SSGCID: Target Page

NafoA.18143.a

phosphoglucomutase

CENTER ID:	NafoA.18143.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.18143.a.B1.GE40558	Full length(NafoA.18143.a)		1	598

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0129720

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLKIRSQST QPIPGQKMGT SGLRVKVKLV ENTPNFLENF TQSVLNIILA KDSLPQAAAH EDSYLQMVIG GDGRYYNKTA IQTILRILYS NAAHLGIKIH ALIGQNGIMS TPAVSCLIRK RKAELGGLIL TASHNPGGPN NDWGIKYNAV NGGPAPEKIT DRIYEQTTSI HEYYTCDEFP QIDLSQVNAE NGKGFVDYVF SDEHFRVTVV DSVNDYFELL SSIFDMEKIR KLIGKQFGEF NLLFDAMHGV TGSYAKRILH EKLGSDLHNA LMNYTPSEDF GGGHPDPNLT YAEHLVKRVL KDEKSNIDFG AASDGDGDRN MILGKHFFVT PSDSVAIIAD YAQKCIPYFQ KKGIAGLARS MPTSTALDRV AKELGVSMYE VPTGWKYFGN LFDAGKLSIC GEESFGTGSD HIREKDGMWA VLSWLSILAY ENEQAFSQNK KVSVADIVKN HWKKYGRSYY TRYDYEEVSA DGANKMMSHM RDTYIVPFQN NQLSEEAKNL FQTLNIASCE EFSYKDPIDN SFTGKQGMIF KLVDQSRIVF RLSGTGSAGA TIRVYMEKYS SDHLDGNPLE EVKPLAHVAL TVSKLNEFTG RNEPTVIT

NT Sequence

ATGCCTCTCA AAATTCGTTC CCAATCCACT CAACCCATTC CGGGACAAAA GATGGGTACT TCCGGCCTCC GAGTGAAGGT TAAGCTTGTT GAAAACACAC CAAATTTCCT TGAGAATTTC ACTCAGTCGG TTCTCAATAT TATTTTGGCC AAGGATTCTC TTCCTCAGGC TGCTGCTCAC GAGGATTCAT ATTTACAAAT GGTCATTGGA GGAGATGGTC GTTACTACAA CAAGACAGCC ATTCAAACCA TTCTCAGAAT CTTGTACTCG AATGCTGCTC ATCTCGGAAT TAAAATTCAT GCGTTGATTG GGCAGAATGG AATCATGTCC ACTCCTGCTG TTTCGTGCCT CATCAGAAAG AGAAAAGCAG AATTGGGAGG ATTGATTTTA ACTGCTTCAC ACAATCCTGG AGGTCCTAAC AATGATTGGG GAATCAAGTA CAATGCTGTT AATGGAGGTC CAGCACCAGA GAAAATCACC GATCGAATTT ATGAACAAAC AACAAGTATT CATGAATATT ACACTTGTGA TGAATTCCCA CAGATTGACT TGTCTCAGGT GAACGCAGAG AATGGAAAGG GATTTGTGGA TTATGTATTT TCGGATGAAC ATTTCCGTGT CACTGTGGTC GATTCTGTGA ACGATTACTT TGAGTTGTTA AGCTCAATAT TTGACATGGA AAAAATTAGA AAGCTCATCG GAAAGCAATT TGGTGAATTT AATTTATTAT TTGATGCCAT GCATGGCGTG ACAGGATCTT ATGCAAAGAG AATTTTGCAT GAGAAATTGG GATCAGATTT ACACAATGCT CTCATGAATT ACACTCCCAG TGAAGATTTC GGAGGAGGCC ATCCAGATCC AAATCTTACC TATGCTGAAC ATTTGGTTAA GAGAGTACTA AAGGATGAGA AGAGTAACAT TGACTTTGGA GCTGCCTCCG ATGGAGACGG AGATCGTAAC ATGATCTTGG GAAAGCATTT CTTTGTGACA CCTTCTGATT CAGTGGCTAT CATTGCTGAT TATGCTCAGA AATGCATTCC TTACTTCCAA AAGAAGGGTA TTGCTGGATT GGCTCGATCC ATGCCAACAA GCACTGCTCT TGACCGAGTC GCAAAAGAAT TAGGTGTCTC CATGTATGAG GTCCCAACTG GATGGAAATA CTTTGGTAAT TTATTTGATG CAGGAAAATT GTCTATTTGT GGCGAAGAAT CATTTGGAAC TGGAAGTGAC CACATTAGAG AAAAGGATGG CATGTGGGCT GTATTGAGCT GGCTTTCAAT TTTAGCCTAT GAGAATGAAC AAGCTTTCTC TCAAAACAAG AAGGTTTCTG TGGCTGACAT TGTTAAAAAT CATTGGAAGA AGTACGGTAG AAGCTACTAT ACCAGATATG ATTATGAGGA AGTGAGTGCC GATGGAGCAA ACAAAATGAT GTCACACATG AGGGACACAT ACATTGTTCC ATTCCAAAAT AATCAACTTT CTGAGGAAGC CAAGAATTTG TTCCAAACTC TCAATATTGC TTCATGTGAA GAATTCTCTT ATAAGGATCC AATTGATAAC AGTTTTACAG GAAAGCAAGG AATGATTTTC AAATTGGTCG ATCAAAGTAG AATCGTTTTC AGATTATCTG GAACTGGAAG TGCAGGAGCT ACCATCAGAG TTTACATGGA AAAGTATAGC TCTGACCATT TAGATGGAAA TCCATTGGAG GAAGTGAAGC CACTTGCTCA TGTTGCTCTC ACGGTCAGCA AATTGAATGA GTTTACTGGA AGAAATGAAC CAACAGTTAT CACT

Details for NafoA.18143.a.B1.GE40558

HARVESTED ON:	9/1/2016
SEQUENCED ON:	9/8/2016
EXPECTED MW:	68kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	82
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHXP LKIRSQSTQP IPGQKMGTSG LRVKVKLVEN TPNFLENFTQ SVLNIILAKD SLPQAAAHED SYLQMVIGGD GRYYNKTAIQ TILRILYSNA AHLGIKIHAL IGQNGIMSTP AVSCLIRKRK AELGGLILTA SHNPGGPNND WGIKYNAVNG GPAPEKITDR IYEQTTSIHE YYTCDEFPQI DLSQVNAENG KGFVDYVFSD EHFRVTVVDS VNDYFELLSS IFDXXKIXKL IGKQFGXXXL LFDAMHXVTX XXXXXXXXEX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXKXXXXXXX DSVXXXXXYA QKCIXXXXXX GIAGLARXXP TSTALDRVAK ELGVSMYEVP TGWKYFGNLF DAGKLSICGE ESFGTGSDHI REKDGMWAVL SWLSILAYEN EQAFSQNKKV SVADIVKNHW KKYGRSYYTR YDYEEVSADG ANKMMSHMRD TYIVPFQNNQ LSEEAKNLFQ TLNIASCEEF SYKDPIDNSF TGKQGMIFKL VDQSRIVFRL SGTGSAGATI RVYMEKYSSD HLDGNPLEEV KPLAXXXLTV SKLNE

Validated NT Sequence

ataccatggc tcaccaccac caccaccatn ngcctctcaa aattcgttcc caatccactc aacccattcc gggacaaaag atgggtactt ccggcctccg agtgaaggtt aagcttgttg aaaacacacc aaatttcctt gagaatttca ctcagtcggt tctcaatatt attttggcca aggattctct tcctcaggct gctgctcacg aggattcata tttacaaatg gtcattggag gagatggtcg ttactacaac aagacagcca ttcaaaccat tctcagaatc ttgtactcga atgctgctca tctcggaatt aaaattcatg cgttgattgg gcagaatgga atcatgtcca ctcctgctgt ttcgtgcctc atcagaaaga gaaaagcaga attgggagga ttgattttaa ctgcttcaca caatcctgga ggtcctaaca atgattgggg aatcaagtac aatgctgtta atggaggtcc agcaccagag aaaatcaccg atcgaattta tgaacaaaca acaagtattc atgaatatta cacttgtgat gaattcccac agattgactt gtctcaggtg aacgcagaga atggaaaggg atttgtggat tatgtatttt cggatgaaca tttccgtgtc actgtggtcg attctgtgaa cgattacttt gagttgttaa gctcaatatt tgacannnna aaaattnnna agctcatcgg aaagcaattt ggtgnnnnnn nnttattatt tgatgccatg catgncgtga cagnannntn nnnnnnnnnn nnnnnnnntg agnaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atnctgnnnn nnnnnnnnnn nnnnnnnnnn nggannnnnn nnnnnnnann nannnnnnnn nnnnnnnnnn nnnnnnnnnn gannntnaca tnnnnntnng aaagcnnnnn nnnnnnnnnn nnnnngattc agtgnnnnnc nnnnnnnntt atgctcagaa atgcattnnn nnnnnnnana nnnnnggtat tgctggattg gctcgnnnnn tgccaacaag cactgctctt gaccgagtcg caaaagaatt aggtgtctcc atgtatgagg tcccaactgg atggaaatac tttggtaatt tatttgatgc aggaaaattg tctatttgtg gcgaagaatc atttggaact ggaagtgacc acattagaga aaaggatggc atgtgggctg tattgagctg gctttcaatt ttagcctatg agaatgaaca agctttctct caaaacaaga aggtttctgt ggctgacatt gttaaaaatc attggaagaa gtacggtaga agctactata ccagatatga ttatgaggaa gtgagtgccg atggagcaaa caaaatgatg tcacacatga gggacacata cattgttcca ttccaaaata atcaactttc tgaggaagcc aagaatttgt tccaaactct caatattgct tcatgtgaag aattctctta taaggatcca attgataaca gttttacagg aaagcaagga atgattttca aattggtcga tcaaagtaga atcgttttca gattatctgg aactggaagt gcaggagcta ccatcagagt ttacatggaa aagtatagct ctgaccattt agatggaaat ccattggagg aagtgaagcc acttgcncnn nnnnnnctca cggtcagcaa attgaatgag ttt

Expected Protein Sequence

MAHHHHHHMP LKIRSQSTQP IPGQKMGTSG LRVKVKLVEN TPNFLENFTQ SVLNIILAKD SLPQAAAHED SYLQMVIGGD GRYYNKTAIQ TILRILYSNA AHLGIKIHAL IGQNGIMSTP AVSCLIRKRK AELGGLILTA SHNPGGPNND WGIKYNAVNG GPAPEKITDR IYEQTTSIHE YYTCDEFPQI DLSQVNAENG KGFVDYVFSD EHFRVTVVDS VNDYFELLSS IFDMEKIRKL IGKQFGEFNL LFDAMHGVTG SYAKRILHEK LGSDLHNALM NYTPSEDFGG GHPDPNLTYA EHLVKRVLKD EKSNIDFGAA SDGDGDRNMI LGKHFFVTPS DSVAIIADYA QKCIPYFQKK GIAGLARSMP TSTALDRVAK ELGVSMYEVP TGWKYFGNLF DAGKLSICGE ESFGTGSDHI REKDGMWAVL SWLSILAYEN EQAFSQNKKV SVADIVKNHW KKYGRSYYTR YDYEEVSADG ANKMMSHMRD TYIVPFQNNQ LSEEAKNLFQ TLNIASCEEF SYKDPIDNSF TGKQGMIFKL VDQSRIVFRL SGTGSAGATI RVYMEKYSSD HLDGNPLEEV KPLAHVALTV SKLNEFTGRN EPTVIT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctc tcaaaattcg ttcccaatcc actcaaccca ttccgggaca aaagatgggt acttccggcc tccgagtgaa ggttaagctt gttgaaaaca caccaaattt ccttgagaat ttcactcagt cggttctcaa tattattttg gccaaggatt ctcttcctca ggctgctgct cacgaggatt catatttaca aatggtcatt ggaggagatg gtcgttacta caacaagaca gccattcaaa ccattctcag aatcttgtac tcgaatgctg ctcatctcgg aattaaaatt catgcgttga ttgggcagaa tggaatcatg tccactcctg ctgtttcgtg cctcatcaga aagagaaaag cagaattggg aggattgatt ttaactgctt cacacaatcc tggaggtcct aacaatgatt ggggaatcaa gtacaatgct gttaatggag gtccagcacc agagaaaatc accgatcgaa tttatgaaca aacaacaagt attcatgaat attacacttg tgatgaattc ccacagattg acttgtctca ggtgaacgca gagaatggaa agggatttgt ggattatgta ttttcggatg aacatttccg tgtcactgtg gtcgattctg tgaacgatta ctttgagttg ttaagctcaa tatttgacat ggaaaaaatt agaaagctca tcggaaagca atttggtgaa tttaatttat tatttgatgc catgcatggc gtgacaggat cttatgcaaa gagaattttg catgagaaat tgggatcaga tttacacaat gctctcatga attacactcc cagtgaagat ttcggaggag gccatccaga tccaaatctt acctatgctg aacatttggt taagagagta ctaaaggatg agaagagtaa cattgacttt ggagctgcct ccgatggaga cggagatcgt aacatgatct tgggaaagca tttctttgtg acaccttctg attcagtggc tatcattgct gattatgctc agaaatgcat tccttacttc caaaagaagg gtattgctgg attggctcga tccatgccaa caagcactgc tcttgaccga gtcgcaaaag aattaggtgt ctccatgtat gaggtcccaa ctggatggaa atactttggt aatttatttg atgcaggaaa attgtctatt tgtggcgaag aatcatttgg aactggaagt gaccacatta gagaaaagga tggcatgtgg gctgtattga gctggctttc aattttagcc tatgagaatg aacaagcttt ctctcaaaac aagaaggttt ctgtggctga cattgttaaa aatcattgga agaagtacgg tagaagctac tataccagat atgattatga ggaagtgagt gccgatggag caaacaaaat gatgtcacac atgagggaca catacattgt tccattccaa aataatcaac tttctgagga agccaagaat ttgttccaaa ctctcaatat tgcttcatgt gaagaattct cttataagga tccaattgat aacagtttta caggaaagca aggaatgatt ttcaaattgg tcgatcaaag tagaatcgtt ttcagattat ctggaactgg aagtgcagga gctaccatca gagtttacat ggaaaagtat agctctgacc atttagatgg aaatccattg gaggaagtga agccacttgc tcatgttgct ctcacggtca gcaaattgaa tgagtttact ggaagaaatg aaccaacagt tatcacttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at