NegoA.00084.a

enolase

CENTER ID:	NegoA.00084.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00084.a.B1.GE39944	full length		1	428

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00084.a.B1.PW37904	full length		1	428

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_85028

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.1389
UniProt:	B4RMD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAIVDIFAR EILDSRGNPT VECDVLLESG VMGRAAVPSG ASTGQKEALE LRDGDKSRYS GKGVLKAVEH VNNQIAQALI GIDANEQSYI DQIMIELDGT ENKGNLGANA TLAVSMAVAR AAAEDSGLPL YRYLGGAGPM SLPVPMMNVI NGGEHANNSL NIQEFMIMPV GAKSFREALR CGAEIFHALK KLCDSKGFPT TVGDEGGFAP NLNSHKEALQ LMVEAAEVAG YKAGEDVLFA LDCASSEFYK DGKYHLEAEG RSYTNAEFAE YLEGLVNEFP IISIEDGMDE NDWEGWKLLT EKLGKKVQLV GDDLFVTNPK ILAEGIEKGV ANALLVKVNQ IGTLSETLKA VDLAKCNRYA SVMSHRSGET EDSTIADLAV ATNCMQIKTG SLSRSDRMAK YNQLLRIEEE LAEAAYYPGK AAFYQLGK

NT Sequence

ATGAGCGCAA TCGTTGATAT TTTCGCCCGC GAAATTTTGG ACTCGCGCGG CAACCCCACA GTCGAGTGTG ATGTATTGCT CGAATCCGGC GTAATGGGAC GTGCGGCCGT ACCGAGCGGC GCATCCACCG GTCAGAAAGA AGCTTTGGAA CTTCGCGACG GCGACAAATC CCGCTATTCC GGCAAAGGCG TATTGAAGGC GGTCGAACAC GTCAACAACC AAATCGCCCA AGCCCTCATC GGTATCGATG CCAACGAGCA ATCTTATATC GACCAAATCA TGATCGAATT GGACGGTACT GAAAACAAAG GCAATTTGGG TGCGAATGCG ACTTTGGCGG TCTCTATGGC GGTTGCACGC GCCGCTGCCG AAGACTCAGG CCTGCCGCTT TACCGCTACT TGGGGGGCGC AGGTCCGATG TCCCTGCCCG TACCGATGAT GAACGTCATC AACGGCGGCG AACACGCCAA CAACAGCCTG AACATCCAAG AGTTTATGAT TATGCCCGTC GGCGCAAAAT CTTTCCGCGA AGCGTTGCGC TGCGGTGCGG AAATTTTCCA CGCCTTGAAA AAACTGTGCG ACAGTAAAGG CTTCCCGACC ACAGTCGGCG ACGAAGGCGG TTTCGCCCCC AACCTGAACA GCCACAAAGA AGCCCTGCAA CTGATGGTCG AAGCGGCCGA AGTCGCCGGC TACAAGGCGG GCGAAGACGT ATTATTCGCA TTGGACTGCG CGTCCAGCGA GTTCTACAAA GACGGCAAAT ACCACTTGGA AGCCGAAGGC CGCTCCTACA CCAACGCGGA ATTTGCCGAA TACTTGGAAG GCTTGGTTAA CGAATTCCCG ATTATTTCCA TTGAAGACGG GATGGACGAA AACGACTGGG AAGGCTGGAA ACTGCTGACC GAAAAATTGG GCAAAAAAGT TCAATTGGTC GGCGACGACT TGTTCGTAAC CAATCCGAAA ATTCTTGCCG AAGGCATCGA AAAAGGCGTA GCAAACGCAT TGCTGGTCAA AGTCAACCAA ATCGGTACTT TAAGCGAAAC CCTGAAAGCC GTCGATCTGG CAAAATGCAA CCGCTACGCC AGCGTGATGA GCCACCGCTC CGGCGAAACC GAAGACAGTA CCATTGCCGA CTTGGCAGTC GCCACCAACT GTATGCAGAT TAAAACCGGT TCTTTGAGCC GTTCCGACCG CATGGCGAAA TACAACCAAC TGCTGCGTAT CGAGGAAGAA TTGGCGGAAG CCGCCTACTA CCCCGGCAAA GCCGCATTCT ACCAACTGGG CAAATAA

Details for NegoA.00084.a.B1.GE39944

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	47kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS AIVDIFAREI LDSRGNPTVE CDVLLESGVM GRAAVPSGAS TGQKEALELR DGDKSRYSGK GVLKAVEHVN NQIAQALIGI DANEQSYIDQ IMIELDGTEN KGNLGANATL AVSMAVARAA AEDSGLPLYR YLGGAGPMSL PVPMMNVING GEHANNSLNI QEFMIMPVGA KSFREALRCG AEIFHALKKL CDSKGFPTTV GDEGGFAPNL NSHKEALQLM VEAAEAAGYK AGEXXLFALD CASSEFYKDG KXHLEAEGRS YTNAEFAEYL EGLXNEFPII SIEDGMDEND WEGWKLLTEK LGKKVQLVGD DLFVTNPKIL AEGIEKGVAN ALLVKVNQIG TLSETLKAVD LAKCNRYASV MSHRSGETED STIADLAVAT NCMQIKTGSL SRSDRMAKYN QLLRIEEELA EAAYYPG

Validated NT Sequence

ttgccggggt agtaggcggc ttccgccaat tcttcctcga tacgcagcag ttggttgtat ttcgccatgc ggtcggaacg gctcaaagaa ccggttttaa tctgcataca gttggtggcg actgccaagt cggcaatggt actgtcttcg gtttcgccgg agcggtggct catcacgctg gcgtagcggt tgcattttgc cagatcgacg gctttcaggg tttcgcttaa agtaccgatt tggttgactt tgaccagcaa tgcgtttgct acgccttttt cgatgccttc ggcaagaatt ttcggattgg ttacgaacaa gtcgtcgccg accaattgaa cttttttgcc caatttttcg gtcagcagtt tccagccttc ccagtcgttt tcgtccatcc cgtcttcaat ggaaataatc gggaattcgt tnnncaagcc ttccaagtat tcggcaaatt ccgcgttggt gtaggagcgg ccttcggctt ccaagtgnna tttgccgtct ttgtagaact cgctggacgc gcagtccaat gcgaataatn nnnnttcgcc cgccttgtag ccggcggctt cggccgcttc gaccatcagt tgcagggctt ctttgtggct gttcaggttg ggggcgaaac cgccttcgtc gccgactgtg gtcgggaagc ctttactgtc gcacagtttt ttcaaggcgt ggaaaatttc cgcaccgcag cgcaacgctt cgcggaaaga ttttgcgccg acgggcataa tcataaactc ttggatgttc aggctgttgt tggcgtgttc gccgccgttg atgacgttca tcatcggtac gggcagggac atcggacctg cgccccccaa gtagcggtaa agcggcaggc ctgagtcttc ggcagcggcg cgtgcaaccg ccatagagac cgccaaagtc gcattcgcac ccaaattgcc tttgttttca gtaccgtcca attcgatcat gatttggtcg atataagatt gctcgttggc atcgataccg atgagggctt gggcgatttg gttgttgacg tgttcgacgg ccttcaatac gcctttgccg gaatagcggg atttgtcgcc gtcgcgaagt tccaaagctt ctttctgacc ggtggatgcg ccgctcggta cggccgcacg tcccattacg ccggattcga gcaatacatc acactcgact gtggggttgc cgcgtgagtc caaaatttcg cgggcgaaaa tatcaacgat tgcgctcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMS AIVDIFAREI LDSRGNPTVE CDVLLESGVM GRAAVPSGAS TGQKEALELR DGDKSRYSGK GVLKAVEHVN NQIAQALIGI DANEQSYIDQ IMIELDGTEN KGNLGANATL AVSMAVARAA AEDSGLPLYR YLGGAGPMSL PVPMMNVING GEHANNSLNI QEFMIMPVGA KSFREALRCG AEIFHALKKL CDSKGFPTTV GDEGGFAPNL NSHKEALQLM VEAAEVAGYK AGEDVLFALD CASSEFYKDG KYHLEAEGRS YTNAEFAEYL EGLVNEFPII SIEDGMDEND WEGWKLLTEK LGKKVQLVGD DLFVTNPKIL AEGIEKGVAN ALLVKVNQIG TLSETLKAVD LAKCNRYASV MSHRSGETED STIADLAVAT NCMQIKTGSL SRSDRMAKYN QLLRIEEELA EAAYYPGKAA FYQLGK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg caatcgttga tattttcgcc cgcgaaattt tggactcgcg cggcaacccc acagtcgagt gtgatgtatt gctcgaatcc ggcgtaatgg gacgtgcggc cgtaccgagc ggcgcatcca ccggtcagaa agaagctttg gaacttcgcg acggcgacaa atcccgctat tccggcaaag gcgtattgaa ggcggtcgaa cacgtcaaca accaaatcgc ccaagccctc atcggtatcg atgccaacga gcaatcttat atcgaccaaa tcatgatcga attggacggt actgaaaaca aaggcaattt gggtgcgaat gcgactttgg cggtctctat ggcggttgca cgcgccgctg ccgaagactc aggcctgccg ctttaccgct acttgggggg cgcaggtccg atgtccctgc ccgtaccgat gatgaacgtc atcaacggcg gcgaacacgc caacaacagc ctgaacatcc aagagtttat gattatgccc gtcggcgcaa aatctttccg cgaagcgttg cgctgcggtg cggaaatttt ccacgccttg aaaaaactgt gcgacagtaa aggcttcccg accacagtcg gcgacgaagg cggtttcgcc cccaacctga acagccacaa agaagccctg caactgatgg tcgaagcggc cgaagtcgcc ggctacaagg cgggcgaaga cgtattattc gcattggact gcgcgtccag cgagttctac aaagacggca aataccactt ggaagccgaa ggccgctcct acaccaacgc ggaatttgcc gaatacttgg aaggcttggt taacgaattc ccgattattt ccattgaaga cgggatggac gaaaacgact gggaaggctg gaaactgctg accgaaaaat tgggcaaaaa agttcaattg gtcggcgacg acttgttcgt aaccaatccg aaaattcttg ccgaaggcat cgaaaaaggc gtagcaaacg cattgctggt caaagtcaac caaatcggta ctttaagcga aaccctgaaa gccgtcgatc tggcaaaatg caaccgctac gccagcgtga tgagccaccg ctccggcgaa accgaagaca gtaccattgc cgacttggca gtcgccacca actgtatgca gattaaaacc ggttctttga gccgttccga ccgcatggcg aaatacaacc aactgctgcg tatcgaggaa gaattggcgg aagccgccta ctaccccggc aaagccgcat tctaccaact gggcaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for NegoA.00084.a.B1.PW37904

PURIFICATION DATe:	5/4/2016
CONCENTRATION:	16mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMS AIVDIFAREI LDSRGNPTVE CDVLLESGVM GRAAVPSGAS TGQKEALELR DGDKSRYSGK GVLKAVEHVN NQIAQALIGI DANEQSYIDQ IMIELDGTEN KGNLGANATL AVSMAVARAA AEDSGLPLYR YLGGAGPMSL PVPMMNVING GEHANNSLNI QEFMIMPVGA KSFREALRCG AEIFHALKKL CDSKGFPTTV GDEGGFAPNL NSHKEALQLM VEAAEVAGYK AGEDVLFALD CASSEFYKDG KYHLEAEGRS YTNAEFAEYL EGLVNEFPII SIEDGMDEND WEGWKLLTEK LGKKVQLVGD DLFVTNPKIL AEGIEKGVAN ALLVKVNQIG TLSETLKAVD LAKCNRYASV MSHRSGETED STIADLAVAT NCMQIKTGSL SRSDRMAKYN QLLRIEEELA EAAYYPGKAA FYQLGK

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CAATCGTTGA TATTTTCGCC CGCGAAATTT TGGACTCGCG CGGCAACCCC ACAGTCGAGT GTGATGTATT GCTCGAATCC GGCGTAATGG GACGTGCGGC CGTACCGAGC GGCGCATCCA CCGGTCAGAA AGAAGCTTTG GAACTTCGCG ACGGCGACAA ATCCCGCTAT TCCGGCAAAG GCGTATTGAA GGCGGTCGAA CACGTCAACA ACCAAATCGC CCAAGCCCTC ATCGGTATCG ATGCCAACGA GCAATCTTAT ATCGACCAAA TCATGATCGA ATTGGACGGT ACTGAAAACA AAGGCAATTT GGGTGCGAAT GCGACTTTGG CGGTCTCTAT GGCGGTTGCA CGCGCCGCTG CCGAAGACTC AGGCCTGCCG CTTTACCGCT ACTTGGGGGG CGCAGGTCCG ATGTCCCTGC CCGTACCGAT GATGAACGTC ATCAACGGCG GCGAACACGC CAACAACAGC CTGAACATCC AAGAGTTTAT GATTATGCCC GTCGGCGCAA AATCTTTCCG CGAAGCGTTG CGCTGCGGTG CGGAAATTTT CCACGCCTTG AAAAAACTGT GCGACAGTAA AGGCTTCCCG ACCACAGTCG GCGACGAAGG CGGTTTCGCC CCCAACCTGA ACAGCCACAA AGAAGCCCTG CAACTGATGG TCGAAGCGGC CGAAGTCGCC GGCTACAAGG CGGGCGAAGA CGTATTATTC GCATTGGACT GCGCGTCCAG CGAGTTCTAC AAAGACGGCA AATACCACTT GGAAGCCGAA GGCCGCTCCT ACACCAACGC GGAATTTGCC GAATACTTGG AAGGCTTGGT TAACGAATTC CCGATTATTT CCATTGAAGA CGGGATGGAC GAAAACGACT GGGAAGGCTG GAAACTGCTG ACCGAAAAAT TGGGCAAAAA AGTTCAATTG GTCGGCGACG ACTTGTTCGT AACCAATCCG AAAATTCTTG CCGAAGGCAT CGAAAAAGGC GTAGCAAACG CATTGCTGGT CAAAGTCAAC CAAATCGGTA CTTTAAGCGA AACCCTGAAA GCCGTCGATC TGGCAAAATG CAACCGCTAC GCCAGCGTGA TGAGCCACCG CTCCGGCGAA ACCGAAGACA GTACCATTGC CGACTTGGCA GTCGCCACCA ACTGTATGCA GATTAAAACC GGTTCTTTGA GCCGTTCCGA CCGCATGGCG AAATACAACC AACTGCTGCG TATCGAGGAA GAATTGGCGG AAGCCGCCTA CTACCCCGGC AAAGCCGCAT TCTACCAACT GGGCAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT