NafoA.17129.a

heat shock protein 81-1-like isoform x2,heat shock protein 83-like,heat shock protein 90-2 (hsp90)

CENTER ID: NafoA.17129.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.17129.a.B1.GE40549 Full length(NafoA.17129.a) 1 699
NafoA.17129.a.B2.GE40626 ATPase domain 5 210
NafoA.17129.a.B3.GE40627 middle and C-terminal domain 264 663
NafoA.17129.a.B4.GE40628 ordered HSP90 domain 5 663
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.17129.a.B2.PW37999 ATPase domain 5 210
NafoA.17129.a.B3.PW38000 middle and C-terminal domain 264 663

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0082340

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTETYKFQA EINQLMSLII NTFYSNKEIF LRELISNASD ALDKIRHLSL TDPSVLDTEK ELYIRIIPDK TNKTLTIQDS GVGMTKADLI NCLGTIARSG TKQFMEMLQA GADISLIGQF GVGFYSAYLV ADRVVVVTKH NDDEQYIWES AAGGSFTITL DESGEKMTRG TKIILHMKED QLEYLEERRL KDLVKKHSEF IGYPISLYVE KTSEKEVEEE EEPEEKEDKE EPKIEEVDEE KEKKKKKVKE VTHEWELLNK TKPLWTRDPK SITKEEYASF YKALTNDWEE HLAVKHFSVE GQLEFKAILF VPKRAPFDLF EPKKKLNNIK LYVRRVFIMD NCEELIPEYL NFIKGIVDSE DLPLNISREH LQQNKIMKVI RKNLVKKCIE MFSEIAENKD DFKAFYESFG KNLKLGIHED SQNRQKLAEL LRFHSSTSGE EWTSLKEYVE RMKEGQEDIY YITGESKKAV SKSPFIEKCT KKGFEVLYMT DPIDEYMVQQ LKEFDGKKLV CVTKEGLKLP ETEEEKKKKE ELKASFESLC KLMKDILGDK VEKVVVSDRL GDSPCCLVTG EYGWSANMER IMKAQALKDN SMAAYMVSKK TMEINPENSI VNELRKKAEA NKADKTLRDL VWLLFDTSLL TSGFSLEEPS IYASRIHRMI KLGLSIDEDE SSTASSSSEE KKEQVAEEPA AESLMEDVD
NT Sequence
ATGTCCACAG AAACCTACAA ATTCCAAGCT GAAATCAATC AATTGATGAG TTTAATCATC AATACTTTTT ATAGCAATAA GGAAATCTTC TTGAGAGAGT TAATTTCCAA TGCTTCTGAT GCTTTGGATA AAATTCGCCA CTTGAGTTTG ACTGACCCTT CAGTCTTGGA CACTGAAAAA GAATTGTATA TTCGTATCAT TCCAGATAAG ACTAACAAGA CATTGACCAT TCAAGATTCA GGTGTTGGTA TGACAAAAGC TGATTTAATT AATTGTCTTG GTACGATCGC CAGATCCGGT ACAAAACAAT TCATGGAAAT GCTTCAAGCA GGAGCTGACA TTTCTTTGAT TGGTCAATTC GGTGTCGGTT TTTACAGTGC TTACTTGGTT GCAGATCGAG TGGTTGTAGT GACCAAGCAC AATGATGACG AACAATACAT TTGGGAATCT GCTGCTGGTG GTAGCTTTAC CATTACTTTG GATGAGAGTG GTGAAAAAAT GACTCGTGGT ACCAAGATTA TCTTGCACAT GAAGGAAGAT CAATTGGAAT ATTTGGAGGA ACGTCGATTG AAGGATCTTG TCAAGAAGCA CTCTGAATTT ATTGGTTATC CAATCTCCTT GTACGTCGAA AAGACCAGCG AAAAGGAGGT GGAAGAAGAA GAAGAACCTG AAGAAAAGGA GGACAAGGAA GAACCAAAGA TTGAAGAAGT CGACGAAGAG AAGGAAAAGA AGAAGAAGAA GGTGAAGGAG GTGACCCATG AATGGGAATT GCTCAACAAG ACCAAGCCTC TCTGGACTCG TGACCCAAAG AGCATCACCA AGGAAGAATA TGCAAGCTTT TACAAGGCTT TGACCAACGA TTGGGAGGAA CATTTGGCTG TCAAACACTT CTCTGTTGAA GGTCAACTCG AATTCAAGGC CATCTTGTTC GTTCCAAAGC GTGCTCCATT TGATTTGTTC GAACCAAAGA AGAAGTTGAA CAATATCAAA CTCTACGTGA GAAGAGTCTT TATTATGGAC AATTGTGAAG AATTAATTCC AGAATACTTG AACTTTATTA AGGGTATTGT TGACAGTGAG GACTTGCCAT TGAACATTTC TCGTGAGCAC TTGCAACAAA ACAAGATTAT GAAGGTGATT AGAAAGAACT TGGTCAAGAA GTGCATTGAA ATGTTCTCTG AAATTGCTGA AAACAAGGAT GATTTCAAGG CCTTTTATGA ATCCTTTGGT AAGAACTTGA AGTTGGGAAT TCACGAAGAC TCTCAAAACA GACAAAAGTT GGCTGAATTG CTCAGATTCC ACTCATCCAC TTCGGGCGAG GAATGGACAA GCCTTAAGGA ATATGTGGAA AGAATGAAGG AAGGTCAAGA AGACATTTAC TACATTACTG GTGAAAGCAA GAAGGCTGTC TCCAAATCTC CATTCATTGA AAAGTGTACC AAGAAGGGCT TTGAAGTCTT GTACATGACT GATCCTATTG ATGAATACAT GGTTCAACAA TTGAAGGAAT TTGATGGTAA AAAGTTGGTC TGTGTCACCA AGGAAGGTTT GAAGTTGCCA GAAACTGAAG AGGAAAAGAA GAAGAAGGAA GAATTGAAGG CCTCTTTCGA ATCCTTGTGC AAGTTGATGA AGGACATTTT GGGTGACAAG GTCGAGAAAG TGGTTGTTTC CGATAGACTT GGTGACTCTC CATGTTGCTT GGTCACTGGT GAATATGGAT GGAGTGCAAA CATGGAAAGA ATCATGAAGG CTCAAGCTTT GAAGGATAAC TCAATGGCTG CTTACATGGT TTCTAAAAAG ACTATGGAAA TTAACCCAGA AAACTCGATC GTCAATGAAT TGAGAAAGAA GGCTGAGGCT AACAAGGCTG ACAAGACTTT GAGAGATTTG GTCTGGCTCT TGTTTGACAC TTCCTTGTTG ACTTCTGGAT TCTCATTGGA GGAACCAAGC ATTTATGCTT CTCGTATTCA CAGAATGATC AAACTTGGTT TGAGCATTGA TGAAGACGAA TCTTCCACTG CTTCATCATC CTCTGAGGAG AAGAAGGAAC AAGTTGCAGA AGAACCTGCT GCTGAATCCT TGATGGAAGA TGTTGAT
Details for NafoA.17129.a.B1.GE40549
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 82kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS TETYKFQAEI NQLMSLIINT FYSNKEIFLR ELISNASDAL DKIRHLSLTD PSVLDTEKEL YIRIIPDKTN KTLTIQDSGV GMTKADLINC LGTIARSGTK QFMEMLQAGA DISLIGQFGV GFYSAYLVAD RVVVVTKHND DEQYIWESAA GGSFTITLDE SGEKMTRGTK IILHMKEDQL EYLEERRLKD LVKKHSEFIG YPISLYVEKT SEKEVEEEEE PEEKEDKEEP KIEEVDEEKE KKKKKVKEVT HEWELLNKTK PLWTRDPKSI TKEEYASFYK ALTNDWEEHL AVKHFSVEGQ LEFKAILFVP KRAPFDLFEP KKKLNNIKLY VRRVFIMDNC EELIPEYLNF IKGIVDSEDL PLNISREHLQ QNKIMKVIRK NLVKKCIEMF SEIAENKDDF KAFYESFGKN LKLGIHEDSQ NRQKLAELLR FHSSTSGEEW TSLKEYVERM KEGQEDIYYI TGESKKAVSK SPFIEKCTKK GFEVLYMTDP IDEYMVQQLK EFDGKKLVCV TKEGLKLPET EEEKKKKEEL KASFESLCKL MKDILGDKVE KVVVSDRLGD SPCCLVTGEY GWSANMERIM KAQALKDNSM AAYMVSKKTM EINPENSIVN ELRKKAEANK ADKTLRDLVW LLFDTSLLTS GFSLEEPSIY ASRIHRMIKL GLSIDEDESS TASSSSEEKK EQVAEEPAAE SLMEDVD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca cagaaaccta caaattccaa gctgaaatca atcaattgat gagtttaatc atcaatactt tttatagcaa taaggaaatc ttcttgagag agttaatttc caatgcttct gatgctttgg ataaaattcg ccacttgagt ttgactgacc cttcagtctt ggacactgaa aaagaattgt atattcgtat cattccagat aagactaaca agacattgac cattcaagat tcaggtgttg gtatgacaaa agctgattta attaattgtc ttggtacgat cgccagatcc ggtacaaaac aattcatgga aatgcttcaa gcaggagctg acatttcttt gattggtcaa ttcggtgtcg gtttttacag tgcttacttg gttgcagatc gagtggttgt agtgaccaag cacaatgatg acgaacaata catttgggaa tctgctgctg gtggtagctt taccattact ttggatgaga gtggtgaaaa aatgactcgt ggtaccaaga ttatcttgca catgaaggaa gatcaattgg aatatttgga ggaacgtcga ttgaaggatc ttgtcaagaa gcactctgaa tttattggtt atccaatctc cttgtacgtc gaaaagacca gcgaaaagga ggtggaagaa gaagaagaac ctgaagaaaa ggaggacaag gaagaaccaa agattgaaga agtcgacgaa gagaaggaaa agaagaagaa gaaggtgaag gaggtgaccc atgaatggga attgctcaac aagaccaagc ctctctggac tcgtgaccca aagagcatca ccaaggaaga atatgcaagc ttttacaagg ctttgaccaa cgattgggag gaacatttgg ctgtcaaaca cttctctgtt gaaggtcaac tcgaattcaa ggccatcttg ttcgttccaa agcgtgctcc atttgatttg ttcgaaccaa agaagaagtt gaacaatatc aaactctacg tgagaagagt ctttattatg gacaattgtg aagaattaat tccagaatac ttgaacttta ttaagggtat tgttgacagt gaggacttgc cattgaacat ttctcgtgag cacttgcaac aaaacaagat tatgaaggtg attagaaaga acttggtcaa gaagtgcatt gaaatgttct ctgaaattgc tgaaaacaag gatgatttca aggcctttta tgaatccttt ggtaagaact tgaagttggg aattcacgaa gactctcaaa acagacaaaa gttggctgaa ttgctcagat tccactcatc cacttcgggc gaggaatgga caagccttaa ggaatatgtg gaaagaatga aggaaggtca agaagacatt tactacatta ctggtgaaag caagaaggct gtctccaaat ctccattcat tgaaaagtgt accaagaagg gctttgaagt cttgtacatg actgatccta ttgatgaata catggttcaa caattgaagg aatttgatgg taaaaagttg gtctgtgtca ccaaggaagg tttgaagttg ccagaaactg aagaggaaaa gaagaagaag gaagaattga aggcctcttt cgaatccttg tgcaagttga tgaaggacat tttgggtgac aaggtcgaga aagtggttgt ttccgataga cttggtgact ctccatgttg cttggtcact ggtgaatatg gatggagtgc aaacatggaa agaatcatga aggctcaagc tttgaaggat aactcaatgg ctgcttacat ggtttctaaa aagactatgg aaattaaccc agaaaactcg atcgtcaatg aattgagaaa gaaggctgag gctaacaagg ctgacaagac tttgagagat ttggtctggc tcttgtttga cacttccttg ttgacttctg gattctcatt ggaggaacca agcatttatg cttctcgtat tcacagaatg atcaaacttg gtttgagcat tgatgaagac gaatcttcca ctgcttcatc atcctctgag gagaagaagg aacaagttgc agaagaacct gctgctgaat ccttgatgga agatgttgat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NafoA.17129.a.B2.GE40626
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT YKFQAEINQL MSLIINTFYS NKEIFLRELI SNASDALDKI RHLSLTDPSV LDTEKELYIR IIPDKTNKTL TIQDSGVGMT KADLINCLGT IARSGTKQFM EMLQAGADIS LIGQFGVGFY SAYLVADRVV VVTKHNDDEQ YIWESAAGGS FTITLDESGE KMTRGTKIIL HMKEDQLEYL EERRLKDLVK KHSEFIGYPI SLYVE
Validated NT Sequence
atcttactca cttattcgac gtacaaggag attggataac caataaattc agagtgcttc ttgacaagat ccttcaatcg acgttcctcc aaatattcca attgatcttc cttcatgtgc aagataatct tggtaccacg agtcattttt tcaccactct catccaaagt aatggtaaag ctaccaccag cagcagattc ccaaatgtat tgttcgtcat cattgtgctt ggtcactaca accactcgat ctgcaaccaa gtaagcactg taaaaaccga caccgaattg accaatcaaa gaaatgtcag ctcctgcttg aagcatttcc atgaattgtt ttgtaccgga tctggcgatc gtaccaagac aattaattaa atcagctttt gtcataccaa cacctgaatc ttgaatggtc aatgtcttgt tagtcttatc tggaatgata cgaatataca attctttttc agtgtccaag actgaagggt cagtcaaact caagtggcga attttatcca aagcatcaga agcattggaa attaactctc tcaagaagat ttccttattg ctataaaaag tattgatgat taaactcatc aattgattga tttcagcttg gaatttgtag gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHTY KFQAEINQLM SLIINTFYSN KEIFLRELIS NASDALDKIR HLSLTDPSVL DTEKELYIRI IPDKTNKTLT IQDSGVGMTK ADLINCLGTI ARSGTKQFME MLQAGADISL IGQFGVGFYS AYLVADRVVV VTKHNDDEQY IWESAAGGSF TITLDESGEK MTRGTKIILH MKEDQLEYLE ERRLKDLVKK HSEFIGYPIS LYVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacctaca aattccaagc tgaaatcaat caattgatga gtttaatcat caatactttt tatagcaata aggaaatctt cttgagagag ttaatttcca atgcttctga tgctttggat aaaattcgcc acttgagttt gactgaccct tcagtcttgg acactgaaaa agaattgtat attcgtatca ttccagataa gactaacaag acattgacca ttcaagattc aggtgttggt atgacaaaag ctgatttaat taattgtctt ggtacgatcg ccagatccgg tacaaaacaa ttcatggaaa tgcttcaagc aggagctgac atttctttga ttggtcaatt cggtgtcggt ttttacagtg cttacttggt tgcagatcga gtggttgtag tgaccaagca caatgatgac gaacaataca tttgggaatc tgctgctggt ggtagcttta ccattacttt ggatgagagt ggtgaaaaaa tgactcgtgg taccaagatt atcttgcaca tgaaggaaga tcaattggaa tatttggagg aacgtcgatt gaaggatctt gtcaagaagc actctgaatt tattggttat ccaatctcct tgtacgtcga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NafoA.17129.a.B3.GE40627
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLW TRDPKSITKE EYASFYKALT NDWEEHLAVK HFSVEGQLEF KAILFVPKRA PFDLFEPKKK LNNIKLYVRR VFIMDNCEEL IPEYLNFIKG IVDSEDLPLN ISREHLQQNK IMKVIRKNLV KKCIEMFSEI AENKDDFKAF YESFGKNLKL GIHEDSQNRQ KLAELLRFHS STSGEEWTSL KEYVERMKEG QEDIYYITGE SKKAVSKSPF IEKCTKKGFE VLYMTDPIDE YMVQQLKEFD GKKLVCVTKE GLKLPETEEE KKKKEELKAS FESLCKLMKD ILGDKVEKVV VSDRLGDSPC CLVTGEYGWS ANMERIMKAQ ALKDNSMAAY MVSKKTMEIN PENSIVNELR KKAEANKADK TLRDLVWLLF DTSLLTSGFS LEEPSIYASR IHRMIKLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctctgga ctcgtgaccc aaagagcatc accaaggaag aatatgcaag cttttacaag gctttgacca acgattggga ggaacatttg gctgtcaaac acttctctgt tgaaggtcaa ctcgaattca aggccatctt gttcgttcca aagcgtgctc catttgattt gttcgaacca aagaagaagt tgaacaatat caaactctac gtgagaagag tctttattat ggacaattgt gaagaattaa ttccagaata cttgaacttt attaagggta ttgttgacag tgaggacttg ccattgaaca tttctcgtga gcacttgcaa caaaacaaga ttatgaaggt gattagaaag aacttggtca agaagtgcat tgaaatgttc tctgaaattg ctgaaaacaa ggatgatttc aaggcctttt atgaatcctt tggtaagaac ttgaagttgg gaattcacga agactctcaa aacagacaaa agttggctga attgctcaga ttccactcat ccacttcggg cgaggaatgg acaagcctta aggaatatgt ggaaagaatg aaggaaggtc aagaagacat ttactacatt actggtgaaa gcaagaaggc tgtctccaaa tctccattca ttgaaaagtg taccaagaag ggctttgaag tcttgtacat gactgatcct attgatgaat acatggttca acaattgaag gaatttgatg gtaaaaagtt ggtctgtgtc accaaggaag gtttgaagtt gccagaaact gaagaggaaa agaagaagaa ggaagaattg aaggcctctt tcgaatcctt gtgcaagttg atgaaggaca ttttgggtga caaggtcgag aaagtggttg tttccgatag acttggtgac tctccatgtt gcttggtcac tggtgaatat ggatggagtg caaacatgga aagaatcatg aaggctcaag ctttgaagga taactcaatg gctgcttaca tggtttctaa aaagactatg gaaattaacc cagaaaactc gatcgtcaat gaattgagaa agaaggctga ggctaacaag gctgacaaga ctttgagaga tttggtctgg ctcttgtttg acacttcctt gttgacttct ggattctcat tggaggaacc aagcatttat gcttctcgta ttcacagaat gatcaaactt ggttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.17129.a.B4.GE40628
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 77kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHTY KFQAEINQLM SLIINTFYSN KEIFLRELIS NASDALDKIR HLSLTDPSVL DTEKELYIRI IPDKTNKTLT IQDSGVGMTK ADLINCLGTI ARSGTKQFME MLQAGADISL IGQFGVGFYS AYLVADRVVV VTKHNDDEQY IWESAAGGSF TITLDESGEK MTRGTKIILH MKEDQLEYLE ERRLKDLVKK HSEFIGYPIS LYVEKTSEKE VEEEEEPEEK EDKEEPKIEE VDEEKEKKKK KVKEVTHEWE LLNKTKPLWT RDPKSITKEE YASFYKALTN DWEEHLAVKH FSVEGQLEFK AILFVPKRAP FDLFEPKKKL NNIKLYVRRV FIMDNCEELI PEYLNFIKGI VDSEDLPLNI SREHLQQNKI MKVIRKNLVK KCIEMFSEIA ENKDDFKAFY ESFGKNLKLG IHEDSQNRQK LAELLRFHSS TSGEEWTSLK EYVERMKEGQ EDIYYITGES KKAVSKSPFI EKCTKKGFEV LYMTDPIDEY MVQQLKEFDG KKLVCVTKEG LKLPETEEEK KKKEELKASF ESLCKLMKDI LGDKVEKVVV SDRLGDSPCC LVTGEYGWSA NMERIMKAQA LKDNSMAAYM VSKKTMEINP ENSIVNELRK KAEANKADKT LRDLVWLLFD TSLLTSGFSL EEPSIYASRI HRMIKLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacctaca aattccaagc tgaaatcaat caattgatga gtttaatcat caatactttt tatagcaata aggaaatctt cttgagagag ttaatttcca atgcttctga tgctttggat aaaattcgcc acttgagttt gactgaccct tcagtcttgg acactgaaaa agaattgtat attcgtatca ttccagataa gactaacaag acattgacca ttcaagattc aggtgttggt atgacaaaag ctgatttaat taattgtctt ggtacgatcg ccagatccgg tacaaaacaa ttcatggaaa tgcttcaagc aggagctgac atttctttga ttggtcaatt cggtgtcggt ttttacagtg cttacttggt tgcagatcga gtggttgtag tgaccaagca caatgatgac gaacaataca tttgggaatc tgctgctggt ggtagcttta ccattacttt ggatgagagt ggtgaaaaaa tgactcgtgg taccaagatt atcttgcaca tgaaggaaga tcaattggaa tatttggagg aacgtcgatt gaaggatctt gtcaagaagc actctgaatt tattggttat ccaatctcct tgtacgtcga aaagaccagc gaaaaggagg tggaagaaga agaagaacct gaagaaaagg aggacaagga agaaccaaag attgaagaag tcgacgaaga gaaggaaaag aagaagaaga aggtgaagga ggtgacccat gaatgggaat tgctcaacaa gaccaagcct ctctggactc gtgacccaaa gagcatcacc aaggaagaat atgcaagctt ttacaaggct ttgaccaacg attgggagga acatttggct gtcaaacact tctctgttga aggtcaactc gaattcaagg ccatcttgtt cgttccaaag cgtgctccat ttgatttgtt cgaaccaaag aagaagttga acaatatcaa actctacgtg agaagagtct ttattatgga caattgtgaa gaattaattc cagaatactt gaactttatt aagggtattg ttgacagtga ggacttgcca ttgaacattt ctcgtgagca cttgcaacaa aacaagatta tgaaggtgat tagaaagaac ttggtcaaga agtgcattga aatgttctct gaaattgctg aaaacaagga tgatttcaag gccttttatg aatcctttgg taagaacttg aagttgggaa ttcacgaaga ctctcaaaac agacaaaagt tggctgaatt gctcagattc cactcatcca cttcgggcga ggaatggaca agccttaagg aatatgtgga aagaatgaag gaaggtcaag aagacattta ctacattact ggtgaaagca agaaggctgt ctccaaatct ccattcattg aaaagtgtac caagaagggc tttgaagtct tgtacatgac tgatcctatt gatgaataca tggttcaaca attgaaggaa tttgatggta aaaagttggt ctgtgtcacc aaggaaggtt tgaagttgcc agaaactgaa gaggaaaaga agaagaagga agaattgaag gcctctttcg aatccttgtg caagttgatg aaggacattt tgggtgacaa ggtcgagaaa gtggttgttt ccgatagact tggtgactct ccatgttgct tggtcactgg tgaatatgga tggagtgcaa acatggaaag aatcatgaag gctcaagctt tgaaggataa ctcaatggct gcttacatgg tttctaaaaa gactatggaa attaacccag aaaactcgat cgtcaatgaa ttgagaaaga aggctgaggc taacaaggct gacaagactt tgagagattt ggtctggctc ttgtttgaca cttccttgtt gacttctgga ttctcattgg aggaaccaag catttatgct tctcgtattc acagaatgat caaacttggt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NafoA.17129.a.B2.PW37999
PURIFICATION DATe: 12/19/2016
CONCENTRATION: 30.7mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT YKFQAEINQL MSLIINTFYS NKEIFLRELI SNASDALDKI RHLSLTDPSV LDTEKELYIR IIPDKTNKTL TIQDSGVGMT KADLINCLGT IARSGTKQFM EMLQAGADIS LIGQFGVGFY SAYLVADRVV VVTKHNDDEQ YIWESAAGGS FTITLDESGE KMTRGTKIIL HMKEDQLEYL EERRLKDLVK KHSEFIGYPI SLYVE
Validated NT Sequence
atcttactca cttattcgac gtacaaggag attggataac caataaattc agagtgcttc ttgacaagat ccttcaatcg acgttcctcc aaatattcca attgatcttc cttcatgtgc aagataatct tggtaccacg agtcattttt tcaccactct catccaaagt aatggtaaag ctaccaccag cagcagattc ccaaatgtat tgttcgtcat cattgtgctt ggtcactaca accactcgat ctgcaaccaa gtaagcactg taaaaaccga caccgaattg accaatcaaa gaaatgtcag ctcctgcttg aagcatttcc atgaattgtt ttgtaccgga tctggcgatc gtaccaagac aattaattaa atcagctttt gtcataccaa cacctgaatc ttgaatggtc aatgtcttgt tagtcttatc tggaatgata cgaatataca attctttttc agtgtccaag actgaagggt cagtcaaact caagtggcga attttatcca aagcatcaga agcattggaa attaactctc tcaagaagat ttccttattg ctataaaaag tattgatgat taaactcatc aattgattga tttcagcttg gaatttgtag gtcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHTY KFQAEINQLM SLIINTFYSN KEIFLRELIS NASDALDKIR HLSLTDPSVL DTEKELYIRI IPDKTNKTLT IQDSGVGMTK ADLINCLGTI ARSGTKQFME MLQAGADISL IGQFGVGFYS AYLVADRVVV VTKHNDDEQY IWESAAGGSF TITLDESGEK MTRGTKIILH MKEDQLEYLE ERRLKDLVKK HSEFIGYPIS LYVE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATACCTACA AATTCCAAGC TGAAATCAAT CAATTGATGA GTTTAATCAT CAATACTTTT TATAGCAATA AGGAAATCTT CTTGAGAGAG TTAATTTCCA ATGCTTCTGA TGCTTTGGAT AAAATTCGCC ACTTGAGTTT GACTGACCCT TCAGTCTTGG ACACTGAAAA AGAATTGTAT ATTCGTATCA TTCCAGATAA GACTAACAAG ACATTGACCA TTCAAGATTC AGGTGTTGGT ATGACAAAAG CTGATTTAAT TAATTGTCTT GGTACGATCG CCAGATCCGG TACAAAACAA TTCATGGAAA TGCTTCAAGC AGGAGCTGAC ATTTCTTTGA TTGGTCAATT CGGTGTCGGT TTTTACAGTG CTTACTTGGT TGCAGATCGA GTGGTTGTAG TGACCAAGCA CAATGATGAC GAACAATACA TTTGGGAATC TGCTGCTGGT GGTAGCTTTA CCATTACTTT GGATGAGAGT GGTGAAAAAA TGACTCGTGG TACCAAGATT ATCTTGCACA TGAAGGAAGA TCAATTGGAA TATTTGGAGG AACGTCGATT GAAGGATCTT GTCAAGAAGC ACTCTGAATT TATTGGTTAT CCAATCTCCT TGTACGTCGA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for NafoA.17129.a.B3.PW38000
PURIFICATION DATe: 12/19/2016
CONCENTRATION: 16mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHLW TRDPKSITKE EYASFYKALT NDWEEHLAVK HFSVEGQLEF KAILFVPKRA PFDLFEPKKK LNNIKLYVRR VFIMDNCEEL IPEYLNFIKG IVDSEDLPLN ISREHLQQNK IMKVIRKNLV KKCIEMFSEI AENKDDFKAF YESFGKNLKL GIHEDSQNRQ KLAELLRFHS STSGEEWTSL KEYVERMKEG QEDIYYITGE SKKAVSKSPF IEKCTKKGFE VLYMTDPIDE YMVQQLKEFD GKKLVCVTKE GLKLPETEEE KKKKEELKAS FESLCKLMKD ILGDKVEKVV VSDRLGDSPC CLVTGEYGWS ANMERIMKAQ ALKDNSMAAY MVSKKTMEIN PENSIVNELR KKAEANKADK TLRDLVWLLF DTSLLTSGFS LEEPSIYASR IHRMIKLG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTCTGGA CTCGTGACCC AAAGAGCATC ACCAAGGAAG AATATGCAAG CTTTTACAAG GCTTTGACCA ACGATTGGGA GGAACATTTG GCTGTCAAAC ACTTCTCTGT TGAAGGTCAA CTCGAATTCA AGGCCATCTT GTTCGTTCCA AAGCGTGCTC CATTTGATTT GTTCGAACCA AAGAAGAAGT TGAACAATAT CAAACTCTAC GTGAGAAGAG TCTTTATTAT GGACAATTGT GAAGAATTAA TTCCAGAATA CTTGAACTTT ATTAAGGGTA TTGTTGACAG TGAGGACTTG CCATTGAACA TTTCTCGTGA GCACTTGCAA CAAAACAAGA TTATGAAGGT GATTAGAAAG AACTTGGTCA AGAAGTGCAT TGAAATGTTC TCTGAAATTG CTGAAAACAA GGATGATTTC AAGGCCTTTT ATGAATCCTT TGGTAAGAAC TTGAAGTTGG GAATTCACGA AGACTCTCAA AACAGACAAA AGTTGGCTGA ATTGCTCAGA TTCCACTCAT CCACTTCGGG CGAGGAATGG ACAAGCCTTA AGGAATATGT GGAAAGAATG AAGGAAGGTC AAGAAGACAT TTACTACATT ACTGGTGAAA GCAAGAAGGC TGTCTCCAAA TCTCCATTCA TTGAAAAGTG TACCAAGAAG GGCTTTGAAG TCTTGTACAT GACTGATCCT ATTGATGAAT ACATGGTTCA ACAATTGAAG GAATTTGATG GTAAAAAGTT GGTCTGTGTC ACCAAGGAAG GTTTGAAGTT GCCAGAAACT GAAGAGGAAA AGAAGAAGAA GGAAGAATTG AAGGCCTCTT TCGAATCCTT GTGCAAGTTG ATGAAGGACA TTTTGGGTGA CAAGGTCGAG AAAGTGGTTG TTTCCGATAG ACTTGGTGAC TCTCCATGTT GCTTGGTCAC TGGTGAATAT GGATGGAGTG CAAACATGGA AAGAATCATG AAGGCTCAAG CTTTGAAGGA TAACTCAATG GCTGCTTACA TGGTTTCTAA AAAGACTATG GAAATTAACC CAGAAAACTC GATCGTCAAT GAATTGAGAA AGAAGGCTGA GGCTAACAAG GCTGACAAGA CTTTGAGAGA TTTGGTCTGG CTCTTGTTTG ACACTTCCTT GTTGACTTCT GGATTCTCAT TGGAGGAACC AAGCATTTAT GCTTCTCGTA TTCACAGAAT GATCAAACTT GGTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT