGGCA AAGAAGAACT CGACCCGCAG GCCGTTCCCG TTAAAATTGC CGGCCGCATG ATGCTCAAAC GTCAAATGGG CAAAGCCAGC TTCGCCACCA TTCAAGACGT TACCGGTCAA ATCCAGCTTT ATCTGAACAA CAAAGGCGTG AGCCAAGAAG TTTTGGACGA CTTCAACCAC TGGGACTTGG GCGACATCGT CGGTGCGGAA GGCACTTTGT TCAAAACCAA CCACGGCGAA CTAACCGTGC GTGTGTCCGA CATCCGCCTG CTGTCCAAAT CCCTGCGTCC GCTGCCCGAC AAACACAAAG GCTTGAGCGA TCAGGAAACC AAATACCGCC AACGCTACGT CGATTTGATT GCCAATGAAG AATCGCGCAA TACCTTTATC AAACGCAGCC AAATCATCCA ATCTGTGCGT AATTTTATGG TGGGCGAGCA TTATCTCGAA GTCGAAACCC CGATGATGCA CCCGATTCCC GGCGGCGCGA CAGCGAAACC CTTCGTTACC CACCACAATG CCTTGGACAT TCCGCTCTAC CTGCGCATCG CGCCCGAGCT GTATCTGAAA CGCTTGGTGG TCGGCGGCTT GGAACGCGTG TTTGAAATCA ACCGCAGCTT CCGTAACGAG GGCATGTCCG TGCGCCACAA CCCCGAATTT ACGATGATTG AATTTTACGA AGCCTTCTCC GACTACGAAC GTATGATGCA GATGGCGGAA GACATCATCC GCAACGCATC GCGCACGGTA AACGGCACGG CAAACATCAC TTACAACGGC AAAGAAGTCG ATTTGGAAAG CCCGTTCGAA CGTCTGACCA TCCTCGAAGC CATCAAAAAA TACAATCCGC ACTACACCGA CGAGCAGTTG AACGATGCGG AATGGCTGAA AAAAGAAATC GTCAAACACG GCGAAAGCCT GCCGCCGTCC CCGGGCATCG GCAGCCTGCA ACTCGCCCTG TTTGAAGGTA CCGCCGAGGG CAAACTGTGG AATCCGACCT TTATTGTCGA TTACCCGGTC GAAGTTTCCC CGTTGGCGCG CGCTTCGGAT ACGAAACAAG GTCTGACCGA ACGCTTCGAG CTTTTCGTTG TCGGGCGCGA ACTGGCAAAC GGCTATTCCG AGTTGAACGA CCCCGAAGAC CAAGCCGAAC GCTTCAAATC GCAAGTGGCG CAAAAAGATG CGGGCGACGA CGAAGCCATG CACTACGATG CCGACTACAT CCGCGCGATG GAATTCGGTT TGCCGCCGAC CGGCGGTTGC GGCATCGGCA TCGACCGCTT AGTGATGCTG CTGACCGATT TGCAAACCAT CCGCGATGTG ATTCTGTTTC CGCAGATGCG TCCCGAGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA

Details for NegoA.00612.a.A110.PW38714

PURIFICATION DATe:	10/8/2019
CONCENTRATION:	38.7mg/ml
OBSERVED MW:	58kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	19
VIAL VOLUME:	25µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCA AATCATCGCC CTGCGCCGCG AAAAACTGAA CAACATCCGC CAACAGCGCA ACGCTTACCC CAACGATTTC AAGCGCGACA GCTTTGCCGC CGATTTGCAG GCGCAATACG GCGAAATCGG CAAAGAAGAA CTCGACCCGC AGGCCGTTCC CGTTAAAATT GCCGGCCGCA TGATGCTCAA ACGTCAAATG GGCAAAGCCA GCTTCGCCAC CATTCAAGAC GTTACCGGTC AAATCCAGCT TTATCTGAAC AACAAAGGCG TGAGCCAAGA AGTTTTGGAC GACTTCAACC ACTGGGACTT GGGCGACATC GTCGGTGCGG AAGGCACTTT GTTCAAAACC AACCACGGCG AACTAACCGT GCGTGTGTCC GACATCCGCC TGCTGTCCAA ATCCCTGCGT CCGCTGCCCG ACAAACACAA AGGCTTGAGC GATCAGGAAA CCAAATACCG CCAACGCTAC GTCGATTTGA TTGCCAATGA AGAATCGCGC AATACCTTTA TCAAACGCAG CCAAATCATC CAATCTGTGC GTAATTTTAT GGTGGGCGAG CATTATCTCG AAGTCGAAAC CCCGATGATG CACCCGATTC CCGGCGGCGC GACAGCGAAA CCCTTCGTTA CCCACCACAA TGCCTTGGAC ATTCCGCTCT ACCTGCGCAT CGCGCCCGAG CTGTATCTGA AACGCTTGGT GGTCGGCGGC TTGGAACGCG TGTTTGAAAT CAACCGCAGC TTCCGTAACG AGGGCATGTC CGTGCGCCAC AACCCCGAAT TTACGATGAT TGAATTTTAC GAAGCCTTCT CCGACTACGA ACGTATGATG CAGATGGCGG AAGACATCAT CCGCAACGCA TCGCGCACGG TAAACGGCAC GGCAAACATC ACTTACAACG GCAAAGAAGT CGATTTGGAA AGCCCGTTCG AACGTCTGAC CATCCTCGAA GCCATCAAAA AATACAATCC GCACTACACC GACGAGCAGT TGAACGATGC GGAATGGCTG GCAGCTGAAA TCGTCAAACA CGGCGAAAGC CTGCCGCCGT CCCCGGGCAT CGGCAGCCTG CAACTCGCCC TGTTTGAAGG TACCGCCGAG GGCAAACTGT GGAATCCGAC CTTTATTGTC GATTACCCGG TCGAAGTTTC CCCGTTGGCG CGCGCTTCGG ATACGAAACA AGGTCTGACC GAACGCTTCG AGCTTTTCGT TGTCGGGCGC GAACTGGCAA ACGGCTATTC CGAGTTGAAC GACCCCGAAG ACCAAGCCGA ACGCTTCAAA TCGCAAGTGG CGCAAAAAGA TGCGGGCGAC GACGAAGCCA TGCACTACGA TGCCGACTAC ATCCGCGCGA TGGAATTCGG TTTGCCGCCG ACCGGCGGTT GCGGCATCGG CATCGACCGC TTAGTGATGC TGCTGACCGA TTTGCAAACC ATCCGCGATG TGATTCTGTT TCCGCAGATG CGTCCCGAGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA

Details for NegoA.00612.a.A12.PW38715

PURIFICATION DATe:	10/9/2019
CONCENTRATION:	45.5mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSEQNHPQT EPQLDENQII ALRREKLNNI RQQRNAYPND FKRDSFAADL QAQYGEIGKE ELDPQAVPVK IAGRMMLKRQ MGKASFATIQ DVTGQIQLYL NNKGVSQEVL DDFNHWDLGD IVGAEGTLFK TNHGELTVRV SDIRLLSKSL RPLPDKHKGL SDQETKYRQR YVDLIANEES RNTFIKRSQI IQSVRNFMVG EHYLEVETPM MHPIPGGATA KPFVTHHNAL DIPLYLRIAP ELYLKRLVVG GLERVFEINR SFRNEGMSVR HNPEFTMIEF YEAFSDYERM MQMAEDIIRN ASRTVNGTAN ITYNGKEVDL ESPFERLTIL EAIKKYNPHY TDEQLNDAEW LAAEIVKHGE SLPPSPGIGS LQLALFEGTA EGKLWNPTFI VDYPVEVSPL ARASDTKQGL TERFELFVVG RELANGYSEL NDPEDQAERF KSQVAQKDAG DDEAMHYDAD YIRAMEFGLP PTGGCGIGID RLVMLLTDLQ TIRDVILFPQ MRPE

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGAACAA AACCATCCGC AAACCGAGCC GCAGTTGGAC GAAAACCAAA TCATCGCCCT GCGCCGCGAA AAACTGAACA ACATCCGCCA ACAGCGCAAC GCTTACCCCA ACGATTTCAA GCGCGACAGC TTTGCCGCCG ATTTGCAGGC GCAATACGGC GAAATCGGCA AAGAAGAACT CGACCCGCAG GCCGTTCCCG TTAAAATTGC CGGCCGCATG ATGCTCAAAC GTCAAATGGG CAAAGCCAGC TTCGCCACCA TTCAAGACGT TACCGGTCAA ATCCAGCTTT ATCTGAACAA CAAAGGCGTG AGCCAAGAAG TTTTGGACGA CTTCAACCAC TGGGACTTGG GCGACATCGT CGGTGCGGAA GGCACTTTGT TCAAAACCAA CCACGGCGAA CTAACCGTGC GTGTGTCCGA CATCCGCCTG CTGTCCAAAT CCCTGCGTCC GCTGCCCGAC AAACACAAAG GCTTGAGCGA TCAGGAAACC AAATACCGCC AACGCTACGT CGATTTGATT GCCAATGAAG AATCGCGCAA TACCTTTATC AAACGCAGCC AAATCATCCA ATCTGTGCGT AATTTTATGG TGGGCGAGCA TTATCTCGAA GTCGAAACCC CGATGATGCA CCCGATTCCC GGCGGCGCGA CAGCGAAACC CTTCGTTACC CACCACAATG CCTTGGACAT TCCGCTCTAC CTGCGCATCG CGCCCGAGCT GTATCTGAAA CGCTTGGTGG TCGGCGGCTT GGAACGCGTG TTTGAAATCA ACCGCAGCTT CCGTAACGAG GGCATGTCCG TGCGCCACAA CCCCGAATTT ACGATGATTG AATTTTACGA AGCCTTCTCC GACTACGAAC GTATGATGCA GATGGCGGAA GACATCATCC GCAACGCATC GCGCACGGTA AACGGCACGG CAAACATCAC TTACAACGGC AAAGAAGTCG ATTTGGAAAG CCCGTTCGAA CGTCTGACCA TCCTCGAAGC CATCAAAAAA TACAATCCGC ACTACACCGA CGAGCAGTTG AACGATGCGG AATGGCTGGC AGCTGAAATC GTCAAACACG GCGAAAGCCT GCCGCCGTCC CCGGGCATCG GCAGCCTGCA ACTCGCCCTG TTTGAAGGTA CCGCCGAGGG CAAACTGTGG AATCCGACCT TTATTGTCGA TTACCCGGTC GAAGTTTCCC CGTTGGCGCG CGCTTCGGAT ACGAAACAAG GTCTGACCGA ACGCTTCGAG CTTTTCGTTG TCGGGCGCGA ACTGGCAAAC GGCTATTCCG AGTTGAACGA CCCCGAAGAC CAAGCCGAAC GCTTCAAATC GCAAGTGGCG CAAAAAGATG CGGGCGACGA CGAAGCCATG CACTACGATG CCGACTACAT CCGCGCGATG GAATTCGGTT TGCCGCCGAC CGGCGGTTGC GGCATCGGCA TCGACCGCTT AGTGATGCTG CTGACCGATT TGCAAACCAT CCGCGATGTG ATTCTGTTTC CGCAGATGCG TCCCGAGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA

Details for NegoA.00612.a.A14.PW38716

PURIFICATION DATe:	10/9/2019
CONCENTRATION:	31.7mg/ml
OBSERVED MW:	59kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	56

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAA CCATCCGCAA ACCGAGCCGC AGTTGGACGA AAACCAAATC ATCGCCCTGC GCCGCGAAAA ACTGAACAAC ATCCGCCAAC AGCGCAACGC TTACCCCAAC GATTTCAAGC GCGACAGCTT TGCCGCCGAT TTGCAGGCGC AATACGGCGA AATCGGCAAA GAAGAACTCG ACCCGCAGGC CGTTCCCGTT AAAATTGCCG GCCGCATGAT GCTCAAACGT CAAATGGGCA AAGCCAGCTT CGCCACCATT CAAGACGTTA CCGGTCAAAT CCAGCTTTAT CTGAACAACA AAGGCGTGAG CCAAGAAGTT TTGGACGACT TCAACCACTG GGACTTGGGC GACATCGTCG GTGCGGAAGG CACTTTGTTC AAAACCAACC ACGGCGAACT AACCGTGCGT GTGTCCGACA TCCGCCTGCT GTCCAAATCC CTGCGTCCGC TGCCCGACAA ACACAAAGGC TTGAGCGATC AGGAAACCAA ATACCGCCAA CGCTACGTCG ATTTGATTGC CAATGAAGAA TCGCGCAATA CCTTTATCAA ACGCAGCCAA ATCATCCAAT CTGTGCGTAA TTTTATGGTG GGCGAGCATT ATCTCGAAGT CGAAACCCCG ATGATGCACC CGATTCCCGG CGGCGCGACA GCGAAACCCT TCGTTACCCA CCACAATGCC TTGGACATTC CGCTCTACCT GCGCATCGCG CCCGAGCTGT ATCTGAAACG CTTGGTGGTC GGCGGCTTGG AACGCGTGTT TGAAATCAAC CGCAGCTTCC GTAACGAGGG CATGTCCGTG CGCCACAACC CCGAATTTAC GATGATTGAA TTTTACGAAG CCTTCTCCGA CTACGAACGT ATGATGCAGA TGGCGGAAGA CATCATCCGC AACGCATCGC GCACGGTAAA CGGCACGGCA AACATCACTT ACAACGGCAA AGAAGTCGAT TTGGAAAGCC CGTTCGAACG TCTGACCATC CTCGAAGCCA TCAAAAAATA CAATCCGCAC TACACCGACG AGCAGTTGAA CGATGCGGAA TGGCTGGCAG CTGAAATCGT CAAACACGGC GAAAGCCTGC CGCCGTCCCC GGGCATCGGC AGCCTGCAAC TCGCCCTGTT TGAAGGTACC GCCGAGGGCA AACTGTGGAA TCCGACCTTT ATTGTCGATT ACCCGGTCGA AGTTTCCCCG TTGGCGCGCG CTTCGGATAC GAAACAAGGT CTGACCGAAC GCTTCGAGCT TTTCGTTGTC GGGCGCGAAC TGGCAAACGG CTATTCCGAG TTGAACGACC CCGAAGACCA AGCCGAACGC TTCAAATCGC AAGTGGCGCA AAAAGATGCG GGCGACGACG AAGCCATGCA CTACGATGCC GACTACATCC GCGCGATGGA ATTCGGTTTG CCGCCGACCG GCGGTTGCGG CATCGGCATC GACCGCTTAG TGATGCTGCT GACCGATTTG CAAACCATCC GCGATGTGAT TCTGTTTCCG CAGATGCGTC CCGAGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA

Details for NegoA.00612.a.A15.PW38717

PURIFICATION DATe:	10/10/2019
CONCENTRATION:	39.8mg/ml
OBSERVED MW:	59kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	56

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCC GCAAACCGAG CCGCAGTTGG ACGAAAACCA AATCATCGCC CTGCGCCGCG AAAAACTGAA CAACATCCGC CAACAGCGCA ACGCTTACCC CAACGATTTC AAGCGCGACA GCTTTGCCGC CGATTTGCAG GCGCAATACG GCGAAATCGG CAAAGAAGAA CTCGACCCGC AGGCCGTTCC CGTTAAAATT GCCGGCCGCA TGATGCTCAA ACGTCAAATG GGCAAAGCCA GCTTCGCCAC CATTCAAGAC GTTACCGGTC AAATCCAGCT TTATCTGAAC AACAAAGGCG TGAGCCAAGA AGTTTTGGAC GACTTCAACC ACTGGGACTT GGGCGACATC GTCGGTGCGG AAGGCACTTT GTTCAAAACC AACCACGGCG AACTAACCGT GCGTGTGTCC GACATCCGCC TGCTGTCCAA ATCCCTGCGT CCGCTGCCCG ACAAACACAA AGGCTTGAGC GATCAGGAAA CCAAATACCG CCAACGCTAC GTCGATTTGA TTGCCAATGA AGAATCGCGC AATACCTTTA TCAAACGCAG CCAAATCATC CAATCTGTGC GTAATTTTAT GGTGGGCGAG CATTATCTCG AAGTCGAAAC CCCGATGATG CACCCGATTC CCGGCGGCGC GACAGCGAAA CCCTTCGTTA CCCACCACAA TGCCTTGGAC ATTCCGCTCT ACCTGCGCAT CGCGCCCGAG CTGTATCTGA AACGCTTGGT GGTCGGCGGC TTGGAACGCG TGTTTGAAAT CAACCGCAGC TTCCGTAACG AGGGCATGTC CGTGCGCCAC AACCCCGAAT TTACGATGAT TGAATTTTAC GAAGCCTTCT CCGACTACGA ACGTATGATG CAGATGGCGG AAGACATCAT CCGCAACGCA TCGCGCACGG TAAACGGCAC GGCAAACATC ACTTACAACG GCAAAGAAGT CGATTTGGAA AGCCCGTTCG AACGTCTGAC CATCCTCGAA GCCATCAAAA AATACAATCC GCACTACACC GACGAGCAGT TGAACGATGC GGAATGGCTG GCAGCTGAAA TCGTCAAACA CGGCGAAAGC CTGCCGCCGT CCCCGGGCAT CGGCAGCCTG CAACTCGCCC TGTTTGAAGG TACCGCCGAG GGCAAACTGT GGAATCCGAC CTTTATTGTC GATTACCCGG TCGAAGTTTC CCCGTTGGCG CGCGCTTCGG ATACGAAACA AGGTCTGACC GAACGCTTCG AGCTTTTCGT TGTCGGGCGC GAACTGGCAA ACGGCTATTC CGAGTTGAAC GACCCCGAAG ACCAAGCCGA ACGCTTCAAA TCGCAAGTGG CGCAAAAAGA TGCGGGCGAC GACGAAGCCA TGCACTACGA TGCCGACTAC ATCCGCGCGA TGGAATTCGG TTTGCCGCCG ACCGGCGGTT GCGGCATCGG CATCGACCGC TTAGTGATGC TGCTGACCGA TTTGCAAACC ATCCGCGATG TGATTCTGTT TCCGCAGATG CGTCCCGAGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA

Details for NegoA.00612.a.A18.PW38718

PURIFICATION DATe:	10/10/2019
CONCENTRATION:	46.6mg/ml
OBSERVED MW:	58kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	6
VIAL VOLUME:	23µl
PERCENT IDENTITY:	79
PERCENT COVERAGE:	73

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGTT GGACGAAAAC CAAATCATCG CCCTGCGCCG CGAAAAACTG AACAACATCC GCCAACAGCG CAACGCTTAC CCCAACGATT TCAAGCGCGA CAGCTTTGCC GCCGATTTGC AGGCGCAATA CGGCGAAATC GGCAAAGAAG AACTCGACCC GCAGGCCGTT CCCGTTAAAA TTGCCGGCCG CATGATGCTC AAACGTCAAA TGGGCAAAGC CAGCTTCGCC ACCATTCAAG ACGTTACCGG TCAAATCCAG CTTTATCTGA ACAACAAAGG CGTGAGCCAA GAAGTTTTGG ACGACTTCAA CCACTGGGAC TTGGGCGACA TCGTCGGTGC GGAAGGCACT TTGTTCAAAA CCAACCACGG CGAACTAACC GTGCGTGTGT CCGACATCCG CCTGCTGTCC AAATCCCTGC GTCCGCTGCC CGACAAACAC AAAGGCTTGA GCGATCAGGA AACCAAATAC CGCCAACGCT ACGTCGATTT GATTGCCAAT GAAGAATCGC GCAATACCTT TATCAAACGC AGCCAAATCA TCCAATCTGT GCGTAATTTT ATGGTGGGCG AGCATTATCT CGAAGTCGAA ACCCCGATGA TGCACCCGAT TCCCGGCGGC GCGACAGCGA AACCCTTCGT TACCCACCAC AATGCCTTGG ACATTCCGCT CTACCTGCGC ATCGCGCCCG AGCTGTATCT GAAACGCTTG GTGGTCGGCG GCTTGGAACG CGTGTTTGAA ATCAACCGCA GCTTCCGTAA CGAGGGCATG TCCGTGCGCC ACAACCCCGA ATTTACGATG ATTGAATTTT ACGAAGCCTT CTCCGACTAC GAACGTATGA TGCAGATGGC GGAAGACATC ATCCGCAACG CATCGCGCAC GGTAAACGGC ACGGCAAACA TCACTTACAA CGGCAAAGAA GTCGATTTGG AAAGCCCGTT CGAACGTCTG ACCATCCTCG AAGCCATCAA AAAATACAAT CCGCACTACA CCGACGAGCA GTTGAACGAT GCGGAATGGC TGGCAGCTGA AATCGTCAAA CACGGCGAAA GCCTGCCGCC GTCCCCGGGC ATCGGCAGCC TGCAACTCGC CCTGTTTGAA GGTACCGCCG AGGGCAAACT GTGGAATCCG ACCTTTATTG TCGATTACCC GGTCGAAGTT TCCCCGTTGG CGCGCGCTTC GGATACGAAA CAAGGTCTGA CCGAACGCTT CGAGCTTTTC GTTGTCGGGC GCGAACTGGC AAACGGCTAT TCCGAGTTGA ACGACCCCGA AGACCAAGCC GAACGCTTCA AATCGCAAGT GGCGCAAAAA GATGCGGGCG ACGACGAAGC CATGCACTAC GATGCCGACT ACATCCGCGC GATGGAATTC GGTTTGCCGC CGACCGGCGG TTGCGGCATC GGCATCGACC GCTTAGTGAT GCTGCTGACC GATTTGCAAA CCATCCGCGA TGTGATTCTG TTTCCGCAGA TGCGTCCCGA GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA

Details for NegoA.00612.a.A17.PW38753

PURIFICATION DATe:	11/10/2019
CONCENTRATION:	36.7mg/ml
OBSERVED MW:	58kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	55

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCC GCAGTTGGAC GAAAACCAAA TCATCGCCCT GCGCCGCGAA AAACTGAACA ACATCCGCCA ACAGCGCAAC GCTTACCCCA ACGATTTCAA GCGCGACAGC TTTGCCGCCG ATTTGCAGGC GCAATACGGC GAAATCGGCA AAGAAGAACT CGACCCGCAG GCCGTTCCCG TTAAAATTGC CGGCCGCATG ATGCTCAAAC GTCAAATGGG CAAAGCCAGC TTCGCCACCA TTCAAGACGT TACCGGTCAA ATCCAGCTTT ATCTGAACAA CAAAGGCGTG AGCCAAGAAG TTTTGGACGA CTTCAACCAC TGGGACTTGG GCGACATCGT CGGTGCGGAA GGCACTTTGT TCAAAACCAA CCACGGCGAA CTAACCGTGC GTGTGTCCGA CATCCGCCTG CTGTCCAAAT CCCTGCGTCC GCTGCCCGAC AAACACAAAG GCTTGAGCGA TCAGGAAACC AAATACCGCC AACGCTACGT CGATTTGATT GCCAATGAAG AATCGCGCAA TACCTTTATC AAACGCAGCC AAATCATCCA ATCTGTGCGT AATTTTATGG TGGGCGAGCA TTATCTCGAA GTCGAAACCC CGATGATGCA CCCGATTCCC GGCGGCGCGA CAGCGAAACC CTTCGTTACC CACCACAATG CCTTGGACAT TCCGCTCTAC CTGCGCATCG CGCCCGAGCT GTATCTGAAA CGCTTGGTGG TCGGCGGCTT GGAACGCGTG TTTGAAATCA ACCGCAGCTT CCGTAACGAG GGCATGTCCG TGCGCCACAA CCCCGAATTT ACGATGATTG AATTTTACGA AGCCTTCTCC GACTACGAAC GTATGATGCA GATGGCGGAA GACATCATCC GCAACGCATC GCGCACGGTA AACGGCACGG CAAACATCAC TTACAACGGC AAAGAAGTCG ATTTGGAAAG CCCGTTCGAA CGTCTGACCA TCCTCGAAGC CATCAAAAAA TACAATCCGC ACTACACCGA CGAGCAGTTG AACGATGCGG AATGGCTGGC AGCTGAAATC GTCAAACACG GCGAAAGCCT GCCGCCGTCC CCGGGCATCG GCAGCCTGCA ACTCGCCCTG TTTGAAGGTA CCGCCGAGGG CAAACTGTGG AATCCGACCT TTATTGTCGA TTACCCGGTC GAAGTTTCCC CGTTGGCGCG CGCTTCGGAT ACGAAACAAG GTCTGACCGA ACGCTTCGAG CTTTTCGTTG TCGGGCGCGA ACTGGCAAAC GGCTATTCCG AGTTGAACGA CCCCGAAGAC CAAGCCGAAC GCTTCAAATC GCAAGTGGCG CAAAAAGATG CGGGCGACGA CGAAGCCATG CACTACGATG CCGACTACAT CCGCGCGATG GAATTCGGTT TGCCGCCGAC CGGCGGTTGC GGCATCGGCA TCGACCGCTT AGTGATGCTG CTGACCGATT TGCAAACCAT CCGCGATGTG ATTCTGTTTC CGCAGATGCG TCCCGAGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA