NafoA.01586.a

ATP-dependent RNA helicase eIF4A

CENTER ID: NafoA.01586.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01586.a.B1.GE40542 Full length(NafoA.01586.a) 1 407
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01586.a.B1.PW37978 Full length(NafoA.01586.a) 1 407

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0007150

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVNNDITVTV DDDYKQKHGK ARYEQKEVKE FDDMNLKEDL LRGIYSYGFE KPSIIQQRAI LPITTSEPPA DVIGQAQSGT GKTATFTISM LQRLNISPEV TQPQGLVLAP TRELAQQIHK VIMSLGEYMK VKVHACVGGT KVQHDIAILE EGVHIIVGTP GRVFHMIQSG HLDVSGIKML VIDEADEMLS RGFKDQIYAI FKNLPQDMQV CLFSATMPAD VLEITDKFMR DPIRILVKKE ELTLEGIKQF YIMCEKEDYK LDTLCDLYKI LTISQCVIFC NSRKKVEYLA EQLNKRDFTV SSMHGDMEPK DRELIMHEFR TSASRVLITT DLLARGIDVH HVSLVINYDL PRHKENYLHR IGRSGRYGRK GVAINFITKD DIRSMREIEK FYSTSIEECP QNIHELI
NT Sequence
ATGGTTAATA ACGATATCAC TGTTACTGTC GATGATGACT ACAAGCAAAA GCACGGAAAG GCAAGATATG AACAAAAGGA AGTCAAAGAA TTTGATGATA TGAATCTCAA GGAAGACTTG TTGAGAGGAA TTTACTCTTA CGGTTTTGAA AAGCCTTCTA TCATCCAACA ACGTGCTATT TTGCCCATCA CTACCTCTGA ACCACCAGCA GATGTTATCG GTCAAGCCCA ATCTGGTACC GGTAAGACTG CTACCTTCAC TATTTCCATG CTTCAAAGAT TGAATATTTC TCCTGAAGTA ACACAACCAC AAGGACTCGT GTTGGCTCCA ACCCGTGAGT TGGCACAACA AATCCACAAG GTAATCATGT CCTTGGGTGA ATACATGAAA GTTAAGGTTC ACGCTTGTGT CGGTGGTACT AAAGTACAAC ACGATATTGC TATTCTTGAA GAAGGAGTGC ATATTATTGT TGGTACTCCT GGACGTGTCT TCCACATGAT TCAAAGTGGA CACTTGGATG TTAGCGGTAT TAAAATGTTG GTCATTGATG AAGCTGACGA AATGTTGAGT CGTGGTTTCA AGGACCAAAT CTATGCTATT TTCAAGAACC TTCCTCAAGA TATGCAAGTC TGTTTGTTCA GCGCTACTAT GCCTGCTGAT GTTTTGGAAA TTACTGACAA GTTTATGAGA GATCCAATTA GAATCTTGGT CAAGAAGGAA GAATTGACTT TGGAAGGTAT CAAGCAATTC TATATTATGT GTGAAAAGGA AGATTACAAG TTGGACACAT TGTGTGACTT GTACAAGATT TTGACCATTT CACAATGTGT CATTTTCTGT AACTCTCGTA AGAAGGTTGA ATATTTGGCT GAACAGCTTA ATAAGAGAGA CTTTACTGTC TCATCCATGC ACGGCGACAT GGAACCTAAG GATCGTGAAT TGATTATGCA CGAATTCAGA ACTAGTGCCT CTCGTGTTCT CATTACCACT GACTTGTTGG CTCGTGGTAT TGACGTCCAC CATGTTTCCC TTGTCATCAA CTATGATTTG CCACGTCACA AAGAAAACTA TCTCCACAGA ATTGGTAGAT CTGGAAGATA TGGTCGTAAA GGAGTTGCCA TCAACTTCAT CACTAAGGAT GACATTCGTA GTATGAGAGA AATTGAAAAG TTTTACAGTA CCTCTATCGA AGAATGTCCT CAAAATATTC ATGAACTTAT C
Details for NafoA.01586.a.B1.GE40542
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV NNDITVTVDD DYKQKHGKAR YEQKEVKEFD DMNLKEDLLR GIYSYGFEKP SIIQQRAILP ITTSEPPADV IGQAQSGTGK TATFTISMLQ RLNISPEVTQ PQGLVLAPTR ELAQQIHKVI MSLGEYMKVK VHACVGGTKV QHDIAILEEG VHIIVGTPGR VFHMIQSGHL DVSGIKMLVI DEADEMLSRG FKDQIYAIFK NLPQDMQVCL FSATMPADVL EITDKFMRDP IRILVKKEEL TLEGIKQFYI MCEKEDYKLD TLCDLYKILT ISQCVIFCNS RKKVEYLAEQ LNKRDFTVSS MHGDMEPKDR ELIMHEFRTS ASRVLITTDL LARGIDVHHV SLVINYDLPR HKENYLHRIG RSGRYGRKGV AINFITKDDI RSMREIEKFY STSIEEC
Validated NT Sequence
atggctcacc accaccacca ccatatggtt aataacgata tcactgttac tgtcgatgat gactacaagc aaaagcacgg aaaggcaaga tatgaacaaa aggaagtcaa agaatttgat gatatgaatc tcaaggaaga cttgttgaga ggaatttact cttacggttt tgaaaagcct tctatcatcc aacaacgtgc tattttgccc atcactacct ctgaaccacc agcagatgtt atcggtcaag cccaatctgg taccggtaag actgctacct tcactatttc catgcttcaa agattgaata tttctcctga agtaacacaa ccacaaggac tcgtgttggc tccaacccgt gagttggcac aacaaatcca caaggtaatc atgtccttgg gtgaatacat gaaagttaag gttcacgctt gtgtcggtgg tactaaagta caacacgata ttgctattct tgaagaagga gtgcatatta ttgttggtac tcctggacgt gtcttccaca tgattcaaag tggacacttg gatgttagcg gtattaaaat gttggtcatt gatgaagctg acgaaatgtt gagtcgtggt ttcaaggacc aaatctatgc tattttcaag aaccttcctc aagatatgca agtctgtttg ttcagcgcta ctatgcctgc tgatgttttg gaaattactg acaagtttat gagagatcca attagaatct tggtcaagaa ggaagaattg actttggaag gtatcaagca attctatatt atgtgtgaaa aggaagatta caagttggac acattgtgtg acttgtacaa gattttgacc atttcacaat gtgtcatttt ctgtaactct cgtaagaagg ttgaatattt ggctgaacag cttaataaga gagactttac tgtctcatcc atgcacggcg acatggaacc taaggatcgt gaattgatta tgcacgaatt cagaactagt gcctctcgtg ttctcattac cactgacttg ttggctcgtg gtattgacgt ccaccatgtt tcccttgtca tcaactatga tttgccacgt cacaaagaaa actatctcca cagaattggt agatctggaa gatatggtcg taaaggagtt gccatcaact tcatcactaa ggatgacatt cgtagtatga gagaaattga aaagttttac agtacctcta tcgaagaatg tc
Expected Protein Sequence
MAHHHHHHMV NNDITVTVDD DYKQKHGKAR YEQKEVKEFD DMNLKEDLLR GIYSYGFEKP SIIQQRAILP ITTSEPPADV IGQAQSGTGK TATFTISMLQ RLNISPEVTQ PQGLVLAPTR ELAQQIHKVI MSLGEYMKVK VHACVGGTKV QHDIAILEEG VHIIVGTPGR VFHMIQSGHL DVSGIKMLVI DEADEMLSRG FKDQIYAIFK NLPQDMQVCL FSATMPADVL EITDKFMRDP IRILVKKEEL TLEGIKQFYI MCEKEDYKLD TLCDLYKILT ISQCVIFCNS RKKVEYLAEQ LNKRDFTVSS MHGDMEPKDR ELIMHEFRTS ASRVLITTDL LARGIDVHHV SLVINYDLPR HKENYLHRIG RSGRYGRKGV AINFITKDDI RSMREIEKFY STSIEECPQN IHELI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtta ataacgatat cactgttact gtcgatgatg actacaagca aaagcacgga aaggcaagat atgaacaaaa ggaagtcaaa gaatttgatg atatgaatct caaggaagac ttgttgagag gaatttactc ttacggtttt gaaaagcctt ctatcatcca acaacgtgct attttgccca tcactacctc tgaaccacca gcagatgtta tcggtcaagc ccaatctggt accggtaaga ctgctacctt cactatttcc atgcttcaaa gattgaatat ttctcctgaa gtaacacaac cacaaggact cgtgttggct ccaacccgtg agttggcaca acaaatccac aaggtaatca tgtccttggg tgaatacatg aaagttaagg ttcacgcttg tgtcggtggt actaaagtac aacacgatat tgctattctt gaagaaggag tgcatattat tgttggtact cctggacgtg tcttccacat gattcaaagt ggacacttgg atgttagcgg tattaaaatg ttggtcattg atgaagctga cgaaatgttg agtcgtggtt tcaaggacca aatctatgct attttcaaga accttcctca agatatgcaa gtctgtttgt tcagcgctac tatgcctgct gatgttttgg aaattactga caagtttatg agagatccaa ttagaatctt ggtcaagaag gaagaattga ctttggaagg tatcaagcaa ttctatatta tgtgtgaaaa ggaagattac aagttggaca cattgtgtga cttgtacaag attttgacca tttcacaatg tgtcattttc tgtaactctc gtaagaaggt tgaatatttg gctgaacagc ttaataagag agactttact gtctcatcca tgcacggcga catggaacct aaggatcgtg aattgattat gcacgaattc agaactagtg cctctcgtgt tctcattacc actgacttgt tggctcgtgg tattgacgtc caccatgttt cccttgtcat caactatgat ttgccacgtc acaaagaaaa ctatctccac agaattggta gatctggaag atatggtcgt aaaggagttg ccatcaactt catcactaag gatgacattc gtagtatgag agaaattgaa aagttttaca gtacctctat cgaagaatgt cctcaaaata ttcatgaact tatctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NafoA.01586.a.B1.PW37978
PURIFICATION DATe: 11/15/2016
CONCENTRATION: 35.5mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV NNDITVTVDD DYKQKHGKAR YEQKEVKEFD DMNLKEDLLR GIYSYGFEKP SIIQQRAILP ITTSEPPADV IGQAQSGTGK TATFTISMLQ RLNISPEVTQ PQGLVLAPTR ELAQQIHKVI MSLGEYMKVK VHACVGGTKV QHDIAILEEG VHIIVGTPGR VFHMIQSGHL DVSGIKMLVI DEADEMLSRG FKDQIYAIFK NLPQDMQVCL FSATMPADVL EITDKFMRDP IRILVKKEEL TLEGIKQFYI MCEKEDYKLD TLCDLYKILT ISQCVIFCNS RKKVEYLAEQ LNKRDFTVSS MHGDMEPKDR ELIMHEFRTS ASRVLITTDL LARGIDVHHV SLVINYDLPR HKENYLHRIG RSGRYGRKGV AINFITKDDI RSMREIEKFY STSIEEC
Validated NT Sequence
atggctcacc accaccacca ccatatggtt aataacgata tcactgttac tgtcgatgat gactacaagc aaaagcacgg aaaggcaaga tatgaacaaa aggaagtcaa agaatttgat gatatgaatc tcaaggaaga cttgttgaga ggaatttact cttacggttt tgaaaagcct tctatcatcc aacaacgtgc tattttgccc atcactacct ctgaaccacc agcagatgtt atcggtcaag cccaatctgg taccggtaag actgctacct tcactatttc catgcttcaa agattgaata tttctcctga agtaacacaa ccacaaggac tcgtgttggc tccaacccgt gagttggcac aacaaatcca caaggtaatc atgtccttgg gtgaatacat gaaagttaag gttcacgctt gtgtcggtgg tactaaagta caacacgata ttgctattct tgaagaagga gtgcatatta ttgttggtac tcctggacgt gtcttccaca tgattcaaag tggacacttg gatgttagcg gtattaaaat gttggtcatt gatgaagctg acgaaatgtt gagtcgtggt ttcaaggacc aaatctatgc tattttcaag aaccttcctc aagatatgca agtctgtttg ttcagcgcta ctatgcctgc tgatgttttg gaaattactg acaagtttat gagagatcca attagaatct tggtcaagaa ggaagaattg actttggaag gtatcaagca attctatatt atgtgtgaaa aggaagatta caagttggac acattgtgtg acttgtacaa gattttgacc atttcacaat gtgtcatttt ctgtaactct cgtaagaagg ttgaatattt ggctgaacag cttaataaga gagactttac tgtctcatcc atgcacggcg acatggaacc taaggatcgt gaattgatta tgcacgaatt cagaactagt gcctctcgtg ttctcattac cactgacttg ttggctcgtg gtattgacgt ccaccatgtt tcccttgtca tcaactatga tttgccacgt cacaaagaaa actatctcca cagaattggt agatctggaa gatatggtcg taaaggagtt gccatcaact tcatcactaa ggatgacatt cgtagtatga gagaaattga aaagttttac agtacctcta tcgaagaatg tc
Expressed Protein Sequence
MAHHHHHHMV NNDITVTVDD DYKQKHGKAR YEQKEVKEFD DMNLKEDLLR GIYSYGFEKP SIIQQRAILP ITTSEPPADV IGQAQSGTGK TATFTISMLQ RLNISPEVTQ PQGLVLAPTR ELAQQIHKVI MSLGEYMKVK VHACVGGTKV QHDIAILEEG VHIIVGTPGR VFHMIQSGHL DVSGIKMLVI DEADEMLSRG FKDQIYAIFK NLPQDMQVCL FSATMPADVL EITDKFMRDP IRILVKKEEL TLEGIKQFYI MCEKEDYKLD TLCDLYKILT ISQCVIFCNS RKKVEYLAEQ LNKRDFTVSS MHGDMEPKDR ELIMHEFRTS ASRVLITTDL LARGIDVHHV SLVINYDLPR HKENYLHRIG RSGRYGRKGV AINFITKDDI RSMREIEKFY STSIEECPQN IHELI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTTA ATAACGATAT CACTGTTACT GTCGATGATG ACTACAAGCA AAAGCACGGA AAGGCAAGAT ATGAACAAAA GGAAGTCAAA GAATTTGATG ATATGAATCT CAAGGAAGAC TTGTTGAGAG GAATTTACTC TTACGGTTTT GAAAAGCCTT CTATCATCCA ACAACGTGCT ATTTTGCCCA TCACTACCTC TGAACCACCA GCAGATGTTA TCGGTCAAGC CCAATCTGGT ACCGGTAAGA CTGCTACCTT CACTATTTCC ATGCTTCAAA GATTGAATAT TTCTCCTGAA GTAACACAAC CACAAGGACT CGTGTTGGCT CCAACCCGTG AGTTGGCACA ACAAATCCAC AAGGTAATCA TGTCCTTGGG TGAATACATG AAAGTTAAGG TTCACGCTTG TGTCGGTGGT ACTAAAGTAC AACACGATAT TGCTATTCTT GAAGAAGGAG TGCATATTAT TGTTGGTACT CCTGGACGTG TCTTCCACAT GATTCAAAGT GGACACTTGG ATGTTAGCGG TATTAAAATG TTGGTCATTG ATGAAGCTGA CGAAATGTTG AGTCGTGGTT TCAAGGACCA AATCTATGCT ATTTTCAAGA ACCTTCCTCA AGATATGCAA GTCTGTTTGT TCAGCGCTAC TATGCCTGCT GATGTTTTGG AAATTACTGA CAAGTTTATG AGAGATCCAA TTAGAATCTT GGTCAAGAAG GAAGAATTGA CTTTGGAAGG TATCAAGCAA TTCTATATTA TGTGTGAAAA GGAAGATTAC AAGTTGGACA CATTGTGTGA CTTGTACAAG ATTTTGACCA TTTCACAATG TGTCATTTTC TGTAACTCTC GTAAGAAGGT TGAATATTTG GCTGAACAGC TTAATAAGAG AGACTTTACT GTCTCATCCA TGCACGGCGA CATGGAACCT AAGGATCGTG AATTGATTAT GCACGAATTC AGAACTAGTG CCTCTCGTGT TCTCATTACC ACTGACTTGT TGGCTCGTGG TATTGACGTC CACCATGTTT CCCTTGTCAT CAACTATGAT TTGCCACGTC ACAAAGAAAA CTATCTCCAC AGAATTGGTA GATCTGGAAG ATATGGTCGT AAAGGAGTTG CCATCAACTT CATCACTAAG GATGACATTC GTAGTATGAG AGAAATTGAA AAGTTTTACA GTACCTCTAT CGAAGAATGT CCTCAAAATA TTCATGAACT TATCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT