NafoA.19532.a

putative RhoGEF domains (DH-PH tandem)

CENTER ID: NafoA.19532.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.19532.a.B2.GE41664 ordered domain, RhoGEF (DH-PH tandem) and UVR8-like 468 1426
NafoA.19532.a.B21.GE42357 ordered domain, RhoGEF (DH-PH tandem) and UVR8-like 468 1426
NafoA.19532.a.B22.GE42358 ordered domain, RhoGEF (DH-PH tandem) and UVR8-like 468 1426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0007100

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANKFDFSHN HFILSLVEKM SNLKEKIAAF ENRSLMASQP PPHHHKEQVI HSSQHQQPPQ TNRSGGLNVP PSAVGHGTPS PSTPSVATNI NHHHTTTPTI SAKTPMSSHH SNGKQLPPTP TDRSSLNIGL SRNINPPPPP NTTPRSATIM TSPPPPPQQP NNSSSNSHHP ITPMESSPPQ NGKSEQNDQD DSTSSNEDED IINNNNNGSM NGGNGGEEEP ESISDNNQQQ HPQQQNTTTL SQQKVHSTDL HQLPKNKPAV PNRFRNNGGG TVASSSGTSS AQFRPKPNPK LPPSLPQPRR AQASVDFGSS RINIGGATGS PLSESTTNNL SGNNLTGGNL VTNLDPNSAI SYSDSASASR ISMRAGNSGM VYASGLSSLP GDKGPQGKFI PYIPAEVEAV MSVTTGSAGV HAHTISRTSM SISSIDLDSE TQQFAAIYKH PTKQQQDAAI VLQKYIRRWE ARKTYKKIQK ARVNRENCAK EILSTEKSYV ESLNILQEVY LKPFKAMAAK EKPIISKEEV QAIFSDIDVI LNVNTTFLND LEERLKTYEH NVQISDIFLT IAPFFKTYSR YCNNFDKANE VLKECKKRED FKEVVVKMDK DSRTSKLGLQ SYLILPIQRI PRYRLLLQDL IKHSGPDHPD YKGLKNALDK ICEVADHLNN TMKIIEATND IIKIQSQFYG DINLVEPHRR FLKEGALQEI ISSDFTKTRD TYIHLFNDIV LFSQKGGKFT LKVQFPLNDL VIFNYVNQDD QIDDLIFKVQ SPSKAIILKA ASKSDKDNWA ITLHNLIKEC KNSKRNKTLN VDEHTIADDS STTFALAMSD NDEITIPPEE CVRRMKEGST MLKYCRNAKP HFRRFVLSND GTELMWGSPN KQSNESIVSL TDVKKICYGQ NTAIFQKYKN PDLEGLSFSL LYRDRTLDIV CKDKQEYHTW VQGVTYLLSH MEDILNQKRI APPTASNEPK FAPPALVHDM ENEKQKFKEQ FERIGDAYTW GQGACGGLGH GNQEDKKEPL VMKDFLYLDV SLVACENSAG AGVMFTGELF TWGKGEKGRL GHSDTNDRIK PTYVKALQGR NIKHVCMGNL HTLCLDDTGT VFAFGDYQYG QCGLEKVDGF TSTPTPLPIC TNGFKVTTIA CGHWHSAIVS NDGQLLTFGR GEDGQLGFGD KSNRATPTRV KGVLDDYKIV DVACGLWHTM ALTEEGYLFT WGNNTYGQCG HNNTDEYLLP AFVETFRNMP VKQIAAGSAH SGCITEGDGA VYMWGNGIYG QIGNNSNTHA LSPMRVKFGK LKAKQLVCGV NHAMTLMEDG SVYAWGAGTY GRLGINSEGD QRVPVRITFF DDKSVRTLGA GGSQSAAVCA HQWVPDKDAT NCMNESCKAK FTFLRRRHHC RYCGGIFCGN CTTKRITLLR FGFDEPVRVC DNCYQILTNA AQTYKR
NT Sequence
atggcaaaca aattcgattt ttctcacaat catttcatcc tttcactcgt tgaaaaaatg tcaaatctca aggagaaaat cgctgccttt gagaaccgca gtctgatggc gtcccagcct cctcctcatc atcataaaga gcaggtgatt cattcctccc aacaccaaca accaccacaa acgaatagaa gtggtggatt gaatgttcct ccgtccgcag taggccatgg tactccttct ccctcaactc catctgttgc aacgaatatt aatcatcacc acaccacaac accaacaata tcggcaaaga ctcctatgtc atcacatcac tcaaacggaa aacaacttcc accaacacca acggatagga gctctctcaa tattggactt tcaagaaaca taaatccacc tcctccaccg aacactactc cccgatcagc aacgatcatg acatcaccac caccaccacc acaacaaccc aataatagta gtagcaattc acatcaccca atcacaccaa tggagtcatc acctccacag aatggaaaat ctgagcaaaa tgatcaagat gattctacat cctccaacga agatgaagat atcataaata acaacaacaa tggatccatg aacggaggaa acggtggtga agaagagcct gaatccattt ctgataacaa tcaacaacaa cacccacaac aacaaaacac aacaacttta tcacaacaaa aagtacattc tacggacctt catcaattac caaaaaacaa acctgctgta ccaaatagat ttagaaacaa tggaggaggt accgtagcaa gttctagtgg cactagctca gctcaattta gacccaaacc aaatcccaaa ttacctccaa gtcttcctca accacgacgt gctcaagcat ctgttgactt tggaagtagt cgtattaata ttggtggagc aacaggatca cctctctctg aaagtaccac gaataatctt tctggcaaca atttgaccgg tggcaatctc gtaaccaatt tagatccaaa ctcagctatt tcgtactctg attctgcatc agcatcaaga atttcaatga gagcaggtaa tagtggaatg gtttatgctt ctggattgtc ttcactacct ggtgacaagg gtccacaagg aaagtttatt ccctatattc cagcagaagt tgaggctgtc atgagtgtta ccactggaag tgctggtgta catgctcaca cgatcagtcg aacaagtatg agcatcagtt ccatcgattt agattctgaa actcaacaat ttgctgccat ttacaaacat cctacaaaac agcaacaaga tgcagccata gttttacaga aatacattag aagatgggaa gccagaaaga cgtataaaaa gattcaaaaa gccagagtga atcgagaaaa ttgtgccaaa gaaattcttt ctaccgagaa gagttatgtg gaatctctca acattcttca agaagtatac ttaaaaccat tcaaagcaat ggctgccaaa gaaaaaccaa tcatcagcaa agaagaagtt caagctattt tttctgacat tgatgttatt ttgaacgtca atacaacctt tttgaatgat ttagaggaaa gacttaaaac ctatgaacac aatgtccaga ttagtgacat tttccttact attgctccat tcttcaagac atactcacga tactgtaaca actttgataa ggccaacgag gttttgaaag aatgtaaaaa acgagaagat ttcaaagagg tggttgtcaa gatggacaag gatagcagaa catccaagtt aggtcttcaa agttatctta tcttacctat ccaacgtatt ccaagatata gacttctttt acaagatttg atcaaacact ctggtcctga tcatcctgat tacaagggat tgaaaaacgc tcttgataag atttgtgaag ttgctgacca tcttaacaac acaatgaaaa tcattgaagc taccaatgac attattaaga ttcaatctca attctatggt gatattaacc ttgtagagcc tcatagaaga tttttaaagg aaggagcgct tcaagaaatt atttcttcag attttacaaa aacaagagat acctatattc atttatttaa tgacattgta ctattctctc agaaaggagg aaaatttaca ctcaaagttc aatttccact caatgacttg gttatattta attatgttaa tcaagatgat caaattgatg atttaatttt caaagtacag tctccaagca aagcaattat tctgaaagca gcctccaaat ctgataaaga taactgggcc atcaccttac acaatcttat caaggaatgt aaaaactcaa agcgaaacaa gacacttaat gtagatgaac atactattgc cgacgattct tcaacaacgt ttgctcttgc tatgagcgat aatgatgaaa ttactattcc tcctgaggaa tgtgttcgaa gaatgaaaga aggatccacc atgctcaagt attgtagaaa tgccaaacct cactttagaa gattcgttct cagtaatgac ggaacagaat taatgtgggg atctccaaac aagcaatcga atgagtccat tgtttcatta acagatgtga agaagatttg ttatggacaa aacacagcca ttttccaaaa gtataagaat cctgatctag aaggtctttc attctcactt ctctatagag atagaacttt ggatatcgta tgtaaggata agcaagaata tcatacctgg gttcagggtg taacatactt actctcacac atggaagaca ttctcaatca gaaacgaatt gcgccaccca ctgcttctaa cgaaccaaaa tttgcgccac ctgcccttgt acatgacatg gagaatgaaa aacaaaaatt caaggaacaa ttcgaaagaa ttggagatgc ctatacatgg ggtcaaggtg cttgtggcgg tcttgggcat ggaaatcaag aagacaagaa agaacctctt gtcatgaaag actttttgta tttggatgta tctttggttg catgcgaaaa ctcggcaggt gctggtgtca tgtttactgg agagttattc acgtggggaa agggtgagaa aggtagattg ggtcatagtg acaccaatga tagaattaaa cctacctatg tgaaagcatt gcaaggaagg aacatcaaac atgtctgtat gggtaattta catacgttgt gtcttgatga cactggtact gtgtttgctt ttggtgatta tcaatatgga caatgtggtc ttgaaaaagt ggatggtttt acttcaacac caactcctct acccatttgt acgaatggtt tcaaggttac tacgattgct tgtgggcatt ggcattcagc tattgtttcc aatgatggtc aattattaac ttttggaaga ggagaagatg gacaacttgg atttggagat aaaagtaata gagctactcc aaccagagtc aaaggagttt tagatgacta taagattgta gatgttgctt gtggactatg gcataccatg gcccttactg aagaaggata tcttttcaca tggggaaata atacctatgg tcaatgcgga cacaataata ctgacgagta tttattacca gcatttgtgg agacgtttag aaatatgcct gttaagcaaa ttgctgctgg tagtgctcac agtggatgta taacagaagg agatggagct gtatatatgt ggggtaatgg tatttatggt caaattggaa acaattcaaa tactcatgca ttgagtccta tgagagtcaa gtttggcaaa cttaaggcta agcaacttgt ttgtggtgtc aaccacgcca tgacattgat ggaagatgga tcagtgtatg cttggggtgc tggtacttat ggtcgacttg gtatcaatag tgaaggcgat caacgagttc ctgttcgaat aacattcttc gatgacaaga gtgttcgtac gcttggtgca ggaggttctc aaagcgcagc tgtgtgtgct caccaatggg ttccagacaa ggatgctacc aattgtatga atgaaagttg caaagctaaa ttcaccttcc ttagaagaag acatcactgc agatactgtg gaggtatttt ctgtggaaat tgtaccacaa agagaatcac attacttcgt tttggttttg atgaaccagt tagagtttgt gacaattgtt atcaaatttt aacaaatgct gctcaaacct ataagagata a
Details for NafoA.19532.a.B2.GE41664
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 109kDa
OBSERVED MW: 109kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHIQ KARVNRENCA KEILSTEKSY VESLNILQEV YLKPFKAMAA KEKPIISKEE VQAIFSDIDV ILNVNTTFLN DLEERLKTYE HNVQISDIFL TIAPFFKTYS RYCNNFDKAN EVLKECKKRE DFKEVVVKMD KDSRTSKLGL QSYLILPIQR IPRYRLLLQD LIKHSGPDHP DYKGLKNALD KICEVADHLN NTMKIIEATN DIIKIQSQFY GDINLVEPHR RFLKEGALQE IISSDFTKTR DTYIHLFNDI VLFSQKGGKF TLKVQFPLND LVIFNYVNQD DQIDDLIFKV QSPSKAIILK AASKSDKDNW AITLHNLIKE CKNSKRNKTL NVDEHTIADD SSTTFALAMS DNDEITIPPE ECVRRMKEGS TMLKYCRNAK PHFRRFVLSN DGTELMWGSP NKQSNESIVS LTDVKKICYG QNTAIFQKYK NPDLEGLSFS LLYRDRTLDI VCKDKQEYHT WVQGVTYLLS HMEDILNQKR IAPPTASNEP KFAPPALVHD MENEKQKFKE QFERIGDAYT WGQGACGGLG HGNQEDKKEP LVMKDFLYLD VSLVACENSA GAGVMFTGEL FTWGKGEKGR LGHSDTNDRI KPTYVKALQG RNIKHVCMGN LHTLCLDDTG TVFAFGDYQY GQCGLEKVDG FTSTPTPLPI CTNGFKVTTI ACGHWHSAIV SNDGQLLTFG RGEDGQLGFG DKSNRATPTR VKGVLDDYKI VDVACGLWHT MALTEEGYLF TWGNNTYGQC GHNNTDEYLL PAFVETFRNM PVKQIAAGSA HSGCITEGDG AVYMWGNGIY GQIGNNSNTH ALSPMRVKFG KLKAKQLVCG VNHAMTLMED GSVYAWGAGT YGRLGINSEG DQRVPVRITF FDDKSVRTLG AGGSQSAAVC AHQWVPDKDA TNCMNESCKA KFTFLRRRHH CRYCGGIFCG NCTTKRITLL RFGFDEPVRV CDNCYQILTN AAQTYKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattcaaa aagccagagt gaatcgagaa aattgtgcca aagaaattct ttctaccgag aagagttatg tggaatctct caacattctt caagaagtat acttaaaacc attcaaagca atggctgcca aagaaaaacc aatcatcagc aaagaagaag ttcaagctat tttttctgac attgatgtta ttttgaacgt caatacaacc tttttgaatg atttagagga aagacttaaa acctatgaac acaatgtcca gattagtgac attttcctta ctattgctcc attcttcaag acatactcac gatactgtaa caactttgat aaggccaacg aggttttgaa agaatgtaaa aaacgagaag atttcaaaga ggtggttgtc aagatggaca aggatagcag aacatccaag ttaggtcttc aaagttatct tatcttacct atccaacgta ttccaagata tagacttctt ttacaagatt tgatcaaaca ctctggtcct gatcatcctg attacaaggg attgaaaaac gctcttgata agatttgtga agttgctgac catcttaaca acacaatgaa aatcattgaa gctaccaatg acattattaa gattcaatct caattctatg gtgatattaa ccttgtagag cctcatagaa gatttttaaa ggaaggagcg cttcaagaaa ttatttcttc agattttaca aaaacaagag atacctatat tcatttattt aatgacattg tactattctc tcagaaagga ggaaaattta cactcaaagt tcaatttcca ctcaatgact tggttatatt taattatgtt aatcaagatg atcaaattga tgatttaatt ttcaaagtac agtctccaag caaagcaatt attctgaaag cagcctccaa atctgataaa gataactggg ccatcacctt acacaatctt atcaaggaat gtaaaaactc aaagcgaaac aagacactta atgtagatga acatactatt gccgacgatt cttcaacaac gtttgctctt gctatgagcg ataatgatga aattactatt cctcctgagg aatgtgttcg aagaatgaaa gaaggatcca ccatgctcaa gtattgtaga aatgccaaac ctcactttag aagattcgtt ctcagtaatg acggaacaga attaatgtgg ggatctccaa acaagcaatc gaatgagtcc attgtttcat taacagatgt gaagaagatt tgttatggac aaaacacagc cattttccaa aagtataaga atcctgatct agaaggtctt tcattctcac ttctctatag agatagaact ttggatatcg tatgtaagga taagcaagaa tatcatacct gggttcaggg tgtaacatac ttactctcac acatggaaga cattctcaat cagaaacgaa ttgcgccacc cactgcttct aacgaaccaa aatttgcgcc acctgccctt gtacatgaca tggagaatga aaaacaaaaa ttcaaggaac aattcgaaag aattggagat gcctatacat ggggtcaagg tgcttgtggc ggtcttgggc atggaaatca agaagacaag aaagaacctc ttgtcatgaa agactttttg tatttggatg tatctttggt tgcatgcgaa aactcggcag gtgctggtgt catgtttact ggagagttat tcacgtgggg aaagggtgag aaaggtagat tgggtcatag tgacaccaat gatagaatta aacctaccta tgtgaaagca ttgcaaggaa ggaacatcaa acatgtctgt atgggtaatt tacatacgtt gtgtcttgat gacactggta ctgtgtttgc ttttggtgat tatcaatatg gacaatgtgg tcttgaaaaa gtggatggtt ttacttcaac accaactcct ctacccattt gtacgaatgg tttcaaggtt actacgattg cttgtgggca ttggcattca gctattgttt ccaatgatgg tcaattatta acttttggaa gaggagaaga tggacaactt ggatttggag ataaaagtaa tagagctact ccaaccagag tcaaaggagt tttagatgac tataagattg tagatgttgc ttgtggacta tggcatacca tggcccttac tgaagaagga tatcttttca catggggaaa taatacctat ggtcaatgcg gacacaataa tactgacgag tatttattac cagcatttgt ggagacgttt agaaatatgc ctgttaagca aattgctgct ggtagtgctc acagtggatg tataacagaa ggagatggag ctgtatatat gtggggtaat ggtatttatg gtcaaattgg aaacaattca aatactcatg cattgagtcc tatgagagtc aagtttggca aacttaaggc taagcaactt gtttgtggtg tcaaccacgc catgacattg atggaagatg gatcagtgta tgcttggggt gctggtactt atggtcgact tggtatcaat agtgaaggcg atcaacgagt tcctgttcga ataacattct tcgatgacaa gagtgttcgt acgcttggtg caggaggttc tcaaagcgca gctgtgtgtg ctcaccaatg ggttccagac aaggatgcta ccaattgtat gaatgaaagt tgcaaagcta aattcacctt ccttagaaga agacatcact gcagatactg tggaggtatt ttctgtggaa attgtaccac aaagagaatc acattacttc gttttggttt tgatgaacca gttagagttt gtgacaattg ttatcaaatt ttaacaaatg ctgctcaaac ctataagaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NafoA.19532.a.B21.GE42357
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 109kDa
OBSERVED MW: 109kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHIQ KARVNRENCA KEILSTEKSY VESLNILQEV YLKPFKAMAA KEKPIISKEE VQAIFSDIDV ILNVNTTFLN DLEERLKTYE HNVQISDIFL TIAPFFKTYS RYCNNFDKAN EVLKECKKRE DFKEVVVKMD KDSRTSKLGL QSYLILPIQR IPRYRLLLQD LIKHSGPDHP DYKGLKNALD KICEVADHLN NTMKIIEATN DIIKIQSQFY GDINLVEPHR RFLKEGALQE IISSDFTKTR DTYIHLFNDI VLFSQKGGKF TLKVQFPLND LVIFNYVNQD DQIDDLIFKV QSPSKAIILK AASKSDKDNW AITLHNLIKE CKNSKRNKTL NVDEHTIADD SSTTFALAMS DNDEITIPPE ECVRRMKEGS TMLKYCRNAK PHFRRFVLSN DGTELMWGSP NKQSNESIVS LTDVKKICYG QNTAIFQKYK NPDLEGLSFS LLYRDRTLDI VCKDKQEYHT WVQGVTYLLS HMEDILNQKR IAPPTASNEP KFAPPALVHD MENEKQKFKE QFERIGDAYT WGQGACGGLG HGNQEDKKEP LVMKDFLYLD VSLVACENSA GAGVMFTGEL FTWGKGEKGR LGHSDTNDRI KPTYVKALQG RNIKHVCMGN LHTLCLDDTG TVFAFGDYQY GQCGLEKVDG FTSTPTPLPI CTNGFKVTTI ACGHWHSAIV SNDGQLLTFG RGEDGQLGFG DKSNRATPTR VKGVLDDYKI VDVACGLWHT MALTEEGYLF TWGNNTYGQC GHNNTDEYLL PAFVETFRNM PVKQIAAGSA HSGCITEGDG AVYMWGNGIY GQIGNNSNTH ALSPMRVKFG KLKAKQLVCG VNHAMTLMED GSVYAWGAGT YGRLGINSEG DQRVPVRITF FDDKSVRTLG AGGSQSAAVC AHQWVPDKDA TNCMNESCKA KFTFLRRRHH CRYCGGIFCG NCTTKRITLL RFGFDEPVRV CDNCYQILTN AAQTYKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattcaaa aagccagagt gaatcgagaa aattgtgcca aagaaattct ttctaccgag aagagttatg tggaatctct caacattctt caagaagtat acttaaaacc attcaaagca atggctgcca aagaaaaacc aatcatcagc aaagaagaag ttcaagctat tttttctgac attgatgtta ttttgaacgt caatacaacc tttttgaatg atttagagga aagacttaaa acctatgaac acaatgtcca gattagtgac attttcctta ctattgctcc attcttcaag acatactcac gatactgtaa caactttgat aaggccaacg aggttttgaa agaatgtaaa aaacgagaag atttcaaaga ggtggttgtc aagatggaca aggatagcag aacatccaag ttaggtcttc aaagttatct tatcttacct atccaacgta ttccaagata tagacttctt ttacaagatt tgatcaaaca ctctggtcct gatcatcctg attacaaggg attgaaaaac gctcttgata agatttgtga agttgctgac catcttaaca acacaatgaa aatcattgaa gctaccaatg acattattaa gattcaatct caattctatg gtgatattaa ccttgtagag cctcatagaa gatttttaaa ggaaggagcg cttcaagaaa ttatttcttc agattttaca aaaacaagag atacctatat tcatttattt aatgacattg tactattctc tcagaaagga ggaaaattta cactcaaagt tcaatttcca ctcaatgact tggttatatt taattatgtt aatcaagatg atcaaattga tgatttaatt ttcaaagtac agtctccaag caaagcaatt attctgaaag cagcctccaa atctgataaa gataactggg ccatcacctt acacaatctt atcaaggaat gtaaaaactc aaagcgaaac aagacactta atgtagatga acatactatt gccgacgatt cttcaacaac gtttgctctt gctatgagcg ataatgatga aattactatt cctcctgagg aatgtgttcg aagaatgaaa gaaggatcca ccatgctcaa gtattgtaga aatgccaaac ctcactttag aagattcgtt ctcagtaatg acggaacaga attaatgtgg ggatctccaa acaagcaatc gaatgagtcc attgtttcat taacagatgt gaagaagatt tgttatggac aaaacacagc cattttccaa aagtataaga atcctgatct agaaggtctt tcattctcac ttctctatag agatagaact ttggatatcg tatgtaagga taagcaagaa tatcatacct gggttcaggg tgtaacatac ttactctcac acatggaaga cattctcaat cagaaacgaa ttgcgccacc cactgcttct aacgaaccaa aatttgcgcc acctgccctt gtacatgaca tggagaatga aaaacaaaaa ttcaaggaac aattcgaaag aattggagat gcctatacat ggggtcaagg tgcttgtggc ggtcttgggc atggaaatca agaagacaag aaagaacctc ttgtcatgaa agactttttg tatttggatg tatctttggt tgcatgcgaa aactcggcag gtgctggtgt catgtttact ggagagttat tcacgtgggg aaagggtgag aaaggtagat tgggtcatag tgacaccaat gatagaatta aacctaccta tgtgaaagca ttgcaaggaa ggaacatcaa acatgtctgt atgggtaatt tacatacgtt gtgtcttgat gacactggta ctgtgtttgc ttttggtgat tatcaatatg gacaatgtgg tcttgaaaaa gtggatggtt ttacttcaac accaactcct ctacccattt gtacgaatgg tttcaaggtt actacgattg cttgtgggca ttggcattca gctattgttt ccaatgatgg tcaattatta acttttggaa gaggagaaga tggacaactt ggatttggag ataaaagtaa tagagctact ccaaccagag tcaaaggagt tttagatgac tataagattg tagatgttgc ttgtggacta tggcatacca tggcccttac tgaagaagga tatcttttca catggggaaa taatacctat ggtcaatgcg gacacaataa tactgacgag tatttattac cagcatttgt ggagacgttt agaaatatgc ctgttaagca aattgctgct ggtagtgctc acagtggatg tataacagaa ggagatggag ctgtatatat gtggggtaat ggtatttatg gtcaaattgg aaacaattca aatactcatg cattgagtcc tatgagagtc aagtttggca aacttaaggc taagcaactt gtttgtggtg tcaaccacgc catgacattg atggaagatg gatcagtgta tgcttggggt gctggtactt atggtcgact tggtatcaat agtgaaggcg atcaacgagt tcctgttcga ataacattct tcgatgacaa gagtgttcgt acgcttggtg caggaggttc tcaaagcgca gctgtgtgtg ctcaccaatg ggttccagac aaggatgcta ccaattgtat gaatgaaagt tgcaaagcta aattcacctt ccttagaaga agacatcact gcagatactg tggaggtatt ttctgtggaa attgtaccac aaagagaatc acattacttc gttttggttt tgatgaacca gttagagttt gtgacaattg ttatcaaatt ttaacaaatg ctgctcaaac ctataagaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NafoA.19532.a.B22.GE42358
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 109kDa
OBSERVED MW: 109kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHIQ KARVNRENCA KEILSTEKSY VESLNILQEV YLKPFKAMAA KEKPIISKEE VQAIFSDIDV ILNVNTTFLN DLEERLKTYE HNVQISDIFL TIAPFFKTYS RYCNNFDKAN EVLKECKKRE DFKEVVVKMD KDSRTSKLGL QSYLILPIQR IPRYRLLLQD LIKHSGPDHP DYKGLKNALD KICEVADHLN NTMKIIEATN DIIKIQSQFY GDINLVEPHR RFLKEGALQE IISSDFTKTR DTYIHLFNDI VLFSQKGGKF TLKVQFPLND LVIFNYVNQD DQIDDLIFKV QSPSKAIILK AASKSDKDNW AITLHNLIKE CKNSKRNKTL NVDEHTIADD SSTTFALAMS DNDEITIPPE ECVRRMKEGS TMLKYCRNAK PHFRRFVLSN DGTELMWGSP NKQSNESIVS LTDVKKICYG QNTAIFQKYK NPDLEGLSFS LLYRDRTLDI VCKDKQEYHT WVQGVTYLLS HMEDILNQKR IAPPTASNEP KFAPPALVHD MENEKQKFKE QFERIGDAYT WGQGACGGLG HGNQEDKKEP LVMKDFLYLD VSLVACENSA GAGVMFTGEL FTWGKGEKGR LGHSDTNDRI KPTYVKALQG RNIKHVCMGN LHTLCLDDTG TVFAFGDYQY GQCGLEKVDG FTSTPTPLPI CTNGFKVTTI ACGHWHSAIV SNDGQLLTFG RGEDGQLGFG DKSNRATPTR VKGVLDDYKI VDVACGLWHT MALTEEGYLF TWGNNTYGQC GHNNTDEYLL PAFVETFRNM PVKQIAAGSA HSGCITEGDG AVYMWGNGIY GQIGNNSNTH ALSPMRVKFG KLKAKQLVCG VNHAMTLMED GSVYAWGAGT YGRLGINSEG DQRVPVRITF FDDKSVRTLG AGGSQSAAVC AHQWVPDKDA TNCMNESCKA KFTFLRRRHH CRYCGGIFCG NCTTKRITLL RFGFDEPVRV CDNCYQILTN AAQTYKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattcaaa aagccagagt gaatcgagaa aattgtgcca aagaaattct ttctaccgag aagagttatg tggaatctct caacattctt caagaagtat acttaaaacc attcaaagca atggctgcca aagaaaaacc aatcatcagc aaagaagaag ttcaagctat tttttctgac attgatgtta ttttgaacgt caatacaacc tttttgaatg atttagagga aagacttaaa acctatgaac acaatgtcca gattagtgac attttcctta ctattgctcc attcttcaag acatactcac gatactgtaa caactttgat aaggccaacg aggttttgaa agaatgtaaa aaacgagaag atttcaaaga ggtggttgtc aagatggaca aggatagcag aacatccaag ttaggtcttc aaagttatct tatcttacct atccaacgta ttccaagata tagacttctt ttacaagatt tgatcaaaca ctctggtcct gatcatcctg attacaaggg attgaaaaac gctcttgata agatttgtga agttgctgac catcttaaca acacaatgaa aatcattgaa gctaccaatg acattattaa gattcaatct caattctatg gtgatattaa ccttgtagag cctcatagaa gatttttaaa ggaaggagcg cttcaagaaa ttatttcttc agattttaca aaaacaagag atacctatat tcatttattt aatgacattg tactattctc tcagaaagga ggaaaattta cactcaaagt tcaatttcca ctcaatgact tggttatatt taattatgtt aatcaagatg atcaaattga tgatttaatt ttcaaagtac agtctccaag caaagcaatt attctgaaag cagcctccaa atctgataaa gataactggg ccatcacctt acacaatctt atcaaggaat gtaaaaactc aaagcgaaac aagacactta atgtagatga acatactatt gccgacgatt cttcaacaac gtttgctctt gctatgagcg ataatgatga aattactatt cctcctgagg aatgtgttcg aagaatgaaa gaaggatcca ccatgctcaa gtattgtaga aatgccaaac ctcactttag aagattcgtt ctcagtaatg acggaacaga attaatgtgg ggatctccaa acaagcaatc gaatgagtcc attgtttcat taacagatgt gaagaagatt tgttatggac aaaacacagc cattttccaa aagtataaga atcctgatct agaaggtctt tcattctcac ttctctatag agatagaact ttggatatcg tatgtaagga taagcaagaa tatcatacct gggttcaggg tgtaacatac ttactctcac acatggaaga cattctcaat cagaaacgaa ttgcgccacc cactgcttct aacgaaccaa aatttgcgcc acctgccctt gtacatgaca tggagaatga aaaacaaaaa ttcaaggaac aattcgaaag aattggagat gcctatacat ggggtcaagg tgcttgtggc ggtcttgggc atggaaatca agaagacaag aaagaacctc ttgtcatgaa agactttttg tatttggatg tatctttggt tgcatgcgaa aactcggcag gtgctggtgt catgtttact ggagagttat tcacgtgggg aaagggtgag aaaggtagat tgggtcatag tgacaccaat gatagaatta aacctaccta tgtgaaagca ttgcaaggaa ggaacatcaa acatgtctgt atgggtaatt tacatacgtt gtgtcttgat gacactggta ctgtgtttgc ttttggtgat tatcaatatg gacaatgtgg tcttgaaaaa gtggatggtt ttacttcaac accaactcct ctacccattt gtacgaatgg tttcaaggtt actacgattg cttgtgggca ttggcattca gctattgttt ccaatgatgg tcaattatta acttttggaa gaggagaaga tggacaactt ggatttggag ataaaagtaa tagagctact ccaaccagag tcaaaggagt tttagatgac tataagattg tagatgttgc ttgtggacta tggcatacca tggcccttac tgaagaagga tatcttttca catggggaaa taatacctat ggtcaatgcg gacacaataa tactgacgag tatttattac cagcatttgt ggagacgttt agaaatatgc ctgttaagca aattgctgct ggtagtgctc acagtggatg tataacagaa ggagatggag ctgtatatat gtggggtaat ggtatttatg gtcaaattgg aaacaattca aatactcatg cattgagtcc tatgagagtc aagtttggca aacttaaggc taagcaactt gtttgtggtg tcaaccacgc catgacattg atggaagatg gatcagtgta tgcttggggt gctggtactt atggtcgact tggtatcaat agtgaaggcg atcaacgagt tcctgttcga ataacattct tcgatgacaa gagtgttcgt acgcttggtg caggaggttc tcaaagcgca gctgtgtgtg ctcaccaatg ggttccagac aaggatgcta ccaattgtat gaatgaaagt tgcaaagcta aattcacctt ccttagaaga agacatcact gcagatactg tggaggtatt ttctgtggaa attgtaccac aaagagaatc acattacttc gttttggttt tgatgaacca gttagagttt gtgacaattg ttatcaaatt ttaacaaatg ctgctcaaac ctataagaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat