NegoA.00156.a

threonyl-tRNA synthetase

CENTER ID: NegoA.00156.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.00156.a.B1.GE39954 full length 1 637
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.00156.a.B1.PS37932 full length 1 637
Molecular models
METHOD RESULTS
comparative modelling Robetta_85031
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|521006.8.peg.489
UniProt: B4RJY3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNITLPDCS VRQYESPVTV AQIAASIGAG LAKAAVAGKV NGKLVDACDP IVEDSAVQII TPKDQEGIEI IRHSCAHLVG HAVKQLYPNA KMVIGPVIEE GFYYDIATEK PFTPEDVAAI EARMKELIAQ DYDVVKIMTP RAEAIKIFQE RGEEYKLRLI EDMPEVEAMG MYHHQEYVDM CRGPHVPNTR FLKNFKLTKL AGAYWRGDSN NEMLQRIYGT AWATKDELKD YIQRIEEAEK RDHRKLGKQL DLFHLQDEAP GMVFWHPKGW ALWQTIEQHM RKELNAAGYK EVKTPQIMDK TFWEKSGHWD NYKDNMFVTS SEKREYAVKP MNCPGHVQIF NNGLRSYRDL PMRLAEFGSC HRNEPSGALH GLMRVRGFVQ DDAHIFCTED QIVSEARAFN ELLVRIYKQF GFHDVSVRLS LRPEKRAGSD DVWDKAEQGL REALTACGVE WGELPGEGAF YGPKIEYHVK DALGRSWQCG TLQLDFVLPE RLDAEYVTEN NDRARPVMLH RAILGSLERF VGILIENHAG SFPLWLAPVQ MVIMNITENQ ADYCREVAAK LQAAGFRAEL DLRNEKIGYK IRDNSQYRFP YQIVIGDKEK QENKVAVRRK AEDLGSLDLD DFIAQLQQEI TDALVNH
NT Sequence
ATGTTGAATA TTACCTTGCC GGACTGTTCA GTCCGCCAAT ACGAATCCCC CGTTACCGTG GCTCAAATTG CTGCGTCTAT CGGTGCCGGT TTGGCGAAGG CGGCGGTGGC AGGCAAGGTA AACGGCAAAT TGGTCGATGC GTGCGACCCG ATTGTTGAGG ATTCTGCTGT TCAAATCATT ACTCCGAAAG ATCAGGAAGG CATCGAAATC ATCCGCCATT CCTGCGCACA TCTTGTCGGG CATGCCGTCA AGCAACTCTA TCCTAATGCA AAAATGGTTA TCGGCCCCGT CATTGAAGAG GGCTTTTATT ACGACATCGC CACGGAAAAA CCGTTTACAC CGGAAGATGT TGCCGCCATT GAAGCGCGTA TGAAAGAATT GATTGCCCAA GATTATGATG TGGTCAAAAT CATGACTCCG CGTGCGGAGG CGATTAAAAT TTTTCAAGAG CGCGGCGAAG AATACAAACT GCGCCTGATT GAAGATATGC CCGAAGTTGA AGCAATGGGT ATGTATCATC ATCAGGAATA TGTCGATATG TGCCGCGGTC CGCACGTTCC GAACACCCGC TTCCTGAAAA ACTTCAAGCT GACCAAGCTG GCAGGCGCAT ATTGGCGCGG CGACAGCAAT AATGAAATGC TGCAACGTAT TTATGGCACT GCTTGGGCGA CGAAAGACGA ATTAAAAGAC TATATCCAAC GCATTGAAGA AGCAGAAAAA CGTGACCACC GCAAATTGGG CAAGCAACTG GATTTGTTCC ACTTGCAGGA CGAAGCGCCG GGTATGGTGT TTTGGCATCC CAAAGGTTGG GCTTTGTGGC AGACTATCGA GCAGCATATG CGTAAAGAGC TGAACGCCGC CGGTTATAAA GAGGTCAAAA CGCCTCAAAT TATGGATAAA ACCTTTTGGG AAAAATCCGG CCACTGGGAC AACTACAAAG ACAATATGTT CGTAACCAGT TCGGAAAAAC GCGAATATGC GGTTAAACCG ATGAACTGTC CGGGTCATGT TCAAATTTTC AACAACGGTT TGCGTTCATA TCGAGATTTG CCGATGCGTT TGGCGGAATT CGGTTCTTGC CACCGCAACG AGCCGAGCGG CGCACTGCAC GGTCTGATGA GGGTTCGCGG TTTTGTGCAG GATGATGCGC ATATTTTTTG TACCGAAGAT CAAATCGTCA GCGAGGCTCG TGCGTTCAAT GAATTGTTGG TTCGCATCTA CAAACAGTTC GGTTTCCATG ATGTATCCGT CAGGCTTTCC CTTCGCCCTG AAAAACGCGC AGGTTCAGAC GACGTGTGGG ATAAGGCAGA GCAGGGTTTG CGCGAGGCAT TGACTGCCTG CGGCGTGGAA TGGGGCGAAT TGCCGGGCGA GGGTGCGTTT TACGGGCCTA AAATCGAATA TCACGTCAAA GATGCCTTGG GTCGTTCTTG GCAATGCGGT ACATTACAAC TGGATTTTGT CTTGCCGGAA CGCTTGGATG CAGAATATGT AACAGAAAAC AACGACCGAG CGCGGCCTGT TATGTTGCAT CGCGCCATTT TAGGTTCTTT GGAGCGGTTT GTCGGCATTC TGATTGAGAA CCATGCAGGC TCTTTCCCGT TATGGCTTGC ACCGGTTCAA ATGGTGATTA TGAACATCAC CGAAAATCAG GCAGATTATT GTCGGGAAGT TGCTGCCAAA TTGCAGGCGG CAGGCTTCCG CGCCGAGTTG GATTTGCGTA ATGAAAAAAT CGGTTACAAA ATCCGCGACA ACAGCCAATA CCGTTTCCCT TATCAAATCG TTATCGGCGA TAAAGAGAAA CAAGAAAACA AAGTTGCGGT ACGCCGCAAA GCGGAAGACT TGGGTTCTTT GGATTTGGAT GATTTCATTG CGCAATTGCA GCAAGAAATC ACTGATGCCC TCGTCAATCA TTAA
Details for NegoA.00156.a.B1.GE39954
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 74kDa
OBSERVED MW: 74kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML NITLPDCSVR QYESPVTVAQ IAASIGAGLA KAAVAGKVNG KLVDACDPIV EDSAVQIITP KDQEGIEIIR HSCAHLVGHA VKQLYPNAKM VIGPVIEEGF YYDIATEKPF TPEDVAAIEA RMKELIAQDY DVVKIMTPRA EAIKIFQERG EEYKLRLIED MPEVEAMGMY HHQEYVDMCR GPHVPNTRFL KNFKLTKLAG AYWRGDSNNE MLQRIYGTAW ATKDELKDYI QRIEEAEKRD HRKLGKQLDL FHLQDEAPGM VFWHPKGWAL WQTIEQHMRK ELNAAGYKEV KTPQIMDKTF WEKSGHWDNY KDNMFVTSSE KREYAVKPMN CPGHVQIFNN GLRSYRDLPM RLAEFGSCHR NEPSGALHGL MRVRGFVQDD AHIFCTEDQI VSEARAFNEL LVRIYKQFGF HDVSVRLSLR PEKRAGSDDV WDKAEQGLRE ALTACGVEWG ELPGEGAFYG PKIEYHVKDA LGRSWQCGTL QLDFVLPERL DAEYVTENND RARPVMLHRA ILGSLERFVG ILIENHAGSF PLWLAPVQMV IMNITENQAD YCREVAAKLQ AAGFRAELDL RNEKIGYKIR DNSQYRFPYQ IVIGDKEKQE NKVAVRRKAE DLGSLDLDDF IAQLQQEITD ALVNH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga atattacctt gccggactgt tcagtccgcc aatacgaatc ccccgttacc gtggctcaaa ttgctgcgtc tatcggtgcc ggtttggcga aggcggcggt ggcaggcaag gtaaacggca aattggtcga tgcgtgcgac ccgattgttg aggattctgc tgttcaaatc attactccga aagatcagga aggcatcgaa atcatccgcc attcctgcgc acatcttgtc gggcatgccg tcaagcaact ctatcctaat gcaaaaatgg ttatcggccc cgtcattgaa gagggctttt attacgacat cgccacggaa aaaccgttta caccggaaga tgttgccgcc attgaagcgc gtatgaaaga attgattgcc caagattatg atgtggtcaa aatcatgact ccgcgtgcgg aggcgattaa aatttttcaa gagcgcggcg aagaatacaa actgcgcctg attgaagata tgcccgaagt tgaagcaatg ggtatgtatc atcatcagga atatgtcgat atgtgccgcg gtccgcacgt tccgaacacc cgcttcctga aaaacttcaa gctgaccaag ctggcaggcg catattggcg cggcgacagc aataatgaaa tgctgcaacg tatttatggc actgcttggg cgacgaaaga cgaattaaaa gactatatcc aacgcattga agaagcagaa aaacgtgacc accgcaaatt gggcaagcaa ctggatttgt tccacttgca ggacgaagcg ccgggtatgg tgttttggca tcccaaaggt tgggctttgt ggcagactat cgagcagcat atgcgtaaag agctgaacgc cgccggttat aaagaggtca aaacgcctca aattatggat aaaacctttt gggaaaaatc cggccactgg gacaactaca aagacaatat gttcgtaacc agttcggaaa aacgcgaata tgcggttaaa ccgatgaact gtccgggtca tgttcaaatt ttcaacaacg gtttgcgttc atatcgagat ttgccgatgc gtttggcgga attcggttct tgccaccgca acgagccgag cggcgcactg cacggtctga tgagggttcg cggttttgtg caggatgatg cgcatatttt ttgtaccgaa gatcaaatcg tcagcgaggc tcgtgcgttc aatgaattgt tggttcgcat ctacaaacag ttcggtttcc atgatgtatc cgtcaggctt tcccttcgcc ctgaaaaacg cgcaggttca gacgacgtgt gggataaggc agagcagggt ttgcgcgagg cattgactgc ctgcggcgtg gaatggggcg aattgccggg cgagggtgcg ttttacgggc ctaaaatcga atatcacgtc aaagatgcct tgggtcgttc ttggcaatgc ggtacattac aactggattt tgtcttgccg gaacgcttgg atgcagaata tgtaacagaa aacaacgacc gagcgcggcc tgttatgttg catcgcgcca ttttaggttc tttggagcgg tttgtcggca ttctgattga gaaccatgca ggctctttcc cgttatggct tgcaccggtt caaatggtga ttatgaacat caccgaaaat caggcagatt attgtcggga agttgctgcc aaattgcagg cggcaggctt ccgcgccgag ttggatttgc gtaatgaaaa aatcggttac aaaatccgcg acaacagcca ataccgtttc ccttatcaaa tcgttatcgg cgataaagag aaacaagaaa acaaagttgc ggtacgccgc aaagcggaag acttgggttc tttggatttg gatgatttca ttgcgcaatt gcagcaagaa atcactgatg ccctcgtcaa tcattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NegoA.00156.a.B1.PS37932
PURIFICATION DATe: 6/1/2016
CONCENTRATION: 23mg/ml
OBSERVED MW: 74kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHML NITLPDCSVR QYESPVTVAQ IAASIGAGLA KAAVAGKVNG KLVDACDPIV EDSAVQIITP KDQEGIEIIR HSCAHLVGHA VKQLYPNAKM VIGPVIEEGF YYDIATEKPF TPEDVAAIEA RMKELIAQDY DVVKIMTPRA EAIKIFQERG EEYKLRLIED MPEVEAMGMY HHQEYVDMCR GPHVPNTRFL KNFKLTKLAG AYWRGDSNNE MLQRIYGTAW ATKDELKDYI QRIEEAEKRD HRKLGKQLDL FHLQDEAPGM VFWHPKGWAL WQTIEQHMRK ELNAAGYKEV KTPQIMDKTF WEKSGHWDNY KDNMFVTSSE KREYAVKPMN CPGHVQIFNN GLRSYRDLPM RLAEFGSCHR NEPSGALHGL MRVRGFVQDD AHIFCTEDQI VSEARAFNEL LVRIYKQFGF HDVSVRLSLR PEKRAGSDDV WDKAEQGLRE ALTACGVEWG ELPGEGAFYG PKIEYHVKDA LGRSWQCGTL QLDFVLPERL DAEYVTENND RARPVMLHRA ILGSLERFVG ILIENHAGSF PLWLAPVQMV IMNITENQAD YCREVAAKLQ AAGFRAELDL RNEKIGYKIR DNSQYRFPYQ IVIGDKEKQE NKVAVRRKAE DLGSLDLDDF IAQLQQEITD ALVNH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGA ATATTACCTT GCCGGACTGT TCAGTCCGCC AATACGAATC CCCCGTTACC GTGGCTCAAA TTGCTGCGTC TATCGGTGCC GGTTTGGCGA AGGCGGCGGT GGCAGGCAAG GTAAACGGCA AATTGGTCGA TGCGTGCGAC CCGATTGTTG AGGATTCTGC TGTTCAAATC ATTACTCCGA AAGATCAGGA AGGCATCGAA ATCATCCGCC ATTCCTGCGC ACATCTTGTC GGGCATGCCG TCAAGCAACT CTATCCTAAT GCAAAAATGG TTATCGGCCC CGTCATTGAA GAGGGCTTTT ATTACGACAT CGCCACGGAA AAACCGTTTA CACCGGAAGA TGTTGCCGCC ATTGAAGCGC GTATGAAAGA ATTGATTGCC CAAGATTATG ATGTGGTCAA AATCATGACT CCGCGTGCGG AGGCGATTAA AATTTTTCAA GAGCGCGGCG AAGAATACAA ACTGCGCCTG ATTGAAGATA TGCCCGAAGT TGAAGCAATG GGTATGTATC ATCATCAGGA ATATGTCGAT ATGTGCCGCG GTCCGCACGT TCCGAACACC CGCTTCCTGA AAAACTTCAA GCTGACCAAG CTGGCAGGCG CATATTGGCG CGGCGACAGC AATAATGAAA TGCTGCAACG TATTTATGGC ACTGCTTGGG CGACGAAAGA CGAATTAAAA GACTATATCC AACGCATTGA AGAAGCAGAA AAACGTGACC ACCGCAAATT GGGCAAGCAA CTGGATTTGT TCCACTTGCA GGACGAAGCG CCGGGTATGG TGTTTTGGCA TCCCAAAGGT TGGGCTTTGT GGCAGACTAT CGAGCAGCAT ATGCGTAAAG AGCTGAACGC CGCCGGTTAT AAAGAGGTCA AAACGCCTCA AATTATGGAT AAAACCTTTT GGGAAAAATC CGGCCACTGG GACAACTACA AAGACAATAT GTTCGTAACC AGTTCGGAAA AACGCGAATA TGCGGTTAAA CCGATGAACT GTCCGGGTCA TGTTCAAATT TTCAACAACG GTTTGCGTTC ATATCGAGAT TTGCCGATGC GTTTGGCGGA ATTCGGTTCT TGCCACCGCA ACGAGCCGAG CGGCGCACTG CACGGTCTGA TGAGGGTTCG CGGTTTTGTG CAGGATGATG CGCATATTTT TTGTACCGAA GATCAAATCG TCAGCGAGGC TCGTGCGTTC AATGAATTGT TGGTTCGCAT CTACAAACAG TTCGGTTTCC ATGATGTATC CGTCAGGCTT TCCCTTCGCC CTGAAAAACG CGCAGGTTCA GACGACGTGT GGGATAAGGC AGAGCAGGGT TTGCGCGAGG CATTGACTGC CTGCGGCGTG GAATGGGGCG AATTGCCGGG CGAGGGTGCG TTTTACGGGC CTAAAATCGA ATATCACGTC AAAGATGCCT TGGGTCGTTC TTGGCAATGC GGTACATTAC AACTGGATTT TGTCTTGCCG GAACGCTTGG ATGCAGAATA TGTAACAGAA AACAACGACC GAGCGCGGCC TGTTATGTTG CATCGCGCCA TTTTAGGTTC TTTGGAGCGG TTTGTCGGCA TTCTGATTGA GAACCATGCA GGCTCTTTCC CGTTATGGCT TGCACCGGTT CAAATGGTGA TTATGAACAT CACCGAAAAT CAGGCAGATT ATTGTCGGGA AGTTGCTGCC AAATTGCAGG CGGCAGGCTT CCGCGCCGAG TTGGATTTGC GTAATGAAAA AATCGGTTAC AAAATCCGCG ACAACAGCCA ATACCGTTTC CCTTATCAAA TCGTTATCGG CGATAAAGAG AAACAAGAAA ACAAAGTTGC GGTACGCCGC AAAGCGGAAG ACTTGGGTTC TTTGGATTTG GATGATTTCA TTGCGCAATT GCAGCAAGAA ATCACTGATG CCCTCGTCAA TCATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT