NegoA.00101.b
ferredoxin-NADP+ reductase
| CENTER ID: | NegoA.00101.b |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00101.b.B1.GE39948 | full length | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00101.b.B1.PW37917 | full length | 1 | 259 |
Structures
5TR9
DEPOSITED: 10/25/2016
DETERMINATION: XRay
CLONE: NegoA.00101.b.B1.GE39948
PROTEIN: NegoA.00101.b.B1.PW37917
5UFA
DEPOSITED: 1/4/2017
DETERMINATION: XRay
CLONE: NegoA.00101.b.B1.GE39948
PROTEIN: NegoA.00101.b.B1.PW37917
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.1231 |
| UniProt: | B4RLY3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAASPEAKFT EEKILWVKHH TPKLITFAIS RPESYRFKAG QFSRLGFYEG KGFIWRAYSV VSAEYADTLE YFAVLIQDGP MSALFAKMQQ GDTILLDKNA TGFLLPERFP DGKDLVMLCT GSGIAPFLSI LEQPEIRQRF DTVNLIHSVS FPEELIFNDR LAALSEHPLV GEYGHSFRFV PVTTRAANPS GLSGKRIPEL LKNNSIEQAL HTKLTPESTR FMICGNPEMV KDTFQTLLDM GYAMHRNRIP GQIMMENGF
NT Sequence
ATGGCAGCCT CGCCCGAAGC AAAATTCACC GAAGAAAAGA TTTTGTGGGT CAAACACCAC ACGCCGAAAC TCATCACTTT CGCCATCAGC CGTCCCGAAT CCTACCGTTT TAAAGCCGGA CAGTTCTCCC GACTCGGTTT CTACGAAGGT AAAGGCTTTA TTTGGCGTGC CTATTCCGTC GTTTCCGCAG AATATGCCGA CACGCTCGAA TATTTTGCCG TACTCATCCA AGACGGCCCC ATGTCGGCCC TTTTCGCCAA AATGCAACAG GGCGACACCA TCCTGCTCGA TAAAAATGCC ACTGGCTTTC TCCTGCCCGA ACGCTTCCCC GACGGCAAGG ATTTGGTGAT GCTCTGTACC GGCTCGGGCA TCGCCCCCTT CCTTTCCATT CTCGAACAAC CCGAAATCCG GCAGCGTTTC GATACCGTCA ACCTGATACA TTCCGTATCT TTTCCCGAAG AATTGATTTT CAACGACCGG CTGGCCGCAT TGTCCGAACA TCCCCTGGTA GGCGAATACG GACACTCGTT CCGTTTCGTC CCTGTTACCA CCCGTGCCGC CAACCCCTCG GGCTTAAGCG GAAAACGCAT TCCGGAACTC TTAAAAAACA ACAGCATCGA ACAGGCGCTG CATACCAAGC TCACCCCGGA ATCCACACGG TTTATGATTT GCGGTAACCC GGAAATGGTC AAAGACACTT TCCAAACGCT GCTCGATATG GGTTACGCCA TGCACCGCAA CCGCATTCCC GGTCAAATCA TGATGGAAAA CGGCTTCTAA
Details for NegoA.00101.b.B1.GE39948
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 101 |
Validated AA Sequence
MAHHHHHHMA ASPEAKFTEE KILWVKHHTP KLITFAISRP ESYRFKAGQF SRLGFYEGKG FIWRAYSVVS AEYADTLEYF AVLIQDGPMS ALFAKMQQGD TILLDKNATG FLLPERFPDG KDLVMLCTGS GIAPFLSILE QPEIRQRFDT VNLIHSVSFP EELIFNDRLA ALSEHPLVGE YGHSFRFVPV TTRAANPSGL SGKRIPELLK NNSIEQALHT KLTPESTRFM ICGNPEMVKD TFQTLLDMGY AMHRNRIXXX XHXXNGXXVS K
Validated NT Sequence
tcttactcac ttnnnnnccg ttttnnncat gnnnnnnnnn nnnaatgcgg ttgcggtgca tggcgtaacc catatcgagc agngtttgga aagtgtcttt gaccatttcc gggttaccgc aaatcataaa ccgtgtggat tccggggtga gcttggtatg cagcgcctgt tcgatgctgt tgttttttaa gagttccgga atgcgttttc cgcttaagcc cgaggggttg gcggcacggg tggtaacagg gacgaaacgg aacgagtgtc cgtattcgcc taccagggga tgttcggaca atgcggccag ccggtcgttg aaaatcaatt cttcgggaaa agatacggaa tgtatcaggt tgacggtatc gaaacgctgc cggatttcgg gttgttcgag aatggaaagg aagggggcga tgcccgagcc ggtacagagc atcaccaaat ccttgccgtc ggggaagcgt tcgggcagga gaaagccagt ggcattttta tcgagcagga tggtgtcgcc ctgttgcatt ttggcgaaaa gggccgacat ggggccgtct tggatgagta cggcaaaata ttcgagcgtg tcggcatatt ctgcggaaac gacggaatag gcacgccaaa taaagccttt accttcgtag aaaccgagtc gggagaactg tccggcttta aaacggtagg attcgggacg gctgatggcg aaagtgatga gtttcggcgt gtggtgtttg acccacaaaa tcttttcttc ggtgaatttt gcttcgggcg aggctgccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMA ASPEAKFTEE KILWVKHHTP KLITFAISRP ESYRFKAGQF SRLGFYEGKG FIWRAYSVVS AEYADTLEYF AVLIQDGPMS ALFAKMQQGD TILLDKNATG FLLPERFPDG KDLVMLCTGS GIAPFLSILE QPEIRQRFDT VNLIHSVSFP EELIFNDRLA ALSEHPLVGE YGHSFRFVPV TTRAANPSGL SGKRIPELLK NNSIEQALHT KLTPESTRFM ICGNPEMVKD TFQTLLDMGY AMHRNRIPGQ IMMENGF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag cctcgcccga agcaaaattc accgaagaaa agattttgtg ggtcaaacac cacacgccga aactcatcac tttcgccatc agccgtcccg aatcctaccg ttttaaagcc ggacagttct cccgactcgg tttctacgaa ggtaaaggct ttatttggcg tgcctattcc gtcgtttccg cagaatatgc cgacacgctc gaatattttg ccgtactcat ccaagacggc cccatgtcgg cccttttcgc caaaatgcaa cagggcgaca ccatcctgct cgataaaaat gccactggct ttctcctgcc cgaacgcttc cccgacggca aggatttggt gatgctctgt accggctcgg gcatcgcccc cttcctttcc attctcgaac aacccgaaat ccggcagcgt ttcgataccg tcaacctgat acattccgta tcttttcccg aagaattgat tttcaacgac cggctggccg cattgtccga acatcccctg gtaggcgaat acggacactc gttccgtttc gtccctgtta ccacccgtgc cgccaacccc tcgggcttaa gcggaaaacg cattccggaa ctcttaaaaa acaacagcat cgaacaggcg ctgcatacca agctcacccc ggaatccaca cggtttatga tttgcggtaa cccggaaatg gtcaaagaca ctttccaaac gctgctcgat atgggttacg ccatgcaccg caaccgcatt cccggtcaaa tcatgatgga aaacggcttc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NegoA.00101.b.B1.PW37917
| PURIFICATION DATe: | 6/8/2016 |
| CONCENTRATION: | 37mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 101 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA ASPEAKFTEE KILWVKHHTP KLITFAISRP ESYRFKAGQF SRLGFYEGKG FIWRAYSVVS AEYADTLEYF AVLIQDGPMS ALFAKMQQGD TILLDKNATG FLLPERFPDG KDLVMLCTGS GIAPFLSILE QPEIRQRFDT VNLIHSVSFP EELIFNDRLA ALSEHPLVGE YGHSFRFVPV TTRAANPSGL SGKRIPELLK NNSIEQALHT KLTPESTRFM ICGNPEMVKD TFQTLLDMGY AMHRNRIXXX XHXXNGXXVS K
Validated NT Sequence
tcttactcac ttnnnnnccg ttttnnncat gnnnnnnnnn nnnaatgcgg ttgcggtgca tggcgtaacc catatcgagc agngtttgga aagtgtcttt gaccatttcc gggttaccgc aaatcataaa ccgtgtggat tccggggtga gcttggtatg cagcgcctgt tcgatgctgt tgttttttaa gagttccgga atgcgttttc cgcttaagcc cgaggggttg gcggcacggg tggtaacagg gacgaaacgg aacgagtgtc cgtattcgcc taccagggga tgttcggaca atgcggccag ccggtcgttg aaaatcaatt cttcgggaaa agatacggaa tgtatcaggt tgacggtatc gaaacgctgc cggatttcgg gttgttcgag aatggaaagg aagggggcga tgcccgagcc ggtacagagc atcaccaaat ccttgccgtc ggggaagcgt tcgggcagga gaaagccagt ggcattttta tcgagcagga tggtgtcgcc ctgttgcatt ttggcgaaaa gggccgacat ggggccgtct tggatgagta cggcaaaata ttcgagcgtg tcggcatatt ctgcggaaac gacggaatag gcacgccaaa taaagccttt accttcgtag aaaccgagtc gggagaactg tccggcttta aaacggtagg attcgggacg gctgatggcg aaagtgatga gtttcggcgt gtggtgtttg acccacaaaa tcttttcttc ggtgaatttt gcttcgggcg aggctgccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMA ASPEAKFTEE KILWVKHHTP KLITFAISRP ESYRFKAGQF SRLGFYEGKG FIWRAYSVVS AEYADTLEYF AVLIQDGPMS ALFAKMQQGD TILLDKNATG FLLPERFPDG KDLVMLCTGS GIAPFLSILE QPEIRQRFDT VNLIHSVSFP EELIFNDRLA ALSEHPLVGE YGHSFRFVPV TTRAANPSGL SGKRIPELLK NNSIEQALHT KLTPESTRFM ICGNPEMVKD TFQTLLDMGY AMHRNRIPGQ IMMENGF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG CCTCGCCCGA AGCAAAATTC ACCGAAGAAA AGATTTTGTG GGTCAAACAC CACACGCCGA AACTCATCAC TTTCGCCATC AGCCGTCCCG AATCCTACCG TTTTAAAGCC GGACAGTTCT CCCGACTCGG TTTCTACGAA GGTAAAGGCT TTATTTGGCG TGCCTATTCC GTCGTTTCCG CAGAATATGC CGACACGCTC GAATATTTTG CCGTACTCAT CCAAGACGGC CCCATGTCGG CCCTTTTCGC CAAAATGCAA CAGGGCGACA CCATCCTGCT CGATAAAAAT GCCACTGGCT TTCTCCTGCC CGAACGCTTC CCCGACGGCA AGGATTTGGT GATGCTCTGT ACCGGCTCGG GCATCGCCCC CTTCCTTTCC ATTCTCGAAC AACCCGAAAT CCGGCAGCGT TTCGATACCG TCAACCTGAT ACATTCCGTA TCTTTTCCCG AAGAATTGAT TTTCAACGAC CGGCTGGCCG CATTGTCCGA ACATCCCCTG GTAGGCGAAT ACGGACACTC GTTCCGTTTC GTCCCTGTTA CCACCCGTGC CGCCAACCCC TCGGGCTTAA GCGGAAAACG CATTCCGGAA CTCTTAAAAA ACAACAGCAT CGAACAGGCG CTGCATACCA AGCTCACCCC GGAATCCACA CGGTTTATGA TTTGCGGTAA CCCGGAAATG GTCAAAGACA CTTTCCAAAC GCTGCTCGAT ATGGGTTACG CCATGCACCG CAACCGCATT CCCGGTCAAA TCATGATGGA AAACGGCTTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT