NafoA.19531.b

regulator of heterotrimeric G proteins RGS2

CENTER ID: NafoA.19531.b
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.19531.b.B2.GE41663 RGS domain 1262 1419
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.19531.b.B2.PW38363 RGS domain 1262 1419

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0023670

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLVSTDETLM IVIRKDGLLI KDEFEEDYLI SSNDANGQVF DELILNTDGS LELKGPGFNL AIFDPCIGGT PPYRLILGDT HFTIYDSLMN ICQTSLDDEL PISVNVKYGG PQCSEPICYG INGGNSSACE GHGSCSEPNS CSCFTGYYGE KCQYFRCNGI PSDSPLACSS NGNCTALNQC DCFEGFFGSQ CQNFQCFNVS NINETVCSGH GTCVKTNQCN CTPSNQYFGE QCEITSCFSI MSNSTNPKPC SGHGKCFAFN NCSCDSSDYF GSQCDQFNCF NIHKDSSSYS LEQRYKDTKI FNLSLFNIST AGRYRFVMNA TLFNNHSSYR YWKTRMVEFY VTFIGDPTLV LYGLPQALVA NVEQSFQVIL SGVKFPQRCE EIPNHFLGKN KVMNNASQLV SFSLELLDFS NNETQCSTSG KTFASGTLLE EGSYTTSMLS PVIVLQCTLV SKGASMSPLS LKVVLTNDMD TADTLQFSYD IPLLSFNVPS LRNNVTITPM NGTALDTLFS VSLTDLDELK NIPSPLSPLE FAFGFVWKDS QTSKNARLTP FSLQYENVTF ALPFTFYQGS NLKELLDIVV FMRDAFSNEY PQIIGQVEIY PNTNVNNLAE KVKNNKILAL AATYDRSILS SQTSSNASLE ITLYALQQLN LSALNKDNSE SLQSLTLLSE QTLIEHRIAS AITDKLDQFL SEMKQVYTNE KMNLGFVQNK ADKDLQSTLE LVSNLMKNGF FKDEIVELCQ QISTIAQIGE NPTLLRSDSS NIPKFLFSEL VNISVMSFIP DVSLQNHSKN TFLQDVEFNI PNILSKYHGF SQEIGIKLVS FEEGVKTLQH DQNQLISPVK DFSFSRNMKD LDLRDLSEPV VLSFTIQNQT LDLNISQLSC RYWNETTESW KTNGCVLLKV VNEPRQVFCS CNHTTMFSVF LHHDNSSIME EPIRQEFLAT LFIGQITVGA VYFLVSLVIL IALFIFRSEQ PVKSRLFTPY VGMISLMIQS ILISMTQKGV LLSTVQENIS TQSSVAKWEG PLQSANILGN VVMIISNTFS ITAIVFYLLQ VTRFQLLKYF YYKMSRRNHE SLRKTKEVPK LISISMTPKM IKFMISKKFS SLLLVICLGL NLAFWTLFVI LQQTDQEGTQ VMSIATQHTD EKPKDKNGEV VKKVTQWLQV KPLRDWFATL DAPLYFRVEM ILFVLCYVFL ICNQLTGLYT LMNKESSSQQ VMVLDVISFV FEILYVAMYI MVFGGFALLV FLVNKLKALS KQMKIGQEPQ QNQTEFQALL DSKEGRELLE EFCKKEFSLE NMLLYKELMH FKAQQSLAAI HSFMRNIYTN YINAGAPIEV NIPSECRKEF MRVFQELNAT SRELNDIKNS KQSPSQEISS FEQCMNHLTE EILMNLGDTF SRLVATPQYT QFLSVMKNKK DMMKQVNLL
NT Sequence
atgcttgttt ctacagatga gacgctcatg atcgtcatac gaaaagatgg ccttttaatc aaagatgaat ttgaagaaga ttatttgata agttcgaatg acgcaaatgg tcaagttttt gacgaactta ttctcaatac agatggatcg cttgaactga aaggaccagg gtttaatctc gccatttttg atccatgtat aggaggaaca cctccctatc ggttgattct aggagataca cattttacca tctatgattc gttgatgaac atatgtcaaa caagtttgga tgatgaactc cccataagtg tgaatgtgaa atatggtggt cctcaatgtt cagaacccat ctgctatgga attaatggag gaaactcaag tgcttgcgaa ggacatggta gttgctcaga accaaactct tgctcgtgtt tcactggata ctatggagag aagtgtcaat attttagatg caatggaatt ccttctgact ccccattggc ctgttcatcg aatggcaatt gcacagctct aaatcagtgt gactgttttg agggattctt tggctcccaa tgccaaaact ttcaatgttt taatgtttcc aacatcaatg agactgtatg ttctggacat ggcacctgtg taaaaacaaa tcaatgcaat tgcactccat ccaatcagta ttttggagaa cagtgtgaaa taacatcgtg tttttcaatc atgtccaatt ccaccaaccc aaaaccatgt tctggccatg gcaagtgttt tgcatttaac aattgttcct gtgactcttc tgactatttt ggttcacaat gtgatcagtt caattgcttt aacattcata aggacagtag ctcgtactct ctcgaacaaa gatacaaaga tacaaagata ttcaatctct ccttgtttaa catttcaact gctgggaggt atagatttgt catgaatgca actctcttca acaaccattc atcttataga tactggaaga caagaatggt cgaattttat gtcaccttta taggagatcc aacactagtc ctgtatggtt tacctcaagc attggtcgcg aatgtcgaac aatcttttca ggtcatactt tcaggtgtga aatttccgca aagatgtgaa gaaattccca accatttcct tggaaagaac aaggtaatga ataatgcctc acagttggtt tcattctcat tagaactttt agacttttcg aataatgaaa ctcaatgttc gacctctgga aagacatttg cttctggaac tcttcttgaa gaaggatctt acacaacttc gatgctttct ccggtcattg tattgcaatg taccttggtg tctaaaggtg catccatgtc accactctct ctgaaggttg tgctcaccaa cgatatggat acggcagata ctctacagtt cagttatgac ataccattgc taagttttaa cgttccatcg ttaagaaaca atgtgacgat cactcccatg aacggaactg cccttgacac tcttttttct gtttctctga cggatttgga tgaattgaaa aacattcctt ccccactaag tcccttagaa tttgctttcg gatttgtttg gaaggattca cagacttcaa agaatgccag attgacacct ttttcacttc agtatgaaaa tgtcacattc gctctaccat ttactttcta tcaaggatca aaccttaaag aactattaga tattgtagta tttatgcgag atgctttctc caatgaatac cctcaaatta ttggtcaagt ggagatttac ccaaacacca acgtcaacaa tttggctgag aaagtgaaaa ataataagat tttggcattg gctgccacat atgatcgttc aattctctcc tctcaaacat ccagcaatgc ttctcttgaa atcacccttt acgctcttca acagttaaac ctctccgctc ttaataagga taattcagaa tccttacaat cactaacact tctttcagaa caaaccttaa tagagcatcg cattgcttct gcaatcactg ataagcttga tcaattccta agtgaaatga aacaagtcta tacgaatgaa aagatgaatt tgggttttgt tcaaaacaag gcagataagg atttacaatc tactctcgaa ctcgtgtcca atttaatgaa gaatggattt ttcaaggatg aaattgtaga actttgtcaa caaatttcaa ccattgcaca aattggagag aacccaacat tgctccgttc tgattcttca aacataccaa agtttttatt ctctgaactt gtgaacattt cagtgatgag cttcataccg gatgtttctc ttcaaaatca ttcaaagaat acattcctgc aagatgtaga gttcaatatt ccaaatattc tctcaaaata tcatggtttc tcccaggaaa ttggcatcaa gttggtctcc tttgaagagg gagtgaaaac tctccaacac gaccaaaatc aactcatttc accagtgaaa gatttctcct tctcaaggaa tatgaaagat cttgatttac gggatttgtc agagccagta gtgttatcct ttaccattca aaatcaaaca ttggatctca atatttcaca actgtcatgt cgatattgga atgaaacgac cgaatcatgg aaaacgaatg gatgcgtgct gctgaaggtg gtgaatgaac ctcgtcaagt attttgctcg tgcaatcaca cgaccatgtt ctctgtgttc ttgcatcatg acaattcctc gattatggaa gaacctattc gacaagaatt cttggcgaca ttatttattg gacaaataac agtgggagct gtgtatttcc ttgtttcatt agtaatactg attgcactat tcatctttcg atcggaacaa cctgtcaaga gtcgattatt cactccctat gttggaatga tcagcttgat gattcaaagt attttaattt ctatgactca gaaaggagtt ctattgtcaa ccgtgcaaga gaacatttca actcaatcaa gtgttgcaaa gtgggagggc ccattacaaa gcgccaatat tcttggtaat gtagtcatga ttatttcaaa tacttttagc attactgcca ttgtgtttta tttgcttcaa gtgactcgat tccaattatt gaaatacttc tattacaaaa tgagtaggag aaatcatgaa agcttgcgaa agacaaagga ggtaccaaaa ctaataagta tttcaatgac accaaagatg atcaagttca tgatatccaa aaagttttct tcactcttgt tagtgatttg tttgggtttg aatttggctt tttggacttt atttgtgatt ttgcaacaaa cagatcaaga aggtacacaa gtgatgtcca ttgcgaccca acacacggat gaaaagccta aagataagaa tggtgaggta gtgaaaaagg tgacacaatg gcttcaagtg aaacctcttc gagattggtt tgcaacattg gatgctcctc tctatttccg agtggaaatg attttatttg tgttgtgtta cgtcttttta atttgcaacc aactgacagg tctttatacc ttaatgaaca aggaaagttc ctcacaacaa gtcatggtct tagatgtgat atcattcgta tttgaaattt tatatgtcgc aatgtatatc atggtctttg gtggctttgc attgcttgtt ttccttgtta acaaattgaa agctctttcc aaacaaatga agataggtca agagccacaa caaaatcaaa cggaatttca agccctcttg gacagcaagg aaggaagaga acttcttgag gaattttgca aaaaagaatt ctcactggag aacatgttgt tgtacaagga actaatgcat tttaaagctc aacaatcact cgctgccatt cattctttca tgagaaacat ttatacgaat tacattaatg caggagcccc aattgaggtg aatattccat ccgaatgcag aaaagagttt atgagggttt tccaagagct gaatgccact tcacgagaat tgaatgacat caagaattcg aaacagtctc catctcaaga aatctcctca tttgaacaat gcatgaacca tctcactgaa gaaattctaa tgaatttagg agataccttc tcgagattgg tcgcgacgcc gcaatacacg caatttctgt ctgtgatgaa aaacaagaaa gacatgatga aacaagtaaa tcttttgtaa
Details for NafoA.19531.b.B2.GE41663
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 19kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN QTEFQALLDS KEGRELLEEF CKKEFSLENM LLYKELMHFK AQQSLAAIHS FMRNIYTNYI NAGAPIEVNI PSECRKEFMR VFQELNATSR ELNDIKNSKQ SPSQEISSFE QCMNHLTEEI LMNLGDTFSR LVATPQYTQF LSVMKNKKDM MKQVNLL
Validated NT Sequence
atcttactca cttacaaaag atttacttgt ttcatcatgt ctttcttgtt tttcatcaca gacagaaatt gcgtgtattg cggcgtcgcg accaatctcg agaaggtatc tcctaaattc attagaattt cttcagtgag atggttcatg cattgttcaa atgaggagat ttcttgagat ggagactgtt tcgaattctt gatgtcattc aattctcgtg aagtggcatt cagctcttgg aaaaccctca taaactcttt tctgcattcg gatggaatat tcacctcaat tggggctcct gcattaatgt aattcgtata aatgtttctc atgaaagaat gaatggcagc gagtgattgt tgagctttaa aatgcattag ttccttgtac aacaacatgt tctccagtga gaattctttt ttgcaaaatt cctcaagaag ttctcttcct tccttgctgt ccaagagggc ttgaaattcc gtttgattca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHNQ TEFQALLDSK EGRELLEEFC KKEFSLENML LYKELMHFKA QQSLAAIHSF MRNIYTNYIN AGAPIEVNIP SECRKEFMRV FQELNATSRE LNDIKNSKQS PSQEISSFEQ CMNHLTEEIL MNLGDTFSRL VATPQYTQFL SVMKNKKDMM KQVNLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatcaaa cggaatttca agccctcttg gacagcaagg aaggaagaga acttcttgag gaattttgca aaaaagaatt ctcactggag aacatgttgt tgtacaagga actaatgcat tttaaagctc aacaatcact cgctgccatt cattctttca tgagaaacat ttatacgaat tacattaatg caggagcccc aattgaggtg aatattccat ccgaatgcag aaaagagttt atgagggttt tccaagagct gaatgccact tcacgagaat tgaatgacat caagaattcg aaacagtctc catctcaaga aatctcctca tttgaacaat gcatgaacca tctcactgaa gaaattctaa tgaatttagg agataccttc tcgagattgg tcgcgacgcc gcaatacacg caatttctgt ctgtgatgaa aaacaagaaa gacatgatga aacaagtaaa tcttttgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for NafoA.19531.b.B2.PW38363
PURIFICATION DATe: 10/2/2017
CONCENTRATION: 11.65mg/ml
OBSERVED MW: 20kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
IMAC->SEC Protein began to crash during concentration
Validated AA Sequence
MAHHHHHHMN QTEFQALLDS KEGRELLEEF CKKEFSLENM LLYKELMHFK AQQSLAAIHS FMRNIYTNYI NAGAPIEVNI PSECRKEFMR VFQELNATSR ELNDIKNSKQ SPSQEISSFE QCMNHLTEEI LMNLGDTFSR LVATPQYTQF LSVMKNKKDM MKQVNLL
Validated NT Sequence
atcttactca cttacaaaag atttacttgt ttcatcatgt ctttcttgtt tttcatcaca gacagaaatt gcgtgtattg cggcgtcgcg accaatctcg agaaggtatc tcctaaattc attagaattt cttcagtgag atggttcatg cattgttcaa atgaggagat ttcttgagat ggagactgtt tcgaattctt gatgtcattc aattctcgtg aagtggcatt cagctcttgg aaaaccctca taaactcttt tctgcattcg gatggaatat tcacctcaat tggggctcct gcattaatgt aattcgtata aatgtttctc atgaaagaat gaatggcagc gagtgattgt tgagctttaa aatgcattag ttccttgtac aacaacatgt tctccagtga gaattctttt ttgcaaaatt cctcaagaag ttctcttcct tccttgctgt ccaagagggc ttgaaattcc gtttgattca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHNQ TEFQALLDSK EGRELLEEFC KKEFSLENML LYKELMHFKA QQSLAAIHSF MRNIYTNYIN AGAPIEVNIP SECRKEFMRV FQELNATSRE LNDIKNSKQS PSQEISSFEQ CMNHLTEEIL MNLGDTFSRL VATPQYTQFL SVMKNKKDMM KQVNLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAATCAAA CGGAATTTCA AGCCCTCTTG GACAGCAAGG AAGGAAGAGA ACTTCTTGAG GAATTTTGCA AAAAAGAATT CTCACTGGAG AACATGTTGT TGTACAAGGA ACTAATGCAT TTTAAAGCTC AACAATCACT CGCTGCCATT CATTCTTTCA TGAGAAACAT TTATACGAAT TACATTAATG CAGGAGCCCC AATTGAGGTG AATATTCCAT CCGAATGCAG AAAAGAGTTT ATGAGGGTTT TCCAAGAGCT GAATGCCACT TCACGAGAAT TGAATGACAT CAAGAATTCG AAACAGTCTC CATCTCAAGA AATCTCCTCA TTTGAACAAT GCATGAACCA TCTCACTGAA GAAATTCTAA TGAATTTAGG AGATACCTTC TCGAGATTGG TCGCGACGCC GCAATACACG CAATTTCTGT CTGTGATGAA AAACAAGAAA GACATGATGA AACAAGTAAA TCTTTTGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT