NafoA.19532.b

putative heterotrimeric G protein effector RGS-RhoGEF

CENTER ID: NafoA.19532.b
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NafoA.19532.b.B2.GE41668 RGS domain 590 719
NafoA.19532.b.B21.GE42360 RGS domain 590 719
NafoA.19532.b.B22.GE42361 RGS domain 590 719
NafoA.19532.b.B23.GE42362 RGS domain 590 719
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0047690

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKLLILQAL ISSSAARKRL LSRKAEHGHH MAVSPVIQSA LRGYLWRKNS LLYIPNTNLE IAPISITSML DTMNKLFDEE DDSDELDSSD SPNLNVEATK AVPLPARGES TMTPKVDIYD FSKNMDTGFG GSKKIVQLIR DRLNNKSNIL DLSGLCIESL PNIPFDCDHV TELNLSGNLL VTLPEWFCDK FKNVKILNLS RNRFFDANRL LSKNLIPLSY TLTHLDLSKN RFEYFEYIPD DEKYDVFSNS FQELQYLNLS HNQLLNISGE IFDLISNHKL ESLDLSHNQL MTFPTVSSSD LLKKWKGFKF INLEHNSFLQ SEVGKILYMF KKRPDILKLL KVQLTKRGTQ HDESKTAKKA IVETASIDSG DFFSSSLFDD DNQDDSQPLS DLTELAYHNR FKTHPSTFTF NNTSTDRTFA EYDSDEDESL MTDHSFYQPH NSGANSPNFL SVISANQPTS PSSVTTMNDS SFDCSPFLSD REFITPTKLS VEVAGEDSSP LPKLNVVDPI FDDTSMEHSV DTSNDTTVPL AIHITTPTSG QTKLNFIQDL NIPHVTSTTT PNSPSEESKP KIPKLNLATS PLPTISSQLP SFEKEVQFLS SRSTTSDPMS SPSVPSPAND SENLVSVLNN ENIRKSFKIF CKSEFSVESI DFYEEVCAFK QLKDVTLIRN TSQHIFSTFI DPFGIHSLNI NTKQVESVKN LIMENGKLKE NISIDVFDSI LQLVKMNLLD PFKRFQLSDD YKLYDEQMKT PKTARGTPLP RKLTTRHPLL RNEEEQKRLS TKRKKIPFPD VTPKPSLAEE RKSVNQISVQ LPERKSINVK QMKERVNNLM EILHSERRYL EFLIDLWEMY YVPLVENRYI KGTEQPKRKS KGQTEETQQP SLKRKLSFVD KLFNKNKNNS NANEDNRIVS KADAQEMFPK NLFSIITFNK GFLVKLEERF SLYSNDEDNG TVILPRMYNM MIGDIFIKSI PYFRLYDNYL ESYENAMNKI RQVRVQNKEF DRWIKRRKSH PRSRNLEIHS FLVCPVQRIV RYKLLIENLL KTTEEDHPDF FPLTKALEMI IDMANQQNIS IKLTNNRVKV RMLTQLFDLY EQLGCSSTQS GAVVEDNLLL REGDVEISIS KEESTSPKKH KQSQRAYLFS NVLVLIEKTK MVDKQISSSD NTTNPNGAAQ GETNSQDEPL KRSSSFWKKK KESPSGQTSP KIHKAIKVEK KHVRLEDAFI SGGSSNTIEI SFTVGSKLPS VSSENDLKGS HSALSTDTLS ASHLVSMSGL FSARKHSKST DNIPSNSTSS LDSARKRSAS LFEKLTSKYK LKQKDTGTKL TLVLSFVSSE LKNQWLSDLE QAIKQAKLKQ LSKASKLNAS SSFII
NT Sequence
atgaataagc tactcattct acaagctctc atatctagct cagcagcacg aaaaaggcta ctctcaagga aagccgagca tggacaccat atggcagtct cacctgtgat tcaatcggct ttgagaggat atttatggag aaaaaactct ctgttataca ttccaaacac caatctagag attgctccta tttctattac ctccatgcta gataccatga acaagctctt tgatgaagaa gatgactcgg atgaattgga ttcttctgac tctcccaatt tgaatgtgga agctacaaaa gccgttcctt tgccagcaag aggtgaaagt acaatgactc caaaagttga tatttatgat ttttccaaga atatggacac tggatttggt ggatcgaaaa aaattgttca actcattaga gatcgactca ataacaaatc taacatacta gatctttcag gactatgcat tgagagtctt ccaaatattc catttgattg tgaccacgtt acagagctga acttgtctgg aaacttatta gtcacactcc ctgaatggtt ctgtgacaag ttcaaaaatg tgaaaatttt aaatttgagt agaaatagat tttttgatgc taatcgccta ctttctaaga accttattcc attgtcatat accttgactc acctcgattt aagtaagaat agatttgaat attttgaata cattcctgat gatgaaaaat atgatgtctt tagcaactcc ttccaagaat tgcagtactt gaatttgagt cacaaccagt tgctgaatat atcaggagaa atctttgacc tcattagtaa tcacaaattg gaaagtttgg atctctctca taaccaatta atgacttttc caactgtgtc ctcatcagat cttttaaaaa agtggaaagg ttttaaattt attaacttgg aacacaactc tttcctccaa tctgaggttg gaaaaatttt gtacatgttt aaaaagcgac cagatattct caaattgttg aaagtgcagt taaccaaacg tggtacacaa catgacgaat caaaaactgc aaagaaagcc attgtggaaa ctgcctcaat agattctggg gactttttct ctagcagcct cttcgacgac gataatcaag acgattcaca accactatcg gatttgacgg agttagccta tcacaacaga ttcaagaccc acccttcaac ctttacgttc aacaatactt ccactgacag aacatttgcc gagtatgatt cagatgaaga tgagtctttg atgacagatc actcctttta tcagcctcac aactcgggcg ctaattctcc aaactttctc tccgttataa gtgcaaatca acccacttca ccatcttcag ttactacgat gaacgacagc agtttcgatt gctctccatt tctgtcagat cgagaattca taacaccaac caaattgtct gtagaagtag caggagaaga ctcatcaccg cttccaaagc taaatgttgt agatcctatc tttgatgata cttcaatgga acactctgtc gatacatcaa atgacacaac tgttccatta gccattcata tcacaactcc aacttctgga caaaccaagt tgaattttat tcaagacctt aatattcctc atgtcacctc taccacaact ccaaactcac caagtgaaga atccaaacca aaaattccca agcttaatct tgcaacttca ccactcccaa caatttccag tcaactacca tcgtttgaaa aagaagttca gttcctttcc tcaaggtcca ctacttcgga tccaatgagc tctccctcag ttccaagccc tgcgaatgat tcggaaaatc ttgtgtctgt tctcaataat gagaatattc gaaaatcatt caaaatattt tgtaaatcag aatttagtgt ggaatctatt gacttttatg aagaagtttg tgcatttaaa caactcaaag atgtcactct tattcgtaac acatcccagc acatctttag cacctttata gatccttttg gaattcactc cttgaatatt aatactaagc aagttgaatc tgtgaaaaac ctcatcatgg agaatggaaa gttgaaggag aatatttcta tcgatgtttt tgattccata cttcaacttg taaagatgaa cctacttgat ccattcaaga gattccagct ttcagacgat tacaaattgt atgatgagca aatgaaaaca cccaaaacag ctcgaggaac accactaccc agaaagctca ccactcgcca tccactcttg cgaaatgagg aagaacaaaa acggttatct acaaaacgaa aaaagattcc cttccccgat gtcactccaa agccatcact tgctgaagaa agaaagagtg tgaaccaaat ttcggtgcag ttacctgaaa gaaagagcat taatgttaaa caaatgaaag agagagttaa caaccttatg gaaattctac atagtgaacg aaggtatttg gagttcttaa ttgatctttg ggagatgtat tatgttcctc ttgttgagaa ccgatatatt aaaggcacag aacaaccaaa gcgaaaatcg aaaggccaga ccgaagagac gcaacaaccc tctctcaaga gaaaactgtc ctttgtcgac aaactcttca acaagaacaa gaataactcc aatgcgaatg aagacaatcg aatcgtatcc aaagcagatg ctcaagaaat gtttccaaag aatctctttt caattattac gttcaataag ggcttcctcg ttaaattaga agaacgtttt tccttgtaca gcaatgatga agacaatggc actgtcattt tgcctcggat gtataacatg atgattggag atattttcat taagagcatt ccgtacttta ggttatatga caattatttg gagagttatg aaaatgcaat gaacaagatt agacaagtac gagtccaaaa caaggaattc gatcgatgga ttaaaaggag aaaatcccac cctcggtctc gtaaccttga aattcacagc tttttagtat gtccagttca aagaattgtc agatacaagt tgctaattga gaatttgctg aaaacaactg aagaggatca tcctgatttc tttcctttga ccaaagcttt ggaaatgatt attgacatgg ccaatcagca aaatattagt atcaagttga caaataatcg agttaaggtg agaatgttaa cacaattatt tgatctatat gaacaactcg gttgctcatc aactcaaagt ggtgccgttg ttgaggataa tttactactg cgtgaaggag atgtagaaat atctattagt aaggaagaga gtacatctcc gaaaaagcac aagcaatctc aaagagcata tctatttagc aatgtgctag tgctcattga gaaaactaag atggtcgaca agcaaatttc ctcatccgat aataccacaa atccaaatgg agccgctcaa ggagaaacca attcacagga tgagcccctg aaacgaagtt cttccttctg gaagaaaaaa aaggagtcac cgtccggaca aaccagtcca aaaattcaca aagccattaa ggttgaaaag aagcatgttc gattggaaga tgcattcatt tcgggaggct cctcaaacac gattgaaatt tctttcacag ttggctcaaa acttccatct gtgtcatctg aaaatgatct caaaggttct cactcagctc taagcacaga cactctttct gcatcacatc ttgtctccat gagtggacta ttctcagctc gaaaacatag caaatcaacc gataatattc catccaactc gacgagctca ctcgattctg ctcgtaaacg atcggcaagt ctttttgaaa aactcacaag caagtacaaa cttaaacaaa aggatacagg taccaaatta actttggtat tgtcatttgt aagcagtgaa ttgaagaatc aatggctctc agatttggaa caagcaatca aacaagccaa attgaagcaa ctctcaaaag cttccaaact gaatgcttcc tccagtttta tcatttaa
Details for NafoA.19532.b.B2.GE41668
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 16kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP SFEKEVQFLS SRSTTSDPMS SPSVPSPAND SENLVSVLNN ENIRKSFKIF CKSEFSVESI DFYEEVCAFK QLKDVTLIRN TSQHIFSTFI DPFGIHSLNI NTKQVESVKN LIMENGKLKE NISIDVFDS
Validated NT Sequence
atcttactca cttaggaatc aaaaacatcg atagaaatat tctccttcaa ctttccattc tccatgatga ggtttttcac agattcaact tgcttagtat taatattcaa ggagtgaatt ccaaaaggat ctataaaggt gctaaagatg tgctgggatg tgttacgaat aagagtgaca tctttgagtt gtttaaatgc acaaacttct tcataaaagt caatagattc cacactaaat tctgatttac aaaatatttt gaatgatttt cgaatattct cattattgag aacagacaca agattttccg aatcattcgc agggcttgga actgagggag agctcattgg atccgaagta gtggaccttg aggaaaggaa ctgaacttct ttttcaaacg atggcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHPS FEKEVQFLSS RSTTSDPMSS PSVPSPANDS ENLVSVLNNE NIRKSFKIFC KSEFSVESID FYEEVCAFKQ LKDVTLIRNT SQHIFSTFID PFGIHSLNIN TKQVESVKNL IMENGKLKEN ISIDVFDS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccatcgt ttgaaaaaga agttcagttc ctttcctcaa ggtccactac ttcggatcca atgagctctc cctcagttcc aagccctgcg aatgattcgg aaaatcttgt gtctgttctc aataatgaga atattcgaaa atcattcaaa atattttgta aatcagaatt tagtgtggaa tctattgact tttatgaaga agtttgtgca tttaaacaac tcaaagatgt cactcttatt cgtaacacat cccagcacat ctttagcacc tttatagatc cttttggaat tcactccttg aatattaata ctaagcaagt tgaatctgtg aaaaacctca tcatggagaa tggaaagttg aaggagaata tttctatcga tgtttttgat tcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.19532.b.B21.GE42360
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 16kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHPS FEKEVQFLSS RSTTSDPMSS PSVPSPANDS ENLVSVLNNE NIRKSFKIFC KSEFSVESID FYEEVCAFKQ LKDVTLIRNT SQHIFSTFID PFGIHSLNIN TKQVESVKNL IMENGKLKEN ISIDVFDS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccatcgt ttgaaaaaga agttcagttc ctttcctcaa ggtccactac ttcggatcca atgagctctc cctcagttcc aagccctgcg aatgattcgg aaaatcttgt gtctgttctc aataatgaga atattcgaaa atcattcaaa atattttgta aatcagaatt tagtgtggaa tctattgact tttatgaaga agtttgtgca tttaaacaac tcaaagatgt cactcttatt cgtaacacat cccagcacat ctttagcacc tttatagatc cttttggaat tcactccttg aatattaata ctaagcaagt tgaatctgtg aaaaacctca tcatggagaa tggaaagttg aaggagaata tttctatcga tgtttttgat tcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.19532.b.B22.GE42361
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 16kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHPS FEKEVQFLSS RSTTSDPMSS PSVPSPANDS ENLVSVLNNE NIRKSFKIFC KSEFSVESID FYEEVCAFKQ LKDVTLIRNT SQHIFSTFID PFGIHSLNIN TKQVESVKNL IMENGKLKEN ISIDVFDS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccatcgt ttgaaaaaga agttcagttc ctttcctcaa ggtccactac ttcggatcca atgagctctc cctcagttcc aagccctgcg aatgattcgg aaaatcttgt gtctgttctc aataatgaga atattcgaaa atcattcaaa atattttgta aatcagaatt tagtgtggaa tctattgact tttatgaaga agtttgtgca tttaaacaac tcaaagatgt cactcttatt cgtaacacat cccagcacat ctttagcacc tttatagatc cttttggaat tcactccttg aatattaata ctaagcaagt tgaatctgtg aaaaacctca tcatggagaa tggaaagttg aaggagaata tttctatcga tgtttttgat tcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.19532.b.B23.GE42362
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 16kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP SFEKEVQFLS SRSTTSDPMS SPSVPSPAND SENLVSVLNN ENIRKSFKIF CKSEFSVESI DFYEEVCAFK QLKDVTLIRN TSQHIFSTFI DPFGIHSLNI NTKQVESVKN LIMENGKLKE NISIDVFDS
Validated NT Sequence
atcttactca cttaggaatc aaaaacatcg atagaaatat tctccttcaa ctttccattc tccatgatga ggtttttcac agattcaact tgcttagtat taatattcaa ggagtgaatt ccaaaaggat ctataaaggt gctaaagatg tgctgggatg tgttacgaat aagagtgaca tctttgagtt gtttaaatgc acaaacttct tcataaaagt caatagattc cacactaaat tctgatttac aaaatatttt gaatgatttt cgaatattct cattattgag aacagacaca agattttccg aatcattcgc agggcttgga actgagggag agctcattgg atccgaagta gtggaccttg aggaaaggaa ctgaacttct ttttcaaacg atggcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHPS FEKEVQFLSS RSTTSDPMSS PSVPSPANDS ENLVSVLNNE NIRKSFKIFC KSEFSVESID FYEEVCAFKQ LKDVTLIRNT SQHIFSTFID PFGIHSLNIN TKQVESVKNL IMENGKLKEN ISIDVFDS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccatcgt ttgaaaaaga agttcagttc ctttcctcaa ggtccactac ttcggatcca atgagctctc cctcagttcc aagccctgcg aatgattcgg aaaatcttgt gtctgttctc aataatgaga atattcgaaa atcattcaaa atattttgta aatcagaatt tagtgtggaa tctattgact tttatgaaga agtttgtgca tttaaacaac tcaaagatgt cactcttatt cgtaacacat cccagcacat ctttagcacc tttatagatc cttttggaat tcactccttg aatattaata ctaagcaagt tgaatctgtg aaaaacctca tcatggagaa tggaaagttg aaggagaata tttctatcga tgtttttgat tcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat