NafoA.18809.a

3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR)

CENTER ID:	NafoA.18809.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.18809.a.B2.GE41829	C-term sim to SmHMGR cat domain		55	483
NafoA.18809.a.B3.GE41830	XtalPred shorter C-term domain		98	483

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.18809.a.B2.PW38407	C-term sim to SmHMGR cat domain		55	483

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0036310

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTNRLFRVL ILVFIHGSIE KIQVVTEIGN NVCAVRIEET SEEAIQRELE QEKEEHEEAK RKEQERYRQL SDGAIIDLLE NGKLALYNLE DKLGDKKRAV KIRRMFLAAK INPQDETILD TLPYANDLNY DHVFGACCES VVGYVPLPVG IVGPIIIDGK PYHIPMATVE GCLVASTHRG CKALSSSLEG GVNSVILSDG MTRGPVVQFA NAKRCYELKQ YIENPENFQT ICRVFNSDSR FARLLEIKVG IAGRKAFLRF KCRTGDAMGM NMISKATQKA LKFLKHELFP DMDILSLSGN YCTDKKPSAM NWIEGRGKSV VVDATIPADV VKSVLKTTVD EIVSLNVSKN LIGSAMAGSI GGFNAHAANI VTAIFLATGQ DAAQNVESSN CMTIMEKTPE GHLYVSVTMP SIEVGTVGGG THLDAQKTCL KIMGVAGANM ENPGQNAQTL AKCVASTVMA GELSLMAALA ADHLVSAHIA HNRKK

NT Sequence

atgtcaacaa atcgtctgtt ccgtgttctt attcttgtgt tcatacatgg cagcattgaa aaaattcaag tagtgactga gattggtaat aatgtgtgtg ctgttagaat cgaagaaacc tcagaagaag cgattcaacg agaattggaa caagagaagg aggaacacga agaagcaaag agaaaggaac aagaaagata tagacaatta tctgatggag ctatcattga tttattagag aatggaaagc tcgctctcta caatctcgaa gataagcttg gagataagaa gcgtgctgtt aaaatccgta gaatgttcct tgcagcaaaa atcaatccac aagatgaaac aatactagat acacttcctt atgctaatga tttaaattat gatcatgtgt ttggagcttg ttgtgaaagt gtggttggtt atgttccttt acctgttggt atcgttggtc caataataat tgatggtaag ccatatcata ttccaatggc tactgttgaa ggctgtcttg ttgccagtac tcatagaggt tgcaaggctc tttcgagctc attagaagga ggagtgaaca gtgtcatcct ctcagacgga atgactagag gtccagtcgt tcaattcgct aatgcaaaga gatgttatga actcaaacaa tacattgaga atccagaaaa tttccaaacc atttgccgag ttttcaacag tgattctcgt tttgctagat tattagaaat taaggtcggt attgctggaa gaaaggcttt cttgagattt aaatgtagga cgggtgatgc catgggtatg aatatgatct ctaaagccac acaaaaagca ttgaaatttt tgaaacacga actcttccct gatatggata ttttaagctt gagtggtaac tactgcacag acaaaaagcc atcagcaatg aactggattg aaggaagagg aaaatctgtg gttgttgatg ctactattcc agcagatgtt gtgaaatcag ttctcaagac tactgtagac gaaattgtga gtctcaatgt gagcaagaac ttgattggaa gcgctatggc tggctctatt ggtggtttca acgctcatgc cgctaacatt gtgaccgcca tctttttagc aactggtcaa gacgctgctc aaaacgtcga aagttcgaat tgtatgacaa taatggaaaa gactccagaa ggacatttat atgtaagcgt gactatgcca tccatcgaag ttggaactgt tggtggtggt actcacttgg atgctcaaaa gacctgtctc aagattatgg gagttgcagg cgcaaatatg gaaaacccag gtcaaaatgc tcaaacgctt gccaagtgcg tggccagtac cgtcatggca ggtgaacttt ctttaatggc tgctcttgct gctgatcatt tagtttctgc ccacattgct cacaacagaa agaagtaa

Details for NafoA.18809.a.B2.GE41829

HARVESTED ON:	8/7/2017
SEQUENCED ON:	9/20/2017
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME HEEAKRKEQE RYRQLSDGAI IDLLENGKLA LYNLEDKLGD KKRAVKIRRM FLAAKINPQD ETILDTLPYA NDLNYDHVFG ACCESVVGYV PLPVGIVGPI IIDGKPYHIP MATVEGCLVA STHRGCKALS SSLEGGVNSV ILSDGMTRGP VVQFANAKRC YELKQYIENP ENFQTICQVF NSDSRFARLL EIKVGIAGRK AFLRFKCRTG DAMGMNMISK ATQKALKFLK HELFPDMDIL SLSGNYCTDK KPSAMNWIEG RGKSVVVDAT IPADVVKSVL KTTVDEIVSL NVSKNLIGSA MAGSIGGFNA HAANIVTAIF LATGQDAAQN VESSNCMTIM EKTPEGHLYV SVTMPSIEVG TVGGGTHLDA QKTCLKIMGV AGANMENPGQ NAQTLAKCVA STVMAGELSL MAALAADHLV SAHIAHNR

Validated NT Sequence

atggctcacc accaccacca ccatatggaa cacgaagaag caaagagaaa ggaacaagaa agatatagac aattatctga tggagctatc attgatttat tagagaatgg aaagctcgct ctctacaatc tcgaagataa gcttggagat aagaagcgtg ctgttaaaat ccgtagaatg ttccttgcag caaaaatcaa tccacaagat gaaacaatac tagatacact tccttatgct aatgatttaa attatgatca tgtgtttgga gcttgttgtg aaagtgtggt tggttatgtt cctttacctg ttggtatcgt tggtccaata ataattgatg gtaagccata tcatattcca atggctactg ttgaaggctg tcttgttgcc agtactcata gaggttgcaa ggctctttcg agctcattag aaggaggagt gaacagtgtc atcctctcag acggaatgac tagaggtcca gtcgttcaat tcgctaatgc aaagagatgt tatgaactca aacaatacat tgagaatcca gaaaatttcc aaaccatttg ccaagttttc aacagtgatt ctcgttttgc tagattatta gaaattaagg tcggtattgc tggaagaaag gctttcttga gatttaaatg taggacgggt gatgccatgg gtatgaatat gatctctaaa gccacacaaa aagcattgaa atttttgaaa cacgaactct tccctgatat ggatatttta agcttgagtg gtaactactg cacagacaaa aagccatcag caatgaactg gattgaagga agaggaaaat ctgtggttgt tgatgctact attccagcag atgttgtgaa atcagttctc aagactactg tagacgaaat tgtgagtctc aatgtgagca agaacttgat tggaagcgct atggctggct ctattggtgg tttcaacgct catgccgcta acattgtgac cgccatcttt ttagcaactg gtcaagacgc tgctcaaaac gtcgaaagtt cgaattgtat gacaataatg gaaaagactc cagaaggaca tttatatgta agcgtgacta tgccatccat cgaagttgga actgttggtg gtggtactca cttggatgct caaaagacct gtctcaagat tatgggagtt gcaggcgcaa atatggaaaa cccaggtcaa aatgctcaaa cgcttgccaa gtgcgtggcc agtaccgtca tggcaggtga actttcttta atggctgctc ttgctgctga tcatttagtt tctgcccaca ttgctcacaa cagataa

Expected Protein Sequence

MAHHHHHHEH EEAKRKEQER YRQLSDGAII DLLENGKLAL YNLEDKLGDK KRAVKIRRMF LAAKINPQDE TILDTLPYAN DLNYDHVFGA CCESVVGYVP LPVGIVGPII IDGKPYHIPM ATVEGCLVAS THRGCKALSS SLEGGVNSVI LSDGMTRGPV VQFANAKRCY ELKQYIENPE NFQTICRVFN SDSRFARLLE IKVGIAGRKA FLRFKCRTGD AMGMNMISKA TQKALKFLKH ELFPDMDILS LSGNYCTDKK PSAMNWIEGR GKSVVVDATI PADVVKSVLK TTVDEIVSLN VSKNLIGSAM AGSIGGFNAH AANIVTAIFL ATGQDAAQNV ESSNCMTIME KTPEGHLYVS VTMPSIEVGT VGGGTHLDAQ KTCLKIMGVA GANMENPGQN AQTLAKCVAS TVMAGELSLM AALAADHLVS AHIAHNR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaacacg aagaagcaaa gagaaaggaa caagaaagat atagacaatt atctgatgga gctatcattg atttattaga gaatggaaag ctcgctctct acaatctcga agataagctt ggagataaga agcgtgctgt taaaatccgt agaatgttcc ttgcagcaaa aatcaatcca caagatgaaa caatactaga tacacttcct tatgctaatg atttaaatta tgatcatgtg tttggagctt gttgtgaaag tgtggttggt tatgttcctt tacctgttgg tatcgttggt ccaataataa ttgatggtaa gccatatcat attccaatgg ctactgttga aggctgtctt gttgccagta ctcatagagg ttgcaaggct ctttcgagct cattagaagg aggagtgaac agtgtcatcc tctcagacgg aatgactaga ggtccagtcg ttcaattcgc taatgcaaag agatgttatg aactcaaaca atacattgag aatccagaaa atttccaaac catttgccga gttttcaaca gtgattctcg ttttgctaga ttattagaaa ttaaggtcgg tattgctgga agaaaggctt tcttgagatt taaatgtagg acgggtgatg ccatgggtat gaatatgatc tctaaagcca cacaaaaagc attgaaattt ttgaaacacg aactcttccc tgatatggat attttaagct tgagtggtaa ctactgcaca gacaaaaagc catcagcaat gaactggatt gaaggaagag gaaaatctgt ggttgttgat gctactattc cagcagatgt tgtgaaatca gttctcaaga ctactgtaga cgaaattgtg agtctcaatg tgagcaagaa cttgattgga agcgctatgg ctggctctat tggtggtttc aacgctcatg ccgctaacat tgtgaccgcc atctttttag caactggtca agacgctgct caaaacgtcg aaagttcgaa ttgtatgaca ataatggaaa agactccaga aggacattta tatgtaagcg tgactatgcc atccatcgaa gttggaactg ttggtggtgg tactcacttg gatgctcaaa agacctgtct caagattatg ggagttgcag gcgcaaatat ggaaaaccca ggtcaaaatg ctcaaacgct tgccaagtgc gtggccagta ccgtcatggc aggtgaactt tctttaatgg ctgctcttgc tgctgatcat ttagtttctg cccacattgc tcacaacaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for NafoA.18809.a.B3.GE41830

HARVESTED ON:	8/7/2017
SEQUENCED ON:	9/20/2017
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR AVKIRRMFLA AKINPQDETI LDTLPYANDL NYDHVFGACC ESVVGYVPLP VGIVGPIIID GKPYHIPMAT VEGCLVASTH RGCKALSSSL EGGVNSVILS DGMTRGPVVQ FANAKRCYEL KQYIENPENF QTICRVFNSD SRFARLLEIK VGIAGRKAFL RFKCRTGDAM GMNMISKATQ KALKFLKHEL FPDMDILSLS GNYCTDKKPS AMNWIEGRGK SVVVDATIPA DVVKSVLKTT VDEIVSLNVS KNLIGSAMAG SIGGFNAHAA NIVTAIFLAT GQDAAQNVES SNCMTIMEKT PEGHLYVSVT MPSIEVGTVG GGTHLDAQKT CLKIMGVAGA NMENPGQNAQ TLAKCVASTV MAGELSLMAA LAADHLVSAH IAHNR

Validated NT Sequence

atggctcacc accaccacca ccatatgcgt gctgttaaaa tccgtagaat gttccttgca gcaaaaatca atccacaaga tgaaacaata ctagatacac ttccttatgc taatgattta aattatgatc atgtgtttgg agcttgttgt gaaagtgtgg ttggttatgt tcctttacct gttggtatcg ttggtccaat aataattgat ggtaagccat atcatattcc aatggctact gttgaaggct gtcttgttgc cagtactcat agaggttgca aggctctttc gagctcatta gaaggaggag tgaacagtgt catcctctca gacggaatga ctagaggtcc agtcgttcaa ttcgctaatg caaagagatg ttatgaactc aaacaataca ttgagaatcc agaaaatttc caaaccattt gccgagtttt caacagtgat tctcgttttg ctagattatt agaaattaag gtcggtattg ctggaagaaa ggctttcttg agatttaaat gtaggacggg tgatgccatg ggtatgaata tgatctctaa agccacacaa aaagcattga aatttttgaa acacgaactc ttccctgata tggatatttt aagcttgagt ggtaactact gcacagacaa aaagccatca gcaatgaact ggattgaagg aagaggaaaa tctgtggttg ttgatgctac tattccagca gatgttgtga aatcagttct caagactact gtagacgaaa ttgtgagtct caatgtgagc aagaacttga ttggaagcgc tatggctggc tctattggtg gtttcaacgc tcatgccgct aacattgtga ccgccatctt tttagcaact ggtcaagacg ctgctcaaaa cgtcgaaagt tcgaattgta tgacaataat ggaaaagact ccagaaggac atttatatgt aagcgtgact atgccatcca tcgaagttgg aactgttggt ggtggtactc acttggatgc tcaaaagacc tgtctcaaga ttatgggagt tgcaggcgca aatatggaaa acccaggtca aaatgctcaa acgcttgcca agtgcgtggc cagtaccgtc atggcaggtg aactttcttt aatggctgct cttgctgctg atcatttagt ttctgcccac attgctcaca acagataa

Expected Protein Sequence

MAHHHHHHRA VKIRRMFLAA KINPQDETIL DTLPYANDLN YDHVFGACCE SVVGYVPLPV GIVGPIIIDG KPYHIPMATV EGCLVASTHR GCKALSSSLE GGVNSVILSD GMTRGPVVQF ANAKRCYELK QYIENPENFQ TICRVFNSDS RFARLLEIKV GIAGRKAFLR FKCRTGDAMG MNMISKATQK ALKFLKHELF PDMDILSLSG NYCTDKKPSA MNWIEGRGKS VVVDATIPAD VVKSVLKTTV DEIVSLNVSK NLIGSAMAGS IGGFNAHAAN IVTAIFLATG QDAAQNVESS NCMTIMEKTP EGHLYVSVTM PSIEVGTVGG GTHLDAQKTC LKIMGVAGAN MENPGQNAQT LAKCVASTVM AGELSLMAAL AADHLVSAHI AHNR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgtgctg ttaaaatccg tagaatgttc cttgcagcaa aaatcaatcc acaagatgaa acaatactag atacacttcc ttatgctaat gatttaaatt atgatcatgt gtttggagct tgttgtgaaa gtgtggttgg ttatgttcct ttacctgttg gtatcgttgg tccaataata attgatggta agccatatca tattccaatg gctactgttg aaggctgtct tgttgccagt actcatagag gttgcaaggc tctttcgagc tcattagaag gaggagtgaa cagtgtcatc ctctcagacg gaatgactag aggtccagtc gttcaattcg ctaatgcaaa gagatgttat gaactcaaac aatacattga gaatccagaa aatttccaaa ccatttgccg agttttcaac agtgattctc gttttgctag attattagaa attaaggtcg gtattgctgg aagaaaggct ttcttgagat ttaaatgtag gacgggtgat gccatgggta tgaatatgat ctctaaagcc acacaaaaag cattgaaatt tttgaaacac gaactcttcc ctgatatgga tattttaagc ttgagtggta actactgcac agacaaaaag ccatcagcaa tgaactggat tgaaggaaga ggaaaatctg tggttgttga tgctactatt ccagcagatg ttgtgaaatc agttctcaag actactgtag acgaaattgt gagtctcaat gtgagcaaga acttgattgg aagcgctatg gctggctcta ttggtggttt caacgctcat gccgctaaca ttgtgaccgc catcttttta gcaactggtc aagacgctgc tcaaaacgtc gaaagttcga attgtatgac aataatggaa aagactccag aaggacattt atatgtaagc gtgactatgc catccatcga agttggaact gttggtggtg gtactcactt ggatgctcaa aagacctgtc tcaagattat gggagttgca ggcgcaaata tggaaaaccc aggtcaaaat gctcaaacgc ttgccaagtg cgtggccagt accgtcatgg caggtgaact ttctttaatg gctgctcttg ctgctgatca tttagtttct gcccacattg ctcacaacag atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for NafoA.18809.a.B2.PW38407

PURIFICATION DATe:	12/12/2017
CONCENTRATION:	25.03mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAACACG AAGAAGCAAA GAGAAAGGAA CAAGAAAGAT ATAGACAATT ATCTGATGGA GCTATCATTG ATTTATTAGA GAATGGAAAG CTCGCTCTCT ACAATCTCGA AGATAAGCTT GGAGATAAGA AGCGTGCTGT TAAAATCCGT AGAATGTTCC TTGCAGCAAA AATCAATCCA CAAGATGAAA CAATACTAGA TACACTTCCT TATGCTAATG ATTTAAATTA TGATCATGTG TTTGGAGCTT GTTGTGAAAG TGTGGTTGGT TATGTTCCTT TACCTGTTGG TATCGTTGGT CCAATAATAA TTGATGGTAA GCCATATCAT ATTCCAATGG CTACTGTTGA AGGCTGTCTT GTTGCCAGTA CTCATAGAGG TTGCAAGGCT CTTTCGAGCT CATTAGAAGG AGGAGTGAAC AGTGTCATCC TCTCAGACGG AATGACTAGA GGTCCAGTCG TTCAATTCGC TAATGCAAAG AGATGTTATG AACTCAAACA ATACATTGAG AATCCAGAAA ATTTCCAAAC CATTTGCCGA GTTTTCAACA GTGATTCTCG TTTTGCTAGA TTATTAGAAA TTAAGGTCGG TATTGCTGGA AGAAAGGCTT TCTTGAGATT TAAATGTAGG ACGGGTGATG CCATGGGTAT GAATATGATC TCTAAAGCCA CACAAAAAGC ATTGAAATTT TTGAAACACG AACTCTTCCC TGATATGGAT ATTTTAAGCT TGAGTGGTAA CTACTGCACA GACAAAAAGC CATCAGCAAT GAACTGGATT GAAGGAAGAG GAAAATCTGT GGTTGTTGAT GCTACTATTC CAGCAGATGT TGTGAAATCA GTTCTCAAGA CTACTGTAGA CGAAATTGTG AGTCTCAATG TGAGCAAGAA CTTGATTGGA AGCGCTATGG CTGGCTCTAT TGGTGGTTTC AACGCTCATG CCGCTAACAT TGTGACCGCC ATCTTTTTAG CAACTGGTCA AGACGCTGCT CAAAACGTCG AAAGTTCGAA TTGTATGACA ATAATGGAAA AGACTCCAGA AGGACATTTA TATGTAAGCG TGACTATGCC ATCCATCGAA GTTGGAACTG TTGGTGGTGG TACTCACTTG GATGCTCAAA AGACCTGTCT CAAGATTATG GGAGTTGCAG GCGCAAATAT GGAAAACCCA GGTCAAAATG CTCAAACGCT TGCCAAGTGC GTGGCCAGTA CCGTCATGGC AGGTGAACTT TCTTTAATGG CTGCTCTTGC TGCTGATCAT TTAGTTTCTG CCCACATTGC TCACAACAGA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT