SSGCID: Target Page

NafoA.18292.a

cct chaperonin gamma subunit

CENTER ID:	NafoA.18292.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.18292.a.B1.GE40630	Full length(NafoA.18292.a)		1	536

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0016970

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFAQARPQIF VLKGNSKREQ GSKAQLANIQ AAKAIASIVR TTLGPKAMLK MILDQMGTVQ LTSDGNAILR ECDVTHPAAK SMIELSRTQD EEVGDGTTSV IILAGEILTV AEPLLERKIH PTRIVKGFLK ALEDAVNFIS EKMSRTIDTN NYEQVAEIVN TSIGTKFTHR YSDLMCKLAI DAVRTVAIDL ENGKKDIDVK RYARIEKIPG GLLSESRVLS GVMINKDILH PKMRRRIENP RILLLDCPLE YKKGESETNV VLEKEEDFET LLKMEDEWIK KTCGDIIKFK PDLVITEKGC SDLAQYYLLK HNISALRRVR KTDNNRIARA TGATIVSRTS EIRESDIGTG AGLFEVRKIG DEYFSFIENC KDPKACTILL RGGSKDILNE IERNLEDALH VVKNIFIDPR IVPGGGAVEM SVSQHLMQKS KSIKGIEQLP YQAVASSLEV IPRTLAENCG ANTIRLITEL RAKHANEQNL TLGIDGQKGV IADTAELKIW EPYEVKVQTI KTAVEAACLI LRIDDVLSSL KPKEKK

NT Sequence

ATGTTCGCAC AAGCTAGACC TCAAATCTTT GTACTCAAGG GTAACTCGAA GAGAGAGCAA GGTTCAAAGG CTCAACTTGC CAATATCCAA GCTGCTAAAG CCATTGCCTC CATTGTGAGA ACCACTCTCG GACCAAAGGC CATGCTCAAA ATGATTCTCG ATCAGATGGG TACTGTTCAA CTGACCAGTG ATGGTAATGC CATCCTCAGA GAATGCGACG TTACTCATCC AGCAGCTAAG AGTATGATTG AATTGAGTAG AACACAAGAT GAAGAAGTTG GTGATGGTAC CACTTCAGTC ATCATCCTTG CCGGTGAAAT TCTTACAGTG GCCGAGCCAT TGTTGGAAAG AAAGATTCAC CCAACTCGTA TTGTGAAAGG CTTTTTGAAA GCTCTTGAGG ATGCTGTTAA CTTTATTTCT GAAAAGATGT CAAGAACTAT CGATACTAAC AATTATGAAC AAGTAGCAGA AATTGTGAAT ACTAGTATTG GTACTAAATT CACTCATCGT TACAGCGATT TGATGTGTAA ATTGGCAATT GATGCTGTGC GAACAGTTGC TATCGATTTG GAGAATGGAA AGAAAGATAT CGATGTTAAA AGATATGCAC GTATCGAAAA GATTCCAGGT GGATTATTAT CTGAATCTCG AGTATTGAGC GGTGTCATGA TTAACAAGGA TATTCTTCAT CCAAAGATGA GAAGAAGAAT TGAAAATCCA AGAATTTTAC TTTTGGACTG TCCTCTCGAA TACAAGAAGG GTGAAAGCGA AACCAATGTT GTTTTGGAAA AGGAAGAAGA TTTCGAAACC CTACTCAAGA TGGAAGACGA GTGGATTAAG AAGACTTGTG GCGACATTAT CAAATTCAAA CCAGATCTTG TCATTACTGA AAAGGGATGC TCGGATTTGG CCCAATATTA TCTTCTGAAA CATAATATTT CTGCTCTCCG TCGCGTCAGA AAGACGGACA ACAACCGTAT TGCTCGTGCT ACAGGAGCCA CAATCGTTTC GAGAACATCT GAAATCAGAG AATCTGATAT CGGAACAGGA GCTGGTTTAT TTGAAGTTAG AAAGATTGGT GACGAATACT TTTCATTCAT TGAAAATTGC AAAGATCCTA AGGCATGCAC AATTCTTTTG AGAGGTGGAA GCAAGGATAT TTTGAATGAA ATTGAAAGAA ATCTGGAGGA TGCTTTGCAT GTTGTAAAGA ATATCTTTAT TGATCCTCGT ATTGTTCCTG GAGGTGGTGC TGTTGAAATG AGCGTTTCTC AACATTTGAT GCAAAAATCA AAAAGTATCA AGGGAATTGA ACAACTTCCA TACCAAGCTG TTGCTTCTTC ATTAGAGGTT ATTCCTAGAA CGTTGGCAGA AAACTGTGGA GCTAATACAA TTCGTCTCAT CACTGAATTG AGAGCTAAAC ACGCCAATGA ACAAAATCTT ACTTTGGGAA TTGATGGCCA AAAGGGTGTT ATTGCTGACA CTGCAGAATT GAAGATTTGG GAACCTTACG AAGTGAAGGT TCAAACCATT AAGACCGCTG TTGAGGCAGC TTGTTTGATT CTTCGTATTG ACGACGTTTT GTCCTCATTA AAGCCTAAGG AAAAGAAA

Details for NafoA.18292.a.B1.GE40630

HARVESTED ON:	10/18/2016
SEQUENCED ON:	10/19/2016
EXPECTED MW:	61kDa
OBSERVED MW:	61kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	95
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

atgaggacaa aacgtcgtca atacgaagaa tcaaacaagc tgcctcaaca gcggtcttaa tggtttgaac cttcacttcg taaggttccc aaatcttcaa ttctgcagtg tcagcaataa cacccttttg gccatcaatt cccaaagtaa gattttgttc attggcgtgt ttagctctca attcagtgat gagacgaatt gtattagctc cacagttttc tgccaacgtt ctaggaataa cctctaatgc agaagcaaca gcttggtatg gaagttgttc aattcccttg atactttttg atttttgcat caaatgttga gaaacgctca tttcaacagc accacctcca ggaacaatac gaggatcaat aaagatattc tttacaacat gcaaagcatc ctccagattt ctttcaattt cattcaaaat atccttgctt ccacctctca aaagaattgt gcatgcctta ggatctttgc aattttcaat gaatgaaaag tattcgtcac caatctttct aacttcaaat aaaccagctc ctgttccgat atcagattct ctgatttcag atgttctcga aacgattgtg gctcctgtag cacgagcaat acggttgttg tccgtctttc tgacgcgacg gagagcagaa atattatgtt tcagaagata atattgggcc aaatccgagc atcccttttc agtaatgaca agatctggtt tgaatttgat aatgtcgcca caagtcttct taatccactc gtcttccatc ttgagtaggg tttcgaaatc ttcttccttt tccaaaacaa cattgggttt cgctttcacc cttcttgtat tcgagaggac agtccaaaag taaaattctt ggattttcaa ttcttcttct catctttgga tgaagaatat ccttgttaat catgacaccg ctcaatactc gagattcaga taataatcca cctggaatct tttcgatacg tgcatatctt ttaacatcga tatctttctt tccattctcc aaatcgatag caactgttcg cacagcatca attgccaatt tacacatcaa atcgctgtaa cgatgagtga atttagtacc aatactagta ttcacaattt ctgctacttg ttcataattg ttagtatcga tagttcttga catcttttca gaaataaagt taacagcatc ctcaagagct ttcaaaaagc ctttcacaat acgagttggg tgaatctttc tttccaacaa tggctcggcc actgtaagaa tttcaccggc aaggatgatg actgaagtgg taccatcacc aacttcttca tcttgtgttc tactcaattc aatcatactc ttagctgctg gatgagtaac gtcgcattct ctgaggatgg cattaccatc actggtcagt tgaacagtac ccatctgatc gagaatcatt ttgagcatgg cctttggtcc gagagtggtt ctcacaatgg aggcaatggc tttagcagct tggatattgg caagttgagc ctttgaacct tgctctctct tcgagttacc cttgagtaca aagatttgag gtctagcttg tgcgaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMF AQARPQIFVL KGNSKREQGS KAQLANIQAA KAIASIVRTT LGPKAMLKMI LDQMGTVQLT SDGNAILREC DVTHPAAKSM IELSRTQDEE VGDGTTSVII LAGEILTVAE PLLERKIHPT RIVKGFLKAL EDAVNFISEK MSRTIDTNNY EQVAEIVNTS IGTKFTHRYS DLMCKLAIDA VRTVAIDLEN GKKDIDVKRY ARIEKIPGGL LSESRVLSGV MINKDILHPK MRRRIENPRI LLLDCPLEYK KGESETNVVL EKEEDFETLL KMEDEWIKKT CGDIIKFKPD LVITEKGCSD LAQYYLLKHN ISALRRVRKT DNNRIARATG ATIVSRTSEI RESDIGTGAG LFEVRKIGDE YFSFIENCKD PKACTILLRG GSKDILNEIE RNLEDALHVV KNIFIDPRIV PGGGAVEMSV SQHLMQKSKS IKGIEQLPYQ AVASSLEVIP RTLAENCGAN TIRLITELRA KHANEQNLTL GIDGQKGVIA DTAELKIWEP YEVKVQTIKT AVEAACLILR IDDVLSSLKP KEKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcg cacaagctag acctcaaatc tttgtactca agggtaactc gaagagagag caaggttcaa aggctcaact tgccaatatc caagctgcta aagccattgc ctccattgtg agaaccactc tcggaccaaa ggccatgctc aaaatgattc tcgatcagat gggtactgtt caactgacca gtgatggtaa tgccatcctc agagaatgcg acgttactca tccagcagct aagagtatga ttgaattgag tagaacacaa gatgaagaag ttggtgatgg taccacttca gtcatcatcc ttgccggtga aattcttaca gtggccgagc cattgttgga aagaaagatt cacccaactc gtattgtgaa aggctttttg aaagctcttg aggatgctgt taactttatt tctgaaaaga tgtcaagaac tatcgatact aacaattatg aacaagtagc agaaattgtg aatactagta ttggtactaa attcactcat cgttacagcg atttgatgtg taaattggca attgatgctg tgcgaacagt tgctatcgat ttggagaatg gaaagaaaga tatcgatgtt aaaagatatg cacgtatcga aaagattcca ggtggattat tatctgaatc tcgagtattg agcggtgtca tgattaacaa ggatattctt catccaaaga tgagaagaag aattgaaaat ccaagaattt tacttttgga ctgtcctctc gaatacaaga agggtgaaag cgaaaccaat gttgttttgg aaaaggaaga agatttcgaa accctactca agatggaaga cgagtggatt aagaagactt gtggcgacat tatcaaattc aaaccagatc ttgtcattac tgaaaaggga tgctcggatt tggcccaata ttatcttctg aaacataata tttctgctct ccgtcgcgtc agaaagacgg acaacaaccg tattgctcgt gctacaggag ccacaatcgt ttcgagaaca tctgaaatca gagaatctga tatcggaaca ggagctggtt tatttgaagt tagaaagatt ggtgacgaat acttttcatt cattgaaaat tgcaaagatc ctaaggcatg cacaattctt ttgagaggtg gaagcaagga tattttgaat gaaattgaaa gaaatctgga ggatgctttg catgttgtaa agaatatctt tattgatcct cgtattgttc ctggaggtgg tgctgttgaa atgagcgttt ctcaacattt gatgcaaaaa tcaaaaagta tcaagggaat tgaacaactt ccataccaag ctgttgcttc ttcattagag gttattccta gaacgttggc agaaaactgt ggagctaata caattcgtct catcactgaa ttgagagcta aacacgccaa tgaacaaaat cttactttgg gaattgatgg ccaaaagggt gttattgctg acactgcaga attgaagatt tgggaacctt acgaagtgaa ggttcaaacc attaagaccg ctgttgaggc agcttgtttg attcttcgta ttgacgacgt tttgtcctca ttaaagccta aggaaaagaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat