NegoA.00153.a

DNA primase

CENTER ID: NegoA.00153.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.00153.a.B1.GE39953 full length 1 590
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.856
UniProt: B4RKY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIPSDFIDEL LAKTDIVGII DEQVPLKKGG ANYMACCPFH KEKTPSFSVS PTKQFYHCFS CGAHGSAIGF VMEHQGLSFP EAVQFLADRV GMIVPKVRGQ NDNPEVRAER KKKQQTLEET TAAAADFYAQ QLKFNPAAKA YLDKRGLSAE VIAHYGLGYA PDGWQPLAQV FQPYPNTALV DTGMVIDNEG RHYDRFRHRI MFPIRNPRGQ VIGFGGRVLD DSKPKYLNSP DTPLFDKGKN LYGLYEGRAA VKEAERILVV EGYMDVVALA QFGVGYGVAA LGTATTAEHV KILMRQADSI YFCFDGDSAG RKAAWRALEN ALPQLKDDKS LHFLFLPEEH DPDSYIRAYG KAQFEDALLN QSKPLSEYFW EHLSDGIHLN TQEGKAELVK TSSPLLVQIT APALAYLLKQ RLSELVGIDP DNLAQLLGQE APKRHVKQKN YKLPPISVKQ PVMPTLVQRQ IRSLLINPDW AAYIDLPDYL ALDGDFACLA NLAETIKNHP SVPATAQVLE HMRGSPYEET IDRIFRSALQ SEEMEGGGEE DCENFQIGIK KLLNELKYSQ IETLKQKSLQ SGLNESEKKL LLSLLTAKQN
NT Sequence
ATGATTCCAT CCGACTTCAT TGACGAGCTT CTGGCCAAAA CCGATATTGT CGGCATCATC GACGAGCAGG TTCCGCTGAA AAAAGGCGGG GCGAACTATA TGGCGTGTTG CCCGTTCCAC AAGGAAAAAA CACCGTCGTT TTCGGTCAGT CCGACCAAGC AGTTTTATCA TTGTTTCAGT TGCGGGGCGC ATGGTTCGGC GATTGGTTTT GTGATGGAAC ATCAGGGACT GTCGTTTCCG GAGGCGGTTC AGTTCCTTGC CGACCGCGTA GGTATGATTG TGCCTAAAGT GCGCGGGCAG AACGATAATC CCGAAGTCCG TGCCGAGCGC AAGAAAAAAC AGCAGACGCT GGAAGAAACG ACGGCGGCGG CAGCTGATTT TTACGCGCAG CAGCTGAAAT TCAATCCGGC TGCGAAAGCT TATTTGGACA AACGCGGCTT GAGCGCGGAA GTCATCGCGC ATTATGGTTT GGGCTACGCG CCCGACGGCT GGCAGCCTTT GGCGCAAGTG TTCCAACCGT ATCCGAATAC CGCGTTGGTG GATACGGGGA TGGTGATTGA CAATGAGGGG CGGCATTACG ACCGCTTCCG CCATCGGATT ATGTTCCCCA TCCGCAATCC GCGCGGGCAG GTCATCGGTT TCGGCGGCAG GGTGCTGGAC GACTCGAAAC CGAAATATTT GAATTCGCCC GATACGCCTT TGTTCGATAA GGGGAAAAAC CTTTACGGCT TGTATGAGGG GCGTGCTGCT GTAAAAGAGG CAGAACGGAT TTTGGTGGTC GAAGGCTATA TGGACGTGGT CGCGCTGGCA CAGTTCGGCG TGGGCTACGG TGTGGCGGCC TTGGGCACGG CGACGACGGC GGAACACGTC AAAATCCTGA TGCGGCAGGC GGACAGTATT TATTTCTGTT TCGACGGCGA CAGCGCGGGG CGAAAAGCGG CTTGGCGCGC GCTGGAAAAC GCGCTGCCGC AGTTGAAAGA CGACAAATCG CTGCATTTTT TATTCCTGCC GGAAGAACAC GACCCCGACA GCTACATCCG CGCCTACGGC AAAGCGCAAT TTGAAGACGC GCTTCTGAAT CAAAGCAAGC CTTTGTCGGA ATATTTCTGG GAACACCTTT CAGACGGCAT TCATCTCAAT ACGCAGGAAG GCAAGGCGGA ATTGGTGAAA ACCAGTTCGC CGCTTTTGGT GCAGATTACC GCACCGGCAT TGGCTTATTT GTTAAAACAA CGGCTTAGCG AGCTGGTCGG CATCGACCCC GACAACCTCG CGCAACTGCT CGGACAGGAA GCGCCAAAGC GGCACGTCAA ACAAAAAAAC TACAAACTGC CTCCGATTTC CGTCAAACAG CCCGTTATGC CGACATTGGT GCAACGGCAA ATCCGCAGCC TCTTGATAAA TCCGGATTGG GCTGCATATA TAGACCTGCC CGATTATCTG GCGTTGGACG GCGATTTCGC CTGCCTTGCC AACCTCGCCG AAACCATTAA AAACCATCCT TCCGTGCCGG CAACCGCACA GGTTTTGGAA CATATGCGCG GTTCGCCCTA CGAAGAAACA ATCGACCGCA TCTTCCGGTC GGCCCTTCAA TCGGAAGAAA TGGAAGGCGG CGGCGAAGAA GATTGCGAAA ACTTCCAAAT CGGCATCAAA AAACTGCTCA ATGAGTTAAA ATACAGCCAA ATTGAAACAT TAAAACAAAA AAGCCTGCAA TCCGGCTTAA ATGAAAGCGA GAAAAAACTT TTGCTGTCGC TGCTGACCGC AAAACAAAAT TGA
Details for NegoA.00153.a.B1.GE39953
HARVESTED ON: data unavailable
SEQUENCED ON: 3/1/2016
EXPECTED MW: 67kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMI PSDFIDELLA KTDIVGIIDE QVPLKKGGAN YMACCPFHKE KTPSFSVSPT KQFYHCFSCG AHGSAIGFVM EHQGLSFPEA VQFLADRVGM IVPKVRGQND NPEVRAERKK KQQTLEETTA AAADFYAQQL KFNPAAKAYL DKRGLSAEVI AHYGLGYAPD GWQPLAQVFQ PYPNTALVDT GMVIDNEGRH YDRFRHRIMF PIRNPRGQVI GFGGRVLDDS KPKYLNSPDT PLFDKGKNLY GLYEGRAAVK EAERILVVEG YMDVVALAQF GVGYGVAALG TATTAEHVKI LMRQADSIYF CFDGDSAGRK AAWRALENAL PQLKDDKSLH FLFLPEEHDP DSYIRAYGKA QFEDALLNQS KPLSEYFWEH LSDGIHLNTQ EGKAELVKTS SPLLVQITAP ALAYLLKQRL SELVGIDPDN LAQLLGQEAP KRHVKQKNYK LPPISVKQPV MPTLVQRQIR SLLINPDWAA YIDLPDYLAL DGDFACLANL AETIKNHPSV PATAQVLEHM RGSPYEETID RIFRSALQSE EMEGGGEEDC ENFQIGIKKL LNELKYSQIE TLKQKSLQSG LNESEKKLLL SLLTAKQN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc catccgactt cattgacgag cttctggcca aaaccgatat tgtcggcatc atcgacgagc aggttccgct gaaaaaaggc ggggcgaact atatggcgtg ttgcccgttc cacaaggaaa aaacaccgtc gttttcggtc agtccgacca agcagtttta tcattgtttc agttgcgggg cgcatggttc ggcgattggt tttgtgatgg aacatcaggg actgtcgttt ccggaggcgg ttcagttcct tgccgaccgc gtaggtatga ttgtgcctaa agtgcgcggg cagaacgata atcccgaagt ccgtgccgag cgcaagaaaa aacagcagac gctggaagaa acgacggcgg cggcagctga tttttacgcg cagcagctga aattcaatcc ggctgcgaaa gcttatttgg acaaacgcgg cttgagcgcg gaagtcatcg cgcattatgg tttgggctac gcgcccgacg gctggcagcc tttggcgcaa gtgttccaac cgtatccgaa taccgcgttg gtggatacgg ggatggtgat tgacaatgag gggcggcatt acgaccgctt ccgccatcgg attatgttcc ccatccgcaa tccgcgcggg caggtcatcg gtttcggcgg cagggtgctg gacgactcga aaccgaaata tttgaattcg cccgatacgc ctttgttcga taaggggaaa aacctttacg gcttgtatga ggggcgtgct gctgtaaaag aggcagaacg gattttggtg gtcgaaggct atatggacgt ggtcgcgctg gcacagttcg gcgtgggcta cggtgtggcg gccttgggca cggcgacgac ggcggaacac gtcaaaatcc tgatgcggca ggcggacagt atttatttct gtttcgacgg cgacagcgcg gggcgaaaag cggcttggcg cgcgctggaa aacgcgctgc cgcagttgaa agacgacaaa tcgctgcatt ttttattcct gccggaagaa cacgaccccg acagctacat ccgcgcctac ggcaaagcgc aatttgaaga cgcgcttctg aatcaaagca agcctttgtc ggaatatttc tgggaacacc tttcagacgg cattcatctc aatacgcagg aaggcaaggc ggaattggtg aaaaccagtt cgccgctttt ggtgcagatt accgcaccgg cattggctta tttgttaaaa caacggctta gcgagctggt cggcatcgac cccgacaacc tcgcgcaact gctcggacag gaagcgccaa agcggcacgt caaacaaaaa aactacaaac tgcctccgat ttccgtcaaa cagcccgtta tgccgacatt ggtgcaacgg caaatccgca gcctcttgat aaatccggat tgggctgcat atatagacct gcccgattat ctggcgttgg acggcgattt cgcctgcctt gccaacctcg ccgaaaccat taaaaaccat ccttccgtgc cggcaaccgc acaggttttg gaacatatgc gcggttcgcc ctacgaagaa acaatcgacc gcatcttccg gtcggccctt caatcggaag aaatggaagg cggcggcgaa gaagattgcg aaaacttcca aatcggcatc aaaaaactgc tcaatgagtt aaaatacagc caaattgaaa cattaaaaca aaaaagcctg caatccggct taaatgaaag cgagaaaaaa cttttgctgt cgctgctgac cgcaaaacaa aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat