NegoA.00162.a

DNA gyrase subunit B

CENTER ID: NegoA.00162.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.00162.a.B1.GE39955 full length 1 796
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.2714
UniProt: B4RR46

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEQKHEEYG ADSIQVLEGL EAVRKRPGMY IGDTQDGSGL HHMVFEVLDN AIDEALAGHC DKITVTIHAD HSVSVADNGR GMPTGIHPKE GRSAAEVIMT VLHAGGKFDN NSYKISGGLH GVGVSVVNAL SDWVTLTIYR DGKEHFVRFV RGETEEPLKI VGDSDKKGTT VRFLAGTETF GNIEYSFDIL AKRIRELSFL NNGVDIELTD ERDGKHESFA LSGGVAGFVQ YMNRKKTPLH EKIFYAFGEK DGMSVECAMQ WNDSYQESVQ CFTNNIPQRD GGTHLTALRQ VMTRTINSYI EANEVAKKAK VETAGDDMRE GLTCVLSVKL PDPKFSSQTK DKLVSGEIGP VVNEVINQAL TDFLEENPNE AKIITGKIVD AARAREAARK AREITRRKGV MDGLGLPGKL ADCQEKDPAL SELYLVEGDS AGGSAMQGRD RKFQAILPLK GKILNVEKAR FEKMLASQEV ATLITALGAG IGKEEFNPEK LRYHRIIIMT DADVDGAHIR TLLLTFFYRQ MPELVERGYI YIAQPPLYKA KYGKQERYLK DELEKDQWLL GLALEKAKIV SDGRTIEGAE LADTAKQFLL AKTVIEQESR FVDELVLRAM LHASPIDLTS SENADKAVAE LSGLLDEKEA ALERIEGHEG HRFIKITRKL HGNVMVSYIE PKFLNSKAYQ TLTQTAAALK GLVGEGAKLY KGENEYDADS FETALDILMS VAQKGMSIQR YKGLGEMNPE QLWETTMDPA VRRLLKVRIE DAIAADEVFV TLMGDEVEPR RAFIENNALI AQNIDA
NT Sequence
ATGACTGAAC AAAAACACGA AGAATACGGC GCCGACAGCA TCCAGGTGCT CGAAGGCTTG GAAGCGGTAC GCAAACGCCC CGGCATGTAC ATCGGCGACA CGCAGGACGG CAGCGGGCTG CACCATATGG TGTTTGAAGT ATTGGACAAC GCCATCGACG AAGCACTCGC CGGACATTGC GACAAAATCA CGGTAACGAT ACACGCCGAC CATTCCGTCA GCGTCGCCGA CAACGGGCGC GGTATGCCCA CCGGCATCCA CCCGAAAGAA GGGCGTTCCG CCGCCGAAGT CATCATGACC GTCTTGCACG CGGGCGGCAA ATTCGACAAC AACAGCTACA AAATCTCCGG CGGCCTGCAC GGCGTGGGCG TATCCGTCGT CAACGCGCTG TCCGACTGGG TAACGCTGAC CATCTACCGC GACGGCAAAG AACACTTCGT CCGCTTCGTA CGCGGCGAAA CCGAAGAGCC GCTGAAAATT GTCGGCGATT CCGACAAAAA AGGCACGACC GTGCGCTTCC TCGCCGGCAC GGAAACCTTC GGCAATATCG AATACAGCTT CGACATCCTC GCCAAACGTA TTCGCGAACT TTCGTTCCTA AACAACGGCG TGGACATCGA ATTGACCGAC GAGCGCGACG GCAAGCACGA AAGCTTCGCC CTTTCCGGCG GCGTGGCGGG CTTCGTGCAA TACATGAACC GCAAAAAAAC GCCCTTGCAC GAAAAAATCT TCTATGCGTT CGGCGAGAAA GACGGCATGA GCGTCGAATG CGCAATGCAA TGGAACGACA GCTATCAGGA AAGCGTGCAG TGCTTCACCA ACAACATCCC TCAGCGCGAC GGCGGTACGC ACCTGACCGC GCTGCGCCAA GTGATGACGC GCACCATCAA CAGCTACATC GAAGCTAACG AAGTCGCCAA AAAAGCCAAA GTGGAAACCG CCGGCGACGA TATGCGCGAA GGTTTGACCT GCGTGTTGTC CGTCAAACTG CCCGACCCCA AATTCTCATC CCAAACCAAA GACAAACTGG TTTCCGGCGA AATCGGCCCC GTTGTCAACG AAGTCATCAA CCAAGCACTA ACCGACTTCC TCGAAGAAAA TCCGAACGAA GCCAAAATCA TCACCGGCAA AATCGTCGAT GCCGCCCGCG CACGCGAAGC CGCCCGCAAA GCCCGCGAAA TCACCCGCCG CAAAGGCGTG ATGGACGGCT TGGGACTGCC CGGCAAACTC GCCGACTGCC AAGAAAAAGA CCCTGCCCTG TCTGAACTCT ACCTCGTCGA GGGCGACTCC GCAGGCGGTT CCGCCATGCA GGGCCGCGAC CGCAAATTCC AAGCGATTTT GCCGCTCAAA GGTAAAATTT TGAACGTCGA AAAAGCACGT TTTGAAAAAA TGCTCGCCAG CCAAGAGGTC GCCACCCTGA TTACCGCGCT GGGTGCAGGC ATCGGCAAAG AAGAGTTCAA CCCTGAAAAA CTACGCTACC ACCGCATCAT CATCATGACC GATGCCGACG TGGACGGTGC GCACATCCGC ACCCTGCTCC TGACCTTCTT CTACCGCCAA ATGCCCGAAC TGGTCGAGCG CGGCTACATT TACATCGCCC AGCCGCCGCT CTACAAAGCC AAATACGGCA AGCAGGAGCG TTACCTCAAA GACGAACTGG AAAAAGACCA ATGGCTGCTC GGCCTTGCCT TGGAAAAAGC CAAAATCGTT TCAGACGGCC GCACCATCGA AGGCGCAGAA CTTGCCGACA CCGCCAAACA ATTCTTGTTG GCGAAAACCG TCATCGAACA GGAAAGCCGC TTCGTGGACG AACTCGTCCT GCGTGCCATG CTGCACGCGT CGCCCATTGA TTTGACGTCG TCTGAAAACG CCGATAAAGC CGTTGCCGAA CTTTCCGGTT TGCTTGACGA AAAAGAAGCC GCCCTCGAAC GCATCGAAGG TCATGAAGGA CACCGGTTCA TCAAAATCAC GCGCAAGCTG CACGGCAACG TCATGGTCAG CTACATCGAA CCCAAGTTCC TCAACAGCAA AGCCTACCAA ACCCTCACCC AAACCGCCGC CGCGCTCAAA GGCTTGGTCG GCGAGGGCGC CAAGCTCTAC AAAGGCGAGA ACGAGTACGA CGCGGACAGC TTTGAAACCG CTTTGGACAT CTTGATGAGC GTTGCCCAAA AAGGTATGTC CATCCAACGA TACAAAGGTT TGGGCGAGAT GAACCCCGAG CAGCTTTGGG AAACCACGAT GGATCCCGCC GTGCGCCGCC TGTTGAAAGT GCGCATCGAA GATGCCATTG CCGCCGACGA AGTGTTCGTT ACCCTGATGG GCGACGAGGT CGAACCGCGC CGCGCCTTTA TCGAAAACAA TGCGCTGATT GCGCAAAATA TCGACGCATA A
Details for NegoA.00162.a.B1.GE39955
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 89kDa
OBSERVED MW: 89kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT EQKHEEYGAD SIQVLEGLEA VRKRPGMYIG DTQDGSGLHH MVFEVLDNAI DEALAGHCDK ITVTIHADHS VSVADNGRGM PTGIHPKEGR SAAEVIMTVL HAGGKFDNNS YKISGGLHGV GVSVVNALSD WVTLTIYRDG KEHFVRFVRG ETEEPLKIVG DSDKKGTTVR FLAGTETFGN IEYSFDILAK RIRELSFLNN GVDIELTDER DGKHESFALS GGVAGFVQYM NRKKTPLHEK IFYAFGEKDG MSVECAMQWN DSYQESVQCF TNNIPQRDGG THLTALRQVM TRTINSYIEA NEVAKKAKVE TAGDDMREGL TCVLSVKLPD PKFSSQTKDK LVSGEIGPVV NEVINQALTD FLEENPNEAK IITGKIVDAA RAREAARKAR EITRRKGVMD GLGLPGKLAD CQEKDPALSE LYLVEGDSAG GSAMQGRDRK FQAILPLKGK ILNVEKARFE KMLASQEVAT LITALGAGIG KEEFNPEKLR YHRIIIMTDA DVDGAHIRTL LLTFFYRQMP ELVERGYIYI AQPPLYKAKY GKQERYLKDE LEKDQWLLGL ALEKAKIVSD GRTIEGAELA DTAKQFLLAK TVIEQESRFV DELVLRAMLH ASPIDLTSSE NADKAVAELS GLLDEKEAAL ERIEGHEGHR FIKITRKLHG NVMVSYIEPK FLNSKAYQTL TQTAAALKGL VGEGAKLYKG ENEYDADSFE TALDILMSVA QKGMSIQRYK GLGEMNPEQL WETTMDPAVR RLLKVRIEDA IAADEVFVTL MGDEVEPRRA FIENNALIAQ NIDA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg aacaaaaaca cgaagaatac ggcgccgaca gcatccaggt gctcgaaggc ttggaagcgg tacgcaaacg ccccggcatg tacatcggcg acacgcagga cggcagcggg ctgcaccata tggtgtttga agtattggac aacgccatcg acgaagcact cgccggacat tgcgacaaaa tcacggtaac gatacacgcc gaccattccg tcagcgtcgc cgacaacggg cgcggtatgc ccaccggcat ccacccgaaa gaagggcgtt ccgccgccga agtcatcatg accgtcttgc acgcgggcgg caaattcgac aacaacagct acaaaatctc cggcggcctg cacggcgtgg gcgtatccgt cgtcaacgcg ctgtccgact gggtaacgct gaccatctac cgcgacggca aagaacactt cgtccgcttc gtacgcggcg aaaccgaaga gccgctgaaa attgtcggcg attccgacaa aaaaggcacg accgtgcgct tcctcgccgg cacggaaacc ttcggcaata tcgaatacag cttcgacatc ctcgccaaac gtattcgcga actttcgttc ctaaacaacg gcgtggacat cgaattgacc gacgagcgcg acggcaagca cgaaagcttc gccctttccg gcggcgtggc gggcttcgtg caatacatga accgcaaaaa aacgcccttg cacgaaaaaa tcttctatgc gttcggcgag aaagacggca tgagcgtcga atgcgcaatg caatggaacg acagctatca ggaaagcgtg cagtgcttca ccaacaacat ccctcagcgc gacggcggta cgcacctgac cgcgctgcgc caagtgatga cgcgcaccat caacagctac atcgaagcta acgaagtcgc caaaaaagcc aaagtggaaa ccgccggcga cgatatgcgc gaaggtttga cctgcgtgtt gtccgtcaaa ctgcccgacc ccaaattctc atcccaaacc aaagacaaac tggtttccgg cgaaatcggc cccgttgtca acgaagtcat caaccaagca ctaaccgact tcctcgaaga aaatccgaac gaagccaaaa tcatcaccgg caaaatcgtc gatgccgccc gcgcacgcga agccgcccgc aaagcccgcg aaatcacccg ccgcaaaggc gtgatggacg gcttgggact gcccggcaaa ctcgccgact gccaagaaaa agaccctgcc ctgtctgaac tctacctcgt cgagggcgac tccgcaggcg gttccgccat gcagggccgc gaccgcaaat tccaagcgat tttgccgctc aaaggtaaaa ttttgaacgt cgaaaaagca cgttttgaaa aaatgctcgc cagccaagag gtcgccaccc tgattaccgc gctgggtgca ggcatcggca aagaagagtt caaccctgaa aaactacgct accaccgcat catcatcatg accgatgccg acgtggacgg tgcgcacatc cgcaccctgc tcctgacctt cttctaccgc caaatgcccg aactggtcga gcgcggctac atttacatcg cccagccgcc gctctacaaa gccaaatacg gcaagcagga gcgttacctc aaagacgaac tggaaaaaga ccaatggctg ctcggccttg ccttggaaaa agccaaaatc gtttcagacg gccgcaccat cgaaggcgca gaacttgccg acaccgccaa acaattcttg ttggcgaaaa ccgtcatcga acaggaaagc cgcttcgtgg acgaactcgt cctgcgtgcc atgctgcacg cgtcgcccat tgatttgacg tcgtctgaaa acgccgataa agccgttgcc gaactttccg gtttgcttga cgaaaaagaa gccgccctcg aacgcatcga aggtcatgaa ggacaccggt tcatcaaaat cacgcgcaag ctgcacggca acgtcatggt cagctacatc gaacccaagt tcctcaacag caaagcctac caaaccctca cccaaaccgc cgccgcgctc aaaggcttgg tcggcgaggg cgccaagctc tacaaaggcg agaacgagta cgacgcggac agctttgaaa ccgctttgga catcttgatg agcgttgccc aaaaaggtat gtccatccaa cgatacaaag gtttgggcga gatgaacccc gagcagcttt gggaaaccac gatggatccc gccgtgcgcc gcctgttgaa agtgcgcatc gaagatgcca ttgccgccga cgaagtgttc gttaccctga tgggcgacga ggtcgaaccg cgccgcgcct ttatcgaaaa caatgcgctg attgcgcaaa atatcgacgc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat