SSGCID: Target Page

NegoA.00169.a

replicative DNA helicase

CENTER ID:	NegoA.00169.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00169.a.B1.GE39959	full length		1	468

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00169.a.B1.PS37997	full length		1	468

Molecular models

METHOD	RESULTS
de-novo with evolutionary constraints	ModelArchive_ma-6t4bx

External Resources

RESOURCE	REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNDYAAMPPE GREVGALSLP PHSMEAEQSV LGGLMLENPA WDRIADVVSG EDFYRHEHRL IFRSIAKLIN EGRPADVITV QEDLQRNEEL EAAGGFEYLI TLAQNTPSAA NIRRYAEIVR ERSIMRQLAE VGTEIARSAY NPQGRDAGRL LDEAENKVFQ IAESTAKSKQ GFLEMPDLLK EVVQRIDMLY SRDNPDEVTG VPTGFIDLDK KTSGLQPGDL IIVAGRPSMG KTAFSINIAE YVAIEKHLPV AVFSMEMGGA QLVMRMLGSV GRLDQSVLKT GRLEDEHWGR LNEAVVKLSD APVYIDETPG LTALELRARA RRLARRFDGR LGLIVIDYLQ LMAASGRSDN RASELGEISR SLKALAKELQ VPIIALSQLS RTVEQRTDKR PMMSDLRESG AIEQDADLIM FMYRDEYYNQ DSPMKGLAEC IIGKHRNGPV GKIFLTWTGQ FTKFDNAAYV PEEAKIED

NT Sequence

ATGAACGATT ACGCAGCTAT GCCGCCTGAA GGCCGTGAGG TCGGCGCATT GTCGCTGCCT CCGCATTCGA TGGAGGCGGA ACAATCCGTT TTGGGCGGTT TGATGCTGGA AAATCCGGCT TGGGACAGGA TTGCCGATGT GGTTTCGGGA GAGGATTTCT ACCGCCACGA ACACCGCCTG ATTTTCCGAT CCATTGCCAA ATTAATCAAT GAGGGCCGTC CCGCAGATGT GATTACGGTT CAGGAAGATT TGCAGCGGAA CGAAGAATTG GAAGCGGCAG GCGGATTTGA ATATCTGATT ACACTGGCGC AAAACACCCC GTCTGCCGCA AACATCCGCC GCTACGCCGA AATCGTACGC GAGCGTTCCA TTATGCGCCA ACTCGCCGAA GTGGGGACGG AAATCGCCCG CAGCGCATAC AATCCGCAAG GCAGGGACGC GGGGCGGCTT TTGGATGAGG CGGAAAACAA AGTGTTTCAA ATCGCCGAAA GCACCGCCAA ATCCAAGCAG GGCTTTTTGG AGATGCCCGA TTTGCTGAAA GAAGTCGTAC AGCGCATCGA TATGCTTTAT TCGCGCGACA ATCCCGATGA AGTTACCGGC GTACCGACAG GGTTCATCGA CCTTGATAAA AAAACCTCGG GTCTGCAGCC CGGCGACCTG ATTATCGTCG CCGGCCGCCC GTCTATGGGT AAAACCGCGT TCTCCATCAA TATTGCCGAG TATGTCGCCA TTGAAAAACA TTTGCCCGTC GCCGTTTTCT CGATGGAAAT GGGCGGTGCG CAGTTGGTCA TGCGTATGCT CGGCTCGGTC GGACGGCTGG ATCAAAGCGT TTTGAAAACC GGCAGGCTCG AAGACGAACA CTGGGGCCGT CTGAACGAAG CAGTCGTCAA ACTCTCAGAC GCGCCCGTGT ACATCGACGA GACCCCGGGC CTGACTGCGC TCGAACTGCG CGCCCGCGCC CGCCGCCTCG CCCGCCGGTT TGACGGCAGA TTAGGCTTGA TCGTCATCGA CTACCTGCAA CTGATGGCGG CATCCGGCCG TTCGGACAAC CGCGCTTCAG AGCTTGGCGA AATTTCCCGT TCCCTCAAAG CCCTGGCAAA AGAGTTGCAA GTCCCCATCA TCGCCCTGTC GCAATTGAGC CGCACTGTCG AACAGCGTAC CGACAAACGC CCGATGATGT CCGACTTGAG GGAGTCGGGG GCAATCGAGC AGGATGCCGA CCTGATTATG TTTATGTACC GCGACGAATA CTACAACCAG GACTCACCCA TGAAAGGCCT TGCCGAATGT ATCATCGGCA AACACCGCAA CGGTCCCGTC GGTAAAATCT TCCTCACATG GACGGGACAA TTCACCAAAT TCGACAATGC TGCCTATGTT CCCGAGGAGG CAAAGATAGA AGATTAA

Details for NegoA.00169.a.B1.GE39959

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	53kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMN DYAAMPPEGR EVGALSLPPH SMEAEQSVLG GLMLENPAWD RIADVVSGED FYRHEHRLIF RSIAKLINEG RPADVITVQE DLQRNEELEA AGGFEYLITL AQNTPSAANI RRYAEIVRER SIMRQLAEVG TEIARSAYNP QGRDAGRLLD EAENKVFQIA ESTAKSKQGF LEMPDLLKEV VQRIDMLYSR DNPDEVTGVP TGFIDLDKKT SGLQPGDLII VAGRPSMGKT AFSINIAEYV AIEKHLPVAV FXMEMGGAQL VMRMLGSVGR LDQSVLKTXR LXDEHWGRLN EAVVKLSDAP VYIDETPGLT ALELRARARR LARQFNNKLG LIVIDYLQLM AASGRSDNRA SELGEISRSL KALAKELQVP IIALSQLSRT VEQRTDKRPM MSDLRESGAI EQDADLIMFM YRDEYYNQDS PMKGLAECII GKHRNGPVGK IFLTWTGQFT KFDNAA

Validated NT Sequence

aggcagcatt gtcgaatttg gtgaattgtc ccgtccatgt gaggaagatt ttaccgacgg gaccgttgcg gtgtttgccg atgatacatt cggcaaggcc tttcatgggt gagtcctggt tgtagtattc gtcgcggtac atgaacataa tcaggtcggc atcctgctcg atagcaccgg attcgcggag gtcggacatc atcgggcgtt tgtcggtgcg ctgttcgaca gtgcggctca attgcgacag ggcgatgatg gggacttgca actcttttgc cagggctttg agggaacggg aaatttcgcc aagctcggaa gcgcggttgt ccgaacggcc ggatgccgcc atcagttgca ggtagtcaat gacgattaat cccagcttat tgttaaattg acgggcgaga cgacgggcgc gggcgcgcag ttcgagcgca gtcaggcccg gggtctcgtc gatgtacacg ggcgcgtctg agagtttgac gactgcttcg ttcagacggc cccagtgttc gtcttngagc ctnnnggttt tcaaaacgct ttgatccagc cgtccgaccg agccgagcat acgcatgacc aactgcgcac cgcccatttc catcnagaaa acggcgacgg gcaaatgttt ttcaatggcg acatactcgg caatattgat ggagaacgcg gttttaccca tagacgggcg gccggcgacg ataatcaggt cgccgggctg cagacccgag gtttttttat caaggtcgat gaaccctgtc ggtacgccgg taacttcatc gggattgtcg cgcgaataaa gcatatcgat gcgctgtacg acttctttca gcaaatcggg catctccaaa aagccctgct tggatttggc ggtgctttcg gcgatttgaa acactttgtt ttccgcctca tccaaaagcc gccccgcgtc cctgccttgc ggattgtatg cgctgcgggc gatttccgtc cccacttcgg cgagttggcg cataatggaa cgctcgcgta cgatttcggc gtagcggcgg atgtttgcgg cagacggggt gttttgcgcc agtgtaatca gatattcaaa tccgcctgcc gcttccaatt cttcgttccg ctgcaaatct tcctgaaccg taatcacatc tgcgggacgg ccctcattga ttaatttggc aatggatcgg aaaatcaggc ggtgttcgtg gcggtagaaa tcctctcccg aaaccacatc ggcaatcctg tcccaagccg gattttccag catcaaaccg cccaaaacgg attgttccgc ctccatcgaa tgcggaggca gcgacaatgc gccgacctca cggccttcag gcggcatagc tgcgtaatcg ttcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMN DYAAMPPEGR EVGALSLPPH SMEAEQSVLG GLMLENPAWD RIADVVSGED FYRHEHRLIF RSIAKLINEG RPADVITVQE DLQRNEELEA AGGFEYLITL AQNTPSAANI RRYAEIVRER SIMRQLAEVG TEIARSAYNP QGRDAGRLLD EAENKVFQIA ESTAKSKQGF LEMPDLLKEV VQRIDMLYSR DNPDEVTGVP TGFIDLDKKT SGLQPGDLII VAGRPSMGKT AFSINIAEYV AIEKHLPVAV FSMEMGGAQL VMRMLGSVGR LDQSVLKTGR LEDEHWGRLN EAVVKLSDAP VYIDETPGLT ALELRARARR LARRFDGRLG LIVIDYLQLM AASGRSDNRA SELGEISRSL KALAKELQVP IIALSQLSRT VEQRTDKRPM MSDLRESGAI EQDADLIMFM YRDEYYNQDS PMKGLAECII GKHRNGPVGK IFLTWTGQFT KFDNAAYVPE EAKIED

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg attacgcagc tatgccgcct gaaggccgtg aggtcggcgc attgtcgctg cctccgcatt cgatggaggc ggaacaatcc gttttgggcg gtttgatgct ggaaaatccg gcttgggaca ggattgccga tgtggtttcg ggagaggatt tctaccgcca cgaacaccgc ctgattttcc gatccattgc caaattaatc aatgagggcc gtcccgcaga tgtgattacg gttcaggaag atttgcagcg gaacgaagaa ttggaagcgg caggcggatt tgaatatctg attacactgg cgcaaaacac cccgtctgcc gcaaacatcc gccgctacgc cgaaatcgta cgcgagcgtt ccattatgcg ccaactcgcc gaagtgggga cggaaatcgc ccgcagcgca tacaatccgc aaggcaggga cgcggggcgg cttttggatg aggcggaaaa caaagtgttt caaatcgccg aaagcaccgc caaatccaag cagggctttt tggagatgcc cgatttgctg aaagaagtcg tacagcgcat cgatatgctt tattcgcgcg acaatcccga tgaagttacc ggcgtaccga cagggttcat cgaccttgat aaaaaaacct cgggtctgca gcccggcgac ctgattatcg tcgccggccg cccgtctatg ggtaaaaccg cgttctccat caatattgcc gagtatgtcg ccattgaaaa acatttgccc gtcgccgttt tctcgatgga aatgggcggt gcgcagttgg tcatgcgtat gctcggctcg gtcggacggc tggatcaaag cgttttgaaa accggcaggc tcgaagacga acactggggc cgtctgaacg aagcagtcgt caaactctca gacgcgcccg tgtacatcga cgagaccccg ggcctgactg cgctcgaact gcgcgcccgc gcccgccgcc tcgcccgccg gtttgacggc agattaggct tgatcgtcat cgactacctg caactgatgg cggcatccgg ccgttcggac aaccgcgctt cagagcttgg cgaaatttcc cgttccctca aagccctggc aaaagagttg caagtcccca tcatcgccct gtcgcaattg agccgcactg tcgaacagcg taccgacaaa cgcccgatga tgtccgactt gagggagtcg ggggcaatcg agcaggatgc cgacctgatt atgtttatgt accgcgacga atactacaac caggactcac ccatgaaagg ccttgccgaa tgtatcatcg gcaaacaccg caacggtccc gtcggtaaaa tcttcctcac atggacggga caattcacca aattcgacaa tgctgcctat gttcccgagg aggcaaagat agaagattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for NegoA.00169.a.B1.PS37997

PURIFICATION DATe:	7/27/2016
CONCENTRATION:	11.87mg/ml
OBSERVED MW:	53kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMN DYAAMPPEGR EVGALSLPPH SMEAEQSVLG GLMLENPAWD RIADVVSGED FYRHEHRLIF RSIAKLINEG RPADVITVQE DLQRNEELEA AGGFEYLITL AQNTPSAANI RRYAEIVRER SIMRQLAEVG TEIARSAYNP QGRDAGRLLD EAENKVFQIA ESTAKSKQGF LEMPDLLKEV VQRIDMLYSR DNPDEVTGVP TGFIDLDKKT SGLQPGDLII VAGRPSMGKT AFSINIAEYV AIEKHLPVAV FSMEMGGAQL VMRMLGSVGR LDQSVLKTGR LEDEHWGRLN EAVVKLSDAP VYIDETPGLT ALELRARARR LARRFDGRLG LIVIDYLQLM AASGRSDNRA SELGEISRSL KALAKELQVP IIALSQLSRT VEQRTDKRPM MSDLRESGAI EQDADLIMFM YRDEYYNQDS PMKGLAECII GKHRNGPVGK IFLTWTGQFT KFDNAAYVPE EAKIED

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG ATTACGCAGC TATGCCGCCT GAAGGCCGTG AGGTCGGCGC ATTGTCGCTG CCTCCGCATT CGATGGAGGC GGAACAATCC GTTTTGGGCG GTTTGATGCT GGAAAATCCG GCTTGGGACA GGATTGCCGA TGTGGTTTCG GGAGAGGATT TCTACCGCCA CGAACACCGC CTGATTTTCC GATCCATTGC CAAATTAATC AATGAGGGCC GTCCCGCAGA TGTGATTACG GTTCAGGAAG ATTTGCAGCG GAACGAAGAA TTGGAAGCGG CAGGCGGATT TGAATATCTG ATTACACTGG CGCAAAACAC CCCGTCTGCC GCAAACATCC GCCGCTACGC CGAAATCGTA CGCGAGCGTT CCATTATGCG CCAACTCGCC GAAGTGGGGA CGGAAATCGC CCGCAGCGCA TACAATCCGC AAGGCAGGGA CGCGGGGCGG CTTTTGGATG AGGCGGAAAA CAAAGTGTTT CAAATCGCCG AAAGCACCGC CAAATCCAAG CAGGGCTTTT TGGAGATGCC CGATTTGCTG AAAGAAGTCG TACAGCGCAT CGATATGCTT TATTCGCGCG ACAATCCCGA TGAAGTTACC GGCGTACCGA CAGGGTTCAT CGACCTTGAT AAAAAAACCT CGGGTCTGCA GCCCGGCGAC CTGATTATCG TCGCCGGCCG CCCGTCTATG GGTAAAACCG CGTTCTCCAT CAATATTGCC GAGTATGTCG CCATTGAAAA ACATTTGCCC GTCGCCGTTT TCTCGATGGA AATGGGCGGT GCGCAGTTGG TCATGCGTAT GCTCGGCTCG GTCGGACGGC TGGATCAAAG CGTTTTGAAA ACCGGCAGGC TCGAAGACGA ACACTGGGGC CGTCTGAACG AAGCAGTCGT CAAACTCTCA GACGCGCCCG TGTACATCGA CGAGACCCCG GGCCTGACTG CGCTCGAACT GCGCGCCCGC GCCCGCCGCC TCGCCCGCCG GTTTGACGGC AGATTAGGCT TGATCGTCAT CGACTACCTG CAACTGATGG CGGCATCCGG CCGTTCGGAC AACCGCGCTT CAGAGCTTGG CGAAATTTCC CGTTCCCTCA AAGCCCTGGC AAAAGAGTTG CAAGTCCCCA TCATCGCCCT GTCGCAATTG AGCCGCACTG TCGAACAGCG TACCGACAAA CGCCCGATGA TGTCCGACTT GAGGGAGTCG GGGGCAATCG AGCAGGATGC CGACCTGATT ATGTTTATGT ACCGCGACGA ATACTACAAC CAGGACTCAC CCATGAAAGG CCTTGCCGAA TGTATCATCG GCAAACACCG CAACGGTCCC GTCGGTAAAA TCTTCCTCAC ATGGACGGGA CAATTCACCA AATTCGACAA TGCTGCCTAT GTTCCCGAGG AGGCAAAGAT AGAAGATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT