NegoA.00222.a

signal recognition particle protein

CENTER ID:	NegoA.00222.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00222.a.B1.GE39965	full length		1	456

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
de-novo with evolutionary constraints	ModelArchive_ma-u7vmd

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.2243
UniProt:	B4RPC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDNLTGRFS NVFKNIRGQA KLTEDNIKEA LREVRLALLE ADVALPVVKE FVNNVKEKAL GQEVAGSLTP DQAFIGVVNQ ALVELMGKEN KTLDLSVSPP AIILMAGLQG AGKTTTVGKL ARLLKNDQKK KVLVVSADVY RPAAIEQLRL LAEQVGVDFF PSDTNQKPVE IATAAVDYAK KHFYDVLMVD TAGRLAIDEE MMNEIKALHA VVNPVETLFV IDAMLGQDAV NTAQAFNEAL PLNGVVLTKM DGDSRGGAAL SVRHVTGKPI KFIGVGEKIN GLEPFHPDRL ASRILGMGDV LTLIEDVQKG IDEEAAAKMA KKLQKGKGFD LNDFKEQIQQ MRNMGGLENL MSKMPGELGQ ISKQIPEGTA EKAMGKVEAI INSMTPKERA NPALLKAGRK RRIAMGAGTT VQEVNKLLKQ FEQMQQMMKM FSGNGLGKLM RLAKGMKGMK GMFPGL

NT Sequence

ATGTTAGACA ATTTAACCGG CCGCTTCAGC AATGTCTTCA AAAACATCCG GGGGCAGGCC AAACTGACCG AAGACAATAT TAAAGAAGCC TTGCGCGAAG TCCGACTCGC CCTGCTTGAG GCGGATGTCG CCCTGCCTGT CGTCAAAGAG TTCGTCAACA ACGTCAAAGA AAAGGCCCTC GGTCAGGAAG TGGCGGGCAG CCTGACGCCT GACCAAGCCT TTATCGGCGT GGTCAACCAA GCCTTAGTCG AACTGATGGG CAAGGAAAAC AAAACACTGG ATTTATCGGT TTCACCGCCT GCCATCATTT TGATGGCCGG TTTGCAGGGT GCCGGTAAGA CAACAACCGT CGGCAAACTC GCCCGCCTGC TGAAAAACGA TCAGAAGAAA AAAGTTTTGG TGGTATCCGC CGACGTTTAC CGCCCTGCCG CGATTGAACA GCTGCGCCTG TTGGCCGAAC AGGTCGGCGT AGATTTTTTC CCATCCGATA CCAACCAAAA ACCGGTTGAG ATTGCAACTG CCGCCGTTGA TTACGCCAAA AAACATTTTT ACGATGTATT GATGGTCGAT ACCGCCGGCC GTTTGGCAAT CGATGAAGAG ATGATGAACG AAATCAAAGC GCTTCATGCG GTGGTCAACC CGGTTGAAAC TTTGTTCGTT ATCGATGCCA TGCTGGGTCA GGATGCGGTA AATACCGCTC AGGCATTTAA TGAAGCCCTG CCGCTGAACG GCGTCGTATT GACCAAGATG GACGGCGACT CGCGCGGCGG TGCGGCATTG TCCGTACGCC ATGTAACCGG CAAACCGATT AAATTTATCG GCGTCGGCGA GAAAATCAAC GGCCTCGAAC CTTTCCACCC CGACCGTCTT GCCAGCCGTA TCCTCGGCAT GGGCGACGTA TTGACCCTGA TTGAAGACGT TCAAAAAGGT ATTGATGAAG AAGCCGCCGC CAAAATGGCG AAAAAGCTGC AAAAAGGCAA AGGCTTTGAC CTCAATGATT TTAAAGAACA AATCCAGCAA ATGCGCAATA TGGGCGGTTT GGAAAACCTG ATGTCGAAAA TGCCGGGCGA ACTGGGTCAA ATATCGAAAC AAATCCCCGA AGGTACGGCT GAAAAAGCGA TGGGCAAAGT AGAAGCCATC ATCAACTCGA TGACCCCTAA AGAACGCGCC AACCCTGCCC TGCTCAAAGC CGGCCGCAAA CGCCGTATTG CGATGGGCGC GGGCACGACC GTGCAGGAAG TGAACAAATT GCTCAAACAG TTTGAACAAA TGCAGCAGAT GATGAAGATG TTCAGCGGCA ACGGTTTGGG CAAACTGATG CGCTTGGCCA AAGGCATGAA GGGAATGAAG GGAATGTTTC CCGGTCTTTA A

Details for NegoA.00222.a.B1.GE39965

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	51kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML DNLTGRFSNV FKNIRGQAKL TEDNIKEALR EVRLALLEAD VALPVVKEFV NNVKEKALGQ EVAGSLTPDQ AFIGVVNQAL VELMGKENKT LDLSVSPPAI ILMAGLQGAG KTTTVGKLAR LLKNDQKKKV LVVSADVYRP AAIEQLRLLA EQVGVDFFPS DTNQKPVEIA TAAVDYAKKH FYDVLMVDTA GRLAIDEEMM NEIKALHAVV NPVETLFVID AMLGQDAVNT AQAFNEALPL NGVVLTKMDG DSRGGAALSV RHVTGKPIKF IGVGEKINGL EPFHPDRLAS RILGMGDVLT LIEDVQKGID EEAAAKMAKK LQKGKGFDLN DFKEQIQQMR NMGGLENLMS KMPGELGQIS KQIPEGTAEK AMGKVEAIIN SMTPKERANP ALLKAGRKRR IAMGAGTTVQ EVNKLLKQFE QMQQMMKMFS GNGLGKLMRL AKGMKGI

Validated NT Sequence

ttattccctt catgcctttg gccaagcgca tcagtttgcc caaaccgttg ccgctgaaca tcttcatcat ctgctgcatt tgttcaaact gtttgagcaa tttgttcact tcctgcacgg tcgtgcccgc gcccatcgca atacggcgtt tgcggccggc tttgagcagg gcagggttgg cgcgttcttt aggggtcatc gagttgatga tggcttctac tttgcccatc gctttttcag ccgtaccttc ggggatttgt ttcgatattt gacccagttc gcccggcatt ttcgacatca ggttttccaa accgcccata ttgcgcattt gctggatttg ttctttaaaa tcattgaggt caaagccttt gcctttttgc agctttttcg ccattttggc ggcggcttct tcatcaatac ctttttgaac gtcttcaatc agggtcaata cgtcgcccat gccgaggata cggctggcaa gacggtcggg gtggaaaggt tcgaggccgt tgattttctc gccgacgccg ataaatttaa tcggtttgcc ggttacatgg cgtacggaca atgccgcacc gccgcgcgag tcgccgtcca tcttggtcaa tacgacgccg ttcagcggca gggcttcatt aaatgcctga gcggtattta ccgcatcctg acccagcatg gcatcgataa cgaacaaagt ttcaaccggg ttgaccaccg catgaagcgc tttgatttcg ttcatcatct cttcatcgat tgccaaacgg ccggcggtat cgaccatcaa tacatcgtaa aaatgttttt tggcgtaatc aacggcggca gttgcaatct caaccggttt ttggttggta tcggatggga aaaaatctac gccgacctgt tcggccaaca ggcgcagctg ttcaatcgcg gcagggcggt aaacgtcggc ggataccacc aaaacttttt tcttctgatc gtttttcagc aggcgggcga gtttgccgac ggttgttgtc ttaccggcac cctgcaaacc ggccatcaaa atgatggcag gcggtgaaac cgataaatcc agtgttttgt tttccttgcc catcagttcg actaaggctt ggttgaccac gccgataaag gcttggtcag gcgtcaggct gcccgccact tcctgaccga gggccttttc tttgacgttg ttgacgaact ctttgacgac aggcagggcg acatccgcct caagcagggc gagtcggact tcgcgcaagg cttctttaat attgtcttcg gtcagtttgg cctgcccccg gatgtttttg aagacattgc tgaagcggcc ggttaaattg tctaacatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag acaatttaac cggccgcttc agcaatgtct tcaaaaacat ccgggggcag gccaaactga ccgaagacaa tattaaagaa gccttgcgcg aagtccgact cgccctgctt gaggcggatg tcgccctgcc tgtcgtcaaa gagttcgtca acaacgtcaa agaaaaggcc ctcggtcagg aagtggcggg cagcctgacg cctgaccaag cctttatcgg cgtggtcaac caagccttag tcgaactgat gggcaaggaa aacaaaacac tggatttatc ggtttcaccg cctgccatca ttttgatggc cggtttgcag ggtgccggta agacaacaac cgtcggcaaa ctcgcccgcc tgctgaaaaa cgatcagaag aaaaaagttt tggtggtatc cgccgacgtt taccgccctg ccgcgattga acagctgcgc ctgttggccg aacaggtcgg cgtagatttt ttcccatccg ataccaacca aaaaccggtt gagattgcaa ctgccgccgt tgattacgcc aaaaaacatt tttacgatgt attgatggtc gataccgccg gccgtttggc aatcgatgaa gagatgatga acgaaatcaa agcgcttcat gcggtggtca acccggttga aactttgttc gttatcgatg ccatgctggg tcaggatgcg gtaaataccg ctcaggcatt taatgaagcc ctgccgctga acggcgtcgt attgaccaag atggacggcg actcgcgcgg cggtgcggca ttgtccgtac gccatgtaac cggcaaaccg attaaattta tcggcgtcgg cgagaaaatc aacggcctcg aacctttcca ccccgaccgt cttgccagcc gtatcctcgg catgggcgac gtattgaccc tgattgaaga cgttcaaaaa ggtattgatg aagaagccgc cgccaaaatg gcgaaaaagc tgcaaaaagg caaaggcttt gacctcaatg attttaaaga acaaatccag caaatgcgca atatgggcgg tttggaaaac ctgatgtcga aaatgccggg cgaactgggt caaatatcga aacaaatccc cgaaggtacg gctgaaaaag cgatgggcaa agtagaagcc atcatcaact cgatgacccc taaagaacgc gccaaccctg ccctgctcaa agccggccgc aaacgccgta ttgcgatggg cgcgggcacg accgtgcagg aagtgaacaa attgctcaaa cagtttgaac aaatgcagca gatgatgaag atgttcagcg gcaacggttt gggcaaactg atgcgcttgg ccaaaggcat gaagggaatg aagggaatgt ttcccggtct ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat