NafoA.17190.a

cell division control protein 48 homolog e-like,transitional endoplasmic reticulum atpase ter94-like

CENTER ID:	NafoA.17190.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.17190.a.B1.GE40550	Full length(NafoA.17190.a)		1	822

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.17190.a.B1.PS38293	Full length(NafoA.17190.a)		1	822

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0107870

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDEQQEPTT PSSKRALERK KQHEELLAKV KSQKEKNRLV VDEATQDDNS IVGVHPDTME ALELFRGDTV RIVGKKHKDT IAIVLSDEEC EKGKIKMNKV MRKNLRVRLG DVIILKQCPD VKYGKRIHVL PIDDTVEGLT GDLFDIFLKP YFLEAYRPVR KGDLFNVRGG MRSVEFKVVE TDPDEYCIVA PDTVIHCEGD PIKREDEEAR LNEVGYDDIG GVRKQLAQIR EMVELPLRHP QLFKSIGIKP PRGILLYGPP GSGKTLIARA VANETGAFFF LINGPEIMSK LAGESEANLR KAFEEAEKNA PSIIFIDEID SIAPKREKAN GEVERRIVSQ LLTLMDGLNT RSNVIVMAAT NRPNSIDEAL RRFGRFDREI DIGVPDEIGR LEVLRIHTKN MKLDDDVDLE AVAKETHGYV GADLAQLCTE AAMNCIREKM DLIDLEEDTI DAAVLDSMGV TMDHFRAALT TQSPSSLRET VVEVPNVTWA DIGGLEKVKQ ELQELVQYPI EYPDMFAKFG QEASKGVLFY GPPGCGKTLL AKAIANECQA NFISVKGPEL LTMWFGESEA NVRNIFNKAR AAAPCVLFFD ELDSIAKARG GSLGDAGGAS DRVINQILTE MDGMGKKKNV FIIGATNRPD IIDPAVMRPG RLDQLIYIPL PDEGSRLSIL KASTRKSPLA SDVNLHAIAM ATKGFSGADL TEICQRAAKL AIRESIQREV EYRRQKEEER KSKQMEEEDE DEELEENMDF VPYITRAHFE EAMRFARKSV PEHEIRKYEM FSTSLQQAAG DVRNFKFSDY DTAGTTSTTT TTEEVMDTDL YQ

NT Sequence

ATGTCTGACG AACAACAAGA ACCAACAACA CCATCCTCCA AGAGAGCACT CGAGAGAAAG AAGCAACATG AAGAATTGTT GGCCAAGGTA AAATCTCAAA AGGAAAAGAA TCGATTGGTC GTTGATGAAG CCACTCAAGA TGATAACAGC ATTGTTGGTG TCCATCCAGA TACAATGGAA GCCCTAGAAT TGTTTAGAGG TGATACCGTC CGTATTGTTG GTAAGAAACA CAAGGATACC ATTGCTATTG TCTTGTCCGA TGAAGAATGT GAGAAGGGTA AAATCAAGAT GAACAAGGTG ATGAGAAAGA ACTTGCGTGT TCGTCTTGGT GATGTAATTA TTCTCAAACA ATGTCCCGAT GTAAAGTATG GTAAGAGAAT TCACGTTCTC CCAATTGATG ATACCGTTGA AGGCTTAACT GGTGATCTCT TTGACATCTT CTTGAAGCCA TACTTCCTTG AAGCCTACAG ACCAGTCAGA AAGGGTGATT TGTTCAATGT TCGCGGAGGT ATGAGATCGG TGGAATTCAA GGTCGTTGAG ACAGATCCAG ACGAATATTG TATTGTTGCT CCCGATACTG TCATTCACTG CGAAGGTGAT CCAATTAAGA GAGAAGATGA AGAAGCAAGA TTGAACGAAG TCGGCTATGA CGACATTGGT GGTGTAAGAA AGCAATTGGC TCAAATCAGA GAAATGGTTG AATTACCATT GAGACACCCA CAACTCTTCA AATCTATTGG TATCAAACCT CCTCGAGGTA TTCTCTTGTA CGGTCCTCCA GGTTCGGGTA AGACTCTGAT CGCTCGAGCA GTAGCCAATG AAACTGGTGC ATTCTTCTTC TTGATCAATG GTCCAGAAAT TATGTCCAAG TTAGCTGGTG AGTCTGAAGC AAACTTGAGA AAGGCCTTCG AAGAAGCAGA AAAGAATGCT CCTTCGATTA TCTTTATCGA CGAAATAGAC TCGATTGCTC CAAAGAGAGA AAAGGCCAAC GGTGAAGTTG AAAGAAGAAT TGTTTCTCAA TTACTTACTC TCATGGACGG TTTGAATACA AGATCAAACG TGATTGTCAT GGCAGCAACA AATAGACCAA ATTCAATTGA TGAAGCTCTT CGTAGATTCG GTCGTTTCGA CAGAGAAATT GATATTGGTG TGCCTGATGA AATTGGAAGA TTGGAAGTTT TAAGAATTCA CACAAAAAAT ATGAAATTGG ATGACGATGT TGATTTGGAG GCTGTTGCCA AGGAAACTCA CGGTTATGTC GGTGCCGATC TTGCTCAATT GTGTACTGAA GCTGCCATGA ACTGTATCAG AGAAAAGATG GACTTGATTG ACTTGGAAGA AGATACCATT GATGCTGCAG TTTTGGACTC TATGGGAGTC ACAATGGATC ACTTTAGAGC TGCTCTCACT ACTCAATCAC CATCATCCCT CAGAGAAACT GTTGTTGAAG TTCCAAATGT CACATGGGCA GATATCGGTG GTTTGGAGAA GGTCAAACAA GAATTGCAAG AATTGGTTCA ATATCCAATC GAATATCCAG ACATGTTTGC CAAATTCGGT CAAGAAGCTT CAAAGGGTGT CCTCTTCTAT GGTCCTCCTG GATGTGGTAA GACACTTCTC GCTAAGGCCA TCGCAAACGA ATGCCAAGCA AACTTTATTT CTGTTAAGGG TCCAGAATTG TTGACCATGT GGTTCGGTGA ATCTGAAGCT AACGTTAGAA ACATTTTCAA CAAGGCTAGA GCTGCTGCTC CTTGTGTTTT GTTCTTTGAC GAATTGGACT CAATTGCCAA GGCAAGAGGT GGAAGTCTCG GTGATGCTGG TGGTGCTTCT GACAGAGTTA TTAACCAAAT TCTTACAGAA ATGGACGGTA TGGGTAAGAA GAAGAACGTT TTCATTATTG GTGCTACCAA TAGACCAGAT ATTATTGATC CTGCAGTCAT GAGACCAGGT CGTTTGGATC AATTGATTTA CATTCCATTG CCAGATGAAG GTTCAAGACT CTCAATTTTG AAGGCATCCA CCCGAAAGTC GCCACTTGCT TCTGATGTCA ACTTGCACGC AATTGCTATG GCCACTAAGG GATTCTCTGG TGCTGACTTG ACTGAAATTT GTCAACGTGC TGCCAAGTTG GCCATTCGTG AGTCCATTCA GAGAGAAGTC GAATATAGAA GACAAAAGGA AGAGGAAAGA AAATCAAAGC AAATGGAAGA GGAAGATGAA GACGAAGAAT TGGAGGAAAA TATGGACTTT GTCCCTTATA TTACCAGAGC TCACTTCGAA GAGGCAATGA GATTTGCTCG TAAATCAGTC CCAGAACATG AAATTAGAAA GTATGAAATG TTCTCGACTT CCTTGCAACA AGCTGCAGGT GATGTCAGAA ACTTCAAGTT CTCGGATTAT GATACTGCTG GTACAACCTC AACAACTACC ACAACTGAAG AGGTCATGGA CACTGATCTC TACCAA

Details for NafoA.17190.a.B1.GE40550

HARVESTED ON:	9/1/2016
SEQUENCED ON:	9/8/2016
EXPECTED MW:	93kDa
OBSERVED MW:	100kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS DEQQEPTTPS SKRALERKKQ HEELLAKVKS QKEKNRLVVD EATQDDNSIV GVHPDTMEAL ELFRGDTVRI VGKKHKDTIA IVLSDEECEK GKIKMNKVMR KNLRVRLGDV IILKQCPDVK YGKRIHVLPI DDTVEGLTGD LFDIFLKPYF LEAYRPVRKG DLFNVRGGMR SVEFKVVETD PDEYCIVAPD TVIHCEGDPI KREDEEARLN EVGYDDIGGV RKQLAQIREM VELPLRHPQL FKSIGIKPPR GILLYGPPGS GKTLIARAVA NETGAFFFLI NGPEIMSKLA GESEANLRKA FEEAEKNAPS IIFIDEIDSI APKREKANGE VERRIVSQLL TLMDGLNTRS NVIVMAATNR PNSIDEALRR FGRFDREIDI GVPDEIGRLE VLRIHTKNMK LDDDVDLEAV AKETHGYVGA DLAQLCTEAA MNCIREKMDL IDLEEDTIDA AVLDSMGVTM DHFRAALTTQ SPSSLRETVV EVPNVTWADI GGLEKVKQEL QELVQYPIEY PDMFAKFGQE ASKGVLFYGP PGCGKTLLAK AIANECQANF ISVKGPELLT MWFGESEANV RNIFNKARAA APCVLFFDEL DSIAKARGGS LGDAGGASDR VINQILTEMD GMGKKKNVFI IGATNRPDII DPAVMRPGRL DQLIYIPLPD EGSRLSILKA STRKSPLASD VNLHAIAMAT KGFSGADLTE ICQRAAKLAI RESIQREVEY RRQKEEERKS KQMEEEDEDE ELEENMDFVP YITRAHFEEA MRFARKSVPE HEIRKYEMFS TSLQQAAGDV RNFKFSDYDT AGTTSTTTTT EEVMDTDLYQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg acgaacaaca agaaccaaca acaccatcct ccaagagagc actcgagaga aagaagcaac atgaagaatt gttggccaag gtaaaatctc aaaaggaaaa gaatcgattg gtcgttgatg aagccactca agatgataac agcattgttg gtgtccatcc agatacaatg gaagccctag aattgtttag aggtgatacc gtccgtattg ttggtaagaa acacaaggat accattgcta ttgtcttgtc cgatgaagaa tgtgagaagg gtaaaatcaa gatgaacaag gtgatgagaa agaacttgcg tgttcgtctt ggtgatgtaa ttattctcaa acaatgtccc gatgtaaagt atggtaagag aattcacgtt ctcccaattg atgataccgt tgaaggctta actggtgatc tctttgacat cttcttgaag ccatacttcc ttgaagccta cagaccagtc agaaagggtg atttgttcaa tgttcgcgga ggtatgagat cggtggaatt caaggtcgtt gagacagatc cagacgaata ttgtattgtt gctcccgata ctgtcattca ctgcgaaggt gatccaatta agagagaaga tgaagaagca agattgaacg aagtcggcta tgacgacatt ggtggtgtaa gaaagcaatt ggctcaaatc agagaaatgg ttgaattacc attgagacac ccacaactct tcaaatctat tggtatcaaa cctcctcgag gtattctctt gtacggtcct ccaggttcgg gtaagactct gatcgctcga gcagtagcca atgaaactgg tgcattcttc ttcttgatca atggtccaga aattatgtcc aagttagctg gtgagtctga agcaaacttg agaaaggcct tcgaagaagc agaaaagaat gctccttcga ttatctttat cgacgaaata gactcgattg ctccaaagag agaaaaggcc aacggtgaag ttgaaagaag aattgtttct caattactta ctctcatgga cggtttgaat acaagatcaa acgtgattgt catggcagca acaaatagac caaattcaat tgatgaagct cttcgtagat tcggtcgttt cgacagagaa attgatattg gtgtgcctga tgaaattgga agattggaag ttttaagaat tcacacaaaa aatatgaaat tggatgacga tgttgatttg gaggctgttg ccaaggaaac tcacggttat gtcggtgccg atcttgctca attgtgtact gaagctgcca tgaactgtat cagagaaaag atggacttga ttgacttgga agaagatacc attgatgctg cagttttgga ctctatggga gtcacaatgg atcactttag agctgctctc actactcaat caccatcatc cctcagagaa actgttgttg aagttccaaa tgtcacatgg gcagatatcg gtggtttgga gaaggtcaaa caagaattgc aagaattggt tcaatatcca atcgaatatc cagacatgtt tgccaaattc ggtcaagaag cttcaaaggg tgtcctcttc tatggtcctc ctggatgtgg taagacactt ctcgctaagg ccatcgcaaa cgaatgccaa gcaaacttta tttctgttaa gggtccagaa ttgttgacca tgtggttcgg tgaatctgaa gctaacgtta gaaacatttt caacaaggct agagctgctg ctccttgtgt tttgttcttt gacgaattgg actcaattgc caaggcaaga ggtggaagtc tcggtgatgc tggtggtgct tctgacagag ttattaacca aattcttaca gaaatggacg gtatgggtaa gaagaagaac gttttcatta ttggtgctac caatagacca gatattattg atcctgcagt catgagacca ggtcgtttgg atcaattgat ttacattcca ttgccagatg aaggttcaag actctcaatt ttgaaggcat ccacccgaaa gtcgccactt gcttctgatg tcaacttgca cgcaattgct atggccacta agggattctc tggtgctgac ttgactgaaa tttgtcaacg tgctgccaag ttggccattc gtgagtccat tcagagagaa gtcgaatata gaagacaaaa ggaagaggaa agaaaatcaa agcaaatgga agaggaagat gaagacgaag aattggagga aaatatggac tttgtccctt atattaccag agctcacttc gaagaggcaa tgagatttgc tcgtaaatca gtcccagaac atgaaattag aaagtatgaa atgttctcga cttccttgca acaagctgca ggtgatgtca gaaacttcaa gttctcggat tatgatactg ctggtacaac ctcaacaact accacaactg aagaggtcat ggacactgat ctctaccaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for NafoA.17190.a.B1.PS38293

PURIFICATION DATe:	6/2/2017
CONCENTRATION:	30.5mg/ml
OBSERVED MW:	95kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG ACGAACAACA AGAACCAACA ACACCATCCT CCAAGAGAGC ACTCGAGAGA AAGAAGCAAC ATGAAGAATT GTTGGCCAAG GTAAAATCTC AAAAGGAAAA GAATCGATTG GTCGTTGATG AAGCCACTCA AGATGATAAC AGCATTGTTG GTGTCCATCC AGATACAATG GAAGCCCTAG AATTGTTTAG AGGTGATACC GTCCGTATTG TTGGTAAGAA ACACAAGGAT ACCATTGCTA TTGTCTTGTC CGATGAAGAA TGTGAGAAGG GTAAAATCAA GATGAACAAG GTGATGAGAA AGAACTTGCG TGTTCGTCTT GGTGATGTAA TTATTCTCAA ACAATGTCCC GATGTAAAGT ATGGTAAGAG AATTCACGTT CTCCCAATTG ATGATACCGT TGAAGGCTTA ACTGGTGATC TCTTTGACAT CTTCTTGAAG CCATACTTCC TTGAAGCCTA CAGACCAGTC AGAAAGGGTG ATTTGTTCAA TGTTCGCGGA GGTATGAGAT CGGTGGAATT CAAGGTCGTT GAGACAGATC CAGACGAATA TTGTATTGTT GCTCCCGATA CTGTCATTCA CTGCGAAGGT GATCCAATTA AGAGAGAAGA TGAAGAAGCA AGATTGAACG AAGTCGGCTA TGACGACATT GGTGGTGTAA GAAAGCAATT GGCTCAAATC AGAGAAATGG TTGAATTACC ATTGAGACAC CCACAACTCT TCAAATCTAT TGGTATCAAA CCTCCTCGAG GTATTCTCTT GTACGGTCCT CCAGGTTCGG GTAAGACTCT GATCGCTCGA GCAGTAGCCA ATGAAACTGG TGCATTCTTC TTCTTGATCA ATGGTCCAGA AATTATGTCC AAGTTAGCTG GTGAGTCTGA AGCAAACTTG AGAAAGGCCT TCGAAGAAGC AGAAAAGAAT GCTCCTTCGA TTATCTTTAT CGACGAAATA GACTCGATTG CTCCAAAGAG AGAAAAGGCC AACGGTGAAG TTGAAAGAAG AATTGTTTCT CAATTACTTA CTCTCATGGA CGGTTTGAAT ACAAGATCAA ACGTGATTGT CATGGCAGCA ACAAATAGAC CAAATTCAAT TGATGAAGCT CTTCGTAGAT TCGGTCGTTT CGACAGAGAA ATTGATATTG GTGTGCCTGA TGAAATTGGA AGATTGGAAG TTTTAAGAAT TCACACAAAA AATATGAAAT TGGATGACGA TGTTGATTTG GAGGCTGTTG CCAAGGAAAC TCACGGTTAT GTCGGTGCCG ATCTTGCTCA ATTGTGTACT GAAGCTGCCA TGAACTGTAT CAGAGAAAAG ATGGACTTGA TTGACTTGGA AGAAGATACC ATTGATGCTG CAGTTTTGGA CTCTATGGGA GTCACAATGG ATCACTTTAG AGCTGCTCTC ACTACTCAAT CACCATCATC CCTCAGAGAA ACTGTTGTTG AAGTTCCAAA TGTCACATGG GCAGATATCG GTGGTTTGGA GAAGGTCAAA CAAGAATTGC AAGAATTGGT TCAATATCCA ATCGAATATC CAGACATGTT TGCCAAATTC GGTCAAGAAG CTTCAAAGGG TGTCCTCTTC TATGGTCCTC CTGGATGTGG TAAGACACTT CTCGCTAAGG CCATCGCAAA CGAATGCCAA GCAAACTTTA TTTCTGTTAA GGGTCCAGAA TTGTTGACCA TGTGGTTCGG TGAATCTGAA GCTAACGTTA GAAACATTTT CAACAAGGCT AGAGCTGCTG CTCCTTGTGT TTTGTTCTTT GACGAATTGG ACTCAATTGC CAAGGCAAGA GGTGGAAGTC TCGGTGATGC TGGTGGTGCT TCTGACAGAG TTATTAACCA AATTCTTACA GAAATGGACG GTATGGGTAA GAAGAAGAAC GTTTTCATTA TTGGTGCTAC CAATAGACCA GATATTATTG ATCCTGCAGT CATGAGACCA GGTCGTTTGG ATCAATTGAT TTACATTCCA TTGCCAGATG AAGGTTCAAG ACTCTCAATT TTGAAGGCAT CCACCCGAAA GTCGCCACTT GCTTCTGATG TCAACTTGCA CGCAATTGCT ATGGCCACTA AGGGATTCTC TGGTGCTGAC TTGACTGAAA TTTGTCAACG TGCTGCCAAG TTGGCCATTC GTGAGTCCAT TCAGAGAGAA GTCGAATATA GAAGACAAAA GGAAGAGGAA AGAAAATCAA AGCAAATGGA AGAGGAAGAT GAAGACGAAG AATTGGAGGA AAATATGGAC TTTGTCCCTT ATATTACCAG AGCTCACTTC GAAGAGGCAA TGAGATTTGC TCGTAAATCA GTCCCAGAAC ATGAAATTAG AAAGTATGAA ATGTTCTCGA CTTCCTTGCA ACAAGCTGCA GGTGATGTCA GAAACTTCAA GTTCTCGGAT TATGATACTG CTGGTACAAC CTCAACAACT ACCACAACTG AAGAGGTCAT GGACACTGAT CTCTACCAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT