NegoA.00030.a

ATP-dependent Clp protease ATP-binding subunit ClpX

CENTER ID: NegoA.00030.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.00030.a.B1.GE39938 full length 1 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.1356
UniProt: B4RMA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNENRTCSF CGKSKSHVKH LIEGENAFIC DECVANCLEI LYEGDNGGTP PENAGGEPEE SGKLPTPAEI VANLDDYVIG QGQAKKALAV AVYNHYKRLR HPKADGGVEL SKSNILLIGP TGSGKTLLAQ SLARKLDVPF VMADATTLTE AGYVGEDVEQ IITKLLGKCD FDVEKAQHGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA LLKLIEGTVA SVPPQGGRKH PNQEFINVDT ANILFICGGA FAGLEKVIRQ RTEKGGIGFG ASVHSKDENA GITKLFGIVE PEDLIKFGLI PELIGRLPVI ATLEELDEDA LINILTEPKN ALVKQYQALF GIENVGLEFE EGALRSIARQ AMERKTGARG LRSIVERCLL DTMYRLPDLQ GLKKVVVGKA VIEEGREPEL VFES
NT Sequence
ATGTCCAACG AAAACCGTAC CTGTTCCTTT TGCGGAAAAT CCAAATCACA CGTCAAACAT TTGATTGAGG GCGAAAACGC CTTTATCTGC GACGAATGCG TCGCAAACTG CCTCGAAATA TTGTACGAAG GCGACAACGG CGGCACGCCT CCGGAAAATG CCGGAGGGGA GCCGGAAGAA TCCGGCAAAC TGCCCACGCC CGCCGAAATC GTTGCCAACC TCGACGATTA TGTCATCGGG CAGGGGCAGG CGAAAAAGGC GCTGGCGGTT GCGGTTTACA ACCATTACAA ACGCCTGCGC CACCCGAAAG CCGACGGCGG TGTCGAATTG TCGAAATCCA ACATCCTGCT TATCGGCCCG ACCGGATCGG GTAAAACGCT GTTGGCGCAA TCTTTGGCAC GCAAACTGGA CGTGCCGTTC GTGATGGCGG ATGCGACCAC GCTGACCGAA GCCGGCTATG TCGGCGAAGA TGTCGAACAA ATCATTACCA AACTGTTGGG CAAATGCGAT TTCGATGTCG AAAAAGCCCA GCACGGCATT GTCTATATTG ACGAAATTGA CAAAATTTCG CGTAAAAGCG ACAACCCGTC CATCACGCGC GACGTGTCCG GCGAAGGCGT GCAGCAAGCC TTGCTGAAAC TGATTGAAGG TACGGTGGCA AGCGTTCCGC CCCAAGGCGG ACGCAAGCAT CCGAATCAGG AATTTATCAA CGTTGATACC GCCAACATCC TGTTTATCTG CGGCGGCGCG TTTGCAGGCT TGGAAAAAGT GATTCGCCAG CGCACCGAGA AAGGTGGTAT CGGTTTCGGC GCGTCCGTTC ACAGCAAGGA CGAAAATGCC GGCATTACCA AGCTGTTCGG CATCGTCGAA CCGGAAGATT TAATCAAATT CGGCCTGATT CCGGAATTAA TCGGACGTTT GCCCGTGATT GCAACTTTAG AAGAACTGGA TGAGGACGCG CTGATTAATA TTTTAACCGA GCCGAAAAAC GCTTTGGTCA AGCAGTATCA AGCCTTGTTC GGCATAGAAA ACGTCGGGTT GGAATTTGAA GAAGGCGCAT TGCGTTCCAT CGCGCGGCAG GCAATGGAAC GCAAAACCGG CGCGCGCGGC CTGCGTTCCA TCGTCGAACG CTGCCTGCTC GATACGATGT ACCGCCTGCC CGATTTGCAA GGCTTGAAAA AAGTGGTGGT CGGCAAGGCA GTCATCGAAG AGGGCAGGGA ACCGGAATTG GTGTTCGAGT CCTGA
Details for NegoA.00030.a.B1.GE39938
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS NENRTCSFCG KSKSHAKHLI EGKNAYICDE CVANCLEILY EGDNGGTPPE NAGGEPEESG KLPTPAEIVA NLDDYVIGQG QAKKALAVAV YNHYKRLRHP KADGGVELSK SNILLIGPTG SGKTLLAQSL ARKLDVPFVM ADATTLTEAG YVGEDVEQII TKLLGKCDFD VEKARHGIVY IDEIDKISRK SDNPSITRDV SGEGVQQALL KLIEGTVASV PPQGGRKHPN QEFINVDTAN ILFICGGAFA GLEKVIRQRT EKGGIGFGAS VHSKDENAGI TKLFGIVEPE DLIKFGLIPE LIGRLPVIAT LEELDEDALI NILTEPKNAL VKQYQALFGI ENVGLEFEEG ALRSIARQAM ERKTGARGLR SIVERCLLDT MYRLPDLQGL KKVVVGKAVI EEGR
Validated NT Sequence
tccctgccct cttcgatgac tgccttgccg accaccactt ttttcaagcc ttgcaaatcg ggcaggcggt acatcgtatc gagcaggcag cgttcgacga tggaacgcag gccgcgcgcg ccggttttgc gttccattgc ctgccgcgcg atggaacgca atgcgccttc ttcaaattcc aacccgacgt tttctatgcc gaacaaggct tgatactgct tgaccaaagc gtttttcggc tcggttaaaa tattaatcag cgcgtcctca tccagttctt ctaaagttgc aatcacgggc aaacgtccga ttaattccgg aatcaggccg aatttgatta aatcttccgg ttcgacgatg ccgaacagct tggtaatgcc ggcattttcg tccttgctgt gaacggacgc gccgaaaccg ataccacctt tctcggtgcg ctggcgaatc actttttcca agcctgcaaa cgcgccgccg cagataaaca ggatgttggc ggtatcaacg ttgataaatt cctgattcgg atgcttgcgt ccgccttggg gcggaacgct tgccaccgta ccttcaatca gtttcagcaa ggcttgctgc acgccttcgc cggacacgtc gcgcgtgatg gacgggttgt cgcttttacg cgaaattttg tcaatttcgt caatatagac aatgccgtgc cgggcttttt cgacatcgaa atcgcatttg cccaacagtt tggtaatgat ttgttcgaca tcttcgccga catagccggc ttcggtcagc gtggtcgcat ccgccatcac gaacggcacg tccagtttgc gtgccaaaga ttgcgccaac agcgttttac ccgatccggt cgggccgata agcaggatgt tggatttcga caattcgaca ccgccgtcgg ctttcgggtg gcgcaggcgt ttgtaatggt tgtaaaccgc aaccgccagc gcctttttcg cctgcccctg cccgatgaca taatcgtcga ggttggcaac gatttcggcg ggcgtgggca gtttgccgga ttcttccggc tcccctccgg cattttccgg aggcgtgccg ccgttgtcgc cttcgtacaa tatttcgagg cagtttgcga cgcattcgtc gcagatatag gcgtttttgc cctcaatcag gtgtttggcg tgtgatttgg attttccgca aaaggaacag gtacggtttt cgttggacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS NENRTCSFCG KSKSHVKHLI EGENAFICDE CVANCLEILY EGDNGGTPPE NAGGEPEESG KLPTPAEIVA NLDDYVIGQG QAKKALAVAV YNHYKRLRHP KADGGVELSK SNILLIGPTG SGKTLLAQSL ARKLDVPFVM ADATTLTEAG YVGEDVEQII TKLLGKCDFD VEKAQHGIVY IDEIDKISRK SDNPSITRDV SGEGVQQALL KLIEGTVASV PPQGGRKHPN QEFINVDTAN ILFICGGAFA GLEKVIRQRT EKGGIGFGAS VHSKDENAGI TKLFGIVEPE DLIKFGLIPE LIGRLPVIAT LEELDEDALI NILTEPKNAL VKQYQALFGI ENVGLEFEEG ALRSIARQAM ERKTGARGLR SIVERCLLDT MYRLPDLQGL KKVVVGKAVI EEGREPELVF ES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca acgaaaaccg tacctgttcc ttttgcggaa aatccaaatc acacgtcaaa catttgattg agggcgaaaa cgcctttatc tgcgacgaat gcgtcgcaaa ctgcctcgaa atattgtacg aaggcgacaa cggcggcacg cctccggaaa atgccggagg ggagccggaa gaatccggca aactgcccac gcccgccgaa atcgttgcca acctcgacga ttatgtcatc gggcaggggc aggcgaaaaa ggcgctggcg gttgcggttt acaaccatta caaacgcctg cgccacccga aagccgacgg cggtgtcgaa ttgtcgaaat ccaacatcct gcttatcggc ccgaccggat cgggtaaaac gctgttggcg caatctttgg cacgcaaact ggacgtgccg ttcgtgatgg cggatgcgac cacgctgacc gaagccggct atgtcggcga agatgtcgaa caaatcatta ccaaactgtt gggcaaatgc gatttcgatg tcgaaaaagc ccagcacggc attgtctata ttgacgaaat tgacaaaatt tcgcgtaaaa gcgacaaccc gtccatcacg cgcgacgtgt ccggcgaagg cgtgcagcaa gccttgctga aactgattga aggtacggtg gcaagcgttc cgccccaagg cggacgcaag catccgaatc aggaatttat caacgttgat accgccaaca tcctgtttat ctgcggcggc gcgtttgcag gcttggaaaa agtgattcgc cagcgcaccg agaaaggtgg tatcggtttc ggcgcgtccg ttcacagcaa ggacgaaaat gccggcatta ccaagctgtt cggcatcgtc gaaccggaag atttaatcaa attcggcctg attccggaat taatcggacg tttgcccgtg attgcaactt tagaagaact ggatgaggac gcgctgatta atattttaac cgagccgaaa aacgctttgg tcaagcagta tcaagccttg ttcggcatag aaaacgtcgg gttggaattt gaagaaggcg cattgcgttc catcgcgcgg caggcaatgg aacgcaaaac cggcgcgcgc ggcctgcgtt ccatcgtcga acgctgcctg ctcgatacga tgtaccgcct gcccgatttg caaggcttga aaaaagtggt ggtcggcaag gcagtcatcg aagagggcag ggaaccggaa ttggtgttcg agtcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat