NegoA.00206.a

phosphopantothenoylcysteine decarboxylase/phosphopantothenate-cysteine ligase

CENTER ID: NegoA.00206.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.00206.a.B1.GE39964 full length 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.1629
UniProt: B4RN18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKHILLGVT GSIAAYKSCE LVRLLKKQGH SVTVVMSRSA TEFVSPLTFQ ALSGNPVLTD THGGNGSNGM EHINLTRNAD VFLIAPASMN TVAKICNGVA DNLLTNLAAA RKCPLAIAPA MNVEMWLNPA NQRNIAQLVS DGITVYMPGL GEQACRENGM GRMPEPAELL DLLPDLWTPK ILRDKKILIT AGATFEAIDP VRGITNTSSG KMGVALARAC RAAGAEISLI HGQLQTTLPF GISDTVQAVS AEDMHRAVHR LIEKQDAFIS VAAVSDYKVK NRSTQKFKKD KNAKPLSIEL DENPDTLASI ASLPNPPFCI GFAAETENVM AYAREKRIKK KIPVIVANDV SIAMGKTTNQ IVIIDDDAEL SFPETSKDEA AMRIVERLAV YLNK
NT Sequence
ATGGGCAAAC ATATTCTTTT AGGTGTAACG GGCAGTATTG CGGCGTATAA GTCTTGCGAG TTGGTGCGAC TGCTGAAAAA ACAGGGGCAT TCGGTTACGG TGGTTATGAG CCGCTCGGCA ACTGAATTTG TTTCTCCGCT GACTTTTCAG GCTTTGAGCG GCAATCCCGT TCTGACCGAC ACGCACGGCG GCAACGGTTC AAACGGTATG GAACATATCA ACCTGACCCG GAATGCGGAT GTTTTTCTGA TTGCGCCGGC AAGTATGAAT ACCGTGGCAA AAATCTGTAA CGGCGTGGCA GATAACCTAC TGACCAATCT GGCAGCCGCA CGGAAATGCC CTCTTGCCAT CGCTCCCGCG ATGAATGTGG AAATGTGGCT GAATCCTGCC AACCAACGGA ATATCGCACA ACTGGTTTCA GACGGCATTA CTGTCTATAT GCCGGGCTTG GGCGAACAGG CTTGCAGAGA AAATGGTATG GGAAGGATGC CGGAACCTGC CGAATTGCTG GATTTGCTTC CAGACTTGTG GACACCGAAA ATTTTAAGGG ACAAGAAAAT CTTGATTACC GCAGGTGCGA CATTTGAAGC CATTGACCCT GTCAGGGGCA TCACAAATAC CTCCAGCGGA AAAATGGGCG TAGCTTTGGC GCGGGCGTGC CGTGCCGCCG GTGCGGAAAT CAGCCTGATT CACGGACAGC TTCAAACCAC GCTGCCTTTC GGCATATCCG ATACGGTTCA AGCCGTCAGT GCCGAAGATA TGCATCGCGC AGTACATCGT TTGATTGAAA AACAAGATGC TTTTATTTCT GTTGCCGCCG TCTCAGACTA TAAGGTTAAA AACAGGAGTA CGCAAAAATT CAAAAAAGAT AAAAATGCCA AACCGTTATC CATCGAATTG GATGAGAACC CCGATACTTT GGCTTCTATT GCCTCATTGC CGAACCCGCC GTTCTGCATC GGTTTTGCCG CTGAAACGGA GAATGTAATG GCATATGCGC GGGAAAAACG TATTAAGAAA AAAATACCGG TGATAGTTGC CAATGATGTT TCAATCGCGA TGGGCAAGAC AACCAACCAA ATTGTTATCA TAGATGATGA TGCGGAGTTG TCTTTCCCCG AAACAAGTAA AGATGAAGCG GCAATGCGGA TTGTTGAAAG GCTTGCCGTA TATTTGAACA AATAA
Details for NegoA.00206.a.B1.GE39964
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG KHILLGVTGS IAAYKSCELV RLLKKQGHSV TVVMSRSATE FVSPLTFQAL SGNPVLTDTH GGNGSNGMEH INLTRNADVF LIAPASMNTV AKICNGVADN LLTNLAAARK CPLAIAPAMN VEMWLNPANQ RNIAQLVSDG ITVYMPGLGE QACRENGMGR MPEPAELLDL LPDLWTPKIL RDKKILITAG ATFEAIDPVR GITNTSSGKM GVALARACRA AGAEISLIHG QLQTTLPFGI SDTVQAVSAE DMHRAVHRLI EKQDAFISVA AVSDYKVKNR STQKFKKDKN AKPLSIELDE NPDTLASIAS LPNPPFCIGF AAETENVMAY AREKRIKKKI PVIVANDVSI AMGKTTNQIV IIDDDAELSF PETSKDEAAM RIVERLAVYL NK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca aacatattct tttaggtgta acgggcagta ttgcggcgta taagtcttgc gagttggtgc gactgctgaa aaaacagggg cattcggtta cggtggttat gagccgctcg gcaactgaat ttgtttctcc gctgactttt caggctttga gcggcaatcc cgttctgacc gacacgcacg gcggcaacgg ttcaaacggt atggaacata tcaacctgac ccggaatgcg gatgtttttc tgattgcgcc ggcaagtatg aataccgtgg caaaaatctg taacggcgtg gcagataacc tactgaccaa tctggcagcc gcacggaaat gccctcttgc catcgctccc gcgatgaatg tggaaatgtg gctgaatcct gccaaccaac ggaatatcgc acaactggtt tcagacggca ttactgtcta tatgccgggc ttgggcgaac aggcttgcag agaaaatggt atgggaagga tgccggaacc tgccgaattg ctggatttgc ttccagactt gtggacaccg aaaattttaa gggacaagaa aatcttgatt accgcaggtg cgacatttga agccattgac cctgtcaggg gcatcacaaa tacctccagc ggaaaaatgg gcgtagcttt ggcgcgggcg tgccgtgccg ccggtgcgga aatcagcctg attcacggac agcttcaaac cacgctgcct ttcggcatat ccgatacggt tcaagccgtc agtgccgaag atatgcatcg cgcagtacat cgtttgattg aaaaacaaga tgcttttatt tctgttgccg ccgtctcaga ctataaggtt aaaaacagga gtacgcaaaa attcaaaaaa gataaaaatg ccaaaccgtt atccatcgaa ttggatgaga accccgatac tttggcttct attgcctcat tgccgaaccc gccgttctgc atcggttttg ccgctgaaac ggagaatgta atggcatatg cgcgggaaaa acgtattaag aaaaaaatac cggtgatagt tgccaatgat gtttcaatcg cgatgggcaa gacaaccaac caaattgtta tcatagatga tgatgcggag ttgtctttcc ccgaaacaag taaagatgaa gcggcaatgc ggattgttga aaggcttgcc gtatatttga acaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat