SSGCID: Target Page

NegoA.00011.a

cell division protein ftsZ

CENTER ID:	NegoA.00011.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.00011.a.B1.GE39936	full length		1	392

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.1939
UniProt:	B4RQC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEFVYDVAES AVSPAVIKVI GLGGGGCNAI NNMVANNVRS VEFISANTDA QSLAKNHAAK RIQLGTNLTR GLGAGANPDI GRAAAQEDRE AIEEAIRGAN MLFITTGMGG GTGTGSAPVV AEIAKSLGIL TVAVVTRPFS YEGKRVHVAQ AGLEQLKEYV DSLIIIPNDK LMTALGEDVT MREAFRAADN VLRDAVAGIS EVVTCPSEII NLDFADVKTV MSNRGIAMMG SGYAQGIDRA RMATDQAISS PLLDDVTLDG ARGVLVNITT APGCLKMSEL SEVMKIVNQS AHPDLECKFG AAEDETMSED AIRITIIATG LKEKGAVDPT PAREVEAVAP SKQEQSHNVE GMIRTNRGIR TMNLTAADFD NQSVLDDFEI PAILRRQHNS DK

NT Sequence

ATGGAATTTG TTTACGACGT GGCAGAATCG GCAGTCAGCC CTGCGGTAAT TAAAGTAATC GGCTTGGGCG GCGGCGGTTG CAATGCAATC AATAACATGG TTGCCAACAA TGTGCGCAGT GTGGAGTTTA TCAGTGCCAA TACAGATGCG CAGTCTTTGG CAAAAAACCA TGCGGCGAAG AGAATCCAGT TGGGTACGAA TCTGACACGC GGTTTGGGCG CGGGTGCGAA TCCCGATATC GGCCGTGCGG CAGCACAGGA AGATCGGGAG GCCATTGAAG AAGCCATTCG CGGTGCCAAT ATGCTGTTTA TCACGACCGG TATGGGCGGC GGTACCGGTA CCGGTTCCGC GCCGGTTGTT GCTGAGATTG CCAAGTCTTT GGGCATTCTG ACCGTTGCCG TAGTTACCCG TCCGTTTTCT TATGAAGGCA AACGCGTCCA TGTGGCACAG GCAGGGTTGG AACAGTTGAA AGAATACGTC GATTCGCTGA TTATCATCCC GAACGACAAA CTGATGACCG CATTGGGTGA AGACGTAACT ATGCGCGAAG CCTTCCGCGC GGCGGACAAT GTATTGCGCG ATGCGGTCGC AGGCATTTCC GAAGTGGTAA CTTGCCCGAG CGAAATCATC AACCTCGACT TTGCCGACGT GAAAACCGTG ATGAGCAACC GCGGTATCGC TATGATGGGT TCGGGTTATG CCCAAGGTAT CGACCGTGCG CGTATGGCGA CCGACCAGGC CATTTCCAGT CCGTTGCTGG ACGATGTAAC TTTGGATGGT GCACGCGGTG TGCTGGTCAA TATTACGACT GCTCCGGGTT GCCTGAAAAT GTCCGAGTTG TCTGAAGTCA TGAAAATCGT CAACCAAAGC GCGCATCCCG ATTTGGAATG CAAATTCGGT GCCGCTGAAG ACGAGACCAT GAGCGAAGAT GCCATCCGGA TTACCATTAT CGCTACCGGT CTGAAAGAAA AAGGAGCGGT CGATCCTACT CCGGCAAGGG AAGTAGAAGC GGTTGCCCCG TCCAAACAGG AGCAAAGCCA CAATGTCGAA GGTATGATCC GCACCAATCG CGGTATCCGC ACCATGAATC TGACAGCAGC GGATTTCGAT AACCAGTCTG TCCTTGACGA TTTTGAAATT CCTGCAATTT TGCGTCGTCA ACACAATTCA GACAAATAA

Details for NegoA.00011.a.B1.GE39936

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/1/2016
EXPECTED MW:	43kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHME FVYDVAESAV SPAVIKVIGL GGGGCNAINN MVANNVRSVE FISANTDAQS LAKNHAAKRI QLGTNLTRGL GAGANPDIGR AAAQEDREAI EEAIRGANML FITTGMGGGT GTGSAPVVAE IAKSLGILTV AVVTRPFSYE GKRVHVAQAG LEQLKEYVDS LIIIPNDKLM TALGEDVTMR EAFRAADNVL RDAVAGISEV VTCPSEIINL DFADVKTVMS NRGIAMMGSG YAQGIDRARM ATDQAISSPL LDDVTLDGAR GVLVNITTAP GCLKMSELSE VMKIVNQSAH PDLECKFGAA EDETMSEDAI RITIIATGLK EKGAVDPTPA REVEAVAPSK QEQSHNVEGM IRTNRGIRTM NLTAADFDNQ SVLDDFEIPA

Validated NT Sequence

attgcaggaa tttcaaaatc gtcaaggaca gactggttat cgaaatccgc tgctgtcaga ttcatggtgc ggataccgcg attggtgcgg atcatacctt cgacattgtg gctttgctcc tgtttggacg gggcaaccgc ttctacttcc cttgccggag taggatcgac cgctcctttt tctttcagac cggtagcgat aatggtaatc cggatggcat cttcgctcat ggtctcgtct tcagcggcac cgaatttgca ttccaaatcg ggatgcgcgc tttggttgac gattttcatg acttcagaca actcggacat tttcaggcaa cccggagcag tcgtaatatt gaccagcaca ccgcgtgcac catccaaagt tacatcgtcc agcaacggac tggaaatggc ctggtcggtc gccatacgcg cacggtcgat accttgggca taacccgaac ccatcatagc gataccgcgg ttgctcatca cggttttcac gtcggcaaag tcgaggttga tgatttcgct cgggcaagtt accacttcgg aaatgcctgc gaccgcatcg cgcaatacat tgtccgccgc gcggaaggct tcgcgcatag ttacgtcttc acccaatgcg gtcatcagtt tgtcgttcgg gatgataatc agcgaatcga cgtattcttt caactgttcc aaccctgcct gtgccacatg gacgcgtttg ccttcataag aaaacggacg ggtaactacg gcaacggtca gaatgcccaa agacttggca atctcagcaa caaccggcgc ggaaccggta ccggtaccgc cgcccatacc ggtcgtgata aacagcatat tggcaccgcg aatggcttct tcaatggcct cccgatcttc ctgtgctgcc gcacggccga tatcgggatt cgcacccgcg cccaaaccgc gtgtcagatt cgtacccaac tggattctct tcgccgcatg gttttttgcc aaagactgcg catctgtatt ggcactgata aactccacac tgcgcacatt gttggcaacc atgttattga ttgcattgca accgccgccg cccaagccga ttactttaat taccgcaggg ctgactgccg attctgccac gtcgtaaaca aattccatat ggtggtggtg gtggtgagcc atggta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat ttgtttacga cgtggcagaa tcggcagtca gccctgcggt aattaaagta atcggcttgg gcggcggcgg ttgcaatgca atcaataaca tggttgccaa caatgtgcgc agtgtggagt ttatcagtgc caatacagat gcgcagtctt tggcaaaaaa ccatgcggcg aagagaatcc agttgggtac gaatctgaca cgcggtttgg gcgcgggtgc gaatcccgat atcggccgtg cggcagcaca ggaagatcgg gaggccattg aagaagccat tcgcggtgcc aatatgctgt ttatcacgac cggtatgggc ggcggtaccg gtaccggttc cgcgccggtt gttgctgaga ttgccaagtc tttgggcatt ctgaccgttg ccgtagttac ccgtccgttt tcttatgaag gcaaacgcgt ccatgtggca caggcagggt tggaacagtt gaaagaatac gtcgattcgc tgattatcat cccgaacgac aaactgatga ccgcattggg tgaagacgta actatgcgcg aagccttccg cgcggcggac aatgtattgc gcgatgcggt cgcaggcatt tccgaagtgg taacttgccc gagcgaaatc atcaacctcg actttgccga cgtgaaaacc gtgatgagca accgcggtat cgctatgatg ggttcgggtt atgcccaagg tatcgaccgt gcgcgtatgg cgaccgacca ggccatttcc agtccgttgc tggacgatgt aactttggat ggtgcacgcg gtgtgctggt caatattacg actgctccgg gttgcctgaa aatgtccgag ttgtctgaag tcatgaaaat cgtcaaccaa agcgcgcatc ccgatttgga atgcaaattc ggtgccgctg aagacgagac catgagcgaa gatgccatcc ggattaccat tatcgctacc ggtctgaaag aaaaaggagc ggtcgatcct actccggcaa gggaagtaga agcggttgcc ccgtccaaac aggagcaaag ccacaatgtc gaaggtatga tccgcaccaa tcgcggtatc cgcaccatga atctgacagc agcggatttc gataaccagt ctgtccttga cgattttgaa attcctgcaa ttttgcgtcg tcaacacaat tcagacaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat