NegoA.00263.a
phosphoribosylformylglycinamidine cyclo-ligase
| CENTER ID: | NegoA.00263.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00263.a.B1.GE39967 | full length | 1 | 344 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00263.a.B1.PW37900 | full length | 1 | 344 |
Structures
5VK4
DEPOSITED: 4/20/2017
DETERMINATION: XRay
CLONE: NegoA.00263.a.B1.GE39967
PROTEIN: NegoA.00263.a.B1.PW37900
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.1507 |
| UniProt: | B4RMP6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSLSYRDA GVGIDAGDQL VEKIKPFAKR TMRPEVLGDL GGFGALVEIG KKYQNPVLVS GTDGVGTKLK LAFDWDKHDT VGIDLVAMSV NDILVQGAEP LFFLDYFACG KLDVPRATDV IKGIAQGCEE SGCALIGGET AEMPGMYPVG EYDLAGFAVG VVEKENVITG LSVGAGDMVL GLASNGAHSN GYSLIRKIIE RDNPDLDAEF DNGKTLREAV IAPTRLYVKP ILAALEKFTI KGMAHITGGG ITENVPRVLP KNTVAQIDAE SWELPKLFQW LQKAGNVETQ EMYRTFNCGI GMVVIVAAED ADAVRSFLSG QGETVYRLGC IRERQGNEHQ TQVA
NT Sequence
ATGAGTACTT CATTGAGTTA CCGCGATGCG GGCGTCGGTA TCGACGCAGG CGACCAACTG GTCGAAAAAA TCAAACCGTT TGCCAAACGC ACCATGCGTC CGGAAGTATT GGGGGATTTG GGCGGTTTTG GCGCATTGGT CGAAATCGGC AAAAAATATC AAAACCCCGT ATTGGTCAGC GGTACGGACG GCGTGGGTAC CAAGCTCAAA CTTGCCTTCG ATTGGGACAA ACACGATACG GTGGGCATCG ACCTTGTTGC AATGAGTGTC AACGATATTT TGGTTCAAGG AGCTGAACCA TTGTTTTTCT TGGATTATTT TGCCTGCGGT AAATTGGATG TTCCGCGTGC GACCGATGTC ATCAAAGGCA TTGCCCAAGG TTGCGAAGAA TCCGGCTGCG CCCTGATTGG CGGGGAAACT GCCGAAATGC CGGGTATGTA TCCCGTTGGT GAATACGATT TGGCGGGTTT TGCCGTCGGC GTGGTGGAAA AAGAGAATGT CATTACCGGC CTCAGCGTCG GCGCGGGCGA TATGGTATTG GGTTTGGCTT CCAACGGCGC ACATTCAAAC GGCTATTCCC TTATCCGTAA AATCATCGAA CGCGACAATC CCGATCTGGA TGCCGAGTTT GATAATGGCA AAACTTTACG TGAAGCCGTT ATTGCGCCGA CACGTCTGTA TGTGAAACCG ATTCTTGCCG CTTTGGAAAA ATTTACCATT AAAGGTATGG CGCACATTAC CGGCGGCGGC ATTACCGAAA ACGTGCCGCG CGTGTTGCCT AAAAACACGG TTGCCCAAAT CGATGCCGAA TCGTGGGAAT TGCCCAAGCT CTTCCAATGG CTTCAAAAGG CGGGCAATGT GGAAACCCAA GAAATGTACC GGACCTTTAA CTGCGGCATC GGTATGGTCG TCATCGTTGC TGCCGAAGAT GCCGATGCGG TTCGGTCATT CCTGAGCGGG CAGGGCGAGA CAGTGTACCG TTTGGGTTGC ATCCGAGAGC GTCAAGGGAA CGAGCATCAA ACCCAAGTTG CCTGA
Details for NegoA.00263.a.B1.GE39967
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS TSLSYRDAGV GIDAGDQLVE KIKPFAKRTM RPEVLGDLGG FGALVEIGKK YQNPVLVSGT DGVGTKLKLA FDWDKHDTVG IDLVAMSVND ILVQGAEPLF FLDYFACGKL DVPRATDVIK GIAQGCEESG CALIGGETAE MPGMYPVGEY DLAGFAVGVV EKENVITGLS VGAGDMVLGL ASNGAHSNGY SLIRKIIERD NPDLDAEFDN GKTLREAVIA PTRLYVKPIL AALEKFTIKG MAHITGGGIT ENVPRVLPKN TVAQIDAESW ELPKLFQWLQ KAGNVETQEM YRTFNCGIGM VVIVAAEDAD AVRSFLSGQG ETVYRLGCIR ERQGNEHQTQ VA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta cttcattgag ttaccgcgat gcgggcgtcg gtatcgacgc aggcgaccaa ctggtcgaaa aaatcaaacc gtttgccaaa cgcaccatgc gtccggaagt attgggggat ttgggcggtt ttggcgcatt ggtcgaaatc ggcaaaaaat atcaaaaccc cgtattggtc agcggtacgg acggcgtggg taccaagctc aaacttgcct tcgattggga caaacacgat acggtgggca tcgaccttgt tgcaatgagt gtcaacgata ttttggttca aggagctgaa ccattgtttt tcttggatta ttttgcctgc ggtaaattgg atgttccgcg tgcgaccgat gtcatcaaag gcattgccca aggttgcgaa gaatccggct gcgccctgat tggcggggaa actgccgaaa tgccgggtat gtatcccgtt ggtgaatacg atttggcggg ttttgccgtc ggcgtggtgg aaaaagagaa tgtcattacc ggcctcagcg tcggcgcggg cgatatggta ttgggtttgg cttccaacgg cgcacattca aacggctatt cccttatccg taaaatcatc gaacgcgaca atcccgatct ggatgccgag tttgataatg gcaaaacttt acgtgaagcc gttattgcgc cgacacgtct gtatgtgaaa ccgattcttg ccgctttgga aaaatttacc attaaaggta tggcgcacat taccggcggc ggcattaccg aaaacgtgcc gcgcgtgttg cctaaaaaca cggttgccca aatcgatgcc gaatcgtggg aattgcccaa gctcttccaa tggcttcaaa aggcgggcaa tgtggaaacc caagaaatgt accggacctt taactgcggc atcggtatgg tcgtcatcgt tgctgccgaa gatgccgatg cggttcggtc attcctgagc gggcagggcg agacagtgta ccgtttgggt tgcatccgag agcgtcaagg gaacgagcat caaacccaag ttgcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for NegoA.00263.a.B1.PW37900
| PURIFICATION DATe: | 5/3/2016 |
| CONCENTRATION: | 36.5mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS TSLSYRDAGV GIDAGDQLVE KIKPFAKRTM RPEVLGDLGG FGALVEIGKK YQNPVLVSGT DGVGTKLKLA FDWDKHDTVG IDLVAMSVND ILVQGAEPLF FLDYFACGKL DVPRATDVIK GIAQGCEESG CALIGGETAE MPGMYPVGEY DLAGFAVGVV EKENVITGLS VGAGDMVLGL ASNGAHSNGY SLIRKIIERD NPDLDAEFDN GKTLREAVIA PTRLYVKPIL AALEKFTIKG MAHITGGGIT ENVPRVLPKN TVAQIDAESW ELPKLFQWLQ KAGNVETQEM YRTFNCGIGM VVIVAAEDAD AVRSFLSGQG ETVYRLGCIR ERQGNEHQTQ VA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA CTTCATTGAG TTACCGCGAT GCGGGCGTCG GTATCGACGC AGGCGACCAA CTGGTCGAAA AAATCAAACC GTTTGCCAAA CGCACCATGC GTCCGGAAGT ATTGGGGGAT TTGGGCGGTT TTGGCGCATT GGTCGAAATC GGCAAAAAAT ATCAAAACCC CGTATTGGTC AGCGGTACGG ACGGCGTGGG TACCAAGCTC AAACTTGCCT TCGATTGGGA CAAACACGAT ACGGTGGGCA TCGACCTTGT TGCAATGAGT GTCAACGATA TTTTGGTTCA AGGAGCTGAA CCATTGTTTT TCTTGGATTA TTTTGCCTGC GGTAAATTGG ATGTTCCGCG TGCGACCGAT GTCATCAAAG GCATTGCCCA AGGTTGCGAA GAATCCGGCT GCGCCCTGAT TGGCGGGGAA ACTGCCGAAA TGCCGGGTAT GTATCCCGTT GGTGAATACG ATTTGGCGGG TTTTGCCGTC GGCGTGGTGG AAAAAGAGAA TGTCATTACC GGCCTCAGCG TCGGCGCGGG CGATATGGTA TTGGGTTTGG CTTCCAACGG CGCACATTCA AACGGCTATT CCCTTATCCG TAAAATCATC GAACGCGACA ATCCCGATCT GGATGCCGAG TTTGATAATG GCAAAACTTT ACGTGAAGCC GTTATTGCGC CGACACGTCT GTATGTGAAA CCGATTCTTG CCGCTTTGGA AAAATTTACC ATTAAAGGTA TGGCGCACAT TACCGGCGGC GGCATTACCG AAAACGTGCC GCGCGTGTTG CCTAAAAACA CGGTTGCCCA AATCGATGCC GAATCGTGGG AATTGCCCAA GCTCTTCCAA TGGCTTCAAA AGGCGGGCAA TGTGGAAACC CAAGAAATGT ACCGGACCTT TAACTGCGGC ATCGGTATGG TCGTCATCGT TGCTGCCGAA GATGCCGATG CGGTTCGGTC ATTCCTGAGC GGGCAGGGCG AGACAGTGTA CCGTTTGGGT TGCATCCGAG AGCGTCAAGG GAACGAGCAT CAAACCCAAG TTGCCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT