NafoA.01637.a

serine hydroxymethyltransferase

CENTER ID: NafoA.01637.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01637.a.B1.GE40546 Full length(NafoA.01637.a) 1 462
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01637.a.B1.PS38370 Full length(NafoA.01637.a) 1 462

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0009170

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSSHQANIP KLVGNTPLSE ADPELFELIE KEKNRQWKGL ELIASENFTS QAVMECLGSC LTNKYSEGQV GARYYGGNEV IDEIEKLCKE RALAAFDLSS EEWGVNVQPY SGSPANFAVY TGLLQPHDRI MGLDLPSGGH LTHGYYSGKK KISATSIFFE SLPYCVNEQG YIDYDGLEKS AKVFRPKLII CGGSAYPRDW DYARLRKIAS DVEAYLMCDM AHYSGLVAAK EHNNPFEFCD VVTSTTHKSL RGPRAGIIFA KKELMPKIDF AVFPGIQGGP HNHQIAAIAT QLKQVQTPEY KEYIKQVKAN AKTLAKCLID YGYTLATGGT DNHLVLWNLR PQGITGSKME KLFDAVSITA NKNSIAGDPN ALSPYGIRLG TPALTTRGFK EEDFVLVAEF LHRGLKLGLR LQQQASSPKL QDFLAQINPE DEELKQLKHD VETFAKQFGI PGWNIEEMKF RD
NT Sequence
ATGTCTTCCT CTCATCAAGC CAACATTCCC AAACTCGTCG GAAATACACC ACTCTCCGAA GCAGATCCAG AACTCTTTGA ACTCATTGAA AAGGAAAAGA ATAGACAATG GAAGGGTCTT GAATTAATTG CTAGTGAAAA TTTTACAAGC CAAGCCGTGA TGGAATGTCT CGGAAGTTGC CTTACCAACA AGTATAGCGA AGGACAAGTG GGTGCTCGTT ACTATGGAGG AAATGAAGTG ATTGATGAAA TTGAAAAGTT GTGCAAGGAG AGAGCTCTTG CTGCTTTTGA TCTCTCATCA GAGGAATGGG GAGTCAATGT TCAACCCTAC TCCGGATCAC CTGCAAACTT TGCAGTTTAT ACTGGTCTGT TGCAACCTCA TGATCGTATC ATGGGTCTTG ATTTACCAAG CGGAGGACAT TTAACTCACG GATATTATTC TGGAAAGAAG AAAATTTCTG CAACTTCCAT CTTCTTTGAA AGTTTACCCT ATTGTGTGAA TGAACAGGGT TATATTGATT ATGATGGACT TGAGAAATCA GCAAAAGTTT TTAGACCAAA ATTAATTATT TGTGGAGGAA GTGCCTATCC AAGAGATTGG GATTATGCCA GATTGAGAAA GATTGCTTCC GATGTTGAAG CCTATTTAAT GTGTGATATG GCTCACTATT CTGGATTGGT TGCTGCCAAA GAACACAATA ATCCATTCGA ATTTTGCGAT GTTGTCACAT CAACCACACA CAAATCACTC CGAGGACCTA GAGCTGGTAT CATATTCGCC AAGAAGGAAC TCATGCCAAA GATTGATTTT GCAGTATTCC CTGGCATTCA AGGTGGTCCA CACAATCATC AAATTGCTGC GATCGCCACA CAATTGAAAC AAGTTCAAAC ACCAGAATAC AAGGAATATA TCAAGCAAGT TAAAGCCAAT GCCAAGACGC TCGCCAAATG TCTCATTGAC TATGGATACA CATTGGCGAC TGGAGGCACT GACAATCATT TGGTGTTGTG GAATTTGAGA CCACAAGGAA TCACTGGAAG CAAGATGGAA AAGCTTTTTG ATGCTGTCTC CATCACGGCT AACAAGAACT CGATTGCTGG AGATCCAAAC GCGTTGTCAC CTTATGGAAT TCGTTTGGGG ACTCCTGCCC TGACCACTCG TGGATTCAAG GAAGAAGATT TTGTTCTGGT TGCTGAATTC TTGCATCGTG GCTTGAAATT GGGATTGAGA CTCCAACAAC AAGCTTCCTC TCCAAAATTG CAAGACTTTT TGGCTCAAAT TAACCCAGAG GATGAGGAGT TGAAGCAATT GAAGCATGAT GTGGAAACAT TCGCTAAACA GTTCGGAATC CCTGGATGGA ATATTGAAGA AATGAAATTT AGAGAT
Details for NafoA.01637.a.B1.GE40546
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 50
Validated AA Sequence
MCDMAHYSGL VAAKEHNNPF EFCDVVTSTT HKSLRGPRAG IIFAKKELMP KIDFAVFPGI QGGPHNHQIA AIATQLKQVQ TPEYKEYIKQ VKANAKTLAK CLIDYGYTLA TGGTDNHLVL WNLRPQGITG SKMEKLFDAV SITANKNSIA GDPNALSPYG IRLGTPALTT RGFKEEDFVL VAEFLHRGLK LGLRLQQQAS SPKLQDFLAQ INPEDEELKQ LKHDVETFAK QFGIPGWN
Validated NT Sequence
caccannnnn nnnannnnnn nncntcnnnn nnnnnnnnnn ntcnnnnnnn nntnnnnnnn annncnnnnn nnnnnnnnng atccnnnnnt ctttgnnnnn nnnnnnnngg aaaagaatnn nnnnnnnnnn ngtcttgaan nnnntgctag tgaaaatttn nnnngccaag ccgtgatgga ntnnnncgga agttgcctta ccaacaagta tagcnnntgg acannngggt gctcgttact atggnggaaa tgaagtgatt gatgaaattg aaaagttgtg caaggagaga gctcttgctg cttttgannn ctcatcagnn naatggggan nnannnnnna accctactcc ggatcacctn nnaactttgc agtttatact ggtctgttgc aacctcatga tcgtatcatg ggtcttgatt tannnnnnnn aggacattta actcacggat atnnnnntgn nnnnnnnnna aaatttctgc aacttccatn nnctttgaaa gtttacccta ttgtgtgaat gaacagggtt atattgatta tgatggactt gagnaatcag caaaagtttt tagaccaaan nnnnntattt gtggagnnag tgcctatcnn nnagattggg attatgccag attgagaaag attgcttccg atgttgaagc ctatttaatg tgtgatatgg ctcactattc tggattggtt gctgccaaag aacacaataa tccattcgaa ttttgcgatg ttgtcacatc aaccacacac aaatcactcc gaggacctag agctggtatc atattcgcca agaaggaact catgccaaag attgattttg cagtattccc tggcattcaa ggtggtccac acaatcatca aattgctgcg atcgccacac aattgaaaca agttcaaaca ccagaataca aggaatatat caagcaagtt aaagccaatg ccaagacgct cgccaaatgt ctcattgact atggatacac attggcgact ggaggcactg acaatcattt ggtgttgtgg aatttgagac cacaaggaat cactggaagc aagatggaaa agctttttga tgctgtctcc atcacggcta acaagaactc gattgctgga gatccaaacg cgttgtcacc ttatggaatt cgtttgggga ctcctgccct gaccactcgt ggattcaagg aagaagattt tgttctggtt gctgaattct tgcatcgtgg cttgaaattg ggattgagac tccaacaaca agcttcctct ccaaaattgc aagacttttt ggctcaaatt aacccagagg atgaggagtt gaagcaattg aagcatgatg tggaaacatt cgctaaacag ttcggaatcc ctggatggaa ta
Expected Protein Sequence
MAHHHHHHMS SSHQANIPKL VGNTPLSEAD PELFELIEKE KNRQWKGLEL IASENFTSQA VMECLGSCLT NKYSEGQVGA RYYGGNEVID EIEKLCKERA LAAFDLSSEE WGVNVQPYSG SPANFAVYTG LLQPHDRIMG LDLPSGGHLT HGYYSGKKKI SATSIFFESL PYCVNEQGYI DYDGLEKSAK VFRPKLIICG GSAYPRDWDY ARLRKIASDV EAYLMCDMAH YSGLVAAKEH NNPFEFCDVV TSTTHKSLRG PRAGIIFAKK ELMPKIDFAV FPGIQGGPHN HQIAAIATQL KQVQTPEYKE YIKQVKANAK TLAKCLIDYG YTLATGGTDN HLVLWNLRPQ GITGSKMEKL FDAVSITANK NSIAGDPNAL SPYGIRLGTP ALTTRGFKEE DFVLVAEFLH RGLKLGLRLQ QQASSPKLQD FLAQINPEDE ELKQLKHDVE TFAKQFGIPG WNIEEMKFRD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt cctctcatca agccaacatt cccaaactcg tcggaaatac accactctcc gaagcagatc cagaactctt tgaactcatt gaaaaggaaa agaatagaca atggaagggt cttgaattaa ttgctagtga aaattttaca agccaagccg tgatggaatg tctcggaagt tgccttacca acaagtatag cgaaggacaa gtgggtgctc gttactatgg aggaaatgaa gtgattgatg aaattgaaaa gttgtgcaag gagagagctc ttgctgcttt tgatctctca tcagaggaat ggggagtcaa tgttcaaccc tactccggat cacctgcaaa ctttgcagtt tatactggtc tgttgcaacc tcatgatcgt atcatgggtc ttgatttacc aagcggagga catttaactc acggatatta ttctggaaag aagaaaattt ctgcaacttc catcttcttt gaaagtttac cctattgtgt gaatgaacag ggttatattg attatgatgg acttgagaaa tcagcaaaag tttttagacc aaaattaatt atttgtggag gaagtgccta tccaagagat tgggattatg ccagattgag aaagattgct tccgatgttg aagcctattt aatgtgtgat atggctcact attctggatt ggttgctgcc aaagaacaca ataatccatt cgaattttgc gatgttgtca catcaaccac acacaaatca ctccgaggac ctagagctgg tatcatattc gccaagaagg aactcatgcc aaagattgat tttgcagtat tccctggcat tcaaggtggt ccacacaatc atcaaattgc tgcgatcgcc acacaattga aacaagttca aacaccagaa tacaaggaat atatcaagca agttaaagcc aatgccaaga cgctcgccaa atgtctcatt gactatggat acacattggc gactggaggc actgacaatc atttggtgtt gtggaatttg agaccacaag gaatcactgg aagcaagatg gaaaagcttt ttgatgctgt ctccatcacg gctaacaaga actcgattgc tggagatcca aacgcgttgt caccttatgg aattcgtttg gggactcctg ccctgaccac tcgtggattc aaggaagaag attttgttct ggttgctgaa ttcttgcatc gtggcttgaa attgggattg agactccaac aacaagcttc ctctccaaaa ttgcaagact ttttggctca aattaaccca gaggatgagg agttgaagca attgaagcat gatgtggaaa cattcgctaa acagttcgga atccctggat ggaatattga agaaatgaaa tttagagatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for NafoA.01637.a.B1.PS38370
PURIFICATION DATe: 10/24/2017
CONCENTRATION: 18.5mg/ml
OBSERVED MW: 50kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 50
Protocol Notes
notes unavailable
Validated AA Sequence
MCDMAHYSGL VAAKEHNNPF EFCDVVTSTT HKSLRGPRAG IIFAKKELMP KIDFAVFPGI QGGPHNHQIA AIATQLKQVQ TPEYKEYIKQ VKANAKTLAK CLIDYGYTLA TGGTDNHLVL WNLRPQGITG SKMEKLFDAV SITANKNSIA GDPNALSPYG IRLGTPALTT RGFKEEDFVL VAEFLHRGLK LGLRLQQQAS SPKLQDFLAQ INPEDEELKQ LKHDVETFAK QFGIPGWN
Validated NT Sequence
caccannnnn nnnannnnnn nncntcnnnn nnnnnnnnnn ntcnnnnnnn nntnnnnnnn annncnnnnn nnnnnnnnng atccnnnnnt ctttgnnnnn nnnnnnnngg aaaagaatnn nnnnnnnnnn ngtcttgaan nnnntgctag tgaaaatttn nnnngccaag ccgtgatgga ntnnnncgga agttgcctta ccaacaagta tagcnnntgg acannngggt gctcgttact atggnggaaa tgaagtgatt gatgaaattg aaaagttgtg caaggagaga gctcttgctg cttttgannn ctcatcagnn naatggggan nnannnnnna accctactcc ggatcacctn nnaactttgc agtttatact ggtctgttgc aacctcatga tcgtatcatg ggtcttgatt tannnnnnnn aggacattta actcacggat atnnnnntgn nnnnnnnnna aaatttctgc aacttccatn nnctttgaaa gtttacccta ttgtgtgaat gaacagggtt atattgatta tgatggactt gagnaatcag caaaagtttt tagaccaaan nnnnntattt gtggagnnag tgcctatcnn nnagattggg attatgccag attgagaaag attgcttccg atgttgaagc ctatttaatg tgtgatatgg ctcactattc tggattggtt gctgccaaag aacacaataa tccattcgaa ttttgcgatg ttgtcacatc aaccacacac aaatcactcc gaggacctag agctggtatc atattcgcca agaaggaact catgccaaag attgattttg cagtattccc tggcattcaa ggtggtccac acaatcatca aattgctgcg atcgccacac aattgaaaca agttcaaaca ccagaataca aggaatatat caagcaagtt aaagccaatg ccaagacgct cgccaaatgt ctcattgact atggatacac attggcgact ggaggcactg acaatcattt ggtgttgtgg aatttgagac cacaaggaat cactggaagc aagatggaaa agctttttga tgctgtctcc atcacggcta acaagaactc gattgctgga gatccaaacg cgttgtcacc ttatggaatt cgtttgggga ctcctgccct gaccactcgt ggattcaagg aagaagattt tgttctggtt gctgaattct tgcatcgtgg cttgaaattg ggattgagac tccaacaaca agcttcctct ccaaaattgc aagacttttt ggctcaaatt aacccagagg atgaggagtt gaagcaattg aagcatgatg tggaaacatt cgctaaacag ttcggaatcc ctggatggaa ta
Expressed Protein Sequence
MAHHHHHHMS SSHQANIPKL VGNTPLSEAD PELFELIEKE KNRQWKGLEL IASENFTSQA VMECLGSCLT NKYSEGQVGA RYYGGNEVID EIEKLCKERA LAAFDLSSEE WGVNVQPYSG SPANFAVYTG LLQPHDRIMG LDLPSGGHLT HGYYSGKKKI SATSIFFESL PYCVNEQGYI DYDGLEKSAK VFRPKLIICG GSAYPRDWDY ARLRKIASDV EAYLMCDMAH YSGLVAAKEH NNPFEFCDVV TSTTHKSLRG PRAGIIFAKK ELMPKIDFAV FPGIQGGPHN HQIAAIATQL KQVQTPEYKE YIKQVKANAK TLAKCLIDYG YTLATGGTDN HLVLWNLRPQ GITGSKMEKL FDAVSITANK NSIAGDPNAL SPYGIRLGTP ALTTRGFKEE DFVLVAEFLH RGLKLGLRLQ QQASSPKLQD FLAQINPEDE ELKQLKHDVE TFAKQFGIPG WNIEEMKFRD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTT CCTCTCATCA AGCCAACATT CCCAAACTCG TCGGAAATAC ACCACTCTCC GAAGCAGATC CAGAACTCTT TGAACTCATT GAAAAGGAAA AGAATAGACA ATGGAAGGGT CTTGAATTAA TTGCTAGTGA AAATTTTACA AGCCAAGCCG TGATGGAATG TCTCGGAAGT TGCCTTACCA ACAAGTATAG CGAAGGACAA GTGGGTGCTC GTTACTATGG AGGAAATGAA GTGATTGATG AAATTGAAAA GTTGTGCAAG GAGAGAGCTC TTGCTGCTTT TGATCTCTCA TCAGAGGAAT GGGGAGTCAA TGTTCAACCC TACTCCGGAT CACCTGCAAA CTTTGCAGTT TATACTGGTC TGTTGCAACC TCATGATCGT ATCATGGGTC TTGATTTACC AAGCGGAGGA CATTTAACTC ACGGATATTA TTCTGGAAAG AAGAAAATTT CTGCAACTTC CATCTTCTTT GAAAGTTTAC CCTATTGTGT GAATGAACAG GGTTATATTG ATTATGATGG ACTTGAGAAA TCAGCAAAAG TTTTTAGACC AAAATTAATT ATTTGTGGAG GAAGTGCCTA TCCAAGAGAT TGGGATTATG CCAGATTGAG AAAGATTGCT TCCGATGTTG AAGCCTATTT AATGTGTGAT ATGGCTCACT ATTCTGGATT GGTTGCTGCC AAAGAACACA ATAATCCATT CGAATTTTGC GATGTTGTCA CATCAACCAC ACACAAATCA CTCCGAGGAC CTAGAGCTGG TATCATATTC GCCAAGAAGG AACTCATGCC AAAGATTGAT TTTGCAGTAT TCCCTGGCAT TCAAGGTGGT CCACACAATC ATCAAATTGC TGCGATCGCC ACACAATTGA AACAAGTTCA AACACCAGAA TACAAGGAAT ATATCAAGCA AGTTAAAGCC AATGCCAAGA CGCTCGCCAA ATGTCTCATT GACTATGGAT ACACATTGGC GACTGGAGGC ACTGACAATC ATTTGGTGTT GTGGAATTTG AGACCACAAG GAATCACTGG AAGCAAGATG GAAAAGCTTT TTGATGCTGT CTCCATCACG GCTAACAAGA ACTCGATTGC TGGAGATCCA AACGCGTTGT CACCTTATGG AATTCGTTTG GGGACTCCTG CCCTGACCAC TCGTGGATTC AAGGAAGAAG ATTTTGTTCT GGTTGCTGAA TTCTTGCATC GTGGCTTGAA ATTGGGATTG AGACTCCAAC AACAAGCTTC CTCTCCAAAA TTGCAAGACT TTTTGGCTCA AATTAACCCA GAGGATGAGG AGTTGAAGCA ATTGAAGCAT GATGTGGAAA CATTCGCTAA ACAGTTCGGA ATCCCTGGAT GGAATATTGA AGAAATGAAA TTTAGAGATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT