SSGCID: Target Page

NegoA.00067.a

serine O-acetyltransferase

CENTER ID:	NegoA.00067.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00067.a.B1.GE39942	full length		1	272

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.1788
UniProt:	B4RNG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKNHLNTTG FDLWHTIREE TAAAAAAEPM LASFLHQTVL RHESLGSVLA YHLSSKLGSP IMDVRALFEI YQQALGSDTQ ISKCVEADLK AIYERDPACD EYSLPLLYFK GFHAIQAHRI NHRLYLDGRK TLAYFLQNRM SEVFGVDIHP AARLGYGLML DHATGFVAGE TAVLGNNISI LHGVTLGGSG KEGGDRHPKI GDGVMIGANA SILGNIRIGS NAKIGAGSVV VSDVPPSITV VGVPAKPVAR SLKTPSADMD QNIQFAEIDF MI

NT Sequence

ATGAAAAAAA ACCATTTGAA CACAACCGGT TTCGACCTCT GGCACACCAT CCGCGAAGAA ACCGCGGCCG CTGCCGCCGC CGAACCGATG CTGGCAAGTT TTTTGCACCA AACCGTGTTG CGCCACGAGT CCCTCGGCTC CGTCCTTGCC TACCACCTTT CCAGCAAACT CGGCAGCCCG ATTATGGACG TGCGCGCGCT GTTTGAAATT TACCAGCAGG CATTGGGCAG CGACACCCAA ATCAGCAAAT GTGTCGAAGC AGACTTAAAA GCCATCTACG AACGCGATCC CGCCTGCGAC GAATATTCGC TGCCGCTTTT ATATTTCAAA GGCTTCCACG CGATTCAGGC ACACCGCATC AACCACCGGC TGTATCTCGA CGGACGCAAA ACGCTGGCGT ATTTCCTACA AAACCGTATG TCCGAAGTAT TCGGCGTGGA CATCCATCCC GCCGCCCGTT TAGGATACGG GCTGATGCTC GACCACGCCA CCGGCTTTGT TGCCGGAGAA ACCGCCGTGT TGGGCAACAA TATTTCGATT TTGCACGGCG TAACGCTCGG CGGTTCGGGC AAAGAAGGCG GCGACCGCCA CCCCAAAATC GGCGACGGCG TGATGATCGG CGCAAACGCC TCGATATTGG GCAATATCCG CATCGGCAGC AATGCCAAAA TCGGCGCGGG CAGCGTCGTG GTTTCAGACG TGCCGCCGTC CATCACGGTT GTCGGCGTAC CCGCCAAACC CGTGGCGCGA TCGCTCAAAA CCCCGTCGGC GGATATGGAT CAAAATATCC AGTTTGCCGA AATCGACTTT ATGATTTGA

Details for NegoA.00067.a.B1.GE39942

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK KNHLNTTGFD LWHTIREETA AAAAAEPMLA SFLHQTVLRH ESLGSVLAYH LSSKLGSPIM DVRALFEIYQ QALGSDTQIS KCVEADLKAI YERDPACDEY SLPLLYFKGF HAIQAHRINH RLYLDGRKTL AYFLQNRMSE VFGVDIHPAA RLGYGLMLDH ATGFVAGETA VLGNNISILH GVTLGGSGKE GGDRHPKIGD GVMIGANASI LGNIRIGSNA KIGAGSVVVS DVPPSITVVG VPAKPVARSL KTPSADMDQN IXXAEIDFMI

Validated NT Sequence

atcttactca cttaaatcat aaagtcgatt tcggcanant ggatattttg atccatatcc gccgacgggg ttttgagcga tcgcgccacg ggtttggcgg gtacgccgac aaccgtgatg gacggcggca cgtctgaaac cacgacgctg cccgcgccga ttttggcatt gctgccgatg cggatattgc ccaatatcga ggcgtttgcg ccgatcatca cgccgtcgcc gattttgggg tggcggtcgc cgccttcttt gcccgaaccg ccgagcgtta cgccgtgcaa aatcgaaata ttgttgccca acacggcggt ttctccggca acaaagccgg tggcgtggtc gagcatcagc ccgtatccta aacgggcggc gggatggatg tccacgccga atacttcgga catacggttt tgtaggaaat acgccagcgt tttgcgtccg tcgagataca gccggtggtt gatgcggtgt gcctgaatcg cgtggaagcc tttgaaatat aaaagcggca gcgaatattc gtcgcaggcg ggatcgcgtt cgtagatggc ttttaagtct gcttcgacac atttgctgat ttgggtgtcg ctgcccaatg cctgctggta aatttcaaac agcgcgcgca cgtccataat cgggctgccg agtttgctgg aaaggtggta ggcaaggacg gagccgaggg actcgtggcg caacacggtt tggtgcaaaa aacttgccag catcggttcg gcggcggcag cggccgcggt ttcttcgcgg atggtgtgcc agaggtcgaa accggttgtg ttcaaatggt tttttttcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaaaccattt gaacacaacc ggtttcgacc tctggcacac catccgcgaa gaaaccgcgg ccgctgccgc cgccgaaccg atgctggcaa gttttttgca ccaaaccgtg ttgcgccacg agtccctcgg ctccgtcctt gcctaccacc tttccagcaa actcggcagc ccgattatgg acgtgcgcgc gctgtttgaa atttaccagc aggcattggg cagcgacacc caaatcagca aatgtgtcga agcagactta aaagccatct acgaacgcga tcccgcctgc gacgaatatt cgctgccgct tttatatttc aaaggcttcc acgcgattca ggcacaccgc atcaaccacc ggctgtatct cgacggacgc aaaacgctgg cgtatttcct acaaaaccgt atgtccgaag tattcggcgt ggacatccat cccgccgccc gtttaggata cgggctgatg ctcgaccacg ccaccggctt tgttgccgga gaaaccgccg tgttgggcaa caatatttcg attttgcacg gcgtaacgct cggcggttcg ggcaaagaag gcggcgaccg ccaccccaaa atcggcgacg gcgtgatgat cggcgcaaac gcctcgatat tgggcaatat ccgcatcggc agcaatgcca aaatcggcgc gggcagcgtc gtggtttcag acgtgccgcc gtccatcacg gttgtcggcg tacccgccaa acccgtggcg cgatcgctca aaaccccgtc ggcggatatg gatcaaaata tccagtttgc cgaaatcgac tttatgattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat