NegoA.00085.a

ADP-L-glycero-D-manno-heptose-6-epimerase

CENTER ID:	NegoA.00085.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00085.a.B1.GE39945	full length		1	334

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00085.a.B1.PS37956	full length		1	334

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.610
UniProt:	B4RKA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTIIVTGAAG FIGSNIVKAL NQRGITDIVA VDNLTKGEKF KNLAECEIAH YLDKHEFIRQ VREHILPYQN IEAVFHQGAC SDTMNHDGLY MMENNYQYTL DLLDWCQDER IPFLYASSAA VYGKGEIFRE ERELEKPLNV YGYSKFLFDQ VLRRRMKEGL TAQVVGFRYF NVYGQHEQHK GRMASVAFHH FHQYREHGYV NLFGSNDGYG NGEQTRDFVS VEDVAKINLY FFDHPELSGI YNLGTGRSQQ FNELAAATVN ACRAAEGKSE LSLKELVEEE LIRYIPFPDA LKGKYQGFTQ ADITKLREAG YKEEFFDVKA GVNRYVKWML ENLA

NT Sequence

ATGACCATCA TCGTAACAGG CGCGGCCGGC TTTATCGGCA GCAACATCGT CAAAGCCCTC AACCAACGCG GCATTACCGA CATCGTCGCC GTCGATAATC TGACAAAAGG CGAAAAATTC AAAAACCTTG CCGAGTGCGA AATCGCCCAC TACCTCGACA AACACGAATT TATCCGCCAA GTGAGGGAAC ACATTTTACC TTATCAAAAC ATCGAAGCCG TTTTCCATCA AGGCGCGTGT TCCGATACGA TGAACCACGA CGGTTTGTAT ATGATGGAAA ACAACTACCA GTACACGCTG GATTTGTTGG ACTGGTGTCA GGACGAACGC ATCCCCTTCC TTTATGCCTC CAGTGCGGCG GTTTACGGCA AAGGCGAAAT CTTCCGCGAA GAGCGCGAAC TCGAAAAACC GCTTAATGTG TACGGCTACT CCAAATTCCT GTTTGACCAA GTATTGCGCC GCCGCATGAA AGAAGGTCTC ACCGCCCAAG TCGTCGGCTT CCGCTACTTC AACGTTTACG GACAACACGA ACAACACAAA GGCCGCATGG CATCCGTCGC CTTCCACCAT TTCCACCAAT ACCGCGAACA CGGTTACGTC AACCTGTTCG GCAGCAACGA CGGCTACGGC AACGGCGAAC AAACCCGCGA CTTCGTCAGC GTCGAAGACG TCGCCAAAAT CAACCTCTAC TTCTTCGACC ATCCCGAACT TTCCGGCATC TACAACCTCG GCACCGGCCG CAGCCAACAG TTCAACGAAC TCGCCGCCGC CACCGTCAAC GCCTGCCGCG CCGCCGAAGG CAAATCTGAA TTGAGCTTGA AAGAGTTGGT AGAAGAAGAA CTTATCCGCT ATATCCCCTT TCCCGACGCG CTCAAAGGCA AATACCAGGG CTTCACCCAA GCCGACATCA CCAAATTGCG CGAAGCCGGA TATAAGGAAG AATTTTTCGA TGTCAAAGCA GGTGTCAACC GCTACGTCAA ATGGATGCTG GAAAATTTGG CTTAA

Details for NegoA.00085.a.B1.GE39945

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT IIVTGAAGFI GSNIVKALNQ RGITDIVAVD NLTKGEKFKN LAECEIAHYL DKHEFIRQVR EHILPYQNIE AVFHQGACSD TMNHDGLYMM ENNYQYTLDL LDWCQDERIP FLYASSAAVY GKGEIFREER ELEKPLNVYG YSKFLFDQVL RRRMKEGLTA QVVGFRYFNV YGQHEQHKGR MASVAFHHFH QYREHGYVNL FGSNDGYGNG EQTRDFVSVE DVAKINLYFF DHPELSGIYN LGTGRSQQFN ELAAAAVNAC RAAEGKSELS LKELVEEELI XXIPFPDALK GKYQGFTQAD ITKLREAGYK EEFFDVKAGV NRXXXXDAGK FG

Validated NT Sequence

aagccaaatt ttccagcatc nntttnnnnn nngcggttga cacctgcttt gacatcgaaa aattcttcct tatatccggc ttcgcgcaat ttggtgatgt cggcttgggt gaagccctgg tatttgcctt tgagcgcgtc gggaaagggg atatnnngga taagttcttc ttctaccaac tctttcaagc tcaattcaga tttgccttcg gcggcgcggc aggcgttgac ggcggcggcg gcgagttcgt tgaactgttg gctgcggccg gtgccgaggt tgtagatgcc ggaaagttcg ggatggtcga agaagtagag gttgattttg gcgacgtctt cgacgctgac gaagtcgcgg gtttgttcgc cgttgccgta gccgtcgttg ctgccgaaca ggttgacgta accgtgttcg cggtattggt ggaaatggtg gaaggcgacg gatgccatgc ggcctttgtg ttgttcgtgt tgtccgtaaa cgttgaagta gcggaagccg acgacttggg cggtgagacc ttctttcatg cggcggcgca atacttggtc aaacaggaat ttggagtagc cgtacacatt aagcggtttt tcgagttcgc gctcttcgcg gaagatttcg cctttgccgt aaaccgccgc actggaggca taaaggaagg ggatgcgttc gtcctgacac cagtccaaca aatccagcgt gtactggtag ttgttttcca tcatatacaa accgtcgtgg ttcatcgtat cggaacacgc gccttgatgg aaaacggctt cgatgttttg ataaggtaaa atgtgttccc tcacttggcg gataaattcg tgtttgtcga gataatgggc gatttcgcac tcggcaaggt ttttgaattt ttcgcctttt gtcagattat cgacggcgac gatgtcggta atgccgcgtt ggttgagggc tttgacgatg ttgctgccga taaagccggc cgcgcctgtt acgatgatgg tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMT IIVTGAAGFI GSNIVKALNQ RGITDIVAVD NLTKGEKFKN LAECEIAHYL DKHEFIRQVR EHILPYQNIE AVFHQGACSD TMNHDGLYMM ENNYQYTLDL LDWCQDERIP FLYASSAAVY GKGEIFREER ELEKPLNVYG YSKFLFDQVL RRRMKEGLTA QVVGFRYFNV YGQHEQHKGR MASVAFHHFH QYREHGYVNL FGSNDGYGNG EQTRDFVSVE DVAKINLYFF DHPELSGIYN LGTGRSQQFN ELAAATVNAC RAAEGKSELS LKELVEEELI RYIPFPDALK GKYQGFTQAD ITKLREAGYK EEFFDVKAGV NRYVKWMLEN LA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca tcatcgtaac aggcgcggcc ggctttatcg gcagcaacat cgtcaaagcc ctcaaccaac gcggcattac cgacatcgtc gccgtcgata atctgacaaa aggcgaaaaa ttcaaaaacc ttgccgagtg cgaaatcgcc cactacctcg acaaacacga atttatccgc caagtgaggg aacacatttt accttatcaa aacatcgaag ccgttttcca tcaaggcgcg tgttccgata cgatgaacca cgacggtttg tatatgatgg aaaacaacta ccagtacacg ctggatttgt tggactggtg tcaggacgaa cgcatcccct tcctttatgc ctccagtgcg gcggtttacg gcaaaggcga aatcttccgc gaagagcgcg aactcgaaaa accgcttaat gtgtacggct actccaaatt cctgtttgac caagtattgc gccgccgcat gaaagaaggt ctcaccgccc aagtcgtcgg cttccgctac ttcaacgttt acggacaaca cgaacaacac aaaggccgca tggcatccgt cgccttccac catttccacc aataccgcga acacggttac gtcaacctgt tcggcagcaa cgacggctac ggcaacggcg aacaaacccg cgacttcgtc agcgtcgaag acgtcgccaa aatcaacctc tacttcttcg accatcccga actttccggc atctacaacc tcggcaccgg ccgcagccaa cagttcaacg aactcgccgc cgccaccgtc aacgcctgcc gcgccgccga aggcaaatct gaattgagct tgaaagagtt ggtagaagaa gaacttatcc gctatatccc ctttcccgac gcgctcaaag gcaaatacca gggcttcacc caagccgaca tcaccaaatt gcgcgaagcc ggatataagg aagaattttt cgatgtcaaa gcaggtgtca accgctacgt caaatggatg ctggaaaatt tggcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for NegoA.00085.a.B1.PS37956

PURIFICATION DATe:	6/29/2016
CONCENTRATION:	40.8mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMT IIVTGAAGFI GSNIVKALNQ RGITDIVAVD NLTKGEKFKN LAECEIAHYL DKHEFIRQVR EHILPYQNIE AVFHQGACSD TMNHDGLYMM ENNYQYTLDL LDWCQDERIP FLYASSAAVY GKGEIFREER ELEKPLNVYG YSKFLFDQVL RRRMKEGLTA QVVGFRYFNV YGQHEQHKGR MASVAFHHFH QYREHGYVNL FGSNDGYGNG EQTRDFVSVE DVAKINLYFF DHPELSGIYN LGTGRSQQFN ELAAATVNAC RAAEGKSELS LKELVEEELI RYIPFPDALK GKYQGFTQAD ITKLREAGYK EEFFDVKAGV NRYVKWMLEN LA

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCA TCATCGTAAC AGGCGCGGCC GGCTTTATCG GCAGCAACAT CGTCAAAGCC CTCAACCAAC GCGGCATTAC CGACATCGTC GCCGTCGATA ATCTGACAAA AGGCGAAAAA TTCAAAAACC TTGCCGAGTG CGAAATCGCC CACTACCTCG ACAAACACGA ATTTATCCGC CAAGTGAGGG AACACATTTT ACCTTATCAA AACATCGAAG CCGTTTTCCA TCAAGGCGCG TGTTCCGATA CGATGAACCA CGACGGTTTG TATATGATGG AAAACAACTA CCAGTACACG CTGGATTTGT TGGACTGGTG TCAGGACGAA CGCATCCCCT TCCTTTATGC CTCCAGTGCG GCGGTTTACG GCAAAGGCGA AATCTTCCGC GAAGAGCGCG AACTCGAAAA ACCGCTTAAT GTGTACGGCT ACTCCAAATT CCTGTTTGAC CAAGTATTGC GCCGCCGCAT GAAAGAAGGT CTCACCGCCC AAGTCGTCGG CTTCCGCTAC TTCAACGTTT ACGGACAACA CGAACAACAC AAAGGCCGCA TGGCATCCGT CGCCTTCCAC CATTTCCACC AATACCGCGA ACACGGTTAC GTCAACCTGT TCGGCAGCAA CGACGGCTAC GGCAACGGCG AACAAACCCG CGACTTCGTC AGCGTCGAAG ACGTCGCCAA AATCAACCTC TACTTCTTCG ACCATCCCGA ACTTTCCGGC ATCTACAACC TCGGCACCGG CCGCAGCCAA CAGTTCAACG AACTCGCCGC CGCCACCGTC AACGCCTGCC GCGCCGCCGA AGGCAAATCT GAATTGAGCT TGAAAGAGTT GGTAGAAGAA GAACTTATCC GCTATATCCC CTTTCCCGAC GCGCTCAAAG GCAAATACCA GGGCTTCACC CAAGCCGACA TCACCAAATT GCGCGAAGCC GGATATAAGG AAGAATTTTT CGATGTCAAA GCAGGTGTCA ACCGCTACGT CAAATGGATG CTGGAAAATT TGGCTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT