NafoA.19247.a

geranylgeranyl transferase type-2 subunit beta-like

CENTER ID: NafoA.19247.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.19247.a.B1.GE40633 Full length(NafoA.19247.a) 1 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.19247.a.B1.PS38170 Full length(NafoA.19247.a) 1 349

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0125870

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSSVQAFAK EAHTSYIQHL EEKETNTFEY CVTEHLRMSG MYWGLTAMDL LNEIDKMNRD KILQFVRDSY HEEEGGFSGA PNHDPHILYT LSAVQLLVLL TDNIEEFLSL EQIEKIGSYI GSLQKEDGSF AGDQWGEVDT RFSYCALNCL ALLGLLETCS NYRGDKINNR KFINVEKAVQ YVLSCQNFDG GFGVCPGAES HAGQIFTCIG ALSIGKALDR IDQDTLCWWL CERQCDNGGL NGRPEKLADV CYSWWVLSAL GMMDRIHWID EAKLHDYICN CQDTEKGGIS DKPNNAVDVF HTFFGIAGLS LLGYGEKYAL KTIDPTFALP VGALKKIGIQ TPYTLRYDQ
NT Sequence
ATGTCTTCAT CGGTCCAAGC CTTCGCAAAA GAGGCTCACA CATCCTACAT TCAACATTTG GAGGAGAAGG AAACCAATAC CTTCGAGTAT TGTGTTACGG AACATTTGAG AATGTCCGGA ATGTATTGGG GATTAACAGC AATGGATCTC CTAAATGAAA TAGACAAGAT GAATCGAGAC AAGATTTTGC AGTTTGTGAG AGATTCCTAT CATGAGGAAG AGGGTGGCTT TTCGGGTGCT CCGAATCATG ATCCTCATAT TCTCTATACT CTCTCTGCAG TACAATTATT GGTTTTATTG ACCGATAATA TTGAAGAGTT TTTAAGCCTT GAACAAATTG AAAAGATTGG TTCCTATATT GGATCGTTGC AAAAAGAAGA TGGTAGCTTT GCAGGTGATC AATGGGGAGA AGTGGATACA CGATTCAGTT ATTGTGCTTT GAATTGTTTG GCTTTATTAG GACTATTGGA AACATGTAGT AATTATAGAG GAGACAAGAT CAATAATCGA AAATTTATTA ACGTCGAAAA GGCAGTGCAA TATGTATTGT CATGTCAAAA TTTTGATGGA GGTTTTGGAG TTTGCCCCGG AGCTGAATCC CATGCTGGTC AAATTTTCAC ATGTATCGGA GCATTGTCCA TTGGAAAAGC ATTGGATAGA ATCGACCAAG ACACACTTTG TTGGTGGCTA TGTGAGAGAC AGTGTGACAA TGGTGGATTG AATGGACGCC CAGAAAAATT GGCTGATGTC TGTTATTCAT GGTGGGTATT GAGTGCTTTG GGAATGATGG ATCGTATTCA TTGGATTGAT GAAGCGAAAT TGCACGATTA CATTTGCAAT TGCCAGGACA CTGAAAAGGG TGGAATCAGT GACAAGCCCA ATAATGCGGT AGATGTATTC CATACATTTT TCGGAATTGC TGGATTGAGT TTGTTAGGAT ATGGCGAAAA GTATGCTTTG AAAACAATTG ATCCAACGTT TGCCTTACCT GTTGGAGCAC TCAAAAAGAT TGGTATTCAA ACACCTTATA CTTTGCGATA TGATCAA
Details for NafoA.19247.a.B1.GE40633
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS SSVQAFAKEA HTSYIQHLEE KETNTFEYCV TEHLRMSGMY WGLTAMDLLN EIDKMNRDKI LQFVRDSYHE EEGGFSGAPN HDPHILYTLS AVQLLVLLTD NIEEFLSLEQ IEKIGSYIGS LQKEDGSFAG DQWGEVDTRF SYCALNCLAL LGLLETCSNY RGDKINNRKF INVEKAVQYV LSCQNFDGGF GVCPGAESHA GQIFTCIGAL SIGKALDRID QDTLCWWLCE RQCDNGGLNG RPEKLADVCY SWWVLSALGM MDRIHWIDEA KLHDYICNCQ DTEKGGISDK PNNAVDVFHT FFGIAGLSLL GYGEKYALKT IDPTFALPVG ALKKIGIQ
Validated NT Sequence
gtttgaatac caatcttttt gagtgctcca acaggtaagg caaacgttgg atcaattgtt ttcaaagcat acttttcgcc atatcctaac aaactcaatc cagcaattcc gaaaaatgta tggaatacat ctaccgcatt attgggcttg tcactgattc cacccttttc agtgtcctgg caattgcaaa tgtaatcgtg caatttcgct tcatcaatcc aatgaatacg atccatcatt cccaaagcac tcaataccca ccatgaataa cagacatcag ccaatttttc tgggcgtcca ttcaatccac cattgtcaca ctgtctctca catagccacc aacaaagtgt gtcttggtcg attctatcca atgcttttcc aatggacaat gctccgatac atgtgaaaat ttgaccagca tgggattcag ctccggggca aactccaaaa cctccatcaa aattttgaca tgacaataca tattgcactg ccttttcgac gttaataaat tttcgattat tgatcttgtc tcctctataa ttactacatg tttccaatag tcctaataaa gccaaacaat tcaaagcaca ataactgaat cgtgtatcca cttctcccca ttgatcacct gcaaagctac catcttcttt ttgcaacgat ccaatatagg aaccaatctt ttcaatttgt tcaaggctta aaaactcttc aatattatcg gtcaataaaa ccaataattg tactgcagag agagtataga gaatatgagg atcatgattc ggagcacccg aaaagccacc ctcttcctca tgataggaat ctctcacaaa ctgcaaaatc ttgtctcgat tcatcttgtc tatttcattt aggagatcca ttgctgttaa tccccaatac attccggaca ttctcaaatg ttccgtaaca caatactcga aggtattggt ttccttctcc tccaaatgtt gaatgtagga tgtgtgagcc tcttttgcga aggcttggac cgatgaagac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMS SSVQAFAKEA HTSYIQHLEE KETNTFEYCV TEHLRMSGMY WGLTAMDLLN EIDKMNRDKI LQFVRDSYHE EEGGFSGAPN HDPHILYTLS AVQLLVLLTD NIEEFLSLEQ IEKIGSYIGS LQKEDGSFAG DQWGEVDTRF SYCALNCLAL LGLLETCSNY RGDKINNRKF INVEKAVQYV LSCQNFDGGF GVCPGAESHA GQIFTCIGAL SIGKALDRID QDTLCWWLCE RQCDNGGLNG RPEKLADVCY SWWVLSALGM MDRIHWIDEA KLHDYICNCQ DTEKGGISDK PNNAVDVFHT FFGIAGLSLL GYGEKYALKT IDPTFALPVG ALKKIGIQTP YTLRYDQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt catcggtcca agccttcgca aaagaggctc acacatccta cattcaacat ttggaggaga aggaaaccaa taccttcgag tattgtgtta cggaacattt gagaatgtcc ggaatgtatt ggggattaac agcaatggat ctcctaaatg aaatagacaa gatgaatcga gacaagattt tgcagtttgt gagagattcc tatcatgagg aagagggtgg cttttcgggt gctccgaatc atgatcctca tattctctat actctctctg cagtacaatt attggtttta ttgaccgata atattgaaga gtttttaagc cttgaacaaa ttgaaaagat tggttcctat attggatcgt tgcaaaaaga agatggtagc tttgcaggtg atcaatgggg agaagtggat acacgattca gttattgtgc tttgaattgt ttggctttat taggactatt ggaaacatgt agtaattata gaggagacaa gatcaataat cgaaaattta ttaacgtcga aaaggcagtg caatatgtat tgtcatgtca aaattttgat ggaggttttg gagtttgccc cggagctgaa tcccatgctg gtcaaatttt cacatgtatc ggagcattgt ccattggaaa agcattggat agaatcgacc aagacacact ttgttggtgg ctatgtgaga gacagtgtga caatggtgga ttgaatggac gcccagaaaa attggctgat gtctgttatt catggtgggt attgagtgct ttgggaatga tggatcgtat tcattggatt gatgaagcga aattgcacga ttacatttgc aattgccagg acactgaaaa gggtggaatc agtgacaagc ccaataatgc ggtagatgta ttccatacat ttttcggaat tgctggattg agtttgttag gatatggcga aaagtatgct ttgaaaacaa ttgatccaac gtttgcctta cctgttggag cactcaaaaa gattggtatt caaacacctt atactttgcg atatgatcaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NafoA.19247.a.B1.PS38170
PURIFICATION DATe: 1/17/2017
CONCENTRATION: 42mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS SSVQAFAKEA HTSYIQHLEE KETNTFEYCV TEHLRMSGMY WGLTAMDLLN EIDKMNRDKI LQFVRDSYHE EEGGFSGAPN HDPHILYTLS AVQLLVLLTD NIEEFLSLEQ IEKIGSYIGS LQKEDGSFAG DQWGEVDTRF SYCALNCLAL LGLLETCSNY RGDKINNRKF INVEKAVQYV LSCQNFDGGF GVCPGAESHA GQIFTCIGAL SIGKALDRID QDTLCWWLCE RQCDNGGLNG RPEKLADVCY SWWVLSALGM MDRIHWIDEA KLHDYICNCQ DTEKGGISDK PNNAVDVFHT FFGIAGLSLL GYGEKYALKT IDPTFALPVG ALKKIGIQ
Validated NT Sequence
gtttgaatac caatcttttt gagtgctcca acaggtaagg caaacgttgg atcaattgtt ttcaaagcat acttttcgcc atatcctaac aaactcaatc cagcaattcc gaaaaatgta tggaatacat ctaccgcatt attgggcttg tcactgattc cacccttttc agtgtcctgg caattgcaaa tgtaatcgtg caatttcgct tcatcaatcc aatgaatacg atccatcatt cccaaagcac tcaataccca ccatgaataa cagacatcag ccaatttttc tgggcgtcca ttcaatccac cattgtcaca ctgtctctca catagccacc aacaaagtgt gtcttggtcg attctatcca atgcttttcc aatggacaat gctccgatac atgtgaaaat ttgaccagca tgggattcag ctccggggca aactccaaaa cctccatcaa aattttgaca tgacaataca tattgcactg ccttttcgac gttaataaat tttcgattat tgatcttgtc tcctctataa ttactacatg tttccaatag tcctaataaa gccaaacaat tcaaagcaca ataactgaat cgtgtatcca cttctcccca ttgatcacct gcaaagctac catcttcttt ttgcaacgat ccaatatagg aaccaatctt ttcaatttgt tcaaggctta aaaactcttc aatattatcg gtcaataaaa ccaataattg tactgcagag agagtataga gaatatgagg atcatgattc ggagcacccg aaaagccacc ctcttcctca tgataggaat ctctcacaaa ctgcaaaatc ttgtctcgat tcatcttgtc tatttcattt aggagatcca ttgctgttaa tccccaatac attccggaca ttctcaaatg ttccgtaaca caatactcga aggtattggt ttccttctcc tccaaatgtt gaatgtagga tgtgtgagcc tcttttgcga aggcttggac cgatgaagac atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMS SSVQAFAKEA HTSYIQHLEE KETNTFEYCV TEHLRMSGMY WGLTAMDLLN EIDKMNRDKI LQFVRDSYHE EEGGFSGAPN HDPHILYTLS AVQLLVLLTD NIEEFLSLEQ IEKIGSYIGS LQKEDGSFAG DQWGEVDTRF SYCALNCLAL LGLLETCSNY RGDKINNRKF INVEKAVQYV LSCQNFDGGF GVCPGAESHA GQIFTCIGAL SIGKALDRID QDTLCWWLCE RQCDNGGLNG RPEKLADVCY SWWVLSALGM MDRIHWIDEA KLHDYICNCQ DTEKGGISDK PNNAVDVFHT FFGIAGLSLL GYGEKYALKT IDPTFALPVG ALKKIGIQTP YTLRYDQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTT CATCGGTCCA AGCCTTCGCA AAAGAGGCTC ACACATCCTA CATTCAACAT TTGGAGGAGA AGGAAACCAA TACCTTCGAG TATTGTGTTA CGGAACATTT GAGAATGTCC GGAATGTATT GGGGATTAAC AGCAATGGAT CTCCTAAATG AAATAGACAA GATGAATCGA GACAAGATTT TGCAGTTTGT GAGAGATTCC TATCATGAGG AAGAGGGTGG CTTTTCGGGT GCTCCGAATC ATGATCCTCA TATTCTCTAT ACTCTCTCTG CAGTACAATT ATTGGTTTTA TTGACCGATA ATATTGAAGA GTTTTTAAGC CTTGAACAAA TTGAAAAGAT TGGTTCCTAT ATTGGATCGT TGCAAAAAGA AGATGGTAGC TTTGCAGGTG ATCAATGGGG AGAAGTGGAT ACACGATTCA GTTATTGTGC TTTGAATTGT TTGGCTTTAT TAGGACTATT GGAAACATGT AGTAATTATA GAGGAGACAA GATCAATAAT CGAAAATTTA TTAACGTCGA AAAGGCAGTG CAATATGTAT TGTCATGTCA AAATTTTGAT GGAGGTTTTG GAGTTTGCCC CGGAGCTGAA TCCCATGCTG GTCAAATTTT CACATGTATC GGAGCATTGT CCATTGGAAA AGCATTGGAT AGAATCGACC AAGACACACT TTGTTGGTGG CTATGTGAGA GACAGTGTGA CAATGGTGGA TTGAATGGAC GCCCAGAAAA ATTGGCTGAT GTCTGTTATT CATGGTGGGT ATTGAGTGCT TTGGGAATGA TGGATCGTAT TCATTGGATT GATGAAGCGA AATTGCACGA TTACATTTGC AATTGCCAGG ACACTGAAAA GGGTGGAATC AGTGACAAGC CCAATAATGC GGTAGATGTA TTCCATACAT TTTTCGGAAT TGCTGGATTG AGTTTGTTAG GATATGGCGA AAAGTATGCT TTGAAAACAA TTGATCCAAC GTTTGCCTTA CCTGTTGGAG CACTCAAAAA GATTGGTATT CAAACACCTT ATACTTTGCG ATATGATCAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT