NafoA.18681.a
prolyl-trna synthetase
| CENTER ID: | NafoA.18681.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.18681.a.B2.GE40322 | ordered domain | 31 | 535 | ||
| NafoA.18681.a.B3.GE42995 | rescue 2 | 28 | 477 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.18681.a.B2.PW38555 | ordered domain | 31 | 535 | ||
| NafoA.18681.a.B2.PW38711 | ordered domain | 31 | 535 |
Structures
6NAB
DEPOSITED: 12/5/2018
DETERMINATION: XRay
CLONE: NafoA.18681.a.B2.GE40322
PROTEIN: NafoA.18681.a.B2.PW37988
6UYH
DEPOSITED: 11/13/2019
DETERMINATION: XRay
CLONE: NafoA.18681.a.B2.GE40322
PROTEIN: NafoA.18681.a.B2.PW37988
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0061610 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDQPASSNN NKEKKSKPAK QQQQGSSSGL KQNEGIDVKK QENFSEWYSQ VITKSEFLDY YDVSGCYIFR PNCWFVWESV QKFFDAEIKK LGVQNVMFPL FVTKRALETE KDHVEGFSPE VAWVTKSGNS DLQEPIALRP TSETIMYPSY AKWIQSHRDL PLKLNQWTNV VRWEFKHAVP FIRSREFYWQ EGHSAFKSKE EADEEVFTIL ELYKRVYEEL LAVPVIKGTK TENEKFAGAD YTTTVETFIA TNGRAVQGGT SHHLGQNFSK MFKIQFEAEN KETQFAYQNS WGLSTRTLGV MIMVHGDDKG MVLPPRVAFC QVVVIPLIFK DKDNATLVEK TKEIYNELEK AGIRVKLDDR LERTPGWKYN YWELRGVPLR IEVGPKDLEK QQIMLCRRDT GEKWTMPLSE FSGDSIKAVL DKIHDSMLNK ARKEMNERIV VTRTWPEFIK ALNSGNMCLI PWHESKAAEE YIKEKSKLES VQSQSDANTG LTGAAKSLCV PLDQSSFPSL EGLENFYPEE AHKKPNCWAL FGRSY
NT Sequence
ATGTCCGATC AACCAGCATC ATCCAACAAT AACAAGGAGA AGAAATCCAA ACCAGCAAAG CAACAACAAC AAGGTTCTTC TTCTGGTCTC AAACAAAACG AAGGTATTGA TGTCAAGAAG CAAGAAAACT TTAGCGAATG GTACAGCCAA GTCATCACCA AGAGTGAATT CTTGGATTAC TATGATGTGA GTGGATGTTA TATTTTCAGA CCAAATTGTT GGTTTGTTTG GGAGTCTGTT CAAAAGTTCT TTGATGCAGA AATTAAAAAG TTGGGTGTTC AAAATGTCAT GTTTCCATTG TTTGTTACCA AGAGAGCTTT GGAAACTGAG AAGGACCACG TTGAAGGCTT CTCTCCAGAA GTTGCTTGGG TCACCAAGTC CGGTAATTCG GATCTTCAAG AACCAATTGC TTTGAGACCA ACAAGTGAAA CCATCATGTA TCCATCGTAT GCCAAGTGGA TTCAATCTCA CAGAGATTTG CCATTAAAAT TGAACCAATG GACAAACGTT GTTAGATGGG AATTTAAACA TGCTGTTCCT TTCATTAGAT CCAGAGAATT CTACTGGCAA GAAGGTCACA GTGCTTTCAA ATCAAAGGAA GAAGCTGATG AAGAAGTTTT TACTATTTTG GAGTTATATA AAAGAGTTTA CGAAGAATTG TTGGCTGTTC CAGTCATCAA GGGTACCAAG ACTGAAAATG AAAAGTTTGC TGGAGCCGAC TACACAACTA CTGTGGAAAC TTTCATTGCT ACAAACGGAA GAGCAGTACA AGGAGGTACA AGCCATCACT TGGGACAAAA TTTCTCAAAG ATGTTCAAGA TTCAATTCGA AGCTGAAAAC AAGGAGACAC AATTTGCTTA CCAAAATTCA TGGGGCTTGT CAACTCGTAC TCTCGGTGTC ATGATTATGG TTCACGGAGA TGACAAGGGT ATGGTTTTAC CACCAAGAGT TGCTTTCTGC CAAGTTGTCG TTATTCCACT CATCTTCAAG GACAAGGATA ACGCCACTTT GGTAGAAAAG ACCAAGGAAA TTTATAATGA GCTTGAAAAG GCTGGAATTC GTGTCAAGTT GGATGATCGT TTGGAGAGAA CTCCAGGTTG GAAATACAAT TATTGGGAAC TCCGTGGTGT TCCATTGCGT ATTGAAGTTG GACCAAAGGA TTTGGAAAAG CAACAAATTA TGCTCTGTAG AAGAGACACA GGAGAAAAGT GGACCATGCC ACTCAGTGAA TTTTCTGGTG ATTCCATTAA GGCCGTTCTT GATAAAATTC ATGACTCCAT GTTGAACAAG GCCAGAAAGG AAATGAATGA AAGAATTGTA GTGACAAGAA CATGGCCAGA GTTCATCAAG GCACTCAACT CTGGAAATAT GTGTCTTATT CCTTGGCATG AAAGCAAGGC AGCAGAGGAA TATATTAAGG AAAAGTCAAA GTTGGAAAGT GTCCAAAGTC AATCTGATGC CAACACAGGT TTAACAGGAG CTGCCAAATC ATTGTGTGTC CCTCTCGACC AATCTTCCTT CCCATCATTG GAAGGTCTTG AGAACTTTTA TCCTGAAGAA GCTCACAAGA AGCCAAATTG TTGGGCTTTG TTCGGTAGAA GTTAT
Details for NafoA.18681.a.B2.GE40322
| HARVESTED ON: | 6/13/2016 |
| SEQUENCED ON: | 6/16/2016 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 60kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 50 |
Validated AA Sequence
MAHHHHHHMK QNEGIDVKKQ ENFSEWYSQV ITKSEFLDYY DVSGCYIFRP NCWFVWESVQ KFFDAEIKKL GVQNVMFPLF VTKRALETEK DHVEGFSPEV AWVTKSGNSD LQEPIALRPT SETIMYPSYA KWIQSHRDLP LKLNQWTNVV RWEFKHAVPF IRSREFYWQE GHSAFKSKEE ADEEVFTILE LYKRVYEELL AVPVIKGTKT ENEKFAGADY TTTVETFIAT NGRAVQGGTS HHLGQXXXKD VQDSIRS
Validated NT Sequence
acaatttggc ttcttgtgag cttcttcagg ataaaagttc tcaagacctt ccaatgatgg gaaggaagat tggtcgagag ggacacacaa tgatttggca gctcctgtta aacctgtgtt ggcatcagat tgactttgga cactttccaa ctttgacttt tccttaatat attcctctgc tgccttgctt tcatgccaag gaataagaca catatttcca gagttgagtg ccttgatgaa ctctggccat gttcttgtca ctacaattct ttcattcatt tcctttctgg ccttgttcaa catggagtca tgaattttat caagaacggc cttaatggaa tcaccagaaa attcactgag tggcatggtc cacttttctc ctgtgtctct tctacagagc ataatttgtt gcttttccaa atcctttggt ccaacttcaa tacgcaatgg aacaccacgg agttcccaat aattgtattt ccaacctgga gttctctcca aacgatcatc caacttgaca cgaattccag ccttttcaag ctcattataa atttccttgg tcttttctac caaagtggcg ttatccttgt ccttgaagat gagtggaata acgacaactt ggcagaaagc aactcttggt ggtaaaacca tacccttgtc atctccgtga accataatca tgacaccgag agtacgagtt gacaagcccc atgaattttg gtaagcaaat tgtgtctcct tgttttcagc ttcgaattga atcttgaaca tctttgnnnn nnttttgtcc caagtgatgg cttgtacctc cttgtactgc tcttccgttt gtagcaatga aagtttccac agtagttgtg tagtcggctc cagcaaactt ttcattttca gtcttggtac ccttgatgac tggaacagcc aacaattctt cgtaaactct tttatataac tccaaaatag taaaaacttc ttcatcagct tcttcctttg atttgaaagc actgtgacct tcttgccagt agaattctct ggatctaatg aaaggaacag catgtttaaa ttcccatcta acaacgtttg tccattggtt caattttaat ggcaaatctc tgtgagattg aatccacttg gcatacgatg gatacatgat ggtttcactt gttggtctca aagcaattgg ttcttgaaga tccgaattac cggacttggt gacccaagca acttctggag agaagccttc aacgtggtcc ttctcagttt ccaaagctct cttggtaaca aacaatggaa acatgacatt ttgaacaccc aactttttaa tttctgcatc aaagaacttt tgaacagact cccaaacaaa ccaacaattt ggtctgaaaa tataacatcc actcacatca tagtaatcca agaattcact cttggtgatg acttggctgt accattcgct aaagttttct tgcttcttga catcaatacc ttcgttttgt ttcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHKQ NEGIDVKKQE NFSEWYSQVI TKSEFLDYYD VSGCYIFRPN CWFVWESVQK FFDAEIKKLG VQNVMFPLFV TKRALETEKD HVEGFSPEVA WVTKSGNSDL QEPIALRPTS ETIMYPSYAK WIQSHRDLPL KLNQWTNVVR WEFKHAVPFI RSREFYWQEG HSAFKSKEEA DEEVFTILEL YKRVYEELLA VPVIKGTKTE NEKFAGADYT TTVETFIATN GRAVQGGTSH HLGQNFSKMF KIQFEAENKE TQFAYQNSWG LSTRTLGVMI MVHGDDKGMV LPPRVAFCQV VVIPLIFKDK DNATLVEKTK EIYNELEKAG IRVKLDDRLE RTPGWKYNYW ELRGVPLRIE VGPKDLEKQQ IMLCRRDTGE KWTMPLSEFS GDSIKAVLDK IHDSMLNKAR KEMNERIVVT RTWPEFIKAL NSGNMCLIPW HESKAAEEYI KEKSKLESVQ SQSDANTGLT GAAKSLCVPL DQSSFPSLEG LENFYPEEAH KKPNCWALFG RSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaacaaa acgaaggtat tgatgtcaag aagcaagaaa actttagcga atggtacagc caagtcatca ccaagagtga attcttggat tactatgatg tgagtggatg ttatattttc agaccaaatt gttggtttgt ttgggagtct gttcaaaagt tctttgatgc agaaattaaa aagttgggtg ttcaaaatgt catgtttcca ttgtttgtta ccaagagagc tttggaaact gagaaggacc acgttgaagg cttctctcca gaagttgctt gggtcaccaa gtccggtaat tcggatcttc aagaaccaat tgctttgaga ccaacaagtg aaaccatcat gtatccatcg tatgccaagt ggattcaatc tcacagagat ttgccattaa aattgaacca atggacaaac gttgttagat gggaatttaa acatgctgtt cctttcatta gatccagaga attctactgg caagaaggtc acagtgcttt caaatcaaag gaagaagctg atgaagaagt ttttactatt ttggagttat ataaaagagt ttacgaagaa ttgttggctg ttccagtcat caagggtacc aagactgaaa atgaaaagtt tgctggagcc gactacacaa ctactgtgga aactttcatt gctacaaacg gaagagcagt acaaggaggt acaagccatc acttgggaca aaatttctca aagatgttca agattcaatt cgaagctgaa aacaaggaga cacaatttgc ttaccaaaat tcatggggct tgtcaactcg tactctcggt gtcatgatta tggttcacgg agatgacaag ggtatggttt taccaccaag agttgctttc tgccaagttg tcgttattcc actcatcttc aaggacaagg ataacgccac tttggtagaa aagaccaagg aaatttataa tgagcttgaa aaggctggaa ttcgtgtcaa gttggatgat cgtttggaga gaactccagg ttggaaatac aattattggg aactccgtgg tgttccattg cgtattgaag ttggaccaaa ggatttggaa aagcaacaaa ttatgctctg tagaagagac acaggagaaa agtggaccat gccactcagt gaattttctg gtgattccat taaggccgtt cttgataaaa ttcatgactc catgttgaac aaggccagaa aggaaatgaa tgaaagaatt gtagtgacaa gaacatggcc agagttcatc aaggcactca actctggaaa tatgtgtctt attccttggc atgaaagcaa ggcagcagag gaatatatta aggaaaagtc aaagttggaa agtgtccaaa gtcaatctga tgccaacaca ggtttaacag gagctgccaa atcattgtgt gtccctctcg accaatcttc cttcccatca ttggaaggtc ttgagaactt ttatcctgaa gaagctcaca agaagccaaa ttgttgggct ttgttcggta gaagttattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for NafoA.18681.a.B3.GE42995
| HARVESTED ON: | 3/4/2019 |
| SEQUENCED ON: | 3/12/2019 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSG LKQNEGIDVK KQENFSEWYS QVITKSEFLD YYDVSGCYIF RPNCWFVWES VQKFFDAEIK KLGVQNVMFP LFVTKRALET EKDHVEGFSP EVAWVTKSGN SDLQEPIALR PTSETIMYPS YAKWIQSHRD LPLKLNQWTN VVRWEFKHAV PFIRSREFYW QEGHSAFKSK EEADEEVFTI LELYKRVYEE LLAVPVIKGT KTENEKFAGA DYTTTVETFI ATNGRAVQGG TSHHLGQNFS KMFKIQFEAE NKETQFAYQN SWGLSTRTLG VMIMVHGDDK GMVLPPRVAF CQVVVIPLIF KDKDNATLVE KTKEIYNELE KAGIRVKLDD RLERTPGWKY NYWELRGVPL RIEVGPKDLE KQQIMLCRRD TGEKWTMPLS EFSGDSIKAV LDKIHDSMLN KARKEMNERI VVTRTWPEFI KALNSGNMCL IPWHESKAAE EYIKEKSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctggtc tcaaacaaaa cgaaggtatt gatgtcaaga agcaagaaaa ctttagcgaa tggtacagcc aagtcatcac caagagtgaa ttcttggatt actatgatgt gagtggatgt tatattttca gaccaaattg ttggtttgtt tgggagtctg ttcaaaagtt ctttgatgca gaaattaaaa agttgggtgt tcaaaatgtc atgtttccat tgtttgttac caagagagct ttggaaactg agaaggacca cgttgaaggc ttctctccag aagttgcttg ggtcaccaag tccggtaatt cggatcttca agaaccaatt gctttgagac caacaagtga aaccatcatg tatccatcgt atgccaagtg gattcaatct cacagagatt tgccattaaa attgaaccaa tggacaaacg ttgttagatg ggaatttaaa catgctgttc ctttcattag atccagagaa ttctactggc aagaaggtca cagtgctttc aaatcaaagg aagaagctga tgaagaagtt tttactattt tggagttata taaaagagtt tacgaagaat tgttggctgt tccagtcatc aagggtacca agactgaaaa tgaaaagttt gctggagccg actacacaac tactgtggaa actttcattg ctacaaacgg aagagcagta caaggaggta caagccatca cttgggacaa aatttctcaa agatgttcaa gattcaattc gaagctgaaa acaaggagac acaatttgct taccaaaatt catggggctt gtcaactcgt actctcggtg tcatgattat ggttcacgga gatgacaagg gtatggtttt accaccaaga gttgctttct gccaagttgt cgttattcca ctcatcttca aggacaagga taacgccact ttggtagaaa agaccaagga aatttataat gagcttgaaa aggctggaat tcgtgtcaag ttggatgatc gtttggagag aactccaggt tggaaataca attattggga actccgtggt gttccattgc gtattgaagt tggaccaaag gatttggaaa agcaacaaat tatgctctgt agaagagaca caggagaaaa gtggaccatg ccactcagtg aattttctgg tgattccatt aaggccgttc ttgataaaat tcatgactcc atgttgaaca aggccagaaa ggaaatgaat gaaagaattg tagtgacaag aacatggcca gagttcatca aggcactcaa ctctggaaat atgtgtctta ttccttggca tgaaagcaag gcagcagagg aatatattaa ggaaaagtca aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.18681.a.B2.PW38555
| PURIFICATION DATe: | 10/29/2018 |
| CONCENTRATION: | 16.56mg/ml |
| OBSERVED MW: | 55kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1 mM ATP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 50 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK QNEGIDVKKQ ENFSEWYSQV ITKSEFLDYY DVSGCYIFRP NCWFVWESVQ KFFDAEIKKL GVQNVMFPLF VTKRALETEK DHVEGFSPEV AWVTKSGNSD LQEPIALRPT SETIMYPSYA KWIQSHRDLP LKLNQWTNVV RWEFKHAVPF IRSREFYWQE GHSAFKSKEE ADEEVFTILE LYKRVYEELL AVPVIKGTKT ENEKFAGADY TTTVETFIAT NGRAVQGGTS HHLGQXXXKD VQDSIRS
Validated NT Sequence
acaatttggc ttcttgtgag cttcttcagg ataaaagttc tcaagacctt ccaatgatgg gaaggaagat tggtcgagag ggacacacaa tgatttggca gctcctgtta aacctgtgtt ggcatcagat tgactttgga cactttccaa ctttgacttt tccttaatat attcctctgc tgccttgctt tcatgccaag gaataagaca catatttcca gagttgagtg ccttgatgaa ctctggccat gttcttgtca ctacaattct ttcattcatt tcctttctgg ccttgttcaa catggagtca tgaattttat caagaacggc cttaatggaa tcaccagaaa attcactgag tggcatggtc cacttttctc ctgtgtctct tctacagagc ataatttgtt gcttttccaa atcctttggt ccaacttcaa tacgcaatgg aacaccacgg agttcccaat aattgtattt ccaacctgga gttctctcca aacgatcatc caacttgaca cgaattccag ccttttcaag ctcattataa atttccttgg tcttttctac caaagtggcg ttatccttgt ccttgaagat gagtggaata acgacaactt ggcagaaagc aactcttggt ggtaaaacca tacccttgtc atctccgtga accataatca tgacaccgag agtacgagtt gacaagcccc atgaattttg gtaagcaaat tgtgtctcct tgttttcagc ttcgaattga atcttgaaca tctttgnnnn nnttttgtcc caagtgatgg cttgtacctc cttgtactgc tcttccgttt gtagcaatga aagtttccac agtagttgtg tagtcggctc cagcaaactt ttcattttca gtcttggtac ccttgatgac tggaacagcc aacaattctt cgtaaactct tttatataac tccaaaatag taaaaacttc ttcatcagct tcttcctttg atttgaaagc actgtgacct tcttgccagt agaattctct ggatctaatg aaaggaacag catgtttaaa ttcccatcta acaacgtttg tccattggtt caattttaat ggcaaatctc tgtgagattg aatccacttg gcatacgatg gatacatgat ggtttcactt gttggtctca aagcaattgg ttcttgaaga tccgaattac cggacttggt gacccaagca acttctggag agaagccttc aacgtggtcc ttctcagttt ccaaagctct cttggtaaca aacaatggaa acatgacatt ttgaacaccc aactttttaa tttctgcatc aaagaacttt tgaacagact cccaaacaaa ccaacaattt ggtctgaaaa tataacatcc actcacatca tagtaatcca agaattcact cttggtgatg acttggctgt accattcgct aaagttttct tgcttcttga catcaatacc ttcgttttgt ttcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHKQ NEGIDVKKQE NFSEWYSQVI TKSEFLDYYD VSGCYIFRPN CWFVWESVQK FFDAEIKKLG VQNVMFPLFV TKRALETEKD HVEGFSPEVA WVTKSGNSDL QEPIALRPTS ETIMYPSYAK WIQSHRDLPL KLNQWTNVVR WEFKHAVPFI RSREFYWQEG HSAFKSKEEA DEEVFTILEL YKRVYEELLA VPVIKGTKTE NEKFAGADYT TTVETFIATN GRAVQGGTSH HLGQNFSKMF KIQFEAENKE TQFAYQNSWG LSTRTLGVMI MVHGDDKGMV LPPRVAFCQV VVIPLIFKDK DNATLVEKTK EIYNELEKAG IRVKLDDRLE RTPGWKYNYW ELRGVPLRIE VGPKDLEKQQ IMLCRRDTGE KWTMPLSEFS GDSIKAVLDK IHDSMLNKAR KEMNERIVVT RTWPEFIKAL NSGNMCLIPW HESKAAEEYI KEKSKLESVQ SQSDANTGLT GAAKSLCVPL DQSSFPSLEG LENFYPEEAH KKPNCWALFG RSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAAACAAA ACGAAGGTAT TGATGTCAAG AAGCAAGAAA ACTTTAGCGA ATGGTACAGC CAAGTCATCA CCAAGAGTGA ATTCTTGGAT TACTATGATG TGAGTGGATG TTATATTTTC AGACCAAATT GTTGGTTTGT TTGGGAGTCT GTTCAAAAGT TCTTTGATGC AGAAATTAAA AAGTTGGGTG TTCAAAATGT CATGTTTCCA TTGTTTGTTA CCAAGAGAGC TTTGGAAACT GAGAAGGACC ACGTTGAAGG CTTCTCTCCA GAAGTTGCTT GGGTCACCAA GTCCGGTAAT TCGGATCTTC AAGAACCAAT TGCTTTGAGA CCAACAAGTG AAACCATCAT GTATCCATCG TATGCCAAGT GGATTCAATC TCACAGAGAT TTGCCATTAA AATTGAACCA ATGGACAAAC GTTGTTAGAT GGGAATTTAA ACATGCTGTT CCTTTCATTA GATCCAGAGA ATTCTACTGG CAAGAAGGTC ACAGTGCTTT CAAATCAAAG GAAGAAGCTG ATGAAGAAGT TTTTACTATT TTGGAGTTAT ATAAAAGAGT TTACGAAGAA TTGTTGGCTG TTCCAGTCAT CAAGGGTACC AAGACTGAAA ATGAAAAGTT TGCTGGAGCC GACTACACAA CTACTGTGGA AACTTTCATT GCTACAAACG GAAGAGCAGT ACAAGGAGGT ACAAGCCATC ACTTGGGACA AAATTTCTCA AAGATGTTCA AGATTCAATT CGAAGCTGAA AACAAGGAGA CACAATTTGC TTACCAAAAT TCATGGGGCT TGTCAACTCG TACTCTCGGT GTCATGATTA TGGTTCACGG AGATGACAAG GGTATGGTTT TACCACCAAG AGTTGCTTTC TGCCAAGTTG TCGTTATTCC ACTCATCTTC AAGGACAAGG ATAACGCCAC TTTGGTAGAA AAGACCAAGG AAATTTATAA TGAGCTTGAA AAGGCTGGAA TTCGTGTCAA GTTGGATGAT CGTTTGGAGA GAACTCCAGG TTGGAAATAC AATTATTGGG AACTCCGTGG TGTTCCATTG CGTATTGAAG TTGGACCAAA GGATTTGGAA AAGCAACAAA TTATGCTCTG TAGAAGAGAC ACAGGAGAAA AGTGGACCAT GCCACTCAGT GAATTTTCTG GTGATTCCAT TAAGGCCGTT CTTGATAAAA TTCATGACTC CATGTTGAAC AAGGCCAGAA AGGAAATGAA TGAAAGAATT GTAGTGACAA GAACATGGCC AGAGTTCATC AAGGCACTCA ACTCTGGAAA TATGTGTCTT ATTCCTTGGC ATGAAAGCAA GGCAGCAGAG GAATATATTA AGGAAAAGTC AAAGTTGGAA AGTGTCCAAA GTCAATCTGA TGCCAACACA GGTTTAACAG GAGCTGCCAA ATCATTGTGT GTCCCTCTCG ACCAATCTTC CTTCCCATCA TTGGAAGGTC TTGAGAACTT TTATCCTGAA GAAGCTCACA AGAAGCCAAA TTGTTGGGCT TTGTTCGGTA GAAGTTATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for NafoA.18681.a.B2.PW38711
| PURIFICATION DATe: | 9/30/2019 |
| CONCENTRATION: | 87.9mg/ml |
| OBSERVED MW: | 60kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide, 1 mM ATP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 50 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK QNEGIDVKKQ ENFSEWYSQV ITKSEFLDYY DVSGCYIFRP NCWFVWESVQ KFFDAEIKKL GVQNVMFPLF VTKRALETEK DHVEGFSPEV AWVTKSGNSD LQEPIALRPT SETIMYPSYA KWIQSHRDLP LKLNQWTNVV RWEFKHAVPF IRSREFYWQE GHSAFKSKEE ADEEVFTILE LYKRVYEELL AVPVIKGTKT ENEKFAGADY TTTVETFIAT NGRAVQGGTS HHLGQXXXKD VQDSIRS
Validated NT Sequence
acaatttggc ttcttgtgag cttcttcagg ataaaagttc tcaagacctt ccaatgatgg gaaggaagat tggtcgagag ggacacacaa tgatttggca gctcctgtta aacctgtgtt ggcatcagat tgactttgga cactttccaa ctttgacttt tccttaatat attcctctgc tgccttgctt tcatgccaag gaataagaca catatttcca gagttgagtg ccttgatgaa ctctggccat gttcttgtca ctacaattct ttcattcatt tcctttctgg ccttgttcaa catggagtca tgaattttat caagaacggc cttaatggaa tcaccagaaa attcactgag tggcatggtc cacttttctc ctgtgtctct tctacagagc ataatttgtt gcttttccaa atcctttggt ccaacttcaa tacgcaatgg aacaccacgg agttcccaat aattgtattt ccaacctgga gttctctcca aacgatcatc caacttgaca cgaattccag ccttttcaag ctcattataa atttccttgg tcttttctac caaagtggcg ttatccttgt ccttgaagat gagtggaata acgacaactt ggcagaaagc aactcttggt ggtaaaacca tacccttgtc atctccgtga accataatca tgacaccgag agtacgagtt gacaagcccc atgaattttg gtaagcaaat tgtgtctcct tgttttcagc ttcgaattga atcttgaaca tctttgnnnn nnttttgtcc caagtgatgg cttgtacctc cttgtactgc tcttccgttt gtagcaatga aagtttccac agtagttgtg tagtcggctc cagcaaactt ttcattttca gtcttggtac ccttgatgac tggaacagcc aacaattctt cgtaaactct tttatataac tccaaaatag taaaaacttc ttcatcagct tcttcctttg atttgaaagc actgtgacct tcttgccagt agaattctct ggatctaatg aaaggaacag catgtttaaa ttcccatcta acaacgtttg tccattggtt caattttaat ggcaaatctc tgtgagattg aatccacttg gcatacgatg gatacatgat ggtttcactt gttggtctca aagcaattgg ttcttgaaga tccgaattac cggacttggt gacccaagca acttctggag agaagccttc aacgtggtcc ttctcagttt ccaaagctct cttggtaaca aacaatggaa acatgacatt ttgaacaccc aactttttaa tttctgcatc aaagaacttt tgaacagact cccaaacaaa ccaacaattt ggtctgaaaa tataacatcc actcacatca tagtaatcca agaattcact cttggtgatg acttggctgt accattcgct aaagttttct tgcttcttga catcaatacc ttcgttttgt ttcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHKQ NEGIDVKKQE NFSEWYSQVI TKSEFLDYYD VSGCYIFRPN CWFVWESVQK FFDAEIKKLG VQNVMFPLFV TKRALETEKD HVEGFSPEVA WVTKSGNSDL QEPIALRPTS ETIMYPSYAK WIQSHRDLPL KLNQWTNVVR WEFKHAVPFI RSREFYWQEG HSAFKSKEEA DEEVFTILEL YKRVYEELLA VPVIKGTKTE NEKFAGADYT TTVETFIATN GRAVQGGTSH HLGQNFSKMF KIQFEAENKE TQFAYQNSWG LSTRTLGVMI MVHGDDKGMV LPPRVAFCQV VVIPLIFKDK DNATLVEKTK EIYNELEKAG IRVKLDDRLE RTPGWKYNYW ELRGVPLRIE VGPKDLEKQQ IMLCRRDTGE KWTMPLSEFS GDSIKAVLDK IHDSMLNKAR KEMNERIVVT RTWPEFIKAL NSGNMCLIPW HESKAAEEYI KEKSKLESVQ SQSDANTGLT GAAKSLCVPL DQSSFPSLEG LENFYPEEAH KKPNCWALFG RSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAAACAAA ACGAAGGTAT TGATGTCAAG AAGCAAGAAA ACTTTAGCGA ATGGTACAGC CAAGTCATCA CCAAGAGTGA ATTCTTGGAT TACTATGATG TGAGTGGATG TTATATTTTC AGACCAAATT GTTGGTTTGT TTGGGAGTCT GTTCAAAAGT TCTTTGATGC AGAAATTAAA AAGTTGGGTG TTCAAAATGT CATGTTTCCA TTGTTTGTTA CCAAGAGAGC TTTGGAAACT GAGAAGGACC ACGTTGAAGG CTTCTCTCCA GAAGTTGCTT GGGTCACCAA GTCCGGTAAT TCGGATCTTC AAGAACCAAT TGCTTTGAGA CCAACAAGTG AAACCATCAT GTATCCATCG TATGCCAAGT GGATTCAATC TCACAGAGAT TTGCCATTAA AATTGAACCA ATGGACAAAC GTTGTTAGAT GGGAATTTAA ACATGCTGTT CCTTTCATTA GATCCAGAGA ATTCTACTGG CAAGAAGGTC ACAGTGCTTT CAAATCAAAG GAAGAAGCTG ATGAAGAAGT TTTTACTATT TTGGAGTTAT ATAAAAGAGT TTACGAAGAA TTGTTGGCTG TTCCAGTCAT CAAGGGTACC AAGACTGAAA ATGAAAAGTT TGCTGGAGCC GACTACACAA CTACTGTGGA AACTTTCATT GCTACAAACG GAAGAGCAGT ACAAGGAGGT ACAAGCCATC ACTTGGGACA AAATTTCTCA AAGATGTTCA AGATTCAATT CGAAGCTGAA AACAAGGAGA CACAATTTGC TTACCAAAAT TCATGGGGCT TGTCAACTCG TACTCTCGGT GTCATGATTA TGGTTCACGG AGATGACAAG GGTATGGTTT TACCACCAAG AGTTGCTTTC TGCCAAGTTG TCGTTATTCC ACTCATCTTC AAGGACAAGG ATAACGCCAC TTTGGTAGAA AAGACCAAGG AAATTTATAA TGAGCTTGAA AAGGCTGGAA TTCGTGTCAA GTTGGATGAT CGTTTGGAGA GAACTCCAGG TTGGAAATAC AATTATTGGG AACTCCGTGG TGTTCCATTG CGTATTGAAG TTGGACCAAA GGATTTGGAA AAGCAACAAA TTATGCTCTG TAGAAGAGAC ACAGGAGAAA AGTGGACCAT GCCACTCAGT GAATTTTCTG GTGATTCCAT TAAGGCCGTT CTTGATAAAA TTCATGACTC CATGTTGAAC AAGGCCAGAA AGGAAATGAA TGAAAGAATT GTAGTGACAA GAACATGGCC AGAGTTCATC AAGGCACTCA ACTCTGGAAA TATGTGTCTT ATTCCTTGGC ATGAAAGCAA GGCAGCAGAG GAATATATTA AGGAAAAGTC AAAGTTGGAA AGTGTCCAAA GTCAATCTGA TGCCAACACA GGTTTAACAG GAGCTGCCAA ATCATTGTGT GTCCCTCTCG ACCAATCTTC CTTCCCATCA TTGGAAGGTC TTGAGAACTT TTATCCTGAA GAAGCTCACA AGAAGCCAAA TTGTTGGGCT TTGTTCGGTA GAAGTTATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT