NafoA.19531.a

regulator of heterotrimeric G proteins RGS4

CENTER ID: NafoA.19531.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NafoA.19531.a.B2.GE41662 RGS domain 2293 2459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0094010

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRSKIYLAV RIFLIIAGLV MFGIGVTNFV LEVTQESYSI TRDGPLAFVI TQHLTAICSL VLVVQIGAGN TASVKPHANK ESLLQVCAAA LGISIGLCLL GCIPMYLVGA YLINIYRDKS SSPGTDVKVS SHDLGSTSTT ANYVLINARD NDDEDEYDDD DALLLTHNNN EIALKPNSIF VLAVNTCFGI VSTDVNNVCG GHGICQSTDS CYCLDICSKN SLRNNAGAVS LAQSNDLIST NGRFKASMQV DGNFVIYDLQ SNQAIWSTGT NGQGSQPYSL VLDSSGGLRV YGNGAVISTL VQSCSALPAT LGSGSVCSGH GSCNSPNTCS CQSGYSGSNC ETYYCNGILY NSTSVCNSRG TCSASETCVC NANYGGTYCQ IPKCFGILQT ATGTVCSGHG TCNDVDTCAC NTNWGGPSCS FPMCFGILQN ETNSVCHGHG SCNDVDTCAC VAGWGGSQCS MPTCNNILQN QTGIVCGGHG TCDGPDTCHC NSNFTGPFCQ YPFCYGIPSN ASTAVCSGHG VCTGVDTCVC NSNWAGSDCS MPKCFGVLQN STTVCSGHGT CNDANVCACN SNWDGPDCSM PKCFGILQNM TSSVCSGHGS CNDVDTCLCN PNWSGSNCSI PMCFGILQNQ TSEVCNGRGS CNGVDTCVCN SNWGGSNCSI PKCFGILQNM TGSVCSGHGS CQNVDTCVCD LNWGGPSCSL PTCFGILQNE TSSVCNGHGS CNAVDTCVCN LNYGGPSCAL PKCFGVLQNM TSTVCSGHGS CIDVNTCSCL TGWVGLNCSM PTCFGILQNS TSTVCSGHGS CVNLDTCSCM AGWGGPSCAL PKCFGILQNE TNSVCHGHGS CNDVDTCACV AGWGGSQCSM PTCNNILQNQ TGIVCGGHGT CDAPNVCRCN PGWSGDFCFM PSCFGILQNI TSSICSGHGI CTNVDTCLCH TNWTGLNCSI PTCFGFLQNS TNVCSGHGSC TNVDTCVCDS NWGGPSCALP KCFGILQNVT SSVCSGHGSC NAVDTCFVTD KELVTQPMFV DVIQTFLVNG ANIPNVTTLL PMKLQQFVVD MELVCHPTTV LAYPHGLVQI VQSPLALMNC PTRPMCVVEK EVVHLSIIAT AIQIILETNV SSRLVMELLP TTLSFAVTMD NVALLTIVLA PQVMMDPCVK IIDVMEFLLT QPILVVEKVS AFLPTSASGN CSDYNNCTCQ SGFVSMDCSV NINPYVISLS STSMQIDTSS NMTSVEVQMN IPTDVNPLMT SFYWYCLNCQ GISNSSVFML GSNQQKLTIA IRELKAPGSY LFHANATTLN PFPFPTRISN TEVFNLTLLV PYVPNLSVPS LGVFGLPSAF VSTRNGGSGQ TISAVISNIV LNQTNIEKFL GCKANCTTSL TYSWQLTSTS SSSLEASSTG SISAFTGGNN LMILQPPIIV SATLRNLVSN SKIGFALALF NNQMLNISSR VDIPVLPPVA PPTNSSLPVT NNTNAVISSL VQVVPAEGVA LTDTFTINIT AWKAPRELLP LRYAVGFYDK TKGKDVRFTD FSLNLTITVY LPFIQNKARR RNVHAALSQV DLVVYVMDAL GNVDSQSSNV TVTVAPFNGT STELLQRINQ LSGTSLMVAS FDQSYTALSS TSGGNSSAVI YELVKNIVID PKNPSVALSS FESLTSNSDS INDNIVSAVT TKLSSFVDGV KNTYSDEKKL YGFVKSKLSD QDVTSTISVS SNLLSSGVSS DKTRNVTENI SSMVLLGEVA KVIDQSSSAY LPTVSYSTSL INITISSFKL NSGISINQTL SSGSNSVELS ISQILTQYNA FSKECGVSMI EYAKNTNYDN YTSNNVITSV VNDFKFIQEQ TPLILNNLNQ PIILTFKLDS SSLQGKNISN STFTCRYWDE EGRRWSNNGC FLHRVDIPSK TIECACYHTT MFTTFLEQKT TVNMLEVKDQ VAALYFSQIV FGCIYTAIAI FLLVSLIVLR KEQPVTSRLL TPYVGLIALI TESILIYIIQ RGVLVNQLLG ASTQMWETGD TAANVIANIV TIFVNTLNLS AILCYVLQVF RFQLMKFLYQ QMSNSRGNEK NSERILKALK YSTSAALFYT IIAIFAAFNV CYWTLWVILV RTGAISSPTY SYIVSISYLV CILTFSIVIC TVAIFDWILS SKKEVEVKNS RRRPTNNFSQ VNSIALASDS GENLKDPKKK NLITLPTTVM NWFISLDQPL YFRMEMVIYM VCFIFQILNL IIGSSSLSFR FDSNESYKKA LTLDGVSFIF EVFYVVSYLF VFGGFALIVA LIYKFKRSKK KQEQLQKRKE EQKKNQEELI NKEMHVLLEH EEGYKLFEAF CQKEFSLENL YLYSDLKANP EITQGQQLGG IVKFMSFLYN TYVKTGATHE VNIPSKCRNS FLYLYKNVLE SRDQLISTDL KQSADNYEGD IELVDKDIKF ATKNNPIPRQ EIDAETVSNC FEFLLRQVML NIGDTFSRFV FTPEYKSFQK SLEMQQTIMD KVGVVYGI
NT Sequence
atgaaaagaa gcaaaatcta tttggcggtg agaatatttc taattattgc tgggctggtc atgttcggaa ttggcgtcac taactttgtt ctggaagtta ctcaagaaag ttattccatc acacgtgatg gtccacttgc ttttgtcatt acgcaacact tgacagcaat ttgcagttta gttctcgttg ttcaaattgg agctggaaac accgcttcag tgaaacccca cgcaaataag gaaagtttat tacaagtatg cgctgcagct ttgggaatat ccattggtct ttgtttactt ggttgcatac ccatgtacct tgtgggagca tatttaatta acatttatag agataaatca tcatcgccag gcacagatgt gaaagtgtca tctcatgatc tcggaagcac gagtacgaca gctaattatg tcttgatcaa tgcacgtgac aatgacgatg aagatgaata tgatgacgat gatgctctct tgttgactca taataataat gaaattgcac tcaaaccaaa tagcattttc gttctcgctg tcaatacttg cttcggaatc gtctcgacgg atgtcaacaa tgtatgtgga ggacatggta tatgtcaatc cacagattct tgctattgtt tggacatttg ctcaaagaac tctcttagaa ataatgcggg tgctgtttct ctcgctcaga gcaatgattt aatctccacc aacggtcgat tcaaagcatc catgcaagtg gatggtaatt ttgtgatcta tgatttgcaa tcgaatcaag ccatatggtc tactggaacg aatggacaag gttctcaacc ttattctctc gtgttggatt cgagtggagg tctacgtgtg tatggaaatg gtgctgttat ttcgaccttg gtgcagagtt gttcggcgct tccagcgacg ttgggtagtg gaagtgtttg cagtggacat ggttcttgta attcaccaaa tacttgttct tgtcaatctg gatattctgg atcaaactgt gaaacttatt attgcaatgg aattttatac aattcaacga gtgtttgtaa ttctcgtgga acttgttcag cctcagaaac ttgtgtttgc aatgcaaatt atggaggtac ctattgtcaa attcctaaat gctttggaat tttacaaaca gccactggca cagtttgcag tggacatggt acatgcaacg atgttgacac atgtgcttgc aacacgaatt ggggaggacc aagttgctcc tttcccatgt gttttggaat tttacaaaat gaaacgaata gtgtttgtca tggacatggt tcttgcaatg atgtcgatac ttgtgcatgt gtggcgggat ggggtggctc tcaatgttcc atgccaacct gcaataatat tttacagaat cagacaggaa tagtgtgtgg tggacatggt acatgcgatg gaccagacac gtgtcattgc aattctaatt ttacaggtcc tttctgtcaa tatccctttt gttatggtat tccttcaaat gcatcaaccg ccgtttgtag tggacatggt gtctgtactg gtgtcgatac ctgtgtctgt aattcgaatt gggctggatc tgattgctcc atgcctaaat gttttggagt gttgcagaac tcgaccactg tttgtagtgg acatggaact tgcaatgatg ctaatgtttg tgcttgcaat tcaaattggg acggacctga ttgctccatg cctaaatgtt ttggaatttt acaaaacatg acgagtagtg tgtgtagtgg acatggatca tgtaatgacg ttgacacgtg tttgtgtaat cctaattgga gtggttccaa ttgttcaatt cccatgtgtt ttggtatttt acaaaatcaa acgagtgaag tttgcaatgg acgtggttct tgtaatggtg tggatacctg tgtctgcaat tccaattggg gtggttccaa ttgttcaatt ccaaaatgtt ttggtatttt acaaaacatg acaggtagtg tttgcagtgg acatggttct tgtcaaaatg tggacacttg tgtgtgtgat ttgaattggg gaggaccaag ttgttcctta cctacatgct ttggaatttt acaaaacgaa acgagtagtg tttgtaatgg acatggttct tgcaatgcag tggatacttg tgtttgtaat ttgaattatg gcggtccttc ttgtgcctta cctaaatgtt ttggagtttt acaaaacatg acaagcaccg tgtgtagtgg acatggctct tgtatcgatg tgaatacttg ttcatgtttg actggatggg taggtctcaa ttgttccatg ccaacctgtt ttggaattct gcaaaattca acctcaactg tgtgtagtgg acacggctca tgtgtgaatc ttgacacttg ttcttgcatg gctggatggg gtggtccaag ttgcgcctta cctaaatgct ttggaatttt acaaaatgaa acgaatagtg tttgtcatgg acatggttct tgcaatgatg tcgatacttg tgcatgtgtg gcgggatggg gtggctctca atgttccatg ccaacctgca ataatatttt acagaatcag acaggaatag tgtgtggtgg acatggtact tgtgatgctc caaacgtgtg tcgttgcaat ccgggttgga gtggcgactt ttgtttcatg ccctcttgtt ttggaatttt acaaaatata acttccagta tttgttctgg gcatggtatt tgtaccaacg tcgatacatg cctctgtcat acgaattgga caggccttaa ttgttcgata ccaacgtgct ttggattttt acaaaactct acaaatgttt gcagtggaca tggatcatgc acgaatgtgg acacttgtgt atgtgattcg aattggggag gaccaagttg tgccttacca aaatgttttg gaattttaca aaatgtgacg agtagtgttt gcagtggaca tggatcatgt aatgcagtgg atacttgttt tgtaacggac aaggaacttg tgacacagcc gatgtttgtc gatgtaattc aaacttttct ggtgaatggt gccaatatcc caaatgttac aacactgctt ccaatgaaac tgcaacagtt tgtagtggac atggagcttg tatgtcaccc aacaactgtt cttgcttacc cacatggatt ggtgcagatt gttcagtcac cacttgcttt aatgaattgt ccaacacgtc caatgtgtgt agtggaaaag gaagttgtac atctttcgat cattgcaact gcaattcaaa ttattttgga aacaaatgtg agctcacgac ttgttatgga attacttcca acaactctct cgtttgcagt aacaatggac aatgtagctc ttttaacaat tgtacttgct cctcaggtta tgatggatcc atgtgtgaaa attatagatg ttatggaatt ccttttaact caaccaatac ttgtagtgga aaaggtatct gcgtttctcc caacaagtgc gtctggaaat tgcagtgatt ataacaattg tacttgccag tcaggttttg tgtcaatgga ttgcagtgtg aacattaatc cttatgtcat ttcattgtct tcaacatcca tgcaaattga cacttcatcc aacatgacaa gtgtcgaagt gcaaatgaat attccaaccg atgtgaatcc tctgatgacc agtttctatt ggtactgctt gaattgtcaa ggaatttcaa attcatctgt cttcatgttg ggctccaatc aacaaaaatt aaccattgcc attcgagagt tgaaggcacc tggtagctac ttgttccatg cgaatgctac caccttgaat ccttttccgt tcccaacacg aatttcaaac actgaagtgt ttaatttaac actcctagtt ccatacgtac caaatctatc tgttccttct ttaggagtat ttggtcttcc ttctgccttt gtttcgacac ggaatggagg gtcaggtcag accatcagtg ccgtcatttc gaatattgta ttgaatcaaa cgaatattga gaaattcctt ggatgtaaag caaattgtac cacctctctc acttactcat ggcaattgac aagtacaagt agcagctcat tggaagcctc ttccactgga agtatttctg cctttactgg tggcaataat ctcatgattc ttcaaccacc aattatagtg tcagccactc ttagaaattt ggtgagcaac agcaagattg gttttgcatt agcactattt aacaatcaaa tgttaaatat ttcctctcgg gtggatattc cagtgttgcc acctgtggct ccacctacaa atagttcact ccctgtcact aataatacca acgctgtgat ttcttcctta gtgcaagtgg tgccagctga aggtgtagct ctcacagata cgtttacaat taacattaca gcatggaagg caccaagaga attgcttcca ctcagatatg ccgtcggatt ttatgacaaa actaaaggaa aggacgtgag atttactgat ttttctctca atctcaccat cactgtttat ttgcccttta ttcaaaataa ggcaagaaga agaaatgtcc atgcagcatt gtcacaagtg gatttagttg tgtatgtgat ggatgctcta ggaaatgtgg attcacaatc ttcaaatgtc accgtcactg tggctccttt caacggaaca tcaacggaat tgcttcagag aattaatcaa ctttctggta cctcattgat ggtagcttcg ttcgatcaat cctatactgc attgagcagt acaagcggag gaaattcaag cgctgtgatt tatgagcttg taaagaacat tgtgattgat cctaaaaatc catcagttgc cttatccagt tttgaaagtt taacatcgaa ctcggattcg atcaatgaca atatcgtcag tgctgttacc accaagttgt cttcgtttgt tgacggagtg aaaaatactt atagtgatga aaagaaactt tacggatttg tcaagagcaa actctccgat caagatgtaa cctcaaccat ttctgtttcg tcgaatttac tgtccagtgg tgtttctagt gacaaaacaa gaaatgtcac agaaaatatt tcctcaatgg tcttattggg agaggttgca aaagtcattg accaatcaag ctctgcatat ttaccaactg tttcctatag cacttctctg atcaatatta ccatttccag ctttaaattg aattctggaa tttcgattaa tcaaacctta tcgtcaggaa gtaactcggt agagctgagc atctcacaaa ttttgacaca atacaatgca ttctcaaagg aatgtggagt gtcaatgatt gaatacgcta aaaacacaaa ctatgataac tacacttcca ataatgttat tacatcagtg gtgaacgatt tcaaatttat tcaagaacaa actccattaa tattgaataa ccttaaccag ccaatcattc tcacattcaa attggattct tcaagcttac aaggaaagaa tatttccaat tccactttta cttgtagata ttgggatgaa gagggacggc gatggagcaa caatggatgc ttcttgcatc gagtggatat tccatcaaag acgatcgaat gtgcatgtta tcacaccacc atgtttacca cattcttgga acaaaaaacc accgtcaaca tgctcgaagt gaaagatcaa gtcgccgctc tctatttttc tcagattgtc tttggatgta tctacactgc aattgccatc ttcttgttgg tgagcttgat tgtgttaaga aaggaacaac cagtgacgag tcgattgttg actccatatg tgggtcttat tgccttaatt acagagagca ttttaattta tatcattcag agaggagtat tggtcaatca attgttggga gcctctacac aaatgtggga aacaggagat actgctgcca atgtgattgc caacattgtg accatctttg tgaacacatt gaacttgtct gctattttat gctatgtctt gcaagtgttt agatttcaat tgatgaaatt cctttatcaa caaatgtcca acagtcgagg caacgagaaa aatagcgaaa gaattctcaa agcattgaag tactctactt cggctgcact gttttacacc attattgcca tatttgcagc atttaatgtt tgttattgga ctctttgggt cattcttgtg agaacaggcg caatctcctc tccaacgtat agctacattg tttccatttc gtaccttgtt tgtattttga catttagcat tgtgatttgt actgttgcca tctttgattg gattttgtcc tcaaagaaag aagtggaggt gaaaaacagt cgaagaagac caacgaacaa ctttagtcaa gtgaactcga ttgcccttgc tagcgattca ggtgaaaatc tcaaagatcc aaagaagaaa aatttaatca ctctccccac aactgtgatg aattggttta tatctttgga ccaacctctt tatttcagaa tggaaatggt tatttacatg gtttgcttta ttttccaaat tctaaatctc attattggaa gttcgagttt atcatttaga tttgatagta atgaatcgta caagaaagca ctcactctcg atggagtttc ctttatattt gaagttttct atgttgtgag ctacttgttc gtgtttggtg gctttgcatt aattgttgca ttgatttaca aattcaaacg atcgaaaaag aaacaagaac aacttcaaaa acgtaaagaa gagcaaaaga aaaatcaaga ggaactcata aataaggaaa tgcatgtcct tttggaacat gaagaaggat acaaactctt tgaagcattt tgtcaaaaag aattttcact tgagaacttg tatctctatt ccgatctgaa agcaaatcca gaaattaccc aaggtcaaca attgggagga attgtgaaat tcatgtcctt cctctacaat acctatgtca agacaggtgc cacacatgaa gtgaacattc cttccaagtg tagaaattca ttcctctatt tgtataagaa cgtgttggaa tcgagagacc aattaataag cacggacctc aaacaatctg ctgacaatta tgaaggtgat atagagctcg tcgataagga tatcaaattt gccacaaaga acaatccaat accacgtcaa gaaattgatg ctgagactgt ctctaattgt tttgaatttc tcctccgtca agtcatgctt aatattggag acaccttctc tcgtttcgtg ttcactccag aatataaatc gttccaaaag tccttggaaa tgcaacaaac gattatggat aaggtcggag tggtgtacgg catttag
Details for NafoA.19531.a.B2.GE41662
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME MHVLLEHEEG YKLFEAFCQK EFSLENLYLY SDLKANPEIT QGQQLGGIVK FMSFLYNTYV KTGATHEVNI PSKCRNSFLY LYKNVLESRD QLISTDLKQS ADNYEGDIEL VDKDIKFATK NNPIPRQEID AETVSNCFEF LLRQVMLNIG DTFSRFVFTP EYKSFQ
Validated NT Sequence
atcttactca cttattggaa cgatttatat tctggagtga acacgaaacg agagaaggtg tctccaatat taagcatgac ttgacggagg agaaattcaa aacaattaga gacagtctca gcatcaattt cttgacgtgg tattggattg ttctttgtgg caaatttgat atccttatcg acgagctcta tatcaccttc ataattgtca gcagattgtt tgaggtccgt gcttattaat tggtctctcg attccaacac gttcttatac aaatagagga atgaatttct acacttggaa ggaatgttca cttcatgtgt ggcacctgtc ttgacatagg tattgtagag gaaggacatg aatttcacaa ttcctcccaa ttgttgacct tgggtaattt ctggatttgc tttcagatcg gaatagagat acaagttctc aagtgaaaat tctttttgac aaaatgcttc aaagagtttg tatccttctt catgttccaa aaggacatgc atttccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHEM HVLLEHEEGY KLFEAFCQKE FSLENLYLYS DLKANPEITQ GQQLGGIVKF MSFLYNTYVK TGATHEVNIP SKCRNSFLYL YKNVLESRDQ LISTDLKQSA DNYEGDIELV DKDIKFATKN NPIPRQEIDA ETVSNCFEFL LRQVMLNIGD TFSRFVFTPE YKSFQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaatgc atgtcctttt ggaacatgaa gaaggataca aactctttga agcattttgt caaaaagaat tttcacttga gaacttgtat ctctattccg atctgaaagc aaatccagaa attacccaag gtcaacaatt gggaggaatt gtgaaattca tgtccttcct ctacaatacc tatgtcaaga caggtgccac acatgaagtg aacattcctt ccaagtgtag aaattcattc ctctatttgt ataagaacgt gttggaatcg agagaccaat taataagcac ggacctcaaa caatctgctg acaattatga aggtgatata gagctcgtcg ataaggatat caaatttgcc acaaagaaca atccaatacc acgtcaagaa attgatgctg agactgtctc taattgtttt gaatttctcc tccgtcaagt catgcttaat attggagaca ccttctctcg tttcgtgttc actccagaat ataaatcgtt ccaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat