NegoA.00040.a
S-adenosylmethionine synthase
| CENTER ID: | NegoA.00040.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00040.a.B1.GE39939 | full length | 1 | 389 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.00040.a.B1.PW37902 | full length | 1 | 389 |
Structures
5T8S
DEPOSITED: 9/8/2016
DETERMINATION: XRay
CLONE: NegoA.00040.a.B1.GE39939
PROTEIN: NegoA.00040.a.B1.PW37902
5T8T
DEPOSITED: 9/8/2016
DETERMINATION: XRay
CLONE: NegoA.00040.a.B1.GE39939
PROTEIN: NegoA.00040.a.B1.PW37902
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.169 |
| UniProt: | B4RQ36 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEYLFTSES VSEGHPDKVA DQVSDAILDA ILAQDPKARV AAETLVNTGL CVLAGEITTT AQVDYIKVAR ETIKRIGYNS SELGFDANGC AVGVYYDQQS PDIAQGVNEG EGIDLNQGAG DQGLMFGYAC DETPTLMPFA IYYSHRLMQR QSELRKDGRL PWLRPDAKAQ LTVVYDSETG KVKRIDTVVL STQHDPAISH EELSKAVIEQ IIKPVLPPEL LTDETKYLIN PTGRFVIGGP QGDCGLTGRK IIVDTYGGAA PHGGGAFSGK DPSKVDRSAA YACRYVAKNI VAAGLATQCQ IQVSYAIGVA EPTSISIDTF GTGKISEEKL IALVCEHFDL RPKGIVQMLD LLRPIYGKSA AYGHFGREEP EFTWERTDKA ASLKAAAGL
NT Sequence
ATGAGCGAAT ATCTGTTTAC TTCCGAATCG GTTTCCGAAG GCCATCCGGA CAAAGTAGCC GACCAAGTAT CCGATGCGAT TTTGGATGCC ATCTTGGCGC AAGACCCCAA AGCGCGTGTC GCGGCGGAAA CTTTGGTCAA CACAGGCTTG TGCGTATTGG CGGGCGAAAT CACCACCACC GCCCAAGTGG ACTACATCAA AGTCGCACGC GAAACCATCA AACGCATCGG CTACAACTCC TCCGAGCTGG GCTTTGACGC CAACGGCTGC GCGGTCGGCG TGTACTACGA CCAACAATCC CCCGACATCG CCCAAGGCGT GAACGAAGGC GAAGGCATCG ACCTGAACCA AGGCGCGGGC GACCAAGGCT TGATGTTCGG CTACGCCTGC GACGAAACCC CGACCCTGAT GCCGTTTGCC ATCTATTACA GCCACCGCCT GATGCAGCGT CAAAGCGAAT TGCGCAAAGA CGGCCGCCTG CCTTGGCTGC GCCCTGACGC AAAAGCCCAA CTGACCGTGG TTTACGACAG CGAAACCGGC AAGGTGAAAC GCATTGATAC CGTCGTCCTG TCCACCCAGC ACGATCCTGC CATCAGCCAT GAAGAACTGA GTAAAGCCGT GATTGAGCAG ATTATCAAGC CCGTTTTGCC GCCCGAACTG CTGACCGACG AAACAAAATA CCTGATCAAC CCGACCGGCC GCTTCGTCAT CGGCGGCCCG CAAGGCGACT GCGGTTTGAC CGGCCGCAAA ATCATTGTCG ATACCTACGG CGGCGCGGCT CCGCACGGCG GCGGCGCATT CTCCGGCAAA GACCCGTCCA AAGTGGACCG TTCCGCCGCC TACGCCTGCC GTTATGTGGC GAAAAACATC GTCGCCGCAG GCTTGGCAAC CCAATGCCAA ATCCAAGTTT CCTACGCCAT CGGCGTTGCC GAACCGACTT CGATTTCCAT CGATACTTTC GGTACGGGTA AAATCAGCGA GGAAAAACTG ATTGCGCTGG TTTGCGAACA TTTCGACCTG CGTCCCAAAG GCATCGTCCA AATGCTCGAC CTCTTGCGCC CGATTTACGG AAAATCTGCC GCCTACGGAC ATTTCGGCCG CGAAGAACCC GAGTTTACTT GGGAGCGCAC CGACAAAGCG GCCTCATTGA AAGCGGCAGC GGGGCTGTAA
Details for NegoA.00040.a.B1.GE39939
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS EYLFTSESVS EGHPDKVADQ VSDAILDAIL AQDPKARVAA ETLVNTGLCV LAGEITTTAQ VDYIKVARET IKRIGYNSSE LGFDANGCAV GVYYDQQSPD IAQGVNEGEG IDLNQGAGDQ GLMFGYACDE TPTLMPFAIY YSHRLMQRQS ELRKDGRLPW LRPDAKAQLT VVYDSETGKV KRIDTVVLST QHDPAISQEE LSKAVIEQII KPVLPPELLT DETKYLINPT GRFVIGGPQG DCGLTGRKII VDTYGGAAPH GGGAFSGKDP SKVDRSAAYA CRYVAKNIVA AGLATQCQIQ VSYAIGVAEP TSISIDTFGT GKISEEKLIA LVCEHFDLRP KGIVQMLDLL RPIYGKSAAY GHFGREEPEF TWERTDKAA
Validated NT Sequence
tgaggccgct ttgtcggtgc gttcccaagt aaactcgggt tcttcgcggc cgaaatgccc gtaggcggca gattttccgt aaatcgggcg caagaggtcg agcatttgga cgatgccttt gggacgcagg tcgaaatgtt cgcaaaccag cgcaatcagt ttttcctcgc tgattttacc cgtaccgaaa gtatcgatgg aaatcgaagt cggttcggca acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaagcctgc ggcgacgatg tttttcgcca cataacggca ggcgtaagcg gcggaacggt ccactttgga cgggtctttg ccggagaatg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcgac aatgattttg cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg atcaggtatt ttgtttcgtc ggtcagcagt tcgggcggca aaacgggctt gataatctgc tcaatcacgg ctttactcag ttcttcctgg ctgatggcag gatcgtgctg ggtggacagg acgacggtat caatgcgttt caccttgccg gtttcgctgt cgtaaaccac ggtcagttgg gcttttgcgt cagggcgcag ccaaggcagg cggccgtctt tgcgcaattc gctttgacgc tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt ttcgtcgcag gcatagccga acatcaagcc ttggtcgccc gcgccttggt tcaggtcgat gccttcgcct tcgttcacgc cttgggcgat gtcgggggat tgttggtcgt agtacacgcc gaccgcgcag ccgttggcgt caaagcccag ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcccgccaa tacgcacaag cctgtgttga ccaaagtttc cgccgcgaca cgcgctttgg ggtcttgcgc caagatggca tccaaaatcg catcggatac ttggtcggct actttgtccg gatggccttc ggaaaccgat tcggaagtaa acagatattc gctcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS EYLFTSESVS EGHPDKVADQ VSDAILDAIL AQDPKARVAA ETLVNTGLCV LAGEITTTAQ VDYIKVARET IKRIGYNSSE LGFDANGCAV GVYYDQQSPD IAQGVNEGEG IDLNQGAGDQ GLMFGYACDE TPTLMPFAIY YSHRLMQRQS ELRKDGRLPW LRPDAKAQLT VVYDSETGKV KRIDTVVLST QHDPAISHEE LSKAVIEQII KPVLPPELLT DETKYLINPT GRFVIGGPQG DCGLTGRKII VDTYGGAAPH GGGAFSGKDP SKVDRSAAYA CRYVAKNIVA AGLATQCQIQ VSYAIGVAEP TSISIDTFGT GKISEEKLIA LVCEHFDLRP KGIVQMLDLL RPIYGKSAAY GHFGREEPEF TWERTDKAAS LKAAAGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg aatatctgtt tacttccgaa tcggtttccg aaggccatcc ggacaaagta gccgaccaag tatccgatgc gattttggat gccatcttgg cgcaagaccc caaagcgcgt gtcgcggcgg aaactttggt caacacaggc ttgtgcgtat tggcgggcga aatcaccacc accgcccaag tggactacat caaagtcgca cgcgaaacca tcaaacgcat cggctacaac tcctccgagc tgggctttga cgccaacggc tgcgcggtcg gcgtgtacta cgaccaacaa tcccccgaca tcgcccaagg cgtgaacgaa ggcgaaggca tcgacctgaa ccaaggcgcg ggcgaccaag gcttgatgtt cggctacgcc tgcgacgaaa ccccgaccct gatgccgttt gccatctatt acagccaccg cctgatgcag cgtcaaagcg aattgcgcaa agacggccgc ctgccttggc tgcgccctga cgcaaaagcc caactgaccg tggtttacga cagcgaaacc ggcaaggtga aacgcattga taccgtcgtc ctgtccaccc agcacgatcc tgccatcagc catgaagaac tgagtaaagc cgtgattgag cagattatca agcccgtttt gccgcccgaa ctgctgaccg acgaaacaaa atacctgatc aacccgaccg gccgcttcgt catcggcggc ccgcaaggcg actgcggttt gaccggccgc aaaatcattg tcgataccta cggcggcgcg gctccgcacg gcggcggcgc attctccggc aaagacccgt ccaaagtgga ccgttccgcc gcctacgcct gccgttatgt ggcgaaaaac atcgtcgccg caggcttggc aacccaatgc caaatccaag tttcctacgc catcggcgtt gccgaaccga cttcgatttc catcgatact ttcggtacgg gtaaaatcag cgaggaaaaa ctgattgcgc tggtttgcga acatttcgac ctgcgtccca aaggcatcgt ccaaatgctc gacctcttgc gcccgattta cggaaaatct gccgcctacg gacatttcgg ccgcgaagaa cccgagttta cttgggagcg caccgacaaa gcggcctcat tgaaagcggc agcggggctg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NegoA.00040.a.B1.PW37902
| PURIFICATION DATe: | 5/4/2016 |
| CONCENTRATION: | 31.5mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS EYLFTSESVS EGHPDKVADQ VSDAILDAIL AQDPKARVAA ETLVNTGLCV LAGEITTTAQ VDYIKVARET IKRIGYNSSE LGFDANGCAV GVYYDQQSPD IAQGVNEGEG IDLNQGAGDQ GLMFGYACDE TPTLMPFAIY YSHRLMQRQS ELRKDGRLPW LRPDAKAQLT VVYDSETGKV KRIDTVVLST QHDPAISQEE LSKAVIEQII KPVLPPELLT DETKYLINPT GRFVIGGPQG DCGLTGRKII VDTYGGAAPH GGGAFSGKDP SKVDRSAAYA CRYVAKNIVA AGLATQCQIQ VSYAIGVAEP TSISIDTFGT GKISEEKLIA LVCEHFDLRP KGIVQMLDLL RPIYGKSAAY GHFGREEPEF TWERTDKAA
Validated NT Sequence
tgaggccgct ttgtcggtgc gttcccaagt aaactcgggt tcttcgcggc cgaaatgccc gtaggcggca gattttccgt aaatcgggcg caagaggtcg agcatttgga cgatgccttt gggacgcagg tcgaaatgtt cgcaaaccag cgcaatcagt ttttcctcgc tgattttacc cgtaccgaaa gtatcgatgg aaatcgaagt cggttcggca acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaagcctgc ggcgacgatg tttttcgcca cataacggca ggcgtaagcg gcggaacggt ccactttgga cgggtctttg ccggagaatg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcgac aatgattttg cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg atcaggtatt ttgtttcgtc ggtcagcagt tcgggcggca aaacgggctt gataatctgc tcaatcacgg ctttactcag ttcttcctgg ctgatggcag gatcgtgctg ggtggacagg acgacggtat caatgcgttt caccttgccg gtttcgctgt cgtaaaccac ggtcagttgg gcttttgcgt cagggcgcag ccaaggcagg cggccgtctt tgcgcaattc gctttgacgc tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt ttcgtcgcag gcatagccga acatcaagcc ttggtcgccc gcgccttggt tcaggtcgat gccttcgcct tcgttcacgc cttgggcgat gtcgggggat tgttggtcgt agtacacgcc gaccgcgcag ccgttggcgt caaagcccag ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcccgccaa tacgcacaag cctgtgttga ccaaagtttc cgccgcgaca cgcgctttgg ggtcttgcgc caagatggca tccaaaatcg catcggatac ttggtcggct actttgtccg gatggccttc ggaaaccgat tcggaagtaa acagatattc gctcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS EYLFTSESVS EGHPDKVADQ VSDAILDAIL AQDPKARVAA ETLVNTGLCV LAGEITTTAQ VDYIKVARET IKRIGYNSSE LGFDANGCAV GVYYDQQSPD IAQGVNEGEG IDLNQGAGDQ GLMFGYACDE TPTLMPFAIY YSHRLMQRQS ELRKDGRLPW LRPDAKAQLT VVYDSETGKV KRIDTVVLST QHDPAISHEE LSKAVIEQII KPVLPPELLT DETKYLINPT GRFVIGGPQG DCGLTGRKII VDTYGGAAPH GGGAFSGKDP SKVDRSAAYA CRYVAKNIVA AGLATQCQIQ VSYAIGVAEP TSISIDTFGT GKISEEKLIA LVCEHFDLRP KGIVQMLDLL RPIYGKSAAY GHFGREEPEF TWERTDKAAS LKAAAGL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG AATATCTGTT TACTTCCGAA TCGGTTTCCG AAGGCCATCC GGACAAAGTA GCCGACCAAG TATCCGATGC GATTTTGGAT GCCATCTTGG CGCAAGACCC CAAAGCGCGT GTCGCGGCGG AAACTTTGGT CAACACAGGC TTGTGCGTAT TGGCGGGCGA AATCACCACC ACCGCCCAAG TGGACTACAT CAAAGTCGCA CGCGAAACCA TCAAACGCAT CGGCTACAAC TCCTCCGAGC TGGGCTTTGA CGCCAACGGC TGCGCGGTCG GCGTGTACTA CGACCAACAA TCCCCCGACA TCGCCCAAGG CGTGAACGAA GGCGAAGGCA TCGACCTGAA CCAAGGCGCG GGCGACCAAG GCTTGATGTT CGGCTACGCC TGCGACGAAA CCCCGACCCT GATGCCGTTT GCCATCTATT ACAGCCACCG CCTGATGCAG CGTCAAAGCG AATTGCGCAA AGACGGCCGC CTGCCTTGGC TGCGCCCTGA CGCAAAAGCC CAACTGACCG TGGTTTACGA CAGCGAAACC GGCAAGGTGA AACGCATTGA TACCGTCGTC CTGTCCACCC AGCACGATCC TGCCATCAGC CATGAAGAAC TGAGTAAAGC CGTGATTGAG CAGATTATCA AGCCCGTTTT GCCGCCCGAA CTGCTGACCG ACGAAACAAA ATACCTGATC AACCCGACCG GCCGCTTCGT CATCGGCGGC CCGCAAGGCG ACTGCGGTTT GACCGGCCGC AAAATCATTG TCGATACCTA CGGCGGCGCG GCTCCGCACG GCGGCGGCGC ATTCTCCGGC AAAGACCCGT CCAAAGTGGA CCGTTCCGCC GCCTACGCCT GCCGTTATGT GGCGAAAAAC ATCGTCGCCG CAGGCTTGGC AACCCAATGC CAAATCCAAG TTTCCTACGC CATCGGCGTT GCCGAACCGA CTTCGATTTC CATCGATACT TTCGGTACGG GTAAAATCAG CGAGGAAAAA CTGATTGCGC TGGTTTGCGA ACATTTCGAC CTGCGTCCCA AAGGCATCGT CCAAATGCTC GACCTCTTGC GCCCGATTTA CGGAAAATCT GCCGCCTACG GACATTTCGG CCGCGAAGAA CCCGAGTTTA CTTGGGAGCG CACCGACAAA GCGGCCTCAT TGAAAGCGGC AGCGGGGCTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT