NafoA.20238.a

deoxyhypusine hydroxylase

CENTER ID: NafoA.20238.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.20238.a.B3.GE43512 PSSpred predicted folded domain 34 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.20238.a.B3.PW38798 PSSpred predicted folded domain 34 355

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0079050

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVFDQASSS SNSNEELSNN KHTFNDPCDE FAPFSDSELK ELLQNDEIPV SKRSRMLYAL KQWTESEDKM KRAIDILSVG FKSKSALLRH EIAYVMGQMR SEIAIPILEN ILSNLSEDSM VRHEAGEALG AIGSPSALPT LEKFVNDQYR EVRETCELAI LNIQYQQEKA KNPQNNQDSI FSPFSSVDPA PACSDDKKLK FDDIKNTFLD LNKPLFERYK AMFTLRNIAS QYLELDKHDY MLCYKRKAQA IEVLCQGLLN PEEGALFKHE VAFVLGQIQE QSAADALEST LRDKNQHAMV RHEAAEALGS ISDPESLKLL EEFQADEVEA VKDSCVVAID MHNYWSAFLS LHKNNDEEEQ
NT Sequence
atgagcgtct ttgatcaggc ttcatcctcc tcgaattcta atgaagaact ttccaacaac aaacacacct tcaatgatcc atgcgatgaa tttgcaccat tcagcgattc tgaactcaaa gaacttttgc aaaatgacga aattccagtg agtaaacggt cccgaatgtt atatgctttg aaacaatgga cagaatcgga agacaagatg aaaagagcta ttgatattct gtcggttgga ttcaaaagta aaagtgcttt attgagacat gaaattgcct atgtgatggg acagatgcgt tctgaaattg ccatccccat tcttgagaat attttgagta atttgagtga agactcaatg gtacgacacg aagctggtga agccttgggt gccatcggaa gtccttctgc tctgccaact cttgagaaat ttgtgaatga tcaatataga gaagtgagag agacttgcga attggcaatt ttgaatattc aatatcaaca agaaaaggcc aagaatccac aaaataatca agactccatc ttttcaccat tctcgagtgt tgatcctgca cctgcctgct ctgatgacaa gaagctcaaa ttcgatgaca ttaagaatac tttcctcgat ttgaacaagc cattatttga aagatataag gccatgttta cattgagaaa cattgcttcc caatatctag aattagacaa gcacgattac atgttgtgct acaagagaaa ggctcaagct attgaagttt tatgtcaagg attattgaat ccagaagaag gagcactttt caaacatgaa gttgcgtttg tgttgggtca aattcaagaa caatccgccg ctgacgctct cgagtcaacc ttacgtgaca agaatcagca cgccatggtt cgacatgaag ccgcagaagc attgggttcc atttctgatc ctgaatcgct gaaacttttg gaagaattcc aagccgatga agttgaagct gtgaaggact catgtgtcgt tgccattgat atgcataatt attggtctgc ctttttgtca ttgcacaaga acaacgatga agaagagcaa taa
Details for NafoA.20238.a.B3.GE43512
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF SDSELKELLQ NDEIPVSKRS RMLYALKQWT ESEDKMKRAI DILSVGFKSK SALLRHEIAY VMGQMRSEIA IPILENILSN LSEDSMVRHE AGEALGAIGS PSALPTLEKF VNDQYREVRE TCELAILNIQ YQQEKAKNPQ NNQDSIFSPF SSVDPAPACS DDKKLKFDDI KNTFLDLNKP LFERYKAMFT LRNIASQYLE LDKHDYMLCY KRKAQAIEVL CQGLLNPEEG ALFKHEVAFV LGQIQEQSAA DALESTLRDK NQHAMVRHEA AEALGSISDP ESLKLLEEFQ ADEVEAVKDS CVVAIDMHNY WSAFLSLHKN N
Validated NT Sequence
ttactcactt agttgttctt gtgcaatgac aaaaaggcag accaataatt atgcatatca atggcaacga cacatgagtc cttcacagct tcaacttcat cggcttggaa ttcttccaaa agtttcagcg attcaggatc agaaatggaa cccaatgctt ctgcggcttc atgtcgaacc atggcgtgct gattcttgtc acgtaaggtt gactcgagag cgtcagcggc ggattgttct tgaatttgac ccaacacaaa cgcaacttca tgtttgaaaa gtgctccttc ttctggattc aataatcctt gacataaaac ttcaatagct tgagcctttc tcttgtagca caacatgtaa tcgtgcttgt ctaattctag atattgggaa gcaatgtttc tcaatgtaaa catggcctta tatctttcaa ataatggctt gttcaaatcg aggaaagtat tcttaatgtc atcgaatttg agcttcttgt catcagagca ggcaggtgca ggatcaacac tcgagaatgg tgaaaagatg gagtcttgat tattttgtgg attcttggcc ttttcttgtt gatattgaat attcaaaatt gccaattcgc aagtctctct cacttctcta tattgatcat tcacaaattt ctcaagagtt ggcagagcag aaggacttcc gatggcaccc aaggcttcac cagcttcgtg tcgtaccatt gagtcttcac tcaaattact caaaatattc tcaagaatgg ggatggcaat ttcagaacgc atctgtccca tcacataggc aatttcatgt ctcaataaag cacttttact tttgaatcca accgacagaa tatcaatagc tcttttcatc ttgtcttccg attctgtcca ttgtttcaaa gcatataaca ttcgggaccg tttactcact ggaatttcgt cattttgcaa aagttctttg agttcagaat cgctgaacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHFS DSELKELLQN DEIPVSKRSR MLYALKQWTE SEDKMKRAID ILSVGFKSKS ALLRHEIAYV MGQMRSEIAI PILENILSNL SEDSMVRHEA GEALGAIGSP SALPTLEKFV NDQYREVRET CELAILNIQY QQEKAKNPQN NQDSIFSPFS SVDPAPACSD DKKLKFDDIK NTFLDLNKPL FERYKAMFTL RNIASQYLEL DKHDYMLCYK RKAQAIEVLC QGLLNPEEGA LFKHEVAFVL GQIQEQSAAD ALESTLRDKN QHAMVRHEAA EALGSISDPE SLKLLEEFQA DEVEAVKDSC VVAIDMHNYW SAFLSLHKNN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttcagcg attctgaact caaagaactt ttgcaaaatg acgaaattcc agtgagtaaa cggtcccgaa tgttatatgc tttgaaacaa tggacagaat cggaagacaa gatgaaaaga gctattgata ttctgtcggt tggattcaaa agtaaaagtg ctttattgag acatgaaatt gcctatgtga tgggacagat gcgttctgaa attgccatcc ccattcttga gaatattttg agtaatttga gtgaagactc aatggtacga cacgaagctg gtgaagcctt gggtgccatc ggaagtcctt ctgctctgcc aactcttgag aaatttgtga atgatcaata tagagaagtg agagagactt gcgaattggc aattttgaat attcaatatc aacaagaaaa ggccaagaat ccacaaaata atcaagactc catcttttca ccattctcga gtgttgatcc tgcacctgcc tgctctgatg acaagaagct caaattcgat gacattaaga atactttcct cgatttgaac aagccattat ttgaaagata taaggccatg tttacattga gaaacattgc ttcccaatat ctagaattag acaagcacga ttacatgttg tgctacaaga gaaaggctca agctattgaa gttttatgtc aaggattatt gaatccagaa gaaggagcac ttttcaaaca tgaagttgcg tttgtgttgg gtcaaattca agaacaatcc gccgctgacg ctctcgagtc aaccttacgt gacaagaatc agcacgccat ggttcgacat gaagccgcag aagcattggg ttccatttct gatcctgaat cgctgaaact tttggaagaa ttccaagccg atgaagttga agctgtgaag gactcatgtg tcgttgccat tgatatgcat aattattggt ctgccttttt gtcattgcac aagaacaact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for NafoA.20238.a.B3.PW38798
PURIFICATION DATe: 2/4/2020
CONCENTRATION: 38mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF SDSELKELLQ NDEIPVSKRS RMLYALKQWT ESEDKMKRAI DILSVGFKSK SALLRHEIAY VMGQMRSEIA IPILENILSN LSEDSMVRHE AGEALGAIGS PSALPTLEKF VNDQYREVRE TCELAILNIQ YQQEKAKNPQ NNQDSIFSPF SSVDPAPACS DDKKLKFDDI KNTFLDLNKP LFERYKAMFT LRNIASQYLE LDKHDYMLCY KRKAQAIEVL CQGLLNPEEG ALFKHEVAFV LGQIQEQSAA DALESTLRDK NQHAMVRHEA AEALGSISDP ESLKLLEEFQ ADEVEAVKDS CVVAIDMHNY WSAFLSLHKN N
Validated NT Sequence
ttactcactt agttgttctt gtgcaatgac aaaaaggcag accaataatt atgcatatca atggcaacga cacatgagtc cttcacagct tcaacttcat cggcttggaa ttcttccaaa agtttcagcg attcaggatc agaaatggaa cccaatgctt ctgcggcttc atgtcgaacc atggcgtgct gattcttgtc acgtaaggtt gactcgagag cgtcagcggc ggattgttct tgaatttgac ccaacacaaa cgcaacttca tgtttgaaaa gtgctccttc ttctggattc aataatcctt gacataaaac ttcaatagct tgagcctttc tcttgtagca caacatgtaa tcgtgcttgt ctaattctag atattgggaa gcaatgtttc tcaatgtaaa catggcctta tatctttcaa ataatggctt gttcaaatcg aggaaagtat tcttaatgtc atcgaatttg agcttcttgt catcagagca ggcaggtgca ggatcaacac tcgagaatgg tgaaaagatg gagtcttgat tattttgtgg attcttggcc ttttcttgtt gatattgaat attcaaaatt gccaattcgc aagtctctct cacttctcta tattgatcat tcacaaattt ctcaagagtt ggcagagcag aaggacttcc gatggcaccc aaggcttcac cagcttcgtg tcgtaccatt gagtcttcac tcaaattact caaaatattc tcaagaatgg ggatggcaat ttcagaacgc atctgtccca tcacataggc aatttcatgt ctcaataaag cacttttact tttgaatcca accgacagaa tatcaatagc tcttttcatc ttgtcttccg attctgtcca ttgtttcaaa gcatataaca ttcgggaccg tttactcact ggaatttcgt cattttgcaa aagttctttg agttcagaat cgctgaacat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHFS DSELKELLQN DEIPVSKRSR MLYALKQWTE SEDKMKRAID ILSVGFKSKS ALLRHEIAYV MGQMRSEIAI PILENILSNL SEDSMVRHEA GEALGAIGSP SALPTLEKFV NDQYREVRET CELAILNIQY QQEKAKNPQN NQDSIFSPFS SVDPAPACSD DKKLKFDDIK NTFLDLNKPL FERYKAMFTL RNIASQYLEL DKHDYMLCYK RKAQAIEVLC QGLLNPEEGA LFKHEVAFVL GQIQEQSAAD ALESTLRDKN QHAMVRHEAA EALGSISDPE SLKLLEEFQA DEVEAVKDSC VVAIDMHNYW SAFLSLHKNN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTCAGCG ATTCTGAACT CAAAGAACTT TTGCAAAATG ACGAAATTCC AGTGAGTAAA CGGTCCCGAA TGTTATATGC TTTGAAACAA TGGACAGAAT CGGAAGACAA GATGAAAAGA GCTATTGATA TTCTGTCGGT TGGATTCAAA AGTAAAAGTG CTTTATTGAG ACATGAAATT GCCTATGTGA TGGGACAGAT GCGTTCTGAA ATTGCCATCC CCATTCTTGA GAATATTTTG AGTAATTTGA GTGAAGACTC AATGGTACGA CACGAAGCTG GTGAAGCCTT GGGTGCCATC GGAAGTCCTT CTGCTCTGCC AACTCTTGAG AAATTTGTGA ATGATCAATA TAGAGAAGTG AGAGAGACTT GCGAATTGGC AATTTTGAAT ATTCAATATC AACAAGAAAA GGCCAAGAAT CCACAAAATA ATCAAGACTC CATCTTTTCA CCATTCTCGA GTGTTGATCC TGCACCTGCC TGCTCTGATG ACAAGAAGCT CAAATTCGAT GACATTAAGA ATACTTTCCT CGATTTGAAC AAGCCATTAT TTGAAAGATA TAAGGCCATG TTTACATTGA GAAACATTGC TTCCCAATAT CTAGAATTAG ACAAGCACGA TTACATGTTG TGCTACAAGA GAAAGGCTCA AGCTATTGAA GTTTTATGTC AAGGATTATT GAATCCAGAA GAAGGAGCAC TTTTCAAACA TGAAGTTGCG TTTGTGTTGG GTCAAATTCA AGAACAATCC GCCGCTGACG CTCTCGAGTC AACCTTACGT GACAAGAATC AGCACGCCAT GGTTCGACAT GAAGCCGCAG AAGCATTGGG TTCCATTTCT GATCCTGAAT CGCTGAAACT TTTGGAAGAA TTCCAAGCCG ATGAAGTTGA AGCTGTGAAG GACTCATGTG TCGTTGCCAT TGATATGCAT AATTATTGGT CTGCCTTTTT GTCATTGCAC AAGAACAACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT