NafoA.18123.a

propionyl-carboxylase alpha mitochondrial

CENTER ID: NafoA.18123.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.18123.a.B1.GE40557 Full length(NafoA.18123.a) 1 795
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0005880

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIVISVPSS FITTTNIQAV NQQCLCPHRT VRRCRPREKM KNTLAKIVRC NKQLASKASS SYSVSSSRQG AFVLTTTSSS VECTNAQSLK LMRTPSSYSY KQFSTSVPKN FETTNNLKAP SQESSGEQKE HYFNKILIAN RGEIACRIIK TCRKLGIKTV AVYSDADANN KHVRMADESV YIGGSSPADS YLRADRIIAA MKKTGAEAVH PGFGFLSENA SFAKAIEESG LVFIGPPSSA ISAMGDKIES KIIAEKAKVN IIPGYQGEVH SADESVSIAE KIGYPVMIKA SAGGGGKGMR IAYNPEELRE GFRLSQEEAI RNFGSDKMLI ERFVENPRHI EIQIICDQHG NYLYLPEREC SIQRRNQKVI EEAPSSVIDE QTRKAMGEQA VALAKEVGYY SAGTVEMLID QNKNFYFLEM NTRLQVEHPI TEFITGIDLV EQMIRVAAKK PLILKQKDIK ITGHATECRV YAEDPMNNFA PSVGTLHTYI EPKDDEGRIR VDSGILEGSE ISVFYDPMIS KLITYGKDRI DSINRMVEAL DIYVIQGVRH NISLLRDILV TEEYRKGNIT TKFIPKHYPE GFKGIKLTPD QKSSLIASVG LMEYISSRRD FHISGQLPRA QAPKTLELVI NVDKEAYPVA VTQEKGKYIV ELKNQLYVAK TNWVYGTPIF RAVLNDTNSV IVQVGERKGT SQKVSFMGTE FKFEIRTPKE QELHKHMPVI IPPDLSKMLI APMPGAIVSV AVKPGQKVFP NQELVVMEAM KMQNVLRAAG EGVVKSVNVK PGDIVGNEAI LVEFE
NT Sequence
ATGACAATTG TAATCTCCGT TCCATCGTCA TTCATCACAA CAACAAACAT CCAGGCAGTC AATCAACAAT GCCTTTGTCC TCACCGCACC GTGCGCAGAT GTCGACCCCG TGAAAAAATG AAGAACACAC TCGCCAAAAT CGTTCGCTGC AACAAGCAAC TCGCATCAAA AGCATCATCA TCATACTCGG TGTCCTCATC AAGACAAGGA GCTTTTGTCT TGACAACCAC CAGCTCTAGT GTCGAATGTA CAAACGCTCA AAGTTTGAAG CTTATGAGAA CACCATCATC ATACTCCTAC AAGCAATTTT CAACAAGCGT CCCAAAGAAT TTTGAAACAA CAAACAATCT CAAAGCTCCA TCTCAAGAAT CATCAGGAGA GCAAAAGGAA CACTACTTCA ACAAAATATT GATCGCAAAC AGAGGTGAAA TCGCTTGTCG TATCATTAAA ACCTGTAGAA AGCTTGGTAT CAAAACAGTC GCTGTTTACA GTGATGCCGA TGCAAACAAC AAACACGTAA GAATGGCAGA TGAGTCTGTC TATATTGGTG GTTCATCTCC AGCTGATTCC TATCTCAGAG CTGACAGAAT TATTGCTGCC ATGAAAAAGA CTGGTGCCGA AGCGGTTCAT CCTGGTTTCG GTTTCCTCTC TGAGAATGCC TCTTTCGCAA AGGCTATTGA AGAATCTGGT CTAGTTTTTA TTGGTCCTCC TTCTTCAGCC ATCTCTGCTA TGGGTGATAA GATTGAAAGT AAAATTATTG CTGAAAAGGC CAAGGTCAAT ATTATTCCTG GTTATCAAGG AGAAGTACAC AGTGCTGATG AATCTGTTTC CATTGCTGAG AAAATTGGAT ATCCAGTCAT GATCAAGGCT TCAGCAGGTG GTGGTGGTAA GGGTATGAGA ATTGCTTATA ATCCAGAAGA GCTCAGAGAA GGTTTCCGAT TGAGTCAAGA GGAGGCCATC AGAAACTTTG GTAGTGATAA GATGTTGATT GAAAGATTTG TGGAAAATCC AAGACACATT GAAATTCAAA TTATTTGTGA CCAACATGGA AATTATCTTT ATTTACCTGA AAGAGAATGT TCCATCCAAA GAAGAAATCA AAAAGTTATT GAGGAGGCTC CATCTTCTGT CATTGATGAA CAAACTAGAA AGGCTATGGG TGAGCAAGCT GTTGCTTTGG CTAAGGAAGT CGGATATTAT TCAGCTGGTA CTGTAGAAAT GTTGATTGAT CAAAACAAGA ACTTCTACTT CTTGGAAATG AATACACGTT TGCAAGTTGA GCATCCAATT ACAGAATTTA TTACTGGAAT TGACTTGGTC GAGCAAATGA TTCGTGTTGC TGCAAAGAAA CCATTGATTT TGAAACAAAA GGATATTAAG ATTACTGGCC ATGCTACAGA ATGTAGAGTT TATGCCGAAG ATCCAATGAA TAACTTTGCT CCATCTGTTG GTACATTGCA CACTTATATT GAACCAAAGG ATGACGAGGG AAGAATTAGA GTTGATTCTG GTATTTTGGA AGGATCTGAG ATTTCTGTCT TCTACGATCC AATGATTTCC AAGCTCATTA CTTACGGAAA GGACAGAATT GATTCTATTA ACAGAATGGT GGAAGCACTA GACATTTATG TTATTCAAGG AGTTCGTCAC AATATTTCCT TGCTCCGTGA TATTTTGGTG ACTGAAGAAT ATAGGAAGGG TAATATTACA ACTAAGTTCA TTCCAAAGCA CTATCCTGAA GGATTCAAGG GCATCAAACT CACTCCTGAT CAAAAGAGTT CTCTCATTGC TTCAGTTGGT TTAATGGAAT ATATTTCATC AAGAAGAGAT TTCCATATTT CTGGACAATT ACCAAGAGCT CAAGCACCAA AGACACTTGA ACTTGTTATT AATGTCGACA AGGAGGCTTA TCCTGTTGCT GTCACACAAG AGAAGGGCAA ATATATCGTT GAACTCAAGA ATCAACTTTA TGTTGCCAAA ACAAATTGGG TTTATGGTAC TCCAATCTTC CGTGCTGTTC TTAATGACAC CAATTCAGTC ATTGTTCAAG TTGGCGAAAG AAAGGGTACT TCACAAAAGG TCAGCTTTAT GGGTACTGAA TTCAAATTTG AAATTAGAAC ACCAAAAGAG CAAGAATTGC ACAAACACAT GCCTGTCATT ATTCCACCAG ATCTTTCCAA GATGTTAATT GCTCCAATGC CTGGTGCTAT TGTTTCAGTG GCTGTCAAAC CAGGCCAAAA GGTCTTTCCA AATCAAGAAT TGGTTGTCAT GGAAGCAATG AAGATGCAAA ACGTGCTCAG AGCAGCAGGT GAAGGTGTTG TCAAGTCAGT GAATGTCAAA CCAGGAGACA TTGTTGGTAA TGAAGCTATT CTTGTTGAAT TTGAG
Details for NafoA.18123.a.B1.GE40557
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 89kDa
OBSERVED MW: 89kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT IVISVPSSFI TTTNIQAVNQ QCLCPHRTVR RCRPREKMKN TLAKIVRCNK QLASKASSSY SVSSSRQGAF VLTTTSSSVE CTNAQSLKLM RTPSSYSYKQ FSTSVPKNFE TTNNLKAPSQ ESSGEQKEHY FNKILIANRG EIACRIIKTC RKLGIKTVAV YSDADANNKH VRMADESVYI GGSSPADSYL RADRIIAAMK KTGAEAVHPG FGFLSENASF AKAIEESGLV FIGPPSSAIS AMGDKIESKI IAEKAKVNII PGYQGEVHSA DESVSIAEKI GYPVMIKASA GGGGKGMRIA YNPEELREGF RLSQEEAIRN FGSDKMLIER FVENPRHIEI QIICDQHGNY LYLPERECSI QRRNQKVIEE APSSVIDEQT RKAMGEQAVA LAKEVGYYSA GTVEMLIDQN KNFYFLEMNT RLQVEHPITE FITGIDLVEQ MIRVAAKKPL ILKQKDIKIT GHATECRVYA EDPMNNFAPS VGTLHTYIEP KDDEGRIRVD SGILEGSEIS VFYDPMISKL ITYGKDRIDS INRMVEALDI YVIQGVRHNI SLLRDILVTE EYRKGNITTK FIPKHYPEGF KGIKLTPDQK SSLIASVGLM EYISSRRDFH ISGQLPRAQA PKTLELVINV DKEAYPVAVT QEKGKYIVEL KNQLYVAKTN WVYGTPIFRA VLNDTNSVIV QVGERKGTSQ KVSFMGTEFK FEIRTPKEQE LHKHMPVIIP PDLSKMLIAP MPGAIVSVAV KPGQKVFPNQ ELVVMEAMKM QNVLRAAGEG VVKSVNVKPG DIVGNEAILV EFE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa ttgtaatctc cgttccatcg tcattcatca caacaacaaa catccaggca gtcaatcaac aatgcctttg tcctcaccgc accgtgcgca gatgtcgacc ccgtgaaaaa atgaagaaca cactcgccaa aatcgttcgc tgcaacaagc aactcgcatc aaaagcatca tcatcatact cggtgtcctc atcaagacaa ggagcttttg tcttgacaac caccagctct agtgtcgaat gtacaaacgc tcaaagtttg aagcttatga gaacaccatc atcatactcc tacaagcaat tttcaacaag cgtcccaaag aattttgaaa caacaaacaa tctcaaagct ccatctcaag aatcatcagg agagcaaaag gaacactact tcaacaaaat attgatcgca aacagaggtg aaatcgcttg tcgtatcatt aaaacctgta gaaagcttgg tatcaaaaca gtcgctgttt acagtgatgc cgatgcaaac aacaaacacg taagaatggc agatgagtct gtctatattg gtggttcatc tccagctgat tcctatctca gagctgacag aattattgct gccatgaaaa agactggtgc cgaagcggtt catcctggtt tcggtttcct ctctgagaat gcctctttcg caaaggctat tgaagaatct ggtctagttt ttattggtcc tccttcttca gccatctctg ctatgggtga taagattgaa agtaaaatta ttgctgaaaa ggccaaggtc aatattattc ctggttatca aggagaagta cacagtgctg atgaatctgt ttccattgct gagaaaattg gatatccagt catgatcaag gcttcagcag gtggtggtgg taagggtatg agaattgctt ataatccaga agagctcaga gaaggtttcc gattgagtca agaggaggcc atcagaaact ttggtagtga taagatgttg attgaaagat ttgtggaaaa tccaagacac attgaaattc aaattatttg tgaccaacat ggaaattatc tttatttacc tgaaagagaa tgttccatcc aaagaagaaa tcaaaaagtt attgaggagg ctccatcttc tgtcattgat gaacaaacta gaaaggctat gggtgagcaa gctgttgctt tggctaagga agtcggatat tattcagctg gtactgtaga aatgttgatt gatcaaaaca agaacttcta cttcttggaa atgaatacac gtttgcaagt tgagcatcca attacagaat ttattactgg aattgacttg gtcgagcaaa tgattcgtgt tgctgcaaag aaaccattga ttttgaaaca aaaggatatt aagattactg gccatgctac agaatgtaga gtttatgccg aagatccaat gaataacttt gctccatctg ttggtacatt gcacacttat attgaaccaa aggatgacga gggaagaatt agagttgatt ctggtatttt ggaaggatct gagatttctg tcttctacga tccaatgatt tccaagctca ttacttacgg aaaggacaga attgattcta ttaacagaat ggtggaagca ctagacattt atgttattca aggagttcgt cacaatattt ccttgctccg tgatattttg gtgactgaag aatataggaa gggtaatatt acaactaagt tcattccaaa gcactatcct gaaggattca agggcatcaa actcactcct gatcaaaaga gttctctcat tgcttcagtt ggtttaatgg aatatatttc atcaagaaga gatttccata tttctggaca attaccaaga gctcaagcac caaagacact tgaacttgtt attaatgtcg acaaggaggc ttatcctgtt gctgtcacac aagagaaggg caaatatatc gttgaactca agaatcaact ttatgttgcc aaaacaaatt gggtttatgg tactccaatc ttccgtgctg ttcttaatga caccaattca gtcattgttc aagttggcga aagaaagggt acttcacaaa aggtcagctt tatgggtact gaattcaaat ttgaaattag aacaccaaaa gagcaagaat tgcacaaaca catgcctgtc attattccac cagatctttc caagatgtta attgctccaa tgcctggtgc tattgtttca gtggctgtca aaccaggcca aaaggtcttt ccaaatcaag aattggttgt catggaagca atgaagatgc aaaacgtgct cagagcagca ggtgaaggtg ttgtcaagtc agtgaatgtc aaaccaggag acattgttgg taatgaagct attcttgttg aatttgagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat