NegoA.00101.a
ferredoxin-NADP+ reductase
| CENTER ID: | NegoA.00101.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00101.a.B1.GE39947 | full length | 1 | 258 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00101.a.B1.PS37940 | full length | 1 | 258 |
Structures
5THX
DEPOSITED: 9/30/2016
DETERMINATION: XRay
CLONE: NegoA.00101.a.B1.GE39947
PROTEIN: NegoA.00101.a.B1.PS37940
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.1304 |
| UniProt: | B4RM59 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAFNTQKVL SVHHWTDAYF TFTCIRDESL RFENGQFVMV GLMADGKPLM RAYSVASANW EEHLEFFSIK VQDGPLTSRL QHLKVGDEVL ISKKPTGTLV ACDLNPGKHL YLLSTGTGIA PFLSITKDPE IYEQFEKIIL VHGVRYKKDL AYYDRFTKEL PEHEYLGDLV KEKLIYYPIV SREEFEHRGR LTDLMVSGKL FEDIGLPKIN PQDDRAMLCG SPAMLKDTCK VLDDFGLTVS PKTGVRGDYL IERAFVDQ
NT Sequence
ATGGCAGCAT TCAATACCCA AAAAGTATTG TCCGTACACC ACTGGACAGA CGCATATTTT ACCTTTACCT GCATCCGCGA CGAATCGTTG CGCTTTGAAA ACGGACAGTT CGTCATGGTC GGGCTGATGG CGGACGGCAA GCCGCTGATG CGCGCATACA GCGTCGCCTC CGCCAACTGG GAAGAACACC TCGAATTTTT CAGCATTAAA GTCCAAGACG GCCCGCTGAC CAGCCGCCTG CAACACCTCA AAGTCGGCGA CGAAGTGTTA ATCAGCAAAA AACCGACCGG AACTCTAGTT GCCTGCGATC TGAATCCGGG CAAACACCTT TACCTGTTGA GTACCGGTAC CGGCATCGCC CCTTTCTTGA GCATCACCAA AGACCCCGAA ATTTACGAAC AGTTTGAAAA AATCATCCTT GTACACGGCG TGCGCTACAA AAAGGATTTG GCATACTACG ACCGCTTTAC CAAAGAATTG CCCGAACACG AATACCTCGG CGACTTGGTT AAAGAAAAAC TGATTTACTA CCCGATTGTT TCCCGCGAAG AATTCGAACA CCGCGGCCGC CTGACCGACC TGATGGTAAG CGGCAAACTG TTTGAAGACA TCGGCCTGCC CAAAATCAAC CCCCAAGACG ACCGTGCCAT GCTGTGCGGC AGCCCTGCGA TGCTGAAAGA CACCTGCAAA GTTTTGGACG ATTTCGGTCT GACAGTCTCC CCGAAAACCG GCGTGCGCGG CGACTACCTG ATTGAGCGCG CGTTTGTGGA TCAATAA
Details for NegoA.00101.a.B1.GE39947
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA AFNTQKVLSV HHWTDAYFTF TCIRDESLRF ENGQFVMVGL MADGKPLMRA YSVASANWEE HLEFFSIKVQ DGPLTSRLQH LKVGDEVLIS KKPTGTLVAG DLNPGKHLYL LSTGTGIAPF LSITKDPEIY EQFEKIILVH GVRYKKDLAY YDRFTKELPE HEYLGDLVKE KLIYYPIVSR EEFEHRGRLT DLMVSGKLFE DIGLPKINPQ DDRAMLCGSP AMLKDTCKVL DDFGLTVSPK TGVRGDXXXE RAFVDQ
Validated NT Sequence
ttactcactt attgatccac aaacgcgcgc tcnnnnnnnt agtcgccgcg cacgccggtt ttcggggaga ctgtcagacc gaaatcgtcc aaaactttgc aggtatcttt cagcatcgcg gggctgccgc acagcatcgc gcggtcgtct tgcgggttga ttttgggcag gccgatgtct tcaaacagtt tgccgcttac catcaggtcg gtcaggcggc cgcggtgttc gaattcttct cgggaaacaa tcgggtagta aatcagtttt tctttaacca agtcgccgag gtattcgtgt tcgggcaatt ctttggtaaa gcggtcgtag tacgccaaat cttttttgta gcgcacgccg tgtacgagga tgattttttc aaattgctcg taaatctcgg ggtctttggt gatgctcaag aaaggggcga tgccggtacc ggtgctcaac aagtaaaggt gtttgcccgg attcaggtcg ccggcaacca gagttccggt cggttttttg ctgattaaca cttcgtcgcc gactttgagg tgttgcaggc ggctggtcag cgggccgtct tggactttaa tgctgaaaaa ttcgaggtgt tcttcccagt tggcggaggc gacgctgtat gcgcgcatca gcggcttgcc gtccgccatc agcccgacca tgacgaactg tccgttttca aagcgcaacg attcgtcgcg gatgcaggta aaggtaaaat atgcgtctgt ccagtggtgt acggacaata ctttttgggt attgaatgct gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMA AFNTQKVLSV HHWTDAYFTF TCIRDESLRF ENGQFVMVGL MADGKPLMRA YSVASANWEE HLEFFSIKVQ DGPLTSRLQH LKVGDEVLIS KKPTGTLVAC DLNPGKHLYL LSTGTGIAPF LSITKDPEIY EQFEKIILVH GVRYKKDLAY YDRFTKELPE HEYLGDLVKE KLIYYPIVSR EEFEHRGRLT DLMVSGKLFE DIGLPKINPQ DDRAMLCGSP AMLKDTCKVL DDFGLTVSPK TGVRGDYLIE RAFVDQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag cattcaatac ccaaaaagta ttgtccgtac accactggac agacgcatat tttaccttta cctgcatccg cgacgaatcg ttgcgctttg aaaacggaca gttcgtcatg gtcgggctga tggcggacgg caagccgctg atgcgcgcat acagcgtcgc ctccgccaac tgggaagaac acctcgaatt tttcagcatt aaagtccaag acggcccgct gaccagccgc ctgcaacacc tcaaagtcgg cgacgaagtg ttaatcagca aaaaaccgac cggaactcta gttgcctgcg atctgaatcc gggcaaacac ctttacctgt tgagtaccgg taccggcatc gcccctttct tgagcatcac caaagacccc gaaatttacg aacagtttga aaaaatcatc cttgtacacg gcgtgcgcta caaaaaggat ttggcatact acgaccgctt taccaaagaa ttgcccgaac acgaatacct cggcgacttg gttaaagaaa aactgattta ctacccgatt gtttcccgcg aagaattcga acaccgcggc cgcctgaccg acctgatggt aagcggcaaa ctgtttgaag acatcggcct gcccaaaatc aacccccaag acgaccgtgc catgctgtgc ggcagccctg cgatgctgaa agacacctgc aaagttttgg acgatttcgg tctgacagtc tccccgaaaa ccggcgtgcg cggcgactac ctgattgagc gcgcgtttgt ggatcaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for NegoA.00101.a.B1.PS37940
| PURIFICATION DATe: | 6/8/2016 |
| CONCENTRATION: | 40.9mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 21 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA AFNTQKVLSV HHWTDAYFTF TCIRDESLRF ENGQFVMVGL MADGKPLMRA YSVASANWEE HLEFFSIKVQ DGPLTSRLQH LKVGDEVLIS KKPTGTLVAG DLNPGKHLYL LSTGTGIAPF LSITKDPEIY EQFEKIILVH GVRYKKDLAY YDRFTKELPE HEYLGDLVKE KLIYYPIVSR EEFEHRGRLT DLMVSGKLFE DIGLPKINPQ DDRAMLCGSP AMLKDTCKVL DDFGLTVSPK TGVRGDXXXE RAFVDQ
Validated NT Sequence
ttactcactt attgatccac aaacgcgcgc tcnnnnnnnt agtcgccgcg cacgccggtt ttcggggaga ctgtcagacc gaaatcgtcc aaaactttgc aggtatcttt cagcatcgcg gggctgccgc acagcatcgc gcggtcgtct tgcgggttga ttttgggcag gccgatgtct tcaaacagtt tgccgcttac catcaggtcg gtcaggcggc cgcggtgttc gaattcttct cgggaaacaa tcgggtagta aatcagtttt tctttaacca agtcgccgag gtattcgtgt tcgggcaatt ctttggtaaa gcggtcgtag tacgccaaat cttttttgta gcgcacgccg tgtacgagga tgattttttc aaattgctcg taaatctcgg ggtctttggt gatgctcaag aaaggggcga tgccggtacc ggtgctcaac aagtaaaggt gtttgcccgg attcaggtcg ccggcaacca gagttccggt cggttttttg ctgattaaca cttcgtcgcc gactttgagg tgttgcaggc ggctggtcag cgggccgtct tggactttaa tgctgaaaaa ttcgaggtgt tcttcccagt tggcggaggc gacgctgtat gcgcgcatca gcggcttgcc gtccgccatc agcccgacca tgacgaactg tccgttttca aagcgcaacg attcgtcgcg gatgcaggta aaggtaaaat atgcgtctgt ccagtggtgt acggacaata ctttttgggt attgaatgct gccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMA AFNTQKVLSV HHWTDAYFTF TCIRDESLRF ENGQFVMVGL MADGKPLMRA YSVASANWEE HLEFFSIKVQ DGPLTSRLQH LKVGDEVLIS KKPTGTLVAC DLNPGKHLYL LSTGTGIAPF LSITKDPEIY EQFEKIILVH GVRYKKDLAY YDRFTKELPE HEYLGDLVKE KLIYYPIVSR EEFEHRGRLT DLMVSGKLFE DIGLPKINPQ DDRAMLCGSP AMLKDTCKVL DDFGLTVSPK TGVRGDYLIE RAFVDQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG CATTCAATAC CCAAAAAGTA TTGTCCGTAC ACCACTGGAC AGACGCATAT TTTACCTTTA CCTGCATCCG CGACGAATCG TTGCGCTTTG AAAACGGACA GTTCGTCATG GTCGGGCTGA TGGCGGACGG CAAGCCGCTG ATGCGCGCAT ACAGCGTCGC CTCCGCCAAC TGGGAAGAAC ACCTCGAATT TTTCAGCATT AAAGTCCAAG ACGGCCCGCT GACCAGCCGC CTGCAACACC TCAAAGTCGG CGACGAAGTG TTAATCAGCA AAAAACCGAC CGGAACTCTA GTTGCCTGCG ATCTGAATCC GGGCAAACAC CTTTACCTGT TGAGTACCGG TACCGGCATC GCCCCTTTCT TGAGCATCAC CAAAGACCCC GAAATTTACG AACAGTTTGA AAAAATCATC CTTGTACACG GCGTGCGCTA CAAAAAGGAT TTGGCATACT ACGACCGCTT TACCAAAGAA TTGCCCGAAC ACGAATACCT CGGCGACTTG GTTAAAGAAA AACTGATTTA CTACCCGATT GTTTCCCGCG AAGAATTCGA ACACCGCGGC CGCCTGACCG ACCTGATGGT AAGCGGCAAA CTGTTTGAAG ACATCGGCCT GCCCAAAATC AACCCCCAAG ACGACCGTGC CATGCTGTGC GGCAGCCCTG CGATGCTGAA AGACACCTGC AAAGTTTTGG ACGATTTCGG TCTGACAGTC TCCCCGAAAA CCGGCGTGCG CGGCGACTAC CTGATTGAGC GCGCGTTTGT GGATCAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT