SSGCID: Target Page

NafoA.01592.a

rab family small gtpase

CENTER ID:	NafoA.01592.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.01592.a.B1.GE40543	Full length(NafoA.01592.a)		1	207

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0117600

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASRKELLKI IVLGDSGVGK TSLMNQYVNN KFSNQYKATI GADFLTKEIM IEDTCCSLSI WDTAGQERFH SLGFAFFRGS DACVLVFDAT TQKTFDNINN WRDEFLLQTG PSDPENFPFV LIGNKIDLEQ QRAVQQKQAL AWCKSKGNIP YFECSAKDGT NVEHAFVTVA RKALANAKDS NGVTEGANTL GNIDDEPTPR PNNGCAC

NT Sequence

ATGGCTTCCC GCAAAGAGTT ATTGAAAATC ATTGTCCTCG GAGATTCGGG TGTTGGAAAG ACTTCTCTCA TGAATCAATA CGTTAATAAC AAGTTCAGCA ATCAATATAA AGCAACCATT GGAGCAGATT TTTTGACAAA GGAAATTATG ATTGAAGATA CATGTTGTTC TTTATCCATT TGGGATACTG CAGGACAAGA ACGATTTCAC TCATTGGGAT TTGCTTTTTT CAGAGGTTCA GATGCTTGTG TATTAGTATT TGACGCAACC ACTCAAAAAA CATTTGATAA TATTAATAAT TGGAGAGATG AATTCTTGTT ACAAACAGGA CCATCAGATC CTGAGAATTT TCCATTTGTA TTGATTGGAA ACAAGATTGA TTTGGAGCAA CAAAGAGCTG TGCAACAAAA ACAGGCACTC GCATGGTGCA AGAGTAAAGG CAATATTCCA TACTTTGAAT GCAGTGCAAA AGATGGCACC AATGTTGAGC ATGCTTTTGT GACGGTGGCT CGAAAGGCCC TTGCAAATGC TAAAGATTCA AATGGTGTCA CAGAAGGAGC AAATACTTTA GGTAACATTG ATGACGAGCC AACTCCAAGA CCAAATAATG GATGTGCATG C

Details for NafoA.01592.a.B1.GE40543

HARVESTED ON:	9/1/2016
SEQUENCED ON:	9/8/2016
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA SRKELLKIIV LGDSGVGKTS LMNQYVNNKF SNQYKATIGA DFLTKEIMIE DTCCSLSIWD TAGQERFHSL GFAFFRGSDA CVLVFDATTQ KTFDNINNWR DEFLLQTGPS DPENFPFVLI GNKIDLEQQR AVQQKQALAW CKSKGNIPYF ECSAKDGTNV EHAFVTVARK ALANAKDSNG VTEGANTLGN IDDEPTPRPN NGCAC

Validated NT Sequence

atggctcacc accaccacca ccatatggct tcccgcaaag agttattgaa aatcattgtc ctcggagatt cgggtgttgg aaagacttct ctcatgaatc aatacgttaa taacaagttc agcaatcaat ataaagcaac cattggagca gattttttga caaaggaaat tatgattgaa gatacatgtt gttctttatc catttgggat actgcaggac aagaacgatt tcactcattg ggatttgctt ttttcagagg ttcagatgct tgtgtattag tatttgacgc aaccactcaa aaaacatttg ataatattaa taattggaga gatgaattct tgttacaaac aggaccatca gatcctgaga attttccatt tgtattgatt ggaaacaaga ttgatttgga gcaacaaaga gctgtgcaac aaaaacaggc actcgcatgg tgcaagagta aaggcaatat tccatacttt gaatgcagtg caaaagatgg caccaatgtt gagcatgctt ttgtgacggt ggctcgaaag gcccttgcaa atgctaaaga ttcaaatggt gtcacagaag gagcaaatac tttaggtaac attgatgacg agccaactcc aagaccaaat aatggatgtg catgctaagt gagtaagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctt cccgcaaaga gttattgaaa atcattgtcc tcggagattc gggtgttgga aagacttctc tcatgaatca atacgttaat aacaagttca gcaatcaata taaagcaacc attggagcag attttttgac aaaggaaatt atgattgaag atacatgttg ttctttatcc atttgggata ctgcaggaca agaacgattt cactcattgg gatttgcttt tttcagaggt tcagatgctt gtgtattagt atttgacgca accactcaaa aaacatttga taatattaat aattggagag atgaattctt gttacaaaca ggaccatcag atcctgagaa ttttccattt gtattgattg gaaacaagat tgatttggag caacaaagag ctgtgcaaca aaaacaggca ctcgcatggt gcaagagtaa aggcaatatt ccatactttg aatgcagtgc aaaagatggc accaatgttg agcatgcttt tgtgacggtg gctcgaaagg cccttgcaaa tgctaaagat tcaaatggtg tcacagaagg agcaaatact ttaggtaaca ttgatgacga gccaactcca agaccaaata atggatgtgc atgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat