NegoA.00177.a

chaperone dnaK

CENTER ID:	NegoA.00177.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00177.a.B1.GE39961	full length		1	642

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00177.a.B1.PW37907	full length		1	642

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_85035

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.1797
UniProt:	B4RP29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKVIGIDLG TTNSCLAISE NGQTKVIENA EGARTTPSII AYLDGGEILV GAPAKRQAVT NAKNTIYAAK RLIGHKFEDK EVQRDIESMP FEIIKADNGD AWVKAQGKEL SPPQISAEVL RKMKEAAEAY LGEKVTEAVI TVPAYFNDSQ RQATKDAGRI AGLDVKRIIN EPTAAALAFG MDKGDNKDRK IAVYDLGGGT FDISIIEIAN LDGDKQFEVL ATNGDTFLGG EDFDQRLIDY IIDEFKKEQG IDLKQDVMAL QRLKEAAEKA KIELSSGQQT EINLPYITMD ATGPKHLAMK ITRAKFESLV EDLIARSIEP CRTALKDAGL STGDIDDVIL VGGQSRMPKV QEAVKDFFGK EPRKDVNPDE AVAVGAAIQG EVLSGGRSDV LLLDVTPLSL GIETMGGVMT KLIQKNTTIP TKASQVFSTA EDNQSAVTIH VLQGERERAS ANKSLGQFNL GDIAPAPRGM PQIEVTFDID ANGILHVSAK DKGTGKAANI TIQGSSGLSE EEIERMVKDA EANAEEDKKL TELVASRNQA EALIHSVKKS LADYGDKLDA AEKEKIEAAL KEAEEAVKGD DKTAIDAKAE ALGTASQKLG EMVYAQAQAE AQAGEGAQAN ASAKKDDDVV DADFEEVKDD KK

NT Sequence

ATGGCAAAAG TAATCGGTAT CGACTTAGGT ACAACCAACT CTTGTTTGGC CATTTCCGAA AACGGTCAAA CCAAAGTCAT CGAAAACGCA GAAGGCGCAC GCACCACGCC GTCCATTATC GCTTATTTGG ACGGCGGCGA AATCCTCGTC GGCGCGCCTG CCAAACGCCA AGCGGTAACC AACGCCAAAA ACACTATTTA CGCCGCCAAA CGTTTGATCG GTCACAAATT TGAAGACAAA GAAGTCCAAC GCGACATCGA ATCTATGCCT TTCGAAATCA TCAAAGCCGA CAACGGCGAC GCATGGGTAA AAGCACAAGG CAAAGAGCTG TCTCCTCCTC AAATTTCCGC AGAAGTCCTG CGTAAAATGA AAGAAGCCGC CGAAGCTTAC TTGGGCGAAA AAGTAACCGA AGCCGTGATT ACCGTCCCTG CCTACTTCAA CGACAGCCAA CGTCAAGCCA CCAAAGACGC AGGCCGTATC GCCGGTTTGG ACGTAAAACG CATCATCAAC GAGCCGACCG CAGCCGCTTT GGCATTCGGT ATGGACAAAG GCGACAACAA AGACCGCAAA ATAGCCGTAT ATGACTTGGG CGGCGGTACC TTCGATATTT CCATCATCGA AATCGCCAAC CTCGACGGCG ACAAACAATT TGAAGTATTG GCTACCAACG GCGATACTTT CTTGGGCGGT GAAGACTTCG ACCAACGCTT GATTGACTAC ATCATTGACG AGTTTAAAAA AGAACAAGGC ATTGATTTGA AACAAGACGT AATGGCTCTG CAACGTCTGA AAGAAGCTGC CGAAAAAGCC AAAATCGAAT TGTCCAGCGG CCAGCAAACC GAAATCAACC TGCCGTACAT TACCATGGAC GCAACCGGCC CGAAACACTT GGCAATGAAA ATTACCCGCG CCAAATTCGA GAGCCTGGTT GAAGACCTGA TTGCCCGCTC TATCGAGCCT TGCCGCACCG CATTGAAAGA TGCCGGCTTG AGCACCGGCG ACATCGACGA CGTGATTTTG GTCGGCGGTC AGTCCCGTAT GCCGAAAGTA CAAGAAGCCG TTAAAGACTT CTTCGGCAAA GAACCGCGCA AAGACGTGAA CCCTGACGAA GCCGTTGCCG TAGGTGCAGC GATTCAAGGC GAAGTATTGA GCGGCGGCCG CAGCGACGTA TTGCTGCTGG ACGTAACCCC TCTGTCCTTG GGTATCGAAA CCATGGGTGG TGTGATGACC AAGCTGATTC AAAAGAACAC TACTATTCCG ACTAAAGCGT CTCAAGTGTT CTCTACTGCC GAAGACAACC AAAGCGCAGT AACCATCCAC GTACTGCAAG GCGAACGCGA ACGCGCTTCT GCCAACAAAT CTTTAGGTCA ATTCAACTTG GGCGACATCG CACCCGCACC ACGCGGTATG CCGCAAATTG AAGTTACTTT CGACATCGAC GCCAATGGTA TCTTGCACGT TTCCGCCAAA GACAAAGGCA CTGGCAAAGC GGCCAACATT ACCATCCAAG GTTCTTCAGG TTTGAGCGAA GAAGAAATCG AACGCATGGT GAAAGATGCC GAAGCCAATG CCGAGGAAGA TAAAAAGCTG ACTGAATTGG TCGCTTCCCG CAACCAAGCC GAAGCCCTGA TTCACTCCGT GAAAAAATCT TTGGCGGACT ACGGCGACAA ACTCGACGCT GCCGAGAAAG AAAAAATCGA AGCCGCGCTG AAAGAAGCCG AAGAAGCCGT GAAAGGCGAC GACAAAACCG CCATCGATGC CAAAGCCGAA GCACTGGGCA CAGCCAGCCA AAAACTGGGC GAAATGGTTT ACGCGCAAGC GCAAGCCGAA GCCCAAGCCG GCGAGGGCGC ACAAGCCAAT GCTTCTGCAA AGAAAGACGA TGATGTCGTA GATGCCGACT TTGAAGAAGT AAAAGACGAC AAAAAATAA

Details for NegoA.00177.a.B1.GE39961

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	70kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA KVIGIDLGTT NSCLAISENG QTKVIENAEG ARTTPSIIAY LDGGEILVGA PAKRQAVTNA KNTIYAAKRL IGHKFEDKEV QRDIESMPFE IIKADNGDAW VKAQGKELSP PQISAEVLRK MKEAAEAYLG EKVTEAVITV PAYFNDSQRQ ATKDAGRIAG LDVKRIINEP TAAALAFGMD KGDNKDRKIA VYDLGGGTFD ISIIEIANLD GDKQFEVLAT NGDTFLGGED FDQRLIDYII DEFKKEQGID LKQDVMALQR LKEAAEKAKI ELSSGQQTEI NLPYITMDAT GPKHLAMKIT RAKFESLVED LIARSIEPCR TALKDAGLST GDIDDVILVG GQSRMPKVQE AVKDFFGKEP RKDVNPDEAV AVGAAIQGEV LSGGRSDVLL LDVTPLSLGI ETMGGVMTKL IQKNTTIPTK ASQVFSTAED NQSAVTIHVL QGERERASAN KSLGQFNLGD IAPAPRGMPQ IEVTFDIDAN GILHVSAKDK GTGKAANITI QGSSGLSEEE IERMVKDAEA NAEEDKKLTE LVASRNQAEA LIHSVKKSLA DYGDKLDAAE KEKIEAALKE AEEAVKGDDK TAIDAKAEAL GTASQKLGEM VYAQAQAEAQ AGEGAQANAS AKKDDDVVDA DFEEVKDDKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa aagtaatcgg tatcgactta ggtacaacca actcttgttt ggccatttcc gaaaacggtc aaaccaaagt catcgaaaac gcagaaggcg cacgcaccac gccgtccatt atcgcttatt tggacggcgg cgaaatcctc gtcggcgcgc ctgccaaacg ccaagcggta accaacgcca aaaacactat ttacgccgcc aaacgtttga tcggtcacaa atttgaagac aaagaagtcc aacgcgacat cgaatctatg cctttcgaaa tcatcaaagc cgacaacggc gacgcatggg taaaagcaca aggcaaagag ctgtctcctc ctcaaatttc cgcagaagtc ctgcgtaaaa tgaaagaagc cgccgaagct tacttgggcg aaaaagtaac cgaagccgtg attaccgtcc ctgcctactt caacgacagc caacgtcaag ccaccaaaga cgcaggccgt atcgccggtt tggacgtaaa acgcatcatc aacgagccga ccgcagccgc tttggcattc ggtatggaca aaggcgacaa caaagaccgc aaaatagccg tatatgactt gggcggcggt accttcgata tttccatcat cgaaatcgcc aacctcgacg gcgacaaaca atttgaagta ttggctacca acggcgatac tttcttgggc ggtgaagact tcgaccaacg cttgattgac tacatcattg acgagtttaa aaaagaacaa ggcattgatt tgaaacaaga cgtaatggct ctgcaacgtc tgaaagaagc tgccgaaaaa gccaaaatcg aattgtccag cggccagcaa accgaaatca acctgccgta cattaccatg gacgcaaccg gcccgaaaca cttggcaatg aaaattaccc gcgccaaatt cgagagcctg gttgaagacc tgattgcccg ctctatcgag ccttgccgca ccgcattgaa agatgccggc ttgagcaccg gcgacatcga cgacgtgatt ttggtcggcg gtcagtcccg tatgccgaaa gtacaagaag ccgttaaaga cttcttcggc aaagaaccgc gcaaagacgt gaaccctgac gaagccgttg ccgtaggtgc agcgattcaa ggcgaagtat tgagcggcgg ccgcagcgac gtattgctgc tggacgtaac ccctctgtcc ttgggtatcg aaaccatggg tggtgtgatg accaagctga ttcaaaagaa cactactatt ccgactaaag cgtctcaagt gttctctact gccgaagaca accaaagcgc agtaaccatc cacgtactgc aaggcgaacg cgaacgcgct tctgccaaca aatctttagg tcaattcaac ttgggcgaca tcgcacccgc accacgcggt atgccgcaaa ttgaagttac tttcgacatc gacgccaatg gtatcttgca cgtttccgcc aaagacaaag gcactggcaa agcggccaac attaccatcc aaggttcttc aggtttgagc gaagaagaaa tcgaacgcat ggtgaaagat gccgaagcca atgccgagga agataaaaag ctgactgaat tggtcgcttc ccgcaaccaa gccgaagccc tgattcactc cgtgaaaaaa tctttggcgg actacggcga caaactcgac gctgccgaga aagaaaaaat cgaagccgcg ctgaaagaag ccgaagaagc cgtgaaaggc gacgacaaaa ccgccatcga tgccaaagcc gaagcactgg gcacagccag ccaaaaactg ggcgaaatgg tttacgcgca agcgcaagcc gaagcccaag ccggcgaggg cgcacaagcc aatgcttctg caaagaaaga cgatgatgtc gtagatgccg actttgaaga agtaaaagac gacaaaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for NegoA.00177.a.B1.PW37907

PURIFICATION DATe:	5/11/2016
CONCENTRATION:	37mg/ml
OBSERVED MW:	70kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA AAGTAATCGG TATCGACTTA GGTACAACCA ACTCTTGTTT GGCCATTTCC GAAAACGGTC AAACCAAAGT CATCGAAAAC GCAGAAGGCG CACGCACCAC GCCGTCCATT ATCGCTTATT TGGACGGCGG CGAAATCCTC GTCGGCGCGC CTGCCAAACG CCAAGCGGTA ACCAACGCCA AAAACACTAT TTACGCCGCC AAACGTTTGA TCGGTCACAA ATTTGAAGAC AAAGAAGTCC AACGCGACAT CGAATCTATG CCTTTCGAAA TCATCAAAGC CGACAACGGC GACGCATGGG TAAAAGCACA AGGCAAAGAG CTGTCTCCTC CTCAAATTTC CGCAGAAGTC CTGCGTAAAA TGAAAGAAGC CGCCGAAGCT TACTTGGGCG AAAAAGTAAC CGAAGCCGTG ATTACCGTCC CTGCCTACTT CAACGACAGC CAACGTCAAG CCACCAAAGA CGCAGGCCGT ATCGCCGGTT TGGACGTAAA ACGCATCATC AACGAGCCGA CCGCAGCCGC TTTGGCATTC GGTATGGACA AAGGCGACAA CAAAGACCGC AAAATAGCCG TATATGACTT GGGCGGCGGT ACCTTCGATA TTTCCATCAT CGAAATCGCC AACCTCGACG GCGACAAACA ATTTGAAGTA TTGGCTACCA ACGGCGATAC TTTCTTGGGC GGTGAAGACT TCGACCAACG CTTGATTGAC TACATCATTG ACGAGTTTAA AAAAGAACAA GGCATTGATT TGAAACAAGA CGTAATGGCT CTGCAACGTC TGAAAGAAGC TGCCGAAAAA GCCAAAATCG AATTGTCCAG CGGCCAGCAA ACCGAAATCA ACCTGCCGTA CATTACCATG GACGCAACCG GCCCGAAACA CTTGGCAATG AAAATTACCC GCGCCAAATT CGAGAGCCTG GTTGAAGACC TGATTGCCCG CTCTATCGAG CCTTGCCGCA CCGCATTGAA AGATGCCGGC TTGAGCACCG GCGACATCGA CGACGTGATT TTGGTCGGCG GTCAGTCCCG TATGCCGAAA GTACAAGAAG CCGTTAAAGA CTTCTTCGGC AAAGAACCGC GCAAAGACGT GAACCCTGAC GAAGCCGTTG CCGTAGGTGC AGCGATTCAA GGCGAAGTAT TGAGCGGCGG CCGCAGCGAC GTATTGCTGC TGGACGTAAC CCCTCTGTCC TTGGGTATCG AAACCATGGG TGGTGTGATG ACCAAGCTGA TTCAAAAGAA CACTACTATT CCGACTAAAG CGTCTCAAGT GTTCTCTACT GCCGAAGACA ACCAAAGCGC AGTAACCATC CACGTACTGC AAGGCGAACG CGAACGCGCT TCTGCCAACA AATCTTTAGG TCAATTCAAC TTGGGCGACA TCGCACCCGC ACCACGCGGT ATGCCGCAAA TTGAAGTTAC TTTCGACATC GACGCCAATG GTATCTTGCA CGTTTCCGCC AAAGACAAAG GCACTGGCAA AGCGGCCAAC ATTACCATCC AAGGTTCTTC AGGTTTGAGC GAAGAAGAAA TCGAACGCAT GGTGAAAGAT GCCGAAGCCA ATGCCGAGGA AGATAAAAAG CTGACTGAAT TGGTCGCTTC CCGCAACCAA GCCGAAGCCC TGATTCACTC CGTGAAAAAA TCTTTGGCGG ACTACGGCGA CAAACTCGAC GCTGCCGAGA AAGAAAAAAT CGAAGCCGCG CTGAAAGAAG CCGAAGAAGC CGTGAAAGGC GACGACAAAA CCGCCATCGA TGCCAAAGCC GAAGCACTGG GCACAGCCAG CCAAAAACTG GGCGAAATGG TTTACGCGCA AGCGCAAGCC GAAGCCCAAG CCGGCGAGGG CGCACAAGCC AATGCTTCTG CAAAGAAAGA CGATGATGTC GTAGATGCCG ACTTTGAAGA AGTAAAAGAC GACAAAAAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT