NegoA.00097.a
pantoate-beta-alanine ligase
| CENTER ID: | NegoA.00097.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00097.a.B1.GE39946 | full length | 1 | 278 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00097.a.B1.PS37929 | full length | 1 | 278 |
Structures
5KWV
DEPOSITED: 7/19/2016
DETERMINATION: XRay
CLONE: NegoA.00097.a.B1.GE39946
PROTEIN: NegoA.00097.a.B1.PS37929
5UCR
DEPOSITED: 12/22/2016
DETERMINATION: XRay
CLONE: NegoA.00097.a.B1.GE39946
PROTEIN: NegoA.00097.a.B1.PS37929
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.660 |
| UniProt: | B4RKE7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIIHTIREL RTWRENTGKV AFVPTMGNLH EGHLALVREA RKRADNVVVS IFVNRLQFGQ GEDFDKYPRT LQQDADKLAA EGVAVVFAPD EKELYPNVEQ RYNVEPPHLQ NELCGKFRPG HFRGVATVVS KLFNIVLPDV ACFGKKDYQQ LAVIKGLTED LNFDIEIVPV DTGRAADGLA LSSRNRYLSV GERAEAPRLY RELQAVAESL KQGGLDYAGL ERQAADHLTA AGWLVDYVEI RRADTLEMAR AGDKKLVVLA AARLGTTRLI DNVEVGLP
NT Sequence
ATGCAAATCA TACATACCAT TCGAGAACTG CGCACGTGGC GTGAAAATAC GGGAAAGGTG GCATTTGTGC CGACCATGGG CAATCTGCAT GAAGGGCATC TTGCCCTTGT TCGCGAAGCC AGAAAACGCG CCGACAACGT TGTTGTCAGC ATATTCGTCA ACCGCTTGCA ATTCGGTCAG GGCGAGGATT TCGACAAATA TCCGCGTACT TTGCAACAGG ATGCGGACAA ACTTGCCGCC GAAGGCGTTG CCGTTGTTTT CGCGCCCGAT GAGAAAGAAC TCTATCCGAA CGTAGAACAG CGTTACAACG TCGAACCGCC CCATTTGCAA AACGAATTGT GCGGCAAATT CCGCCCCGGC CATTTTCGCG GTGTGGCGAC GGTGGTGTCC AAGCTGTTCA ATATCGTTTT GCCGGATGTT GCCTGTTTCG GCAAAAAAGA TTACCAGCAG CTTGCCGTGA TTAAAGGTTT GACCGAGGAT TTGAATTTCG ATATCGAAAT TGTCCCCGTC GATACGGGGC GGGCGGCGGA CGGTTTGGCA CTCTCCAGCC GCAACCGGTA CTTGAGCGTT GGGGAACGCG CCGAAGCACC GCGCCTGTAC CGCGAGCTGC AGGCTGTTGC CGAATCGCTG AAGCAGGGCG GTTTGGATTA TGCGGGTTTG GAGCGGCAGG CTGCCGACCA TTTGACCGCT GCGGGCTGGC TGGTCGATTA TGTGGAAATC CGCCGCGCCG ATACGCTCGA AATGGCGCGG GCGGGAGATA AGAAACTGGT GGTCTTGGCC GCCGCCCGTC TGGGGACGAC GCGTCTGATT GACAATGTGG AAGTCGGCCT GCCTTAG
Details for NegoA.00097.a.B1.GE39946
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MXXXHTIXXL RTWRENIGKV AFVPTMGNLH EGHLALVREA RKRADNVVVS IFVNRLQXXQ GEDFDKYPRT LQQDADKLAA EGVAVVFAPD EKELYPNVEQ RYNVEPPHLQ NELCGKFRPG HFRGVATVVS KLFNIVLPDV ACFGKKDYQQ LAVIKGLTED LNFDIEIVPV DTGRAADGLA LSSRNRYLSV GERAEAPRLY RELQAVAESL KQAGLDYAGL ERQAADHLTA AGWLVDYVEI RRADTLEMAR AGDKKLVVLA AARLGTTRLX XXVEVGLP
Validated NT Sequence
tcttactcac ttaaggcagg ccgacttcca catnnncnnt cagacgcgtc gtccccagac gggcggcggc caagaccacc agtttcttat ctcccgcccg cgccatttcg agcgtatcgg cgcggcggat ttccacataa tcgaccagcc agcccgcagc ggtcaaatgg tcggcagcct gccgctccaa acccgcataa tccaaaccgg cctgcttcag cgattcggca acagcctgca gctcgcggta caggcgcggt gcttcggcgc gttccccaac gctcaagtac cggttgcggc tggagagtgc caaaccgtcc gccgcccgcc ccgtatcgac ggggacaatt tcgatatcga aattcaaatc ctcggtcaaa cctttaatca cggcaagctg ctggtaatct tttttgccga aacaggcaac atccggcaaa acgatattga acagcttgga caccaccgtc gccacgccgc gaaaatggcc ggggcggaat ttgccgcaca attcgttttg caaatggggc ggttcgacgt tgtaacgctg ttccacgttc ggatagagtt ctttctcatc gggcgcgaaa acaacggcaa cgccttcggc ggcaagtttg tccgcatcct gttgcaaagt acgcggatat ttgtcgaaat cctcgccctg acnnaattgc aagcggttga cgaatatgct gacaacaacg ttgtcggcgc gttttctggc ttcgcgaaca agggcaagat gcccttcatg cagattgccc atggtcggca caaatgccac ctttcctata ttttcacgcc acgtgcgcag ttnnngaatg gtatgtnnna ntnncatatg gtggtggtgg tggtgannnn nggta
Expected Protein Sequence
MAHHHHHHMQ IIHTIRELRT WRENTGKVAF VPTMGNLHEG HLALVREARK RADNVVVSIF VNRLQFGQGE DFDKYPRTLQ QDADKLAAEG VAVVFAPDEK ELYPNVEQRY NVEPPHLQNE LCGKFRPGHF RGVATVVSKL FNIVLPDVAC FGKKDYQQLA VIKGLTEDLN FDIEIVPVDT GRAADGLALS SRNRYLSVGE RAEAPRLYRE LQAVAESLKQ GGLDYAGLER QAADHLTAAG WLVDYVEIRR ADTLEMARAG DKKLVVLAAA RLGTTRLIDN VEVGLP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa tcatacatac cattcgagaa ctgcgcacgt ggcgtgaaaa tacgggaaag gtggcatttg tgccgaccat gggcaatctg catgaagggc atcttgccct tgttcgcgaa gccagaaaac gcgccgacaa cgttgttgtc agcatattcg tcaaccgctt gcaattcggt cagggcgagg atttcgacaa atatccgcgt actttgcaac aggatgcgga caaacttgcc gccgaaggcg ttgccgttgt tttcgcgccc gatgagaaag aactctatcc gaacgtagaa cagcgttaca acgtcgaacc gccccatttg caaaacgaat tgtgcggcaa attccgcccc ggccattttc gcggtgtggc gacggtggtg tccaagctgt tcaatatcgt tttgccggat gttgcctgtt tcggcaaaaa agattaccag cagcttgccg tgattaaagg tttgaccgag gatttgaatt tcgatatcga aattgtcccc gtcgatacgg ggcgggcggc ggacggtttg gcactctcca gccgcaaccg gtacttgagc gttggggaac gcgccgaagc accgcgcctg taccgcgagc tgcaggctgt tgccgaatcg ctgaagcagg gcggtttgga ttatgcgggt ttggagcggc aggctgccga ccatttgacc gctgcgggct ggctggtcga ttatgtggaa atccgccgcg ccgatacgct cgaaatggcg cgggcgggag ataagaaact ggtggtcttg gccgccgccc gtctggggac gacgcgtctg attgacaatg tggaagtcgg cctgccttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for NegoA.00097.a.B1.PS37929
| PURIFICATION DATe: | 5/12/2016 |
| CONCENTRATION: | 41.5mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXXXHTIXXL RTWRENIGKV AFVPTMGNLH EGHLALVREA RKRADNVVVS IFVNRLQXXQ GEDFDKYPRT LQQDADKLAA EGVAVVFAPD EKELYPNVEQ RYNVEPPHLQ NELCGKFRPG HFRGVATVVS KLFNIVLPDV ACFGKKDYQQ LAVIKGLTED LNFDIEIVPV DTGRAADGLA LSSRNRYLSV GERAEAPRLY RELQAVAESL KQAGLDYAGL ERQAADHLTA AGWLVDYVEI RRADTLEMAR AGDKKLVVLA AARLGTTRLX XXVEVGLP
Validated NT Sequence
tcttactcac ttaaggcagg ccgacttcca catnnncnnt cagacgcgtc gtccccagac gggcggcggc caagaccacc agtttcttat ctcccgcccg cgccatttcg agcgtatcgg cgcggcggat ttccacataa tcgaccagcc agcccgcagc ggtcaaatgg tcggcagcct gccgctccaa acccgcataa tccaaaccgg cctgcttcag cgattcggca acagcctgca gctcgcggta caggcgcggt gcttcggcgc gttccccaac gctcaagtac cggttgcggc tggagagtgc caaaccgtcc gccgcccgcc ccgtatcgac ggggacaatt tcgatatcga aattcaaatc ctcggtcaaa cctttaatca cggcaagctg ctggtaatct tttttgccga aacaggcaac atccggcaaa acgatattga acagcttgga caccaccgtc gccacgccgc gaaaatggcc ggggcggaat ttgccgcaca attcgttttg caaatggggc ggttcgacgt tgtaacgctg ttccacgttc ggatagagtt ctttctcatc gggcgcgaaa acaacggcaa cgccttcggc ggcaagtttg tccgcatcct gttgcaaagt acgcggatat ttgtcgaaat cctcgccctg acnnaattgc aagcggttga cgaatatgct gacaacaacg ttgtcggcgc gttttctggc ttcgcgaaca agggcaagat gcccttcatg cagattgccc atggtcggca caaatgccac ctttcctata ttttcacgcc acgtgcgcag ttnnngaatg gtatgtnnna ntnncatatg gtggtggtgg tggtgannnn nggta
Expressed Protein Sequence
MAHHHHHHMQ IIHTIRELRT WRENTGKVAF VPTMGNLHEG HLALVREARK RADNVVVSIF VNRLQFGQGE DFDKYPRTLQ QDADKLAAEG VAVVFAPDEK ELYPNVEQRY NVEPPHLQNE LCGKFRPGHF RGVATVVSKL FNIVLPDVAC FGKKDYQQLA VIKGLTEDLN FDIEIVPVDT GRAADGLALS SRNRYLSVGE RAEAPRLYRE LQAVAESLKQ GGLDYAGLER QAADHLTAAG WLVDYVEIRR ADTLEMARAG DKKLVVLAAA RLGTTRLIDN VEVGLP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA TCATACATAC CATTCGAGAA CTGCGCACGT GGCGTGAAAA TACGGGAAAG GTGGCATTTG TGCCGACCAT GGGCAATCTG CATGAAGGGC ATCTTGCCCT TGTTCGCGAA GCCAGAAAAC GCGCCGACAA CGTTGTTGTC AGCATATTCG TCAACCGCTT GCAATTCGGT CAGGGCGAGG ATTTCGACAA ATATCCGCGT ACTTTGCAAC AGGATGCGGA CAAACTTGCC GCCGAAGGCG TTGCCGTTGT TTTCGCGCCC GATGAGAAAG AACTCTATCC GAACGTAGAA CAGCGTTACA ACGTCGAACC GCCCCATTTG CAAAACGAAT TGTGCGGCAA ATTCCGCCCC GGCCATTTTC GCGGTGTGGC GACGGTGGTG TCCAAGCTGT TCAATATCGT TTTGCCGGAT GTTGCCTGTT TCGGCAAAAA AGATTACCAG CAGCTTGCCG TGATTAAAGG TTTGACCGAG GATTTGAATT TCGATATCGA AATTGTCCCC GTCGATACGG GGCGGGCGGC GGACGGTTTG GCACTCTCCA GCCGCAACCG GTACTTGAGC GTTGGGGAAC GCGCCGAAGC ACCGCGCCTG TACCGCGAGC TGCAGGCTGT TGCCGAATCG CTGAAGCAGG GCGGTTTGGA TTATGCGGGT TTGGAGCGGC AGGCTGCCGA CCATTTGACC GCTGCGGGCT GGCTGGTCGA TTATGTGGAA ATCCGCCGCG CCGATACGCT CGAAATGGCG CGGGCGGGAG ATAAGAAACT GGTGGTCTTG GCCGCCGCCC GTCTGGGGAC GACGCGTCTG ATTGACAATG TGGAAGTCGG CCTGCCTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT