SSGCID: Target Page

NafoA.01549.a

pyruvate phosphate dikinase

CENTER ID:	NafoA.01549.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.01549.a.B1.GE40562	Full length(NafoA.01549.a)		1	931

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.01549.a.B1.PW37977	Full length(NafoA.01549.a)		1	931

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0015830

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTNHNNFLS PNNVSTPQKF CYFFGMDPIT KQNLTEGNRN MKDLLGGKGA GLAEMVNLGV PVPPGFTICT EANVLFNKHN NYASNIDLDD EDEMAQRNLS SRNQLPEQIV SEVNEYLARL EKVTNKKFGS LQNENEKILL VSVRSGAAVS MPGMMDTILN LGLCDDNLPI MIKNFGGNER FVWDSYRNLI QIYGDVVMGV KYEHFEENVK RQKQKRGITL DIDLTLEEIK EIVEDHKKTI FEKTDECFPQ DTKQQLFKAI IAVFKSWTNA RAVHYRRIHN IRNLKGTAVN IQSMVFGNIN QHSGTGVCFS RNPATGENKF YGEYLRQAQG EDVVSGVRTP EKIEELANSF PECYKQLLDI SNKLERHFKD IQDMEFTIEE GVLYMLQCRN GKKAVKAALK IYCDMLDEGL INEKEVILKL EASQLDQLLH RYLSEKEIAK HEPLACGLAA SPGCAVGRIA FTSEAAIEMA KERKTKGPVV LVRTDTSPED LAGMQISQGF FTTNGGITSH AAVVARQNGK CCVSGCSALI VNEHEKTCTI NGVEFKEGDI ITIDGSTGLI FAGALETSEP SLSGDFARVM EIEKRYRTMG VYANADTVGD ATKAIEFGAE GIGLCRTEHM FFEKTRILDM RRMIVATNEK QRAEALQKLK EYQRTDFKGL FDVMGDRKVT IRLLDPPLHE FLPHEEHEVE ELAKKLGEDK SVVMRKIEEL SEKNPMLGHR GCRLGITLPK ITQMQAEAIF EAALTSQSPC VHVEIMIPLI SSIQELKHQK EIIEKVASKV LTHAGHEKVK FRVGCMIETP RAALLADEIA TVSDFFSVGS NDLTQMTFGF SRDDAEKFLK DYIDMELSQT DPFQILDQDG VGKLIRMAVE KGRSVKPDLT VGLCGEHGGE AQSVQFLHTC GLTYVSCSPY RIPIARIAAA QQSILSGSVK K

NT Sequence

ATGAGTACCA ATCACAATAA CTTCCTTTCT CCCAACAATG TGTCCACTCC ACAAAAGTTT TGCTACTTTT TCGGAATGGA TCCCATCACC AAACAAAACC TCACCGAAGG AAATCGAAAT ATGAAAGATC TGTTGGGAGG AAAGGGTGCT GGTCTCGCTG AAATGGTCAA TTTGGGTGTA CCTGTTCCAC CTGGTTTTAC CATTTGCACG GAGGCCAATG TGTTGTTTAA CAAACACAAC AATTATGCTT CCAATATTGA TTTGGATGAT GAAGACGAGA TGGCACAGAG AAATCTCTCT TCAAGAAATC AATTACCTGA ACAAATTGTT TCCGAAGTGA ATGAATATTT GGCAAGATTG GAAAAGGTGA CCAACAAGAA ATTTGGATCT TTGCAAAACG AGAATGAAAA GATCTTGTTG GTTTCTGTCA GAAGTGGTGC TGCTGTCTCC ATGCCAGGAA TGATGGACAC AATTTTGAAT TTGGGTCTTT GTGATGACAA TTTGCCAATC ATGATTAAAA ATTTCGGTGG CAATGAGAGA TTTGTTTGGG ACTCGTACCG TAACTTGATT CAAATCTACG GTGATGTTGT AATGGGAGTC AAGTATGAAC ATTTCGAAGA AAATGTGAAA AGACAAAAAC AAAAGAGAGG CATTACTCTT GACATTGATT TGACTTTGGA AGAAATTAAG GAAATTGTGG AAGATCACAA GAAGACCATT TTCGAGAAAA CAGACGAGTG CTTCCCTCAA GACACTAAGC AACAATTATT CAAGGCCATT ATTGCAGTCT TCAAATCATG GACCAATGCA AGAGCGGTTC ACTACCGAAG AATTCACAAC ATTCGAAATT TGAAAGGAAC TGCTGTCAAT ATTCAATCAA TGGTTTTCGG TAACATTAAC CAACATTCAG GAACTGGTGT ATGCTTTAGT AGAAATCCAG CCACTGGCGA GAACAAATTC TACGGAGAAT ATTTGAGACA AGCACAAGGA GAGGATGTAG TGAGTGGTGT CAGAACACCT GAAAAGATTG AAGAGTTGGC AAATTCTTTC CCCGAGTGTT ATAAACAACT CTTGGACATT AGCAACAAAC TAGAGAGACA CTTTAAAGAC ATTCAAGATA TGGAATTTAC CATTGAAGAG GGTGTCTTGT ACATGCTCCA ATGTCGAAAC GGAAAGAAGG CCGTGAAAGC AGCTCTCAAA ATTTACTGTG ACATGCTCGA TGAAGGTCTC ATCAATGAGA AGGAAGTCAT CCTCAAGTTG GAGGCTTCCC AATTGGATCA ACTTCTTCAT CGTTATCTCT CTGAGAAGGA GATTGCCAAA CACGAACCAT TGGCATGTGG TTTAGCTGCA TCACCAGGAT GTGCTGTGGG AAGAATCGCT TTCACCTCAG AAGCTGCCAT TGAGATGGCA AAAGAGAGAA AAACAAAGGG ACCTGTGGTT TTGGTTCGTA CCGACACCTC TCCCGAAGAT TTGGCAGGTA TGCAAATTTC ACAAGGATTT TTCACAACCA ATGGAGGAAT TACAAGCCAT GCCGCCGTTG TGGCTCGTCA AAATGGTAAA TGTTGTGTGA GTGGTTGCTC AGCACTCATT GTCAATGAGC ATGAAAAGAC ATGCACCATT AATGGTGTTG AATTTAAGGA AGGTGACATT ATAACCATTG ATGGATCCAC TGGATTGATC TTTGCAGGTG CATTGGAGAC TTCTGAACCT TCGTTGAGTG GTGACTTTGC TCGAGTGATG GAGATTGAAA AGCGTTATCG TACCATGGGA GTGTACGCCA ATGCCGATAC AGTCGGCGAT GCCACAAAAG CCATTGAATT TGGTGCTGAA GGTATTGGTT TGTGCCGAAC CGAACACATG TTCTTTGAAA AGACAAGAAT TCTCGATATG AGAAGAATGA TTGTTGCCAC CAATGAGAAA CAACGAGCAG AAGCACTTCA AAAACTCAAA GAATATCAAC GCACTGATTT TAAGGGACTC TTTGATGTGA TGGGAGATCG AAAGGTCACC ATTCGTTTGT TGGATCCACC TCTCCATGAA TTCTTACCTC ATGAAGAACA TGAAGTTGAA GAGCTCGCCA AAAAGCTCGG TGAAGACAAG AGCGTGGTTA TGAGAAAGAT TGAAGAACTC TCTGAAAAGA ATCCCATGCT TGGTCACAGA GGTTGTCGTT TGGGTATTAC TCTTCCAAAA ATTACTCAAA TGCAAGCAGA GGCTATTTTC GAAGCAGCTC TCACCTCTCA ATCACCATGT GTTCATGTGG AAATTATGAT TCCACTCATC TCCTCTATTC AAGAATTGAA ACATCAAAAA GAGATTATCG AAAAAGTTGC TTCCAAAGTA TTGACACACG CAGGACACGA AAAGGTCAAA TTCCGAGTGG GCTGCATGAT TGAAACGCCT CGGGCAGCAT TGCTAGCCGA TGAAATTGCA ACAGTTTCTG ACTTTTTCAG TGTTGGATCC AATGATTTAA CACAAATGAC TTTTGGATTC AGTCGAGATG ATGCCGAGAA ATTCCTTAAA GATTACATCG ATATGGAATT GTCACAAACA GATCCATTCC AAATTCTAGA TCAAGATGGA GTTGGAAAAT TGATTCGAAT GGCAGTTGAA AAGGGAAGAA GTGTGAAACC TGATTTAACT GTTGGATTGT GTGGAGAACA TGGAGGAGAA GCACAATCCG TGCAATTTTT ACATACATGC GGATTGACCT ATGTGAGTTG TTCACCTTAT CGAATTCCAA TTGCTCGAAT TGCAGCTGCA CAACAATCCA TTCTTTCAGG CTCTGTTAAA AAG

Details for NafoA.01549.a.B1.GE40562

HARVESTED ON:	9/6/2016
SEQUENCED ON:	9/8/2016
EXPECTED MW:	105kDa
OBSERVED MW:	105kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS TNHNNFLSPN NVSTPQKFCY FFGMDPITKQ NLTEGNRNMK DLLGGKGAGL AEMVNLGVPV PPGFTICTEA NVLFNKHNNY ASNIDLDDED EMAQRNLSSR NQLPEQIVSE VNEYLARLEK VTNKKFGSLQ NENEKILLVS VRSGAAVSMP GMMDTILNLG LCDDNLPIMI KNFGGNERFV WDSYRNLIQI YGDVVMGVKY EHFEENVKRQ KQKRGITLDI DLTLEEIKEI VEDHKKTIFE KTDECFPQDT KQQLFKAIIA VFKSWTNARA VHYRRIHNIR NLKGTAVNIQ SMVFGNINQH SGTGVCFSRN PATGENKFYG EYLRQAQGED VVSGVRTPEK IEELANSFPE CYKQLLDISN KLERHFKDIQ DMEFTIEEGV LYMLQCRNGK KAVKAALKIY CDMLDEGLIN EKEVILKLEA SQLDQLLHRY LSEKEIAKHE PLACGLAASP GCAVGRIAFT SEAAIEMAKE RKTKGPVVLV RTDTSPEDLA GMQISQGFFT TNGGITSHAA VVARQNGKCC VSGCSALIVN EHEKTCTING VEFKEGDIIT IDGSTGLIFA GALETSEPSL SGDFARVMEI EKRYRTMGVY ANADTVGDAT KAIEFGAEGI GLCRTEHMFF EKTRILDMRR MIVATNEKQR AEALQKLKEY QRTDFKGLFD VMGDRKVTIR LLDPPLHEFL PHEEHEVEEL AKKLGEDKSV VMRKIEELSE KNPMLGHRGC RLGITLPKIT QMQAEAIFEA ALTSQSPCVH VEIMIPLISS IQELKHQKEI IEKVASKVLT HAGHEKVKFR VGCMIETPRA ALLADEIATV SDFFSVGSND LTQMTFGFSR DDAEKFLKDY IDMELSQTDP FQILDQDGVG KLIRMAVEKG RSVKPDLTVG LCGEHGGEAQ SVQFLHTCGL TYVSCSPYRI PIARIAAAQQ SILSGSVKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta ccaatcacaa taacttcctt tctcccaaca atgtgtccac tccacaaaag ttttgctact ttttcggaat ggatcccatc accaaacaaa acctcaccga aggaaatcga aatatgaaag atctgttggg aggaaagggt gctggtctcg ctgaaatggt caatttgggt gtacctgttc cacctggttt taccatttgc acggaggcca atgtgttgtt taacaaacac aacaattatg cttccaatat tgatttggat gatgaagacg agatggcaca gagaaatctc tcttcaagaa atcaattacc tgaacaaatt gtttccgaag tgaatgaata tttggcaaga ttggaaaagg tgaccaacaa gaaatttgga tctttgcaaa acgagaatga aaagatcttg ttggtttctg tcagaagtgg tgctgctgtc tccatgccag gaatgatgga cacaattttg aatttgggtc tttgtgatga caatttgcca atcatgatta aaaatttcgg tggcaatgag agatttgttt gggactcgta ccgtaacttg attcaaatct acggtgatgt tgtaatggga gtcaagtatg aacatttcga agaaaatgtg aaaagacaaa aacaaaagag aggcattact cttgacattg atttgacttt ggaagaaatt aaggaaattg tggaagatca caagaagacc attttcgaga aaacagacga gtgcttccct caagacacta agcaacaatt attcaaggcc attattgcag tcttcaaatc atggaccaat gcaagagcgg ttcactaccg aagaattcac aacattcgaa atttgaaagg aactgctgtc aatattcaat caatggtttt cggtaacatt aaccaacatt caggaactgg tgtatgcttt agtagaaatc cagccactgg cgagaacaaa ttctacggag aatatttgag acaagcacaa ggagaggatg tagtgagtgg tgtcagaaca cctgaaaaga ttgaagagtt ggcaaattct ttccccgagt gttataaaca actcttggac attagcaaca aactagagag acactttaaa gacattcaag atatggaatt taccattgaa gagggtgtct tgtacatgct ccaatgtcga aacggaaaga aggccgtgaa agcagctctc aaaatttact gtgacatgct cgatgaaggt ctcatcaatg agaaggaagt catcctcaag ttggaggctt cccaattgga tcaacttctt catcgttatc tctctgagaa ggagattgcc aaacacgaac cattggcatg tggtttagct gcatcaccag gatgtgctgt gggaagaatc gctttcacct cagaagctgc cattgagatg gcaaaagaga gaaaaacaaa gggacctgtg gttttggttc gtaccgacac ctctcccgaa gatttggcag gtatgcaaat ttcacaagga tttttcacaa ccaatggagg aattacaagc catgccgccg ttgtggctcg tcaaaatggt aaatgttgtg tgagtggttg ctcagcactc attgtcaatg agcatgaaaa gacatgcacc attaatggtg ttgaatttaa ggaaggtgac attataacca ttgatggatc cactggattg atctttgcag gtgcattgga gacttctgaa ccttcgttga gtggtgactt tgctcgagtg atggagattg aaaagcgtta tcgtaccatg ggagtgtacg ccaatgccga tacagtcggc gatgccacaa aagccattga atttggtgct gaaggtattg gtttgtgccg aaccgaacac atgttctttg aaaagacaag aattctcgat atgagaagaa tgattgttgc caccaatgag aaacaacgag cagaagcact tcaaaaactc aaagaatatc aacgcactga ttttaaggga ctctttgatg tgatgggaga tcgaaaggtc accattcgtt tgttggatcc acctctccat gaattcttac ctcatgaaga acatgaagtt gaagagctcg ccaaaaagct cggtgaagac aagagcgtgg ttatgagaaa gattgaagaa ctctctgaaa agaatcccat gcttggtcac agaggttgtc gtttgggtat tactcttcca aaaattactc aaatgcaagc agaggctatt ttcgaagcag ctctcacctc tcaatcacca tgtgttcatg tggaaattat gattccactc atctcctcta ttcaagaatt gaaacatcaa aaagagatta tcgaaaaagt tgcttccaaa gtattgacac acgcaggaca cgaaaaggtc aaattccgag tgggctgcat gattgaaacg cctcgggcag cattgctagc cgatgaaatt gcaacagttt ctgacttttt cagtgttgga tccaatgatt taacacaaat gacttttgga ttcagtcgag atgatgccga gaaattcctt aaagattaca tcgatatgga attgtcacaa acagatccat tccaaattct agatcaagat ggagttggaa aattgattcg aatggcagtt gaaaagggaa gaagtgtgaa acctgattta actgttggat tgtgtggaga acatggagga gaagcacaat ccgtgcaatt tttacataca tgcggattga cctatgtgag ttgttcacct tatcgaattc caattgctcg aattgcagct gcacaacaat ccattctttc aggctctgtt aaaaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for NafoA.01549.a.B1.PW37977

PURIFICATION DATe:	11/15/2016
CONCENTRATION:	26mg/ml
OBSERVED MW:	115kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA CCAATCACAA TAACTTCCTT TCTCCCAACA ATGTGTCCAC TCCACAAAAG TTTTGCTACT TTTTCGGAAT GGATCCCATC ACCAAACAAA ACCTCACCGA AGGAAATCGA AATATGAAAG ATCTGTTGGG AGGAAAGGGT GCTGGTCTCG CTGAAATGGT CAATTTGGGT GTACCTGTTC CACCTGGTTT TACCATTTGC ACGGAGGCCA ATGTGTTGTT TAACAAACAC AACAATTATG CTTCCAATAT TGATTTGGAT GATGAAGACG AGATGGCACA GAGAAATCTC TCTTCAAGAA ATCAATTACC TGAACAAATT GTTTCCGAAG TGAATGAATA TTTGGCAAGA TTGGAAAAGG TGACCAACAA GAAATTTGGA TCTTTGCAAA ACGAGAATGA AAAGATCTTG TTGGTTTCTG TCAGAAGTGG TGCTGCTGTC TCCATGCCAG GAATGATGGA CACAATTTTG AATTTGGGTC TTTGTGATGA CAATTTGCCA ATCATGATTA AAAATTTCGG TGGCAATGAG AGATTTGTTT GGGACTCGTA CCGTAACTTG ATTCAAATCT ACGGTGATGT TGTAATGGGA GTCAAGTATG AACATTTCGA AGAAAATGTG AAAAGACAAA AACAAAAGAG AGGCATTACT CTTGACATTG ATTTGACTTT GGAAGAAATT AAGGAAATTG TGGAAGATCA CAAGAAGACC ATTTTCGAGA AAACAGACGA GTGCTTCCCT CAAGACACTA AGCAACAATT ATTCAAGGCC ATTATTGCAG TCTTCAAATC ATGGACCAAT GCAAGAGCGG TTCACTACCG AAGAATTCAC AACATTCGAA ATTTGAAAGG AACTGCTGTC AATATTCAAT CAATGGTTTT CGGTAACATT AACCAACATT CAGGAACTGG TGTATGCTTT AGTAGAAATC CAGCCACTGG CGAGAACAAA TTCTACGGAG AATATTTGAG ACAAGCACAA GGAGAGGATG TAGTGAGTGG TGTCAGAACA CCTGAAAAGA TTGAAGAGTT GGCAAATTCT TTCCCCGAGT GTTATAAACA ACTCTTGGAC ATTAGCAACA AACTAGAGAG ACACTTTAAA GACATTCAAG ATATGGAATT TACCATTGAA GAGGGTGTCT TGTACATGCT CCAATGTCGA AACGGAAAGA AGGCCGTGAA AGCAGCTCTC AAAATTTACT GTGACATGCT CGATGAAGGT CTCATCAATG AGAAGGAAGT CATCCTCAAG TTGGAGGCTT CCCAATTGGA TCAACTTCTT CATCGTTATC TCTCTGAGAA GGAGATTGCC AAACACGAAC CATTGGCATG TGGTTTAGCT GCATCACCAG GATGTGCTGT GGGAAGAATC GCTTTCACCT CAGAAGCTGC CATTGAGATG GCAAAAGAGA GAAAAACAAA GGGACCTGTG GTTTTGGTTC GTACCGACAC CTCTCCCGAA GATTTGGCAG GTATGCAAAT TTCACAAGGA TTTTTCACAA CCAATGGAGG AATTACAAGC CATGCCGCCG TTGTGGCTCG TCAAAATGGT AAATGTTGTG TGAGTGGTTG CTCAGCACTC ATTGTCAATG AGCATGAAAA GACATGCACC ATTAATGGTG TTGAATTTAA GGAAGGTGAC ATTATAACCA TTGATGGATC CACTGGATTG ATCTTTGCAG GTGCATTGGA GACTTCTGAA CCTTCGTTGA GTGGTGACTT TGCTCGAGTG ATGGAGATTG AAAAGCGTTA TCGTACCATG GGAGTGTACG CCAATGCCGA TACAGTCGGC GATGCCACAA AAGCCATTGA ATTTGGTGCT GAAGGTATTG GTTTGTGCCG AACCGAACAC ATGTTCTTTG AAAAGACAAG AATTCTCGAT ATGAGAAGAA TGATTGTTGC CACCAATGAG AAACAACGAG CAGAAGCACT TCAAAAACTC AAAGAATATC AACGCACTGA TTTTAAGGGA CTCTTTGATG TGATGGGAGA TCGAAAGGTC ACCATTCGTT TGTTGGATCC ACCTCTCCAT GAATTCTTAC CTCATGAAGA ACATGAAGTT GAAGAGCTCG CCAAAAAGCT CGGTGAAGAC AAGAGCGTGG TTATGAGAAA GATTGAAGAA CTCTCTGAAA AGAATCCCAT GCTTGGTCAC AGAGGTTGTC GTTTGGGTAT TACTCTTCCA AAAATTACTC AAATGCAAGC AGAGGCTATT TTCGAAGCAG CTCTCACCTC TCAATCACCA TGTGTTCATG TGGAAATTAT GATTCCACTC ATCTCCTCTA TTCAAGAATT GAAACATCAA AAAGAGATTA TCGAAAAAGT TGCTTCCAAA GTATTGACAC ACGCAGGACA CGAAAAGGTC AAATTCCGAG TGGGCTGCAT GATTGAAACG CCTCGGGCAG CATTGCTAGC CGATGAAATT GCAACAGTTT CTGACTTTTT CAGTGTTGGA TCCAATGATT TAACACAAAT GACTTTTGGA TTCAGTCGAG ATGATGCCGA GAAATTCCTT AAAGATTACA TCGATATGGA ATTGTCACAA ACAGATCCAT TCCAAATTCT AGATCAAGAT GGAGTTGGAA AATTGATTCG AATGGCAGTT GAAAAGGGAA GAAGTGTGAA ACCTGATTTA ACTGTTGGAT TGTGTGGAGA ACATGGAGGA GAAGCACAAT CCGTGCAATT TTTACATACA TGCGGATTGA CCTATGTGAG TTGTTCACCT TATCGAATTC CAATTGCTCG AATTGCAGCT GCACAACAAT CCATTCTTTC AGGCTCTGTT AAAAAGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T