SSGCID: Target Page

NafoA.18331.a

fumarate hydratase

CENTER ID:	NafoA.18331.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.18331.a.B1.GE40631	Full length(NafoA.18331.a)		1	584

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0114270

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRSQSSAKT FLRSLGVVQN KSLSSLLMMR AGSTSSSCYS TGPTFKYEDL FQVGEDETEY RLITKDYVSS LKVRDEKTGE TREILKVDPQ GLTAITQAAI SDIQYLLRPS HLKQLSNILK DPEASDNDRF VAKTLLKNAV IASGRKLPGC QDTGTAIVMG KKGQYVWTDG QDEKHISEGV FKTYTQQYLR YSQVAPLTMY EEKNTGCNLP AQIDLFATDG DEYKFLFIAK GGGSANKTYL YQQTKALLNP NSLMKFLEEK IKSIGTSACP PYHLAIVIGG LSAELTLKTV KLASTKYYDE LPTTGSPGGR AFRDVELEKE ILKLTQKMGI GAQFGGKYFC HDVRVIRLPR HGASCPVGIG VSCSADRQVK GKITKDGIFL EQLETDPAQY LPEVEEKDLS EHVVEIDLNR PMKEILSILS KYPIKTRLSL SGPLIVARDI AHAKLKERLD RGEGLPDYIK NHIIYYAGPA KTPEGYASGS FGPTTAGRMD SYVDLFQQNG GSLITLAKGN RSREVTQACS KYGGFYLGSI GGPAAILAAN NIKKVEVLEY PELGMEAIWK IEVKDFPAFI VVDDKGNDFF KELQ

NT Sequence

ATGCTTCGAA GCCAATCCTC AGCAAAGACA TTCCTCCGTT CTCTTGGCGT GGTTCAAAAC AAATCCTTGT CATCCTTGTT GATGATGAGA GCTGGTTCAA CATCATCATC GTGTTATTCT ACAGGACCTA CGTTCAAGTA CGAGGATTTA TTCCAAGTTG GAGAGGATGA AACCGAATAC CGATTAATTA CTAAAGATTA CGTTTCATCT CTTAAAGTGA GAGATGAAAA GACTGGAGAG ACTAGAGAAA TTTTGAAGGT AGATCCACAA GGATTAACCG CAATTACTCA GGCTGCCATT TCCGATATTC AATACTTGTT GCGCCCATCT CACTTGAAAC AACTTTCAAA CATTTTGAAG GATCCTGAAG CTTCCGACAA TGATCGTTTT GTTGCCAAGA CTCTTTTGAA GAATGCTGTG ATTGCTTCTG GAAGAAAGCT TCCTGGTTGC CAAGATACTG GTACTGCAAT TGTTATGGGC AAGAAGGGAC AATATGTTTG GACCGATGGA CAGGATGAAA AGCACATCTC TGAAGGAGTA TTCAAGACAT ATACTCAGCA ATATTTACGT TATTCACAAG TCGCACCACT CACCATGTAT GAGGAGAAGA ATACTGGTTG CAACTTGCCT GCACAAATCG ACTTGTTTGC CACCGATGGT GACGAGTACA AGTTCTTGTT TATTGCAAAG GGAGGTGGTA GTGCTAACAA GACCTACTTG TATCAACAAA CTAAGGCATT GTTGAATCCA AATTCATTGA TGAAGTTTTT GGAGGAAAAA ATTAAATCAA TCGGTACCTC TGCTTGTCCA CCATACCACT TGGCAATTGT TATTGGAGGT TTGAGTGCAG AACTTACTTT GAAGACTGTG AAATTGGCAA GTACCAAATA CTATGATGAA TTGCCAACAA CAGGAAGCCC AGGAGGTAGA GCATTCCGTG ATGTTGAATT GGAGAAGGAA ATTTTGAAGC TCACTCAAAA GATGGGTATT GGAGCTCAAT TTGGAGGAAA ATATTTCTGT CACGATGTCA GAGTGATCCG TTTACCACGT CATGGTGCCT CTTGCCCAGT GGGTATTGGT GTTTCCTGTT CTGCTGACAG ACAAGTGAAG GGTAAAATTA CTAAGGATGG AATCTTTTTG GAACAATTAG AAACTGACCC AGCTCAATAT CTCCCAGAGG TTGAAGAAAA GGATTTGAGC GAACATGTGG TAGAAATTGA TTTGAACAGA CCAATGAAAG AAATTTTGAG CATTCTCTCC AAATATCCAA TCAAGACTCG TCTCTCATTG AGTGGTCCAC TCATTGTGGC TCGTGACATT GCTCATGCCA AATTGAAGGA GCGTCTTGAC CGAGGTGAAG GTTTACCTGA TTACATTAAG AATCACATTA TTTATTATGC TGGTCCTGCT AAGACTCCAG AAGGTTATGC TTCAGGTTCT TTCGGACCAA CAACTGCAGG TAGAATGGAC TCCTATGTTG ATTTATTCCA ACAAAATGGT GGAAGTTTGA TCACACTTGC TAAGGGTAAC AGAAGTCGTG AGGTGACACA AGCTTGTTCC AAGTATGGAG GATTTTATTT AGGCTCGATT GGTGGTCCAG CTGCAATTTT GGCTGCTAAT AACATCAAGA AGGTTGAAGT TTTGGAATAT CCAGAGTTAG GAATGGAGGC AATTTGGAAG ATTGAAGTCA AGGACTTCCC TGCTTTCATT GTGGTTGACG ATAAAGGAAA CGACTTTTTC AAGGAATTGC AA

Details for NafoA.18331.a.B1.GE40631

HARVESTED ON:	10/18/2016
SEQUENCED ON:	10/19/2016
EXPECTED MW:	65kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	43

Validated AA Sequence

Validated NT Sequence

ttcctttatc gtcaaccaca atgaaagcag ggaagtcctt gacttcaatc ttccaaattg cctccattcc taactctgga tattccaaaa cttcaacctt cttgatgtta ttagcagcca aaattgcagc tggaccacca atcgagccta aataaaatcc tccatacttg gaacaagctt gtgtcacctc acgacttctg ttacccttag caagtgtgat caaacttcca ccattttgtt ggaataaatc aacataggag tccattctac ctgcagttgt tggtccgaaa gaacctgaag cataaccttc tggagtctta gcaggaccag cataataaat aatgtgattc ttaatgtaat caggtaaacc ttcacctcgg tcaagacgct ccttcaattt ggcatgagca atgtcacgag ccacaatgag tggaccactc aatgagagac gagtcttgat tggatatttg gagagaatgc tcaaaatttc tttcattggt ctgttcaaat caatttctac cacatgttcg ctcaaatcct tttcttcaac ctctgggaga tattgagctg ggtcagtttc taattgttcc aaaaagattc catccttagt aattttaccc ttcacttgtc tgtcagcaga acaggaaaca ccaataccca ctgggcaaga ggcaccatga cgtggtaaac ggatcactct gacatcgtga cagaaatatt ttcctcnnnn nnnngctcca atacccatct tttgagtgag cttcaaaatt tccttctnnn nntcaacatc acggaatgct ctnnnnnctg ggcttcctgt tgttnnnnnn catcatnnnn nnnnnnnnnn nnnatttcnn ngtcttcnnn nnnnnnnnnn nnntcannnn nnnnataaca nnnnncaagt ggtatggtgg acaagcagag gtaccgattg atttannnnn nncctccaaa aannncatca ntnaatttgn nnnnnacaan gccttagttn gtgatacaag taggtcttgt tagcactacc acctcccttt gcaataaaca agaacttgta ctcgtcacca tcggtggcaa acaagtcgat ttgtgcaggc aagttgcaac cagtattctt ctcctcatac atggtgagtg gtgcgacttg tgaataacgt aaatattgct gagtatatgt cttgaatact ccttcagaga tgtgcttttc atcctgtcca tcggtccaaa catattgtcc cttcttgccc ataacaattg cagtaccagt atcttggcaa ccaggaagct ttcttccaga agcaatcaca gcattcttca aaagagtctt ggcaacaaaa cgatcattgt cggaagcttc aggatccttc aaaatgtttg aaagttgttt caagtgagat gggcgcaaca agtattgaat atcggaaatg gcagcctgag taattgcggt taatccttgt ggatctacct tcaaaatttc tctagtctct ccagtctttt catctctcac tttaagagat gaaacgtaat ctttagtaat taatcggtat tcggtttcat cctctccaac ttggaataaa tcctcgtact tgaacgtagg tcctgtagaa taacacgatg atgatgttga accagctctc atcatcaaca aggatgacaa ggatttgttt tgaaccacgc caagagaacg gaggaatgtc tttgctgagg attggcttcg aagcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHML RSQSSAKTFL RSLGVVQNKS LSSLLMMRAG STSSSCYSTG PTFKYEDLFQ VGEDETEYRL ITKDYVSSLK VRDEKTGETR EILKVDPQGL TAITQAAISD IQYLLRPSHL KQLSNILKDP EASDNDRFVA KTLLKNAVIA SGRKLPGCQD TGTAIVMGKK GQYVWTDGQD EKHISEGVFK TYTQQYLRYS QVAPLTMYEE KNTGCNLPAQ IDLFATDGDE YKFLFIAKGG GSANKTYLYQ QTKALLNPNS LMKFLEEKIK SIGTSACPPY HLAIVIGGLS AELTLKTVKL ASTKYYDELP TTGSPGGRAF RDVELEKEIL KLTQKMGIGA QFGGKYFCHD VRVIRLPRHG ASCPVGIGVS CSADRQVKGK ITKDGIFLEQ LETDPAQYLP EVEEKDLSEH VVEIDLNRPM KEILSILSKY PIKTRLSLSG PLIVARDIAH AKLKERLDRG EGLPDYIKNH IIYYAGPAKT PEGYASGSFG PTTAGRMDSY VDLFQQNGGS LITLAKGNRS REVTQACSKY GGFYLGSIGG PAAILAANNI KKVEVLEYPE LGMEAIWKIE VKDFPAFIVV DDKGNDFFKE LQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc gaagccaatc ctcagcaaag acattcctcc gttctcttgg cgtggttcaa aacaaatcct tgtcatcctt gttgatgatg agagctggtt caacatcatc atcgtgttat tctacaggac ctacgttcaa gtacgaggat ttattccaag ttggagagga tgaaaccgaa taccgattaa ttactaaaga ttacgtttca tctcttaaag tgagagatga aaagactgga gagactagag aaattttgaa ggtagatcca caaggattaa ccgcaattac tcaggctgcc atttccgata ttcaatactt gttgcgccca tctcacttga aacaactttc aaacattttg aaggatcctg aagcttccga caatgatcgt tttgttgcca agactctttt gaagaatgct gtgattgctt ctggaagaaa gcttcctggt tgccaagata ctggtactgc aattgttatg ggcaagaagg gacaatatgt ttggaccgat ggacaggatg aaaagcacat ctctgaagga gtattcaaga catatactca gcaatattta cgttattcac aagtcgcacc actcaccatg tatgaggaga agaatactgg ttgcaacttg cctgcacaaa tcgacttgtt tgccaccgat ggtgacgagt acaagttctt gtttattgca aagggaggtg gtagtgctaa caagacctac ttgtatcaac aaactaaggc attgttgaat ccaaattcat tgatgaagtt tttggaggaa aaaattaaat caatcggtac ctctgcttgt ccaccatacc acttggcaat tgttattgga ggtttgagtg cagaacttac tttgaagact gtgaaattgg caagtaccaa atactatgat gaattgccaa caacaggaag cccaggaggt agagcattcc gtgatgttga attggagaag gaaattttga agctcactca aaagatgggt attggagctc aatttggagg aaaatatttc tgtcacgatg tcagagtgat ccgtttacca cgtcatggtg cctcttgccc agtgggtatt ggtgtttcct gttctgctga cagacaagtg aagggtaaaa ttactaagga tggaatcttt ttggaacaat tagaaactga cccagctcaa tatctcccag aggttgaaga aaaggatttg agcgaacatg tggtagaaat tgatttgaac agaccaatga aagaaatttt gagcattctc tccaaatatc caatcaagac tcgtctctca ttgagtggtc cactcattgt ggctcgtgac attgctcatg ccaaattgaa ggagcgtctt gaccgaggtg aaggtttacc tgattacatt aagaatcaca ttatttatta tgctggtcct gctaagactc cagaaggtta tgcttcaggt tctttcggac caacaactgc aggtagaatg gactcctatg ttgatttatt ccaacaaaat ggtggaagtt tgatcacact tgctaagggt aacagaagtc gtgaggtgac acaagcttgt tccaagtatg gaggatttta tttaggctcg attggtggtc cagctgcaat tttggctgct aataacatca agaaggttga agttttggaa tatccagagt taggaatgga ggcaatttgg aagattgaag tcaaggactt ccctgctttc attgtggttg acgataaagg aaacgacttt ttcaaggaat tgcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat