NegoA.00120.a

GTPase obg

CENTER ID:	NegoA.00120.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00120.a.B1.GE39949	full length		1	384

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00120.a.B1.PW37918	full length		1	384

Molecular models

METHOD	RESULTS
de-novo with evolutionary constraints	ModelArchive_ma-8kiqr

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.2427
UniProt:	B4RQP4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKFIDEAKIE VAAGKGGNGA TSFRREKFVP RGGPDGGDGG KGGSVWAEAD ENTNTLVEYR FVKRYQAKNG EKGHGSDRYG AGADDIVLKM PVGTLIRDLD TDEIVADLTY HGQRVCLAKG GKGGLGNIHF KSSVNRAPKQ STPGEEGETR SLQLELKVLA DVGLLGMPNA GKSTLITAVS AARPKIANYP FTTLHPNLGV VRIDENHSFV MADIPGLIEG AAEGAGLGHR FLKHLSRTGL LLHVVDLAPF DETVNPAEEA LAIINELRKY DEELYGKPRW LVLNKLDMLD EEEARARTAA FLEAVGWDYP EPDDRFQFDM ETPRLFQISA LTHQGTQELV HQINQYLAEK KRIEAEKAEA EKAAANVEII EQQPKTDTGV FKPE

NT Sequence

ATGAAATTCA TCGACGAAGC AAAAATCGAA GTCGCCGCAG GCAAAGGCGG TAATGGCGCA ACCAGTTTCC GCCGCGAAAA ATTCGTACCC CGCGGCGGCC CGGACGGTGG CGACGGCGGC AAAGGCGGCA GCGTCTGGGC AGAAGCCGAC GAAAACACCA ACACCCTCGT CGAATACCGC TTCGTCAAAC GCTACCAAGC CAAAAACGGC GAAAAAGGCC ACGGTTCCGA CCGCTACGGC GCAGGTGCGG ACGACATTGT CCTCAAAATG CCCGTCGGCA CCCTTATCCG CGACCTCGAC ACCGACGAAA TCGTTGCCGA CCTCACCTAC CACGGACAGC GCGTCTGCCT CGCCAAAGGC GGCAAAGGCG GCTTGGGCAA CATCCACTTC AAATCCTCCG TCAACCGCGC CCCAAAACAA TCCACGCCCG GCGAAGAAGG CGAAACCCGT TCCCTGCAAC TCGAACTCAA AGTCCTCGCC GATGTCGGCT TGTTAGGCAT GCCCAACGCC GGCAAATCCA CCCTGATTAC CGCCGTCTCC GCCGCACGCC CCAAAATCGC CAACTACCCC TTCACCACCC TGCATCCGAA CTTGGGCGTG GTACGCATCG ACGAAAACCA CAGCTTCGTG ATGGCCGACA TCCCCGGTCT GATTGAAGGC GCGGCAGAAG GCGCAGGCCT CGGCCACCGC TTCCTCAAAC ACCTATCACG CACCGGTCTG CTGTTACACG TTGTCGATTT GGCACCCTTC GACGAAACCG TCAATCCCGC CGAAGAAGCA CTCGCCATCA TCAACGAATT ACGCAAATAC GACGAAGAAC TTTACGGCAA ACCGCGCTGG CTGGTGCTGA ACAAACTCGA TATGCTCGAC GAAGAAGAAG CCCGGGCGCG AACAGCCGCC TTCCTCGAAG CCGTCGGCTG GGACTACCCC GAACCTGATG ACCGTTTCCA ATTCGACATG GAAACCCCGC GCCTCTTCCA AATCAGCGCG CTGACACATC AAGGCACGCA GGAGTTGGTA CACCAAATCA ACCAATACCT GGCCGAGAAA AAACGTATCG AGGCTGAAAA AGCCGAAGCG GAGAAAGCAG CGGCAAATGT TGAAATTATC GAACAGCAGC CTAAAACGGA TACTGGCGTG TTTAAGCCGG AGTAA

Details for NegoA.00120.a.B1.GE39949

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK FIDEAKIEVA AGKGGNGATS FRREKFVPRG GPDGGDGGKG GSVWAEADEN TNTLVEYRFV KRYQAKNGEK GHGSDRYGAG ADDIVLKMPV GTLIRDLDTD EIVADLTYHG QRVCLAKGGK GGLGNIHFKS SVNRAPKQST PGEEGETRSL QLELKVLADV GLLGMPNAGK STLITAVSAA RPKIANYPFT TLHPNLGVVR IDENHSFVMA DIPGLIEGAA EGAGLGHRFL KHLSRTGLLL HVVDLAPFDE TVNPAEEALA IINELRKYDE ELYGKPRWLV LNKLDMLDEE EARARTAAFL EAVGWDYPEP DDRFQFDMET PRLFQISALT HQGTQELVHQ INQYLAEKKR IEAEKAEAEK AAANVEIIEQ QPK

Validated NT Sequence

ttttaggctg ctgttcgata atttcaacat ttgccgctgc tttctccgct tcggcttttt cagcctcgat acgttttttc tcggccaggt attggttgat ttggtgtacc aactcctgcg tgccttgatg tgtcagcgcg ctgatttgga agaggcgcgg ggtttccatg tcgaattgga aacggtcatc aggttcgggg tagtcccagc cgacggcttc gaggaaggcg gctgttcgcg cccgggcttc ttcttcgtcg agcatatcga gtttgttcag caccagccag cgcggtttgc cgtaaagttc ttcgtcgtat ttgcgtaatt cgttgatgat ggcgagtgct tcttcggcgg gattgacggt ttcgtcgaag ggtgccaaat cgacaacgtg taacagcaga ccggtgcgtg ataggtgttt gaggaagcgg tggccgaggc ctgcgccttc tgccgcgcct tcaatcaggc cggggatgtc ggccatcacg aagctgtggt tttcgtcgat gcgtaccacg cccaagttcg gatgcagggt ggtgaagggg tagttggcga ttttggggcg tgcggcggag acggcggtaa tcagggtgga tttgccggcg ttgggcatgc ctaacaagcc gacatcggcg aggactttga gttcgagttg cagggaacgg gtttcgcctt cttcgccggg cgtggattgt tttggggcgc ggttgacgga ggatttgaag tggatgttgc ccaagccgcc tttgccgcct ttggcgaggc agacgcgctg tccgtggtag gtgaggtcgg caacgatttc gtcggtgtcg aggtcgcgga taagggtgcc gacgggcatt ttgaggacaa tgtcgtccgc acctgcgccg tagcggtcgg aaccgtggcc tttttcgccg tttttggctt ggtagcgttt gacgaagcgg tattcgacga gggtgttggt gttttcgtcg gcttctgccc agacgctgcc gcctttgccg ccgtcgccac cgtccgggcc gccgcggggt acgaattttt cgcggcggaa actggttgcg ccattaccgc ctttgcctgc ggcgacttcg atttttgctt cgtcgatgaa tttcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat tcatcgacga agcaaaaatc gaagtcgccg caggcaaagg cggtaatggc gcaaccagtt tccgccgcga aaaattcgta ccccgcggcg gcccggacgg tggcgacggc ggcaaaggcg gcagcgtctg ggcagaagcc gacgaaaaca ccaacaccct cgtcgaatac cgcttcgtca aacgctacca agccaaaaac ggcgaaaaag gccacggttc cgaccgctac ggcgcaggtg cggacgacat tgtcctcaaa atgcccgtcg gcacccttat ccgcgacctc gacaccgacg aaatcgttgc cgacctcacc taccacggac agcgcgtctg cctcgccaaa ggcggcaaag gcggcttggg caacatccac ttcaaatcct ccgtcaaccg cgccccaaaa caatccacgc ccggcgaaga aggcgaaacc cgttccctgc aactcgaact caaagtcctc gccgatgtcg gcttgttagg catgcccaac gccggcaaat ccaccctgat taccgccgtc tccgccgcac gccccaaaat cgccaactac cccttcacca ccctgcatcc gaacttgggc gtggtacgca tcgacgaaaa ccacagcttc gtgatggccg acatccccgg tctgattgaa ggcgcggcag aaggcgcagg cctcggccac cgcttcctca aacacctatc acgcaccggt ctgctgttac acgttgtcga tttggcaccc ttcgacgaaa ccgtcaatcc cgccgaagaa gcactcgcca tcatcaacga attacgcaaa tacgacgaag aactttacgg caaaccgcgc tggctggtgc tgaacaaact cgatatgctc gacgaagaag aagcccgggc gcgaacagcc gccttcctcg aagccgtcgg ctgggactac cccgaacctg atgaccgttt ccaattcgac atggaaaccc cgcgcctctt ccaaatcagc gcgctgacac atcaaggcac gcaggagttg gtacaccaaa tcaaccaata cctggccgag aaaaaacgta tcgaggctga aaaagccgaa gcggagaaag cagcggcaaa tgttgaaatt atcgaacagc agcctaaaac ggatactggc gtgtttaagc cggagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for NegoA.00120.a.B1.PW37918

PURIFICATION DATe:	6/8/2016
CONCENTRATION:	29.5mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT TCATCGACGA AGCAAAAATC GAAGTCGCCG CAGGCAAAGG CGGTAATGGC GCAACCAGTT TCCGCCGCGA AAAATTCGTA CCCCGCGGCG GCCCGGACGG TGGCGACGGC GGCAAAGGCG GCAGCGTCTG GGCAGAAGCC GACGAAAACA CCAACACCCT CGTCGAATAC CGCTTCGTCA AACGCTACCA AGCCAAAAAC GGCGAAAAAG GCCACGGTTC CGACCGCTAC GGCGCAGGTG CGGACGACAT TGTCCTCAAA ATGCCCGTCG GCACCCTTAT CCGCGACCTC GACACCGACG AAATCGTTGC CGACCTCACC TACCACGGAC AGCGCGTCTG CCTCGCCAAA GGCGGCAAAG GCGGCTTGGG CAACATCCAC TTCAAATCCT CCGTCAACCG CGCCCCAAAA CAATCCACGC CCGGCGAAGA AGGCGAAACC CGTTCCCTGC AACTCGAACT CAAAGTCCTC GCCGATGTCG GCTTGTTAGG CATGCCCAAC GCCGGCAAAT CCACCCTGAT TACCGCCGTC TCCGCCGCAC GCCCCAAAAT CGCCAACTAC CCCTTCACCA CCCTGCATCC GAACTTGGGC GTGGTACGCA TCGACGAAAA CCACAGCTTC GTGATGGCCG ACATCCCCGG TCTGATTGAA GGCGCGGCAG AAGGCGCAGG CCTCGGCCAC CGCTTCCTCA AACACCTATC ACGCACCGGT CTGCTGTTAC ACGTTGTCGA TTTGGCACCC TTCGACGAAA CCGTCAATCC CGCCGAAGAA GCACTCGCCA TCATCAACGA ATTACGCAAA TACGACGAAG AACTTTACGG CAAACCGCGC TGGCTGGTGC TGAACAAACT CGATATGCTC GACGAAGAAG AAGCCCGGGC GCGAACAGCC GCCTTCCTCG AAGCCGTCGG CTGGGACTAC CCCGAACCTG ATGACCGTTT CCAATTCGAC ATGGAAACCC CGCGCCTCTT CCAAATCAGC GCGCTGACAC ATCAAGGCAC GCAGGAGTTG GTACACCAAA TCAACCAATA CCTGGCCGAG AAAAAACGTA TCGAGGCTGA AAAAGCCGAA GCGGAGAAAG CAGCGGCAAA TGTTGAAATT ATCGAACAGC AGCCTAAAAC GGATACTGGC GTGTTTAAGC CGGAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT