NegoA.00176.a
RsmE family RNA methyltransferase
| CENTER ID: | NegoA.00176.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00176.a.B1.GE39960 | full length | 1 | 241 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00176.a.B1.PW37905 | full length | 1 | 241 |
Structures
5VM8
DEPOSITED: 4/26/2017
DETERMINATION: XRay
CLONE: NegoA.00176.a.B1.GE39960
PROTEIN: NegoA.00176.a.B1.PW37905
External Resources
| RESOURCE | REFERENCE ID |
|---|
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRFYLPENL SVGQTVALPD NIVRHLNVLR VRPNENITLF DGKGKAHTAR LTVLEKHRAE AEILHEDTTD NESPLNITLI QSISSGDRMD FTLQKSVELG VTAIQPVISE RCIVRLDGER AAKRLARWQE IVISACEQSG RNTVPPVLPI IGYREALDKM PSESTRLIMS INRARKLGDI RHPSGAIVFM VGPEGGWTEQ EEQQAFEAGF QAVTLGKRIL RTETAPLAAI AAMQTLWGDF T
NT Sequence
ATGCCCAGAT TCTACCTGCC CGAAAACCTT TCCGTCGGAC AAACCGTCGC CCTGCCCGAC AACATCGTCC GCCACCTCAA CGTCCTGCGC GTCCGCCCCA ACGAAAACAT CACCCTCTTC GACGGCAAAG GCAAGGCACA TACCGCACGG CTGACCGTTT TGGAAAAACA CCGCGCCGAA GCCGAAATCC TGCACGAAGA CACAACCGAC AACGAGTCCC CGCTCAACAT CACGCTCATC CAATCCATTT CCTCCGGCGA TCGCATGGAT TTCACCCTGC AAAAAAGCGT CGAACTCGGC GTAACCGCCA TACAGCCCGT CATCAGCGAA CGCTGCATCG TCCGCCTCGA CGGAGAACGC GCCGCCAAAC GCCTCGCACG CTGGCAGGAA ATCGTCATCT CCGCGTGCGA ACAAAGCGGC AGGAACACCG TTCCCCCCGT ACTGCCCATC ATCGGCTACC GTGAAGCACT CGACAAAATG CCGTCTGAAA GCACCAGGCT GATTATGAGC ATCAACCGCG CCCGCAAACT CGGCGACATC CGCCACCCGT CCGGCGCAAT CGTCTTTATG GTCGGGCCCG AAGGCGGCTG GACAGAACAG GAAGAACAAC AGGCATTTGA AGCCGGCTTT CAGGCGGTTA CACTCGGAAA ACGGATTTTA CGCACAGAAA CCGCCCCACT CGCCGCCATC GCCGCGATGC AGACGCTTTG GGGCGATTTT ACCTAG
Details for NegoA.00176.a.B1.GE39960
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMP RFYLPENLSV GQTVDLPDNI VRHLNVLRVR PNENITLFDG KGKAHTARLT VLEKHRAEAE ILHEDTTDNE SPLNITLIQS ISSGDRMDFT LQKSVELGVT AIQPVISERC IVRLDGERAA KRLARWQEIV ISACEQSGRN TVPPVLPIIG YREALDKMPS ENTKLIMSIN RACKLGDIRH PSGAIVFMVG PEGGWTEQEE QQAFEAGFQA VTLGKRILRT ETAPLAAIAA MQTLWGDFT
Validated NT Sequence
atcttactca cttaggtaaa atcgccccaa agcgtctgca tcgcggcgat ggcggcgagt ggggcggttt ctgtgcgtaa aatccgtttt ccgagtgtaa ccgcctgaaa gccggcttca aatgcctgtt gttcttcctg ttctgtccag ccgccttcgg gcccgaccat aaagacgatt gcgccggacg ggtggcggat gtcgccgagt ttgcaggcgc ggttgatgct cataatcagc ttggtgtttt cagacggcat tttgtcgagt gcttcacggt agccgatgat gggcagtacg gggggaacgg tgttcctgcc gctttgttcg cacgcggaga tgacgatttc ctgccagcgt gcgaggcgtt tggcggcgcg ttctccgtcg aggcggacga tgcagcgttc gctgatgacg ggctgtatgg cggttacgcc gagttcgacg cttttttgca gggtgaaatc catgcgatcg ccggaggaaa tggattggat gagcgtgatg ttgagcgggg actcgttgtc ggttgtgtct tcgtgcagga tttcggcttc ggcgcggtgt ttttccaaaa cggtcagccg tgcggtatgt gccttgcctt tgccgtcgaa gagggtgatg ttttcgttgg ggcggacgcg caggacgttg aggtggcgga cgatgttgtc gggaaggtcg acggtttgtc cgacggaaag gttttcgggc aggtagaatc tgggcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMP RFYLPENLSV GQTVALPDNI VRHLNVLRVR PNENITLFDG KGKAHTARLT VLEKHRAEAE ILHEDTTDNE SPLNITLIQS ISSGDRMDFT LQKSVELGVT AIQPVISERC IVRLDGERAA KRLARWQEIV ISACEQSGRN TVPPVLPIIG YREALDKMPS ESTRLIMSIN RARKLGDIRH PSGAIVFMVG PEGGWTEQEE QQAFEAGFQA VTLGKRILRT ETAPLAAIAA MQTLWGDFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca gattctacct gcccgaaaac ctttccgtcg gacaaaccgt cgccctgccc gacaacatcg tccgccacct caacgtcctg cgcgtccgcc ccaacgaaaa catcaccctc ttcgacggca aaggcaaggc acataccgca cggctgaccg ttttggaaaa acaccgcgcc gaagccgaaa tcctgcacga agacacaacc gacaacgagt ccccgctcaa catcacgctc atccaatcca tttcctccgg cgatcgcatg gatttcaccc tgcaaaaaag cgtcgaactc ggcgtaaccg ccatacagcc cgtcatcagc gaacgctgca tcgtccgcct cgacggagaa cgcgccgcca aacgcctcgc acgctggcag gaaatcgtca tctccgcgtg cgaacaaagc ggcaggaaca ccgttccccc cgtactgccc atcatcggct accgtgaagc actcgacaaa atgccgtctg aaagcaccag gctgattatg agcatcaacc gcgcccgcaa actcggcgac atccgccacc cgtccggcgc aatcgtcttt atggtcgggc ccgaaggcgg ctggacagaa caggaagaac aacaggcatt tgaagccggc tttcaggcgg ttacactcgg aaaacggatt ttacgcacag aaaccgcccc actcgccgcc atcgccgcga tgcagacgct ttggggcgat tttacctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NegoA.00176.a.B1.PW37905
| PURIFICATION DATe: | 5/11/2016 |
| CONCENTRATION: | 20mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP RFYLPENLSV GQTVDLPDNI VRHLNVLRVR PNENITLFDG KGKAHTARLT VLEKHRAEAE ILHEDTTDNE SPLNITLIQS ISSGDRMDFT LQKSVELGVT AIQPVISERC IVRLDGERAA KRLARWQEIV ISACEQSGRN TVPPVLPIIG YREALDKMPS ENTKLIMSIN RACKLGDIRH PSGAIVFMVG PEGGWTEQEE QQAFEAGFQA VTLGKRILRT ETAPLAAIAA MQTLWGDFT
Validated NT Sequence
atcttactca cttaggtaaa atcgccccaa agcgtctgca tcgcggcgat ggcggcgagt ggggcggttt ctgtgcgtaa aatccgtttt ccgagtgtaa ccgcctgaaa gccggcttca aatgcctgtt gttcttcctg ttctgtccag ccgccttcgg gcccgaccat aaagacgatt gcgccggacg ggtggcggat gtcgccgagt ttgcaggcgc ggttgatgct cataatcagc ttggtgtttt cagacggcat tttgtcgagt gcttcacggt agccgatgat gggcagtacg gggggaacgg tgttcctgcc gctttgttcg cacgcggaga tgacgatttc ctgccagcgt gcgaggcgtt tggcggcgcg ttctccgtcg aggcggacga tgcagcgttc gctgatgacg ggctgtatgg cggttacgcc gagttcgacg cttttttgca gggtgaaatc catgcgatcg ccggaggaaa tggattggat gagcgtgatg ttgagcgggg actcgttgtc ggttgtgtct tcgtgcagga tttcggcttc ggcgcggtgt ttttccaaaa cggtcagccg tgcggtatgt gccttgcctt tgccgtcgaa gagggtgatg ttttcgttgg ggcggacgcg caggacgttg aggtggcgga cgatgttgtc gggaaggtcg acggtttgtc cgacggaaag gttttcgggc aggtagaatc tgggcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMP RFYLPENLSV GQTVALPDNI VRHLNVLRVR PNENITLFDG KGKAHTARLT VLEKHRAEAE ILHEDTTDNE SPLNITLIQS ISSGDRMDFT LQKSVELGVT AIQPVISERC IVRLDGERAA KRLARWQEIV ISACEQSGRN TVPPVLPIIG YREALDKMPS ESTRLIMSIN RARKLGDIRH PSGAIVFMVG PEGGWTEQEE QQAFEAGFQA VTLGKRILRT ETAPLAAIAA MQTLWGDFT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCA GATTCTACCT GCCCGAAAAC CTTTCCGTCG GACAAACCGT CGCCCTGCCC GACAACATCG TCCGCCACCT CAACGTCCTG CGCGTCCGCC CCAACGAAAA CATCACCCTC TTCGACGGCA AAGGCAAGGC ACATACCGCA CGGCTGACCG TTTTGGAAAA ACACCGCGCC GAAGCCGAAA TCCTGCACGA AGACACAACC GACAACGAGT CCCCGCTCAA CATCACGCTC ATCCAATCCA TTTCCTCCGG CGATCGCATG GATTTCACCC TGCAAAAAAG CGTCGAACTC GGCGTAACCG CCATACAGCC CGTCATCAGC GAACGCTGCA TCGTCCGCCT CGACGGAGAA CGCGCCGCCA AACGCCTCGC ACGCTGGCAG GAAATCGTCA TCTCCGCGTG CGAACAAAGC GGCAGGAACA CCGTTCCCCC CGTACTGCCC ATCATCGGCT ACCGTGAAGC ACTCGACAAA ATGCCGTCTG AAAGCACCAG GCTGATTATG AGCATCAACC GCGCCCGCAA ACTCGGCGAC ATCCGCCACC CGTCCGGCGC AATCGTCTTT ATGGTCGGGC CCGAAGGCGG CTGGACAGAA CAGGAAGAAC AACAGGCATT TGAAGCCGGC TTTCAGGCGG TTACACTCGG AAAACGGATT TTACGCACAG AAACCGCCCC ACTCGCCGCC ATCGCCGCGA TGCAGACGCT TTGGGGCGAT TTTACCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T