NafoA.01546.a

ras-related c3 botulinum toxin substrate 2

CENTER ID: NafoA.01546.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01546.a.B1.GE40541 Full length(NafoA.01546.a) 1 200
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01546.a.B1.PS38294 Full length(NafoA.01546.a) 1 200

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0055070

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTNIKCVVV GDGAVGKTSM LISYSTDSFP TEYVPTVFDN YCANVMYENH TVSLGLWDTA GQEDYDRLRP LSYPDTEIFI ACFSVVQPSS FTNIKDRWMP ELRKHCPSVP VILCGLKVDM REHDVTLKKL KEQGLHPITK EMGEQLAKEV NCIAYCECSA KTQVGLKECF NLAITAVLHP ERFKNDNKNT KKKDGRCMIL
NT Sequence
ATGGATACCA ATATTAAATG TGTCGTCGTG GGAGATGGAG CCGTTGGTAA GACCTCCATG TTGATTAGTT ATAGTACAGA CTCGTTCCCA ACAGAATATG TGCCAACTGT CTTCGATAAC TATTGCGCCA ATGTCATGTA CGAAAATCAT ACAGTCTCGT TAGGTTTGTG GGATACTGCT GGACAAGAAG ATTACGATCG TTTGCGACCT CTCTCCTATC CAGATACTGA AATTTTCATT GCTTGCTTCT CTGTGGTTCA ACCTTCTTCC TTTACCAATA TTAAGGATCG TTGGATGCCA GAATTACGTA AACATTGTCC ATCTGTTCCA GTAATTTTGT GTGGTTTGAA AGTTGACATG AGAGAACATG ATGTCACACT GAAGAAATTG AAAGAACAAG GTCTTCATCC AATAACAAAA GAAATGGGAG AACAATTGGC AAAAGAAGTG AACTGTATTG CTTATTGTGA ATGTTCTGCT AAGACACAAG TTGGTTTGAA GGAATGTTTC AATTTGGCCA TCACTGCAGT ATTGCATCCT GAGAGATTCA AGAATGATAA TAAGAATACT AAGAAGAAGG ACGGTAGATG CATGATTTTG
Details for NafoA.01546.a.B1.GE40541
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD TNIKCVVVGD GAVGKTSMLI SYSTDSFPTE YVPTVFDNYC ANVMYENHTV SLGLWDTAGQ EDYDRLRPLS YPDTEIFIAC FSVVQPSSFT NIKDRWMPEL RKHCPSVPVI LCGLKVDMRE HDVTLKKLKE QGLHPITKEM GEQLAKEVNC IAYCECSAKT QVGLKECFNL AITAVLHPER FKNDNKNTKK KDGRCMIL
Validated NT Sequence
atggctcacc accaccacca ccatatggat accaatatta aatgtgtcgt cgtgggagat ggagccgttg gtaagacctc catgttgatt agttatagta cagactcgtt cccaacagaa tatgtgccaa ctgtcttcga taactattgc gccaatgtca tgtacgaaaa tcatacagtc tcgttaggtt tgtgggatac tgctggacaa gaagattacg atcgtttgcg acctctctcc tatccagata ctgaaatttt cattgcttgc ttctctgtgg ttcaaccttc ttcctttacc aatattaagg atcgttggat gccagaatta cgtaaacatt gtccatctgt tccagtaatt ttgtgtggtt tgaaagttga catgagagaa catgatgtca cactgaagaa attgaaagaa caaggtcttc atccaataac aaaagaaatg ggagaacaat tggcaaaaga agtgaactgt attgcttatt gtgaatgttc tgctaagaca caagttggtt tgaaggaatg tttcaatttg gccatcactg cagtattgca tcctgagaga ttcaagaatg ataataagaa tactaagaag aaggacggta gatgcatgat tttgtaagtg agtaagat
Expected Protein Sequence
MAHHHHHHMD TNIKCVVVGD GAVGKTSMLI SYSTDSFPTE YVPTVFDNYC ANVMYENHTV SLGLWDTAGQ EDYDRLRPLS YPDTEIFIAC FSVVQPSSFT NIKDRWMPEL RKHCPSVPVI LCGLKVDMRE HDVTLKKLKE QGLHPITKEM GEQLAKEVNC IAYCECSAKT QVGLKECFNL AITAVLHPER FKNDNKNTKK KDGRCMIL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata ccaatattaa atgtgtcgtc gtgggagatg gagccgttgg taagacctcc atgttgatta gttatagtac agactcgttc ccaacagaat atgtgccaac tgtcttcgat aactattgcg ccaatgtcat gtacgaaaat catacagtct cgttaggttt gtgggatact gctggacaag aagattacga tcgtttgcga cctctctcct atccagatac tgaaattttc attgcttgct tctctgtggt tcaaccttct tcctttacca atattaagga tcgttggatg ccagaattac gtaaacattg tccatctgtt ccagtaattt tgtgtggttt gaaagttgac atgagagaac atgatgtcac actgaagaaa ttgaaagaac aaggtcttca tccaataaca aaagaaatgg gagaacaatt ggcaaaagaa gtgaactgta ttgcttattg tgaatgttct gctaagacac aagttggttt gaaggaatgt ttcaatttgg ccatcactgc agtattgcat cctgagagat tcaagaatga taataagaat actaagaaga aggacggtag atgcatgatt ttgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.01546.a.B1.PS38294
PURIFICATION DATe: 5/24/2017
CONCENTRATION: 30.7mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD TNIKCVVVGD GAVGKTSMLI SYSTDSFPTE YVPTVFDNYC ANVMYENHTV SLGLWDTAGQ EDYDRLRPLS YPDTEIFIAC FSVVQPSSFT NIKDRWMPEL RKHCPSVPVI LCGLKVDMRE HDVTLKKLKE QGLHPITKEM GEQLAKEVNC IAYCECSAKT QVGLKECFNL AITAVLHPER FKNDNKNTKK KDGRCMIL
Validated NT Sequence
atggctcacc accaccacca ccatatggat accaatatta aatgtgtcgt cgtgggagat ggagccgttg gtaagacctc catgttgatt agttatagta cagactcgtt cccaacagaa tatgtgccaa ctgtcttcga taactattgc gccaatgtca tgtacgaaaa tcatacagtc tcgttaggtt tgtgggatac tgctggacaa gaagattacg atcgtttgcg acctctctcc tatccagata ctgaaatttt cattgcttgc ttctctgtgg ttcaaccttc ttcctttacc aatattaagg atcgttggat gccagaatta cgtaaacatt gtccatctgt tccagtaatt ttgtgtggtt tgaaagttga catgagagaa catgatgtca cactgaagaa attgaaagaa caaggtcttc atccaataac aaaagaaatg ggagaacaat tggcaaaaga agtgaactgt attgcttatt gtgaatgttc tgctaagaca caagttggtt tgaaggaatg tttcaatttg gccatcactg cagtattgca tcctgagaga ttcaagaatg ataataagaa tactaagaag aaggacggta gatgcatgat tttgtaagtg agtaagat
Expressed Protein Sequence
MAHHHHHHMD TNIKCVVVGD GAVGKTSMLI SYSTDSFPTE YVPTVFDNYC ANVMYENHTV SLGLWDTAGQ EDYDRLRPLS YPDTEIFIAC FSVVQPSSFT NIKDRWMPEL RKHCPSVPVI LCGLKVDMRE HDVTLKKLKE QGLHPITKEM GEQLAKEVNC IAYCECSAKT QVGLKECFNL AITAVLHPER FKNDNKNTKK KDGRCMIL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATA CCAATATTAA ATGTGTCGTC GTGGGAGATG GAGCCGTTGG TAAGACCTCC ATGTTGATTA GTTATAGTAC AGACTCGTTC CCAACAGAAT ATGTGCCAAC TGTCTTCGAT AACTATTGCG CCAATGTCAT GTACGAAAAT CATACAGTCT CGTTAGGTTT GTGGGATACT GCTGGACAAG AAGATTACGA TCGTTTGCGA CCTCTCTCCT ATCCAGATAC TGAAATTTTC ATTGCTTGCT TCTCTGTGGT TCAACCTTCT TCCTTTACCA ATATTAAGGA TCGTTGGATG CCAGAATTAC GTAAACATTG TCCATCTGTT CCAGTAATTT TGTGTGGTTT GAAAGTTGAC ATGAGAGAAC ATGATGTCAC ACTGAAGAAA TTGAAAGAAC AAGGTCTTCA TCCAATAACA AAAGAAATGG GAGAACAATT GGCAAAAGAA GTGAACTGTA TTGCTTATTG TGAATGTTCT GCTAAGACAC AAGTTGGTTT GAAGGAATGT TTCAATTTGG CCATCACTGC AGTATTGCAT CCTGAGAGAT TCAAGAATGA TAATAAGAAT ACTAAGAAGA AGGACGGTAG ATGCATGATT TTGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT