NafoA.19900.a
Glucokinase (NfHK)
| CENTER ID: | NafoA.19900.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.19900.a.B1.GE42084 | full length | 1 | 441 | ||
| NafoA.19900.a.B11.GE42085 | full length | 1 | 441 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.19900.a.B11.PW38443 | full length | 1 | 441 |
Structures
6DA0
DEPOSITED: 5/1/2018
DETERMINATION: XRay
CLONE: NafoA.19900.a.B11.GE42085
PROTEIN: NafoA.19900.a.B11.PW38443
Publications by SSGCID
Enzymatic and structural characterization of the Naegleria fowleri glucokinase
Abendroth J, Dranow DM, Phan IQ, Van Voorhis W, Milanes JE, Suryadi J, Barrett KF, Patrick SL, Khalifa MM, Golden JE, Morris JC, Rozema S
Antimicrob. Agents Chemother. - 2021
volume 63, issue 5, pages e02410-18
PMID: 30783001; PMCID: PMC6496100
PDB:
6DA0
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0035880 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPERMISSSS RSSMSSSTSS EEPLVIDEDF INQVEKHYRK SLKNIPFQYI VGVDVGATNT RIAIQFIINE DQDDEVFMTK FPCNTSTHLA NYLAAYGKAM VKAVGKGSAA GSIALAGPVT GDKVRITNYK EHDQEFFYSQ LPDTLFPASK NTFLNDLEAS CYGIINVGTN NRLHEFFCPI DALNNYATSQ TVRLSDTSEY AVLAMGTGLG TGLIVGSAGG KFNVIPLEAG HVHIATPGVN SEHFKEERER IEFLSQKIYG GAYPIEYEDI CSGRGLEFCY EFEIRNDPNA VRKTASQIAE SYSTDTYARQ AMITHYRYLM KAAQNIAVLI PTCRGVFFAG DNQVFNEDFF KEHLSILQKE LFQTHQKKHW LTDLKPYRQM KEYNFNVKGC LQKARELAQL GSTTMVDEKP KAGLKDVLSV AIPTAFFSVL SFFTAKNLYE K
NT Sequence
atgcccgaaa gaatgatttc atcgtccagt agaagcagta tgagtagtag cacaagctca gaggagcctc ttgtgattga tgaagacttt atcaatcaag ttgaaaaaca ctaccgaaag tcccttaaaa acattccgtt tcaatacatt gtaggtgttg atgtgggagc caccaacaca agaattgcca ttcaatttat catcaatgaa gaccaagacg atgaagtatt catgaccaag ttcccatgta atacttccac tcatttggca aattatcttg cagcgtatgg aaaggctatg gtgaaagctg tcggaaaggg aagtgctgct ggttctattg ccctcgcagg tccagtcact ggagacaaag taagaattac aaattacaag gaacatgacc aagagttttt ctattcacaa cttccagata cattgttccc agccagtaag aatacttttt tgaacgatct tgaagcgagt tgctacggaa ttattaatgt tggaacgaac aatcgtttac atgaattctt ctgcccaatt gatgctttga ataattatgc aacttcacaa actgtacgac tttctgatac cagtgaatat gctgtgttgg cgatgggaac tggtcttgga acaggattaa ttgttggttc tgctggagga aaattcaatg tcattcctct cgaagcagga cacgtgcata ttgccactcc tggcgtaaat agtgaacatt tcaaggaaga acgtgagaga attgaattct tgagccaaaa aatttatgga ggtgcctatc caattgagta tgaggatatt tgcagtggaa gaggtttaga attttgctat gagtttgaaa ttagaaatga tccaaatgct gtaagaaaaa cagcttctca aattgccgaa tcttactcca ctgacacata tgcaagacaa gccatgatta ctcattatcg atatttaatg aaggccgctc aaaatattgc agttctcatt ccaacctgtc gtggagtttt cttcgcaggt gacaatcaag tatttaatga agatttcttt aaggaacatc tttctatctt acaaaaagaa ctcttccaaa cacaccaaaa gaaacattgg cttactgatt tgaagccata cagacaaatg aaagaataca actttaatgt gaagggttgt cttcaaaaag caagagaatt agcccaactt ggttctacta ccatggttga tgaaaagcca aaagctggac tcaaagatgt tctttctgtg gccataccaa ctgctttctt ctcagtcctt tcattcttca ctgctaagaa tttgtatgaa aagtaa
Details for NafoA.19900.a.B1.GE42084
| HARVESTED ON: | 12/5/2017 |
| SEQUENCED ON: | 12/7/2017 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP ERMISSSSRS SMSSSTSSEE PLVIDEDFIN QVEKHYRKSL KNIPFQYIVG VDVGATNTRI AIQFIINEDQ DDEVFMTKFP CNTSTHLANY LAAYGKAMVK AVGKGSAAGS IALAGPVTGD KVRITNYKEH DQEFFYSQLP DTLFPASKNT FLNDLEASCY GIINVGTNNR LHEFFCPIDA LNNYATSQTV RLSDTSEYAV LAMGTGLGTG LIVGSAGGKF NVIPLEAGHV HIATPGVNSE HFKEERERIE FLSQKIYGGA YPIEYEDICS GRGLEFCYEF EIRNDPNAVR KTASQIAESY STDTYARQAM ITHYRYLMKA AQNIAVLIPT CRGVFFAGDN QVFNEDFFKE HLSILQKELF QTHQKKHWLT DLKPYRQMKE YNFNVKGCLQ KARELAQLGS TTMVDEKPKA GLKDVLSVAI PTAFFSVLSF FTAKNLYEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg aaagaatgat ttcatcgtcc agtagaagca gtatgagtag tagcacaagc tcagaggagc ctcttgtgat tgatgaagac tttatcaatc aagttgaaaa acactaccga aagtccctta aaaacattcc gtttcaatac attgtaggtg ttgatgtggg agccaccaac acaagaattg ccattcaatt tatcatcaat gaagaccaag acgatgaagt attcatgacc aagttcccat gtaatacttc cactcatttg gcaaattatc ttgcagcgta tggaaaggct atggtgaaag ctgtcggaaa gggaagtgct gctggttcta ttgccctcgc aggtccagtc actggagaca aagtaagaat tacaaattac aaggaacatg accaagagtt tttctattca caacttccag atacattgtt cccagccagt aagaatactt ttttgaacga tcttgaagcg agttgctacg gaattattaa tgttggaacg aacaatcgtt tacatgaatt cttctgccca attgatgctt tgaataatta tgcaacttca caaactgtac gactttctga taccagtgaa tatgctgtgt tggcgatggg aactggtctt ggaacaggat taattgttgg ttctgctgga ggaaaattca atgtcattcc tctcgaagca ggacacgtgc atattgccac tcctggcgta aatagtgaac atttcaagga agaacgtgag agaattgaat tcttgagcca aaaaatttat ggaggtgcct atccaattga gtatgaggat atttgcagtg gaagaggttt agaattttgc tatgagtttg aaattagaaa tgatccaaat gctgtaagaa aaacagcttc tcaaattgcc gaatcttact ccactgacac atatgcaaga caagccatga ttactcatta tcgatattta atgaaggccg ctcaaaatat tgcagttctc attccaacct gtcgtggagt tttcttcgca ggtgacaatc aagtatttaa tgaagatttc tttaaggaac atctttctat cttacaaaaa gaactcttcc aaacacacca aaagaaacat tggcttactg atttgaagcc atacagacaa atgaaagaat acaactttaa tgtgaagggt tgtcttcaaa aagcaagaga attagcccaa cttggttcta ctaccatggt tgatgaaaag ccaaaagctg gactcaaaga tgttctttct gtggccatac caactgcttt cttctcagtc ctttcattct tcactgctaa gaatttgtat gaaaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NafoA.19900.a.B11.GE42085
| HARVESTED ON: | 12/5/2017 |
| SEQUENCED ON: | 12/7/2017 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP ERMISSSSRS SMSSSTSSEE PLVIDEDFIN QVEKHYRKSL KNIPFQYIVG VDVGATNTRI AIQFIINEDQ DDEVFMTKFP CNTSTHLANY LAAYGKAMVK AVGKGSAAGS IALAGPVTGD KVRITNYKEH DQEFFYSQLP DTLFPASKNT FLNDLEASCY GIINVGTNNR LHEFFCPIDA LNNYATSQTV RLSDTSEYAV LAMGTGLGTG LIVGSAGGKF NVIPLEAGHV HIATPGVNSE HFKEERERIE FLSQKIYGGA YPIEYEDICS GRGLEFCYEF EIRNDPNAVR KTASQIAESY STDTYARQAM ITHYRYLMKA AQNIAVLIPT CRGVFFAGDN QVFNEDFFKE HLSILQKELF QTHQKKHWLT DLKPYRQMKE YNFNVKGCLQ KARELAQLGS TTMVDEKPKA GLKDVLSVAI PTAFFSVLSF FTAKNLYEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg aaagaatgat ttcatcgtcc agtagaagca gtatgagtag tagcacaagc tcagaggagc ctcttgtgat tgatgaagac tttatcaatc aagttgaaaa acactaccga aagtccctta aaaacattcc gtttcaatac attgtaggtg ttgatgtggg agccaccaac acaagaattg ccattcaatt tatcatcaat gaagaccaag acgatgaagt attcatgacc aagttcccat gtaatacttc cactcatttg gcaaattatc ttgcagcgta tggaaaggct atggtgaaag ctgtcggaaa gggaagtgct gctggttcta ttgccctcgc aggtccagtc actggagaca aagtaagaat tacaaattac aaggaacatg accaagagtt tttctattca caacttccag atacattgtt cccagccagt aagaatactt ttttgaacga tcttgaagcg agttgctacg gaattattaa tgttggaacg aacaatcgtt tacatgaatt cttctgccca attgatgctt tgaataatta tgcaacttca caaactgtac gactttctga taccagtgaa tatgctgtgt tggcgatggg aactggtctt ggaacaggat taattgttgg ttctgctgga ggaaaattca atgtcattcc tctcgaagca ggacacgtgc atattgccac tcctggcgta aatagtgaac atttcaagga agaacgtgag agaattgaat tcttgagcca aaaaatttat ggaggtgcct atccaattga gtatgaggat atttgcagtg gaagaggttt agaattttgc tatgagtttg aaattagaaa tgatccaaat gctgtaagaa aaacagcttc tcaaattgcc gaatcttact ccactgacac atatgcaaga caagccatga ttactcatta tcgatattta atgaaggccg ctcaaaatat tgcagttctc attccaacct gtcgtggagt tttcttcgca ggtgacaatc aagtatttaa tgaagatttc tttaaggaac atctttctat cttacaaaaa gaactcttcc aaacacacca aaagaaacat tggcttactg atttgaagcc atacagacaa atgaaagaat acaactttaa tgtgaagggt tgtcttcaaa aagcaagaga attagcccaa cttggttcta ctaccatggt tgatgaaaag ccaaaagctg gactcaaaga tgttctttct gtggccatac caactgcttt cttctcagtc ctttcattct tcactgctaa gaatttgtat gaaaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NafoA.19900.a.B11.PW38443
| PURIFICATION DATe: | 3/19/2018 |
| CONCENTRATION: | 13.78mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMP ERMISSSSRS SMSSSTSSEE PLVIDEDFIN QVEKHYRKSL KNIPFQYIVG VDVGATNTRI AIQFIINEDQ DDEVFMTKFP CNTSTHLANY LAAYGKAMVK AVGKGSAAGS IALAGPVTGD KVRITNYKEH DQEFFYSQLP DTLFPASKNT FLNDLEASCY GIINVGTNNR LHEFFCPIDA LNNYATSQTV RLSDTSEYAV LAMGTGLGTG LIVGSAGGKF NVIPLEAGHV HIATPGVNSE HFKEERERIE FLSQKIYGGA YPIEYEDICS GRGLEFCYEF EIRNDPNAVR KTASQIAESY STDTYARQAM ITHYRYLMKA AQNIAVLIPT CRGVFFAGDN QVFNEDFFKE HLSILQKELF QTHQKKHWLT DLKPYRQMKE YNFNVKGCLQ KARELAQLGS TTMVDEKPKA GLKDVLSVAI PTAFFSVLSF FTAKNLYEK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCG AAAGAATGAT TTCATCGTCC AGTAGAAGCA GTATGAGTAG TAGCACAAGC TCAGAGGAGC CTCTTGTGAT TGATGAAGAC TTTATCAATC AAGTTGAAAA ACACTACCGA AAGTCCCTTA AAAACATTCC GTTTCAATAC ATTGTAGGTG TTGATGTGGG AGCCACCAAC ACAAGAATTG CCATTCAATT TATCATCAAT GAAGACCAAG ACGATGAAGT ATTCATGACC AAGTTCCCAT GTAATACTTC CACTCATTTG GCAAATTATC TTGCAGCGTA TGGAAAGGCT ATGGTGAAAG CTGTCGGAAA GGGAAGTGCT GCTGGTTCTA TTGCCCTCGC AGGTCCAGTC ACTGGAGACA AAGTAAGAAT TACAAATTAC AAGGAACATG ACCAAGAGTT TTTCTATTCA CAACTTCCAG ATACATTGTT CCCAGCCAGT AAGAATACTT TTTTGAACGA TCTTGAAGCG AGTTGCTACG GAATTATTAA TGTTGGAACG AACAATCGTT TACATGAATT CTTCTGCCCA ATTGATGCTT TGAATAATTA TGCAACTTCA CAAACTGTAC GACTTTCTGA TACCAGTGAA TATGCTGTGT TGGCGATGGG AACTGGTCTT GGAACAGGAT TAATTGTTGG TTCTGCTGGA GGAAAATTCA ATGTCATTCC TCTCGAAGCA GGACACGTGC ATATTGCCAC TCCTGGCGTA AATAGTGAAC ATTTCAAGGA AGAACGTGAG AGAATTGAAT TCTTGAGCCA AAAAATTTAT GGAGGTGCCT ATCCAATTGA GTATGAGGAT ATTTGCAGTG GAAGAGGTTT AGAATTTTGC TATGAGTTTG AAATTAGAAA TGATCCAAAT GCTGTAAGAA AAACAGCTTC TCAAATTGCC GAATCTTACT CCACTGACAC ATATGCAAGA CAAGCCATGA TTACTCATTA TCGATATTTA ATGAAGGCCG CTCAAAATAT TGCAGTTCTC ATTCCAACCT GTCGTGGAGT TTTCTTCGCA GGTGACAATC AAGTATTTAA TGAAGATTTC TTTAAGGAAC ATCTTTCTAT CTTACAAAAA GAACTCTTCC AAACACACCA AAAGAAACAT TGGCTTACTG ATTTGAAGCC ATACAGACAA ATGAAAGAAT ACAACTTTAA TGTGAAGGGT TGTCTTCAAA AAGCAAGAGA ATTAGCCCAA CTTGGTTCTA CTACCATGGT TGATGAAAAG CCAAAAGCTG GACTCAAAGA TGTTCTTTCT GTGGCCATAC CAACTGCTTT CTTCTCAGTC CTTTCATTCT TCACTGCTAA GAATTTGTAT GAAAAGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T